Information on EC 3.2.2.1 - purine nucleosidase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea

EC NUMBER
COMMENTARY hide
3.2.2.1
-
RECOMMENDED NAME
GeneOntology No.
purine nucleosidase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
a purine nucleoside + H2O = D-ribose + a purine base
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of N-glycosyl bond
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
adenosine nucleotides degradation I
-
-
guanine and guanosine salvage II
-
-
guanosine nucleotides degradation I
-
-
guanosine nucleotides degradation II
-
-
Metabolic pathways
-
-
Nicotinate and nicotinamide metabolism
-
-
Purine metabolism
-
-
purine metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
purine-nucleoside ribohydrolase
The enzyme from the bacterium Ochrobactrum anthropi specifically catalyses the irreversible N-riboside hydrolysis of purine nucleosides. Pyrimidine nucleosides, purine and pyrimidine nucleotides, NAD+, NADP+ and nicotinaminde mononucleotide are not substrates [6].
CAS REGISTRY NUMBER
COMMENTARY hide
9025-44-9
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
subspecies Bacillus thuringiensis kurstaki
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
isoforms rih1, rih2
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
ssp. ingenoplastis; ssp. muscarum
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
no activity in Anopheles gambiae
salivary gland
-
-
Manually annotated by BRENDA team
no activity in Culex quinquefasciatus
salivary gland
-
-
Manually annotated by BRENDA team
no activity in Lutzomyia longipalpis
salivary gland
-
-
Manually annotated by BRENDA team
-
E15229
Genbank
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
serovar typhimurium
-
-
Manually annotated by BRENDA team
subsp. brucei
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2'-deoxyadenosine + H2O
D-ribose + ?
show the reaction diagram
-
-
-
-
-
2,6-diamino-9-beta-D-ribofuranosyladenine + H2O
2,6-diaminoadenine + D-ribose
show the reaction diagram
-
76% of the activity with inosine
-
-
ir
2,8-diamino-9-beta-D-ribofuranosyladenine + H2O
2,8-diaminoadenine + D-ribose
show the reaction diagram
-
38% of the activity with inosine
-
-
ir
2-aminopurine riboside + H2O
2-aminopurine + D-ribose
show the reaction diagram
2-hydroxy-9-beta-D-ribofuranosyladenine + H2O
2-hydroxyadenine + D-ribose
show the reaction diagram
-
10% of the activity with inosine
-
-
ir
3'-AMP + H2O
adenine + D-ribose 3-phosphate
show the reaction diagram
E15229
0.67% of the activity with adenosine
-
-
?
3'-deoxyadenosine + H2O
D-ribose + ?
show the reaction diagram
-
-
-
-
-
3-deazaadenosine + H2O
D-ribose + 3-deazaadenine
show the reaction diagram
-
-
-
-
?
4-nitrophenyl beta-D-ribofuranoside + H2O
4-nitrophenol + beta-D-ribofuranose
show the reaction diagram
-
-
-
-
?
4-nitrophenyl beta-D-ribofuranoside + H2O
4-nitrophenol + D-ribose
show the reaction diagram
-
-
-
-
?
4-nitrophenylriboside + H2O
4-nitrophenol + D-ribose
show the reaction diagram
-
-
-
-
?
5'-AMP + H2O
adenine + D-ribose 5-phosphate
show the reaction diagram
-
-
-
-
?
5'-deoxyadenosine + H2O
5-deoxy-D-ribose + adenine
show the reaction diagram
-
-
-
-
?
5'-deoxyadenosine + H2O
D-ribose + ?
show the reaction diagram
-
-
-
-
-
5'-GMP + H2O
guanine + D-ribose 5-phosphate
show the reaction diagram
5'-IMP + H2O
hypoxanthine + D-ribose 5-phosphate
show the reaction diagram
-
-
-
-
?
5,6-dimethyl-1-beta-D-ribofuranosylbenzimidazole + H2O
5,6-dimethylbenzimidazole + D-ribose
show the reaction diagram
-
200% of the activity with inosine
-
-
ir
5-methyluridine + H2O
5-methyluracil + D-ribose
show the reaction diagram
6-dimethylamino-9-beta-D-ribofuranosylbenzimidazole + H2O
6-dimethylaminobenzimidazole + D-ribose
show the reaction diagram
-
30% of the activity with inosine
-
-
ir
6-mercaptopurine ribonucleoside + H2O
6-mercaptopurine + ribose
show the reaction diagram
-
-
-
-
?
7-methyl guanosine + H2O
D-ribose + 7-methyl guanine
show the reaction diagram
-
-
-
-
?
7-methylguanosine + H2O
?
show the reaction diagram
-
-
-
-
?
