Information on EC 3.4.22.B19 - YopJ protease

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The expected taxonomic range for this enzyme is: Yersinia

EC NUMBER
COMMENTARY hide
3.4.22.B19
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
YopJ protease
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
cleaves reversible post-translational modification in form of ubiquitin or a ubiquitin-like protein. It inhibits the host immune response and induces apoptosis by blocking multiple signaling pathways, including the MAPK and NfkappaB pathways in the infected cell
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
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-
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CAS REGISTRY NUMBER
COMMENTARY hide
355808-07-0
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain 8081v
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-
Manually annotated by BRENDA team
strain Kimberley53
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
metabolism
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
Cdc42 + H2O
?
show the reaction diagram
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-
-
-
?
IkappaBalpha-ubiquitin + H2O
IkappaBalpha + ubiquitin
show the reaction diagram
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-
-
-
?
Rac1 + H2O
?
show the reaction diagram
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-
-
-
?
RhoA + H2O
?
show the reaction diagram
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-
-
-
?
TRAF2-ubiquitin + H2O
TRAF2 + ubiquitin
show the reaction diagram
TRAF3-ubiquitin + H2O
TRAF3 + ubiquitin
show the reaction diagram
TRAF6-ubiquitin + H2O
TRAF6 + ubiquitin
show the reaction diagram
ubiquitin-7-amido-4-methylcoumarin + H2O
ubiquitin + 7-amino-4-methylcoumarin
show the reaction diagram
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-
-
-
?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
Cdc42 + H2O
?
show the reaction diagram
-
-
-
-
?
IkappaBalpha-ubiquitin + H2O
IkappaBalpha + ubiquitin
show the reaction diagram
-
-
-
-
?
Rac1 + H2O
?
show the reaction diagram
-
-
-
-
?
RhoA + H2O
?
show the reaction diagram
-
-
-
-
?
TRAF2-ubiquitin + H2O
TRAF2 + ubiquitin
show the reaction diagram
TRAF3-ubiquitin + H2O
TRAF3 + ubiquitin
show the reaction diagram
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YopJ is a deubiquitinating protease that acts on TRAF proteins to prevent or remove the K63-polymerized ubiquitin conjugates required for signal transduction
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?
TRAF6-ubiquitin + H2O
TRAF6 + ubiquitin
show the reaction diagram
additional information
?
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.003
ubiquitin-7-amino-4-methylcoumarin
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pH 8.0
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
33000
-
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
wild-type YopJ and YopJ mutant C172A from insect cells
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Schizosaccharomyces pombe
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C139S
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catalytically inactive