9-beta-D-ribofuranosylpurine + H2O
purine + D-ribose
show the reaction diagram
-
47% of the activity with inosine
-
-
ir
adenosine + H2O
adenine + D-ribose
show the reaction diagram
adenosine + H2O
D-ribose + adenine
show the reaction diagram
adenosine 5'-phosphate + H2O
?
show the reaction diagram
-
31% of the activity with inosine
-
-
?
cytidine + H2O
cytosine + D-ribose
show the reaction diagram
cytidine + H2O
D-ribose + cytosine
show the reaction diagram
deamino-NAD+ + H2O
?
show the reaction diagram
-
-
-
-
?
ethenoadenosine + H2O
D-ribose + ethenoadenine
show the reaction diagram
-
-
-
-
?
guanosine + H2O
D-ribose + guanine
show the reaction diagram
guanosine + H2O
D-ribose + xanthine
show the reaction diagram
-
-
-
-
?
guanosine + H2O
guanine + D-ribose
show the reaction diagram
imidazoleacetic acid riboside + H2O
imidazoleacetate + D-ribose
show the reaction diagram
-
5% of the activity with inosine
-
-
ir
inosine + H2O
D-ribose + hypoxanthine
show the reaction diagram
inosine + H2O
hypoxanthine + D-ribose
show the reaction diagram
NAD+ + H2O
adenine + ?
show the reaction diagram
-
-
-
?
nicotinamide riboside + H2O
nicotinamide + D-ribose
show the reaction diagram
-
150% of the activity with inosine
-
-
ir
p-nitrophenylriboside + H2O
4-nitrophenol + D-ribose
show the reaction diagram
-
-
-
-
-
p-nitrophenylriboside + H2O
p-nitrophenol + D-ribose
show the reaction diagram
-
-
-
?
purine ribonucleoside + H2O
purine + D-ribose
show the reaction diagram
purine riboside + H2O
purine + D-ribose
show the reaction diagram
thymidine + H2O
D-ribose + thymine
show the reaction diagram
-
-
-
-
?
uridine + H2O
D-ribose + uracil
show the reaction diagram
uridine + H2O
uracil + D-ribose
show the reaction diagram
xanthosine + H2O
D-ribose + xanthine
show the reaction diagram
xanthosine + H2O
xanthine + D-ribose
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
guanosine + H2O
D-ribose + guanine
show the reaction diagram
-
-
-
-
?
inosine + H2O
D-ribose + hypoxanthine
show the reaction diagram
-
-
-
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
CaCl2
-
slight activation
Co2+
E15229
3 mM, reactivation after preincubation with 8-hydroxyquinoline
Mg2+
-
increases activity
MnCl2
-
slight activation
Zn2+
E15229
3 mM, reactivation after preincubation with 8-hydroxyquinoline
additional information
-
a metalloenzyme
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(+-)-1-[(9-deazahypoxanthin-9-yl)methyl]azetidine-2-methanol
(1S)-(1-aminonaphthalen-4-yl)-1,4-dideoxy-1,4-imino-D-ribitol
-
-
(1S)-1,4-dideoxy-1-(2-guanidinoethyl)-1,4-imino-D-ribitol
-
-
(1S)-1-(2,3-diamino-5-pyridyl)-1,4-dideoxy-1,4-imino-D-ribitol
-
-
(1S)-1-(3,4-diaminophenyl)-1,4-dideoxy-1,4-imino-D-ribitol
-
-
(1S)-1-(4-aminomethyl)phenyl-1,4-dideoxy-1,4-imino-D-ribitol
-
-
(1S)-1-(4-cyanophenyl)1,4-dideoxy-1,4-imino-D-ribitol
-
-
(2R,3R,4S)-1-benzyl-2-(hydroxymethyl)pyrrolidine-3,4-diol
-
-
(2R,3R,4S)-1-[(4-amino-4,5-dihydro-3H-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-2-(hydroxymethyl)pyrrolidine-3,4-diol
-
-
(2R,3R,4S)-1-[(4-hydroxy-5H-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-2-(hydroxymethyl)pyrrolidin-3,4-diol
-
nanomolar inhibitor
(2R,3R,4S)-2-(hydroxymethyl)-1-(4-iodobenzyl)pyrrolidine-3,4-diol
-
-
(2R,3R,4S)-2-(hydroxymethyl)-1-(4-nitrobenzyl)pyrrolidine-3,4-diol
-
-
(2R,3R,4S)-2-(hydroxymethyl)-1-(quinolin-8-ylmethyl)pyrrolidin-3,4-diol
-
nanomolar inhibitor
(2R,3R,4S)-2-(hydroxymethyl)-1-(quinolin-8-ylmethyl)pyrrolidine-3,4-diol
-
-
(2R,3R,4S,5S)-2-(hydroxymethyl)-5-phenylpyrrolidine-3,4-diol
-
-
(2S,3R,4S)-1-[(4-amino-5H-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-2-(hydroxymethyl)pyrrolidine-3,4-diol
-
nanomolar inhibitor
1,10-phenanthroline
E15229
1 mM, 23% inhibition
1,2,5-trideoxy-1-(guanin-9-yl)-2,5-imino-D-allitol
-
-
-
1,2,5-trideoxy-1-(hypoxanthin-9-yl)-2,5-imino-D-alitol
-
-
-
1,4-dideoxy-(3-guanidinopropyl)-1,4-imino-D-ribitol
-
-
1,4-dideoxy-1,4-imino-D-ribitol
-
-
1,4-dideoxy-1,4-imino-N-(1-isoquinolinyl)methyl-D-ribitol
-
-
1,4-dideoxy-1,4-imino-N-(1-methyl-1H-1,2,3-benzotriazol-5-yl)methyl-D-ribitol
-
-
1,4-dideoxy-1,4-imino-N-(1-methyl-1H-benzimidazol-2-yl)methyl-D-ribitol
-
-
1,4-dideoxy-1,4-imino-N-(2(1H)-quinolon-8-yl)methyl-D-ribitol
-
-
1,4-dideoxy-1,4-imino-N-(2-amino-4-benzothiazoyl)-methyl-D-ribitol
-
-
1,4-dideoxy-1,4-imino-N-(2-aminopyridin-3-yl)methyl-D-ribitol
-
-
1,4-dideoxy-1,4-imino-N-(2-aminoquinolin-8-yl)methyl-D-ribitol
-
-
1,4-dideoxy-1,4-imino-N-(2-chloroquinolin-8-yl)methyl-D-ribitol
-
-
1,4-dideoxy-1,4-imino-N-(2-methoxyquinolin-8-yl)methyl-D-ribitol
-
-
1,4-dideoxy-1,4-imino-N-(2-naphthyl)methyl-D-ribitol
-
-
1,4-dideoxy-1,4-imino-N-(2-quinolinyl)methyl-D-ribitol
-
-
1,4-dideoxy-1,4-imino-N-(3-cyanobenzyl)-D-ribitol
-
-
1,4-dideoxy-1,4-imino-N-(3-indolyl)methyl-D-ribitol
-
-
1,4-dideoxy-1,4-imino-N-(3-nitrobenzyl)-D-ribitol
-
-
1,4-dideoxy-1,4-imino-N-(3-quinolinyl)methyl-D-ribitol
-
-
1,4-dideoxy-1,4-imino-N-(4(1H)-quinolon-8-yl)methyl-D-ribitol
-
-
1,4-dideoxy-1,4-imino-N-(4-amidinobenzyl)-D-ribitol
-
-
1,4-dideoxy-1,4-imino-N-(4-amino-2-methylquinolin-8-yl)methyl-D-ribitol
-
-
1,4-dideoxy-1,4-imino-N-(4-aminoquinazolin-8-yl)methyl-D-ribitol
-
-
1,4-dideoxy-1,4-imino-N-(4-aminoquinolin-8-yl)methyl-D-ribitol
-
-
1,4-dideoxy-1,4-imino-N-(4-aminothieno[3,2-d]pyrimidin-7-yl)-methyl-D-ribitol
-
-
1,4-dideoxy-1,4-imino-N-(4-chloro-2-methylquinolin-8-yl)methyl-D-ribitol
-
-
1,4-dideoxy-1,4-imino-N-(4-chloroquinolin-8-yl)methyl-D-ribitol
-
-
1,4-dideoxy-1,4-imino-N-(4-cyanobenzyl)-D-ribitol
-
-
1,4-dideoxy-1,4-imino-N-(4-guanidinobenzyl)-D-ribitol
-
-
1,4-dideoxy-1,4-imino-N-(4-methoxyquinolin-8-yl)methyl-D-ribitol
-
-
1,4-dideoxy-1,4-imino-N-(4-methyl-2-phenylpyrimidin-5-yl)methyl-D-ribitol
-
-
1,4-dideoxy-1,4-imino-N-(4-pyridinyl)methyl-D-ribitol
-
-
1,4-dideoxy-1,4-imino-N-(4-quinolinyl)methyl-D-ribitol
-
-
1,4-dideoxy-1,4-imino-N-(6-methoxypyridin-3-yl)methyl-D-ribitol
-
-
1,4-dideoxy-1,4-imino-N-(7-methoxy-indol-3-yl)methyl-D-ribitol
-
-
1,4-dideoxy-1,4-imino-N-(7-quinolinyl)methyl-D-ribitol
-
-
1,4-dideoxy-1,4-imino-N-(7-thieno[3,2-d]pyrimidonyl)-methyl-D-ribitol
-
-
1,4-dideoxy-1,4-imino-N-(8-hydroxyquinolin-2-yl)methyl-D-ribitol
-
-
1,4-dideoxy-1,4-imino-N-(8-quinolinyl)methyl-D-ribitol
-
-
1,4-dideoxy-1,4-imino-N-(quinazolin-8-yl)methyl-D-ribitol
-
-
1,4-dideoxy-1,4-imino-N-(quinazolon-8-yl)methyl-D-ribitol
-
-
1,4-dideoxy-1,4-imino-N-(quinoxalin-5-yl)-methyl-D-ribitol
-
-
1,4-dideoxy-1,4-imino-N-(thieno[3,2-d]pyrimidin-7-yl)methyl-D-ribitol
-
-
1,4-dideoxy-1,4-imino-N-[3-(1H-pyrrol-1-yl)benzyl]-D-ribitol
-
-
1,4-dideoxy-1,4-imino-N-[4-(1H-1,2,4-triazol-1-yl)benzyl]-D-ribitol
-
-
1,4-dideoxy-1,4-imino-N-[4-(1H-pyrazol-1-yl)benzyl]-D-ribitol
-
-
1,4-dideoxy-1,4-imino-N-[4-(1H-pyrrol-1-yl)benzyl]-D-ribitol
-
-
1-(5-carboxamido-2-pyridyl)-1,2,5-trideoxy-2,5-imino-D-allitol
-
-
1-(adenin-9-yl)-1,2,5-trideoxy-2,5-imino-D-allitol
-
-
1-guanidino-2,5-anhydro-1-deoxy-D-allitol
-
-
1-guanidino-3,6-anhydro-1,2-dideoxy-D-allo-heptitol
-
-
1-[(7-benzyloxymethyl-6-tert-butoxy-9-deazapurin-9-yl)methyl] azetidine-3-methanol
1-[(9-deazahypoxanthin-9-yl)methyl]azetidine-3,3dimethanol
1-[2-[(2S,3S,4R,5R)-3,4-dihydroxy-5-(hydroxymethyl)pyrrolidin-2-yl]ethyl]guanidine
-
-
1-[3-[(2R,3R,4S)-3,4-dihydroxy-2-(hydroxymethyl)pyrrolidin-1-yl]propyl]guanidine
-
-
2'-deoxyadenosine
2'-deoxyinosine
-
-
3'-deoxyadenosine
-
-
3-deazaadenosine
4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin H
7-deazaadenosine
7-[[(2R,3R,4S)-3,4-dihydroxy-2-(hydroxymethyl)pyrrolidin-1-yl]methyl]-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
-
8-aza-immucillin A
-
-
8-aza-immucillin H
-
-
8-aza-immucillin-H
-
nanomolar inhibitor
8-hydroxyquinoline
E15229
1 mM, 93% inhibition
adenosine
Ag+
E15229
1 mM, 68% inhibition
Caffeine
-
weak competitive inhibitor
Co2+
-
mixed-type inhibition
cytidine
E15229
0.5 mM, 81% inhibition of activity with adenosine, 92% inhibition of activity with inosine, 91% inhibition of the activity with xanthosine, 94% inhibition of activity with guanosine
D-ribose
daidzein
-
enzyme is a major daidzein-binding protein
formononetin
-
-
formycin B
-
-
GMP
-
substrate inhibition above 1 mM
guanosine
-
competitive inhibitor of xanthosine hydrolysis
Hg2+
E15229
1 mM,61% inhibition
HgCl2
hypoxanthine
hypoxanthine arabinoside
-
-
iminoribitols
-
poorly inhibited by free or substituted iminoribitols
-
immucillin A
immucillin H
immucillin-A
immucillin-H
Inosine
-
competitive inhibitor of xanthosine hydrolysis
iodoacetate
-
0.01 M, 25% inhibition. 0.02 M 36.1% inhibition
KCl
-
slight inhibition
LiSO4
-
inhibits activity with adenosine and guanosine, slight inhibition of activity with inosine
-
meso-[(9-deazahypoxanthin-9-yl)methyl]azetidine-2,4-dimethanol hydrochloride
Methylxanthine
-
weak competitive inhibitor
MnSO4
-
inhibits activity with adenosine and guanosine, very slight inhibition of activity with inosine
N-(9-deaza-adenin-9-yl)methyl-1,4-dideoxy-1,4-imino-D-ribitol
N-(9-deazaadenin-9-yl)methyl-1,4-dideoxy-1,4-imino-D-ribitol
-
-
N-(9-deazahypoxanthin-9-yl)methyl-1,4-dideoxy-1,4-imino-D-ribitol
-
-
N-benzyl-1,4-dideoxy-1,4-imino-D-ribitol
-
-
NaAsO2
E15229
-
Ni2+
-
mixed-type inhibition
purine 2'-deoxyribonucleoside
-
-
pyrazolo(3,4-d)pyrimidine
-
-
UAMC-00109
UAMC-00115
UAMC-00218
UAMC-00311
UAMC-00312
UAMC-00346
UAMC-00363
UAMC-00375
uridine
Xanthosine
Zn2+
-
mixed-type inhibition
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
-
increases activity
GSH
-
increases activity
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.042
2'-deoxyadenosine
-
-
0.0008 - 0.009
2-aminopurine riboside
0.6
3'-deoxyadenosine
-
-
0.0022
3-deazaadenosine
-
at pH 7.0 and 35°C
0.0011
4-nitrophenyl beta-D-ribofuranoside
-
at pH 7.0 and 35°C
2.9
5'-AMP
-
-
0.24 - 9.87
5'-deoxyadenosine
0.026 - 1.3
5-methyluridine
0.0005 - 0.008
7-methyl guanosine
0.001 - 20
adenosine
0.106 - 10
cytidine
4.7
cytosine
-
-
0.0036
ethenoadenosine
-
at pH 7.0 and 35°C
0.909
GMP
-
-
0.0018 - 22.2
guanosine
0.0019 - 1390
Inosine
0.562 - 257
p-nitrophenylriboside
0.004 - 0.011
purine ribonucleoside
0.0006 - 0.145
purine riboside
0.102 - 2.84
uridine
0.0158 - 5.9
Xanthosine
additional information
additional information
substrate specificity and kinetics, overview
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.57
2-aminopurine riboside
0.009
3-deazaadenosine
Trypanosoma brucei brucei
-
at pH 7.0 and 35°C
0.03
4-nitrophenyl beta-D-ribofuranoside
Trypanosoma brucei brucei
-
at pH 7.0 and 35°C
0.078
5'-deoxyadenosine
Trypanosoma brucei brucei
-
at pH 7.0 and 35°C
1.07 - 13.6
7-methyl guanosine
5.2
7-methylguanosine
Trypanosoma brucei brucei
-
at pH 7.0 and 5°C
0.237 - 17.3
adenosine
0.338 - 7.8
cytidine
6.9
ethenoadenosine
Trypanosoma brucei brucei
-
at pH 7.0 and 35°C
0.84 - 37.6
guanosine
0.06 - 61
Inosine
0.206
p-nitrophenylriboside
Trypanosoma vivax
-
pH 7.2, 35°C, His-tagged enzyme form
2 - 3.9
purine ribonucleoside
0.248
purine riboside
Trypanosoma brucei brucei
-
at pH 7.0 and 35°C
0.0022 - 46
uridine
0.029 - 62
Xanthosine
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
700
2-aminopurine riboside
Trypanosoma brucei brucei
-
at pH 7.0 and 35°C
8690
41
3-deazaadenosine
Trypanosoma brucei brucei
-
at pH 7.0 and 35°C
2189
27
4-nitrophenyl beta-D-ribofuranoside
Trypanosoma brucei brucei
-
at pH 7.0 and 35°C
41699
0.33
5'-deoxyadenosine
Trypanosoma brucei brucei
-
at pH 7.0 and 35°C
943
240 - 1090
adenosine
122
1.9
cytidine
Trypanosoma brucei brucei
-
at pH 7.0 and 35°C
372
1900
ethenoadenosine
Trypanosoma brucei brucei
-
at pH 7.0 and 35°C
25599
499 - 8300
guanosine
296
0.1 - 15000
Inosine
167
380
purine riboside
Trypanosoma brucei brucei
-
at pH 7.0 and 35°C
2984
0.0026
uridine
Trypanosoma brucei brucei
-
at pH 7.0 and 35°C
261
0.4 - 61
Xanthosine
1222
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0000018
(+-)-1-[(9-deazahypoxanthin-9-yl)methyl]azetidine-2-methanol
0.018
(1S )-1,4-dideoxy-1-(2-guanidinoethyl)-1,4-imino-D-ribitol
-
pH 7.0, 35°C
0.059
(2R,3R,4S)-1-benzyl-2-(hydroxymethyl)pyrrolidine-3,4-diol
-
-
0.0000041
(2R,3R,4S)-1-[(4-amino-4,5-dihydro-3H-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-2-(hydroxymethyl)pyrrolidine-3,4-diol
-
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.0000044
(2R,3R,4S)-1-[(4-hydroxy-5H-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-2-(hydroxymethyl)pyrrolidin-3,4-diol
-
-
0.0043
(2R,3R,4S)-2-(hydroxymethyl)-1-(4-iodobenzyl)pyrrolidine-3,4-diol
-
-
0.0075
(2R,3R,4S)-2-(hydroxymethyl)-1-(4-nitrobenzyl)pyrrolidine-3,4-diol
-
-
0.0000108
(2R,3R,4S)-2-(hydroxymethyl)-1-(quinolin-8-ylmethyl)pyrrolidin-3,4-diol
-
-
0.0000108
(2R,3R,4S)-2-(hydroxymethyl)-1-(quinolin-8-ylmethyl)pyrrolidine-3,4-diol
-
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.253
(2R,3R,4S,5S)-2-(hydroxymethyl)-5-phenylpyrrolidine-3,4-diol
-
-
0.0000041
(2S,3R,4S)-1-[(4-amino-5H-pyrrolo[3,2-d]pyrimidin-7-yl)methyl]-2-(hydroxymethyl)pyrrolidine-3,4-diol
-
-
0.04
1,4-dideoxy-(3-guanidinopropyl)-1,4-imino-D-ribitol
-
pH 7.0, 35°C
0.0061
1,4-dideoxy-1,4-imino-D-ribitol
-
pH 7.0, 35°C
0.00075
1,4-dideoxy-1,4-imino-N-(1-isoquinolinyl)methyl-D-ribitol
-
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.00072
1,4-dideoxy-1,4-imino-N-(1-methyl-1H-1,2,3-benzotriazol-5-yl)methyl-D-ribitol
-
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.0021
1,4-dideoxy-1,4-imino-N-(1-methyl-1H-benzimidazol-2-yl)methyl-D-ribitol
-
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.187
1,4-dideoxy-1,4-imino-N-(2(1H)-quinolon-8-yl)methyl-D-ribitol
-
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.00004
1,4-dideoxy-1,4-imino-N-(2-amino-4-benzothiazoyl)-methyl-D-ribitol
-
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.12
1,4-dideoxy-1,4-imino-N-(2-aminopyridin-3-yl)methyl-D-ribitol
-
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.00836
1,4-dideoxy-1,4-imino-N-(2-aminoquinolin-8-yl)methyl-D-ribitol
-
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.000314
1,4-dideoxy-1,4-imino-N-(2-chloroquinolin-8-yl)methyl-D-ribitol
-
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.001258
1,4-dideoxy-1,4-imino-N-(2-methoxyquinolin-8-yl)methyl-D-ribitol
-
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.002
1,4-dideoxy-1,4-imino-N-(2-naphthyl)methyl-D-ribitol
-
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.00018
1,4-dideoxy-1,4-imino-N-(2-quinolinyl)methyl-D-ribitol
-
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.0038
1,4-dideoxy-1,4-imino-N-(3-cyanobenzyl)-D-ribitol
-
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.012
1,4-dideoxy-1,4-imino-N-(3-indolyl)methyl-D-ribitol
-
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.0023
1,4-dideoxy-1,4-imino-N-(3-nitrobenzyl)-D-ribitol
-
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.02
1,4-dideoxy-1,4-imino-N-(3-quinolinyl)methyl-D-ribitol
-
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.0131
1,4-dideoxy-1,4-imino-N-(4(1H)-quinolon-8-yl)methyl-D-ribitol
-
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.0067
1,4-dideoxy-1,4-imino-N-(4-amidinobenzyl)-D-ribitol
-
pH 7.0, 35°C
0.0713
1,4-dideoxy-1,4-imino-N-(4-amino-2-methylquinolin-8-yl)methyl-D-ribitol
-
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.000015
1,4-dideoxy-1,4-imino-N-(4-aminoquinazolin-8-yl)methyl-D-ribitol
-
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.00005
1,4-dideoxy-1,4-imino-N-(4-aminoquinolin-8-yl)methyl-D-ribitol
-
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.000019
1,4-dideoxy-1,4-imino-N-(4-aminothieno[3,2-d]pyrimidin-7-yl)-methyl-D-ribitol
-
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.000369
1,4-dideoxy-1,4-imino-N-(4-chloro-2-methylquinolin-8-yl)methyl-D-ribitol
-
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.000014
1,4-dideoxy-1,4-imino-N-(4-chloroquinolin-8-yl)methyl-D-ribitol
-
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.026
1,4-dideoxy-1,4-imino-N-(4-cyanobenzyl)-D-ribitol
-
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.007
1,4-dideoxy-1,4-imino-N-(4-guanidinobenzyl)-D-ribitol
-
pH 7.0, 35°C
0.000029
1,4-dideoxy-1,4-imino-N-(4-methoxyquinolin-8-yl)methyl-D-ribitol
-
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.043
1,4-dideoxy-1,4-imino-N-(4-methyl-2-phenylpyrimidin-5-yl)methyl-D-ribitol
-
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.02
1,4-dideoxy-1,4-imino-N-(4-pyridinyl)methyl-D-ribitol
-
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.029
1,4-dideoxy-1,4-imino-N-(4-quinolinyl)methyl-D-ribitol
-
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.032
1,4-dideoxy-1,4-imino-N-(6-methoxypyridin-3-yl)methyl-D-ribitol
-
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.0023
1,4-dideoxy-1,4-imino-N-(7-methoxy-indol-3-yl)methyl-D-ribitol
-
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.00072
1,4-dideoxy-1,4-imino-N-(7-quinolinyl)methyl-D-ribitol
-
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.00002
1,4-dideoxy-1,4-imino-N-(7-thieno[3,2-d]pyrimidonyl)-methyl-D-ribitol
-
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.001
1,4-dideoxy-1,4-imino-N-(8-hydroxyquinolin-2-yl)methyl-D-ribitol
-
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.0000108
1,4-dideoxy-1,4-imino-N-(8-quinolinyl)methyl-D-ribitol
-
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.000058
1,4-dideoxy-1,4-imino-N-(quinazolin-8-yl)methyl-D-ribitol
-
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.000109
1,4-dideoxy-1,4-imino-N-(quinazolon-8-yl)methyl-D-ribitol
-
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.000139
1,4-dideoxy-1,4-imino-N-(quinoxalin-5-yl)-methyl-D-ribitol
-
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.000042
1,4-dideoxy-1,4-imino-N-(thieno[3,2-d]pyrimidin-7-yl)methyl-D-ribitol
-
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.0034
1,4-dideoxy-1,4-imino-N-[3-(1H-pyrrol-1-yl)benzyl]-D-ribitol
-
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.000063 - 0.0012
1,4-dideoxy-1,4-imino-N-[4-(1H-1,2,4-triazol-1-yl)benzyl]-D-ribitol
0.0012
1,4-dideoxy-1,4-imino-N-[4-(1H-pyrazol-1-yl)benzyl]-D-ribitol
-
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.001
1,4-dideoxy-1,4-imino-N-[4-(1H-pyrrol-1-yl)benzyl]-D-ribitol
-
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.545
1-guanidino-2,5-anhydro-1-deoxy-D-allitol
-
pH 7.0, 35°C
0.309
1-guanidino-3,6-anhydro-1,2-dideoxy-D-allo-heptitol
-
pH 7.0, 35°C
0.00000048 - 0.0000063
1-[(7-benzyloxymethyl-6-tert-butoxy-9-deazapurin-9-yl)methyl] azetidine-3-methanol
0.000000229 - 0.000000665
1-[(9-deazahypoxanthin-9-yl)methyl]azetidine-3,3dimethanol
0.018
1-[2-[(2S,3S,4R,5R)-3,4-dihydroxy-5-(hydroxymethyl)pyrrolidin-2-yl]ethyl]guanidine
-
-
0.04
1-[3-[(2R,3R,4S)-3,4-dihydroxy-2-(hydroxymethyl)pyrrolidin-1-yl]propyl]guanidine
-
-
0.182
2'-deoxyadenosine
-
at pH 7.0 and 35°C
0.18
3'-deoxyadenosine
-
at pH 7.0 and 35°C
0.0002
3-deazaadenosine
-
pH 7.2, 35°C, His-tagged enzyme form
0.000001 - 0.0000011
4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin H
0.3565
7-deazaadenosine
-
pH 7.2, 35°C, His-tagged enzyme form
0.0000044
7-[[(2R,3R,4S)-3,4-dihydroxy-2-(hydroxymethyl)pyrrolidin-1-yl]methyl]-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one
-
in 50 mM phosphate buffer, pH 7.0, at 35°C
1.2
8-aza-immucillin H
-
mutant R252A, pH 7.0, 35°C; wild-type, pH 7.0, 35°C
0.000015
8-aza-immucillin-H
-
at pH 7.0 and 35°C
0.16 - 1
Co2+
0.0009
Cu2+
-
noncompetitive, recombinant enzyme, pH 7.3, 37°C
0.00211 - 0.016
cytidine
8.74
D-ribose
-
pH 7.2, 37°C
0.0038
daidzein
-
pH 7.3, 37°C
0.011
formononetin
-
pH 7.3, 37°C
0.0000009 - 6.2
immucillin A
0.0000062 - 6.2
immucillin H
0.000009
immucillin-A
-
at pH 7.0 and 35°C
0.000044
immucillin-H
-
at pH 7.0 and 35°C
0.0000129 - 0.000016
meso-[(9-deazahypoxanthin-9-yl)methyl]azetidine-2,4-dimethanol hydrochloride
0.29 - 1
Mn2+
0.0000041 - 0.59
N-(9-deaza-adenin-9-yl)methyl-1,4-dideoxy-1,4-imino-D-ribitol
0.0000044
N-(9-deazahypoxanthin-9-yl)methyl-1,4-dideoxy-1,4-imino-D-ribitol
-
in 50 mM phosphate buffer, pH 7.0, at 35°C
0.059
N-benzyl-1,4-dideoxy-1,4-imino-D-ribitol
-
pH 7.0, 35°C
0.028 - 0.39
Ni2+
0.0000044 - 0.138
UAMC-00109
0.000011 - 0.011
UAMC-00115
0.00004
UAMC-00218
-
pH and temperature not specified in the publication
0.00004 - 0.014
UAMC-00311
0.00002 - 0.12
UAMC-00312
0.000058
UAMC-00346
-
pH and temperature not specified in the publication
0.0000041 - 0.032
UAMC-00363
0.000019 - 0.031
UAMC-00375
0.0028 - 0.0167
Zn2+
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.034
UAMC-00312
Trypanosoma brucei brucei
-
pH and temperature not specified in the publication
0.00049
UAMC-00363
Trypanosoma brucei brucei
-
pH and temperature not specified in the publication
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
1.32
in 50 mM HEPES, pH 7.3, with guanosine as substrate
11.3
-
pH 7.2, 37°C
11.76
in 50 mM HEPES, pH 7.3, with adenosine as substrate
50
-
hydrolysis of inosine
57.2
in 50 mM HEPES, pH 7.3, with inosine as substrate
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3.5
-
hydrolysis of 3'-AMP
4 - 4.5
-
degradation of NAD+
4.5
E15229
hydrolysis of guanosine
5.5
E15229
hydrolysis of inosine
6.6
-
substrate xanthosine
7 - 8
-
-
7.3
-
assay at
7.5 - 8.5
-
hydrolysis of inosine and xanthosine
7.5
assay at
7.8
-
substrate inosine
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
1.5 - 5.3
-
about 50% of maximal activity at pH 1.5 and at pH 5.3
3.5 - 9
E15229
hydrolysis of purine nucleosides
5 - 8
-
pH 5.0: about 55% of maximal activity, pH 8.0: about 40% of maximal activity
5 - 10
inosine hydrolase activity of the purified recombinant IunH is detected over a wide range of pH values, the highest activity being obtained at pH 6
9
-
substrate inosine, more than 50% of maximum activity
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30 - 70
-
30°C: about 35% of maximal activity, 70°C: about 20% of maximal activity
40 - 70
-
40°C: about 55% of maximal activity with inosine, about 45% of maximal activity with guanosine, about 40% of maximal activity with adenosine, 70°C: about 30% of maximal activity with inosine, about 35% of maximal activity with guanosine, about 25% of maximal activity with adenosine
40 - 100
the activity is stable over a wide temperature range, the highest activity being at 80°C with high activity being maintained up to 100°C
60 - 115
-
the enzyme is highly thermoactive, its activity increases sharply up to the optimal temperature of 105°C and 50% activity is still observed at 115°C
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.9
-
isoelectrofocusing
5.9
-
estimated isoelectric point
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
secreted, activity is much larger in female salivary glands than in male
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
PDB
SCOP
CATH
ORGANISM
UNIPROT
Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Trypanosoma brucei brucei (strain 927/4 GUTat10.1)
Trypanosoma brucei brucei (strain 927/4 GUTat10.1)
Trypanosoma brucei brucei (strain 927/4 GUTat10.1)
Trypanosoma brucei brucei (strain 927/4 GUTat10.1)
Trypanosoma brucei brucei (strain 927/4 GUTat10.1)
Trypanosoma brucei brucei (strain 927/4 GUTat10.1)
Trypanosoma brucei brucei (strain 927/4 GUTat10.1)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30600
-
5 * 30600, SDS-PAGE
32310
-
x * 35892, isoform rih1, calculated, x * 32310, isoform rih2, calculated
33100
-
4 * 33100, calculated
34000
-
4 * 34000, SDS-PAGE
34325
-
2 * 34325, calculated from sequence of cDNA
35000
-
subunit, SDS-PAGE
35600
-
x * 35600, SDS-PAGE
35759
-
2 * 35759, electrospray ionization mass spectrometry
35892
-
x * 35892, isoform rih1, calculated, x * 32310, isoform rih2, calculated
36200
calculated from amino acid sequence
38000
-
4 * 38000
39000
-
4 * 39000
41000
-
2 * 41000, SDS-PAGE
51500
-
gel filtration
68650
-
calculated from sequence of cDNA
71000
-
gel filtration
128000
-
sucrose density gradient centrifugation
142000
-
gel filtration
143000
-
gel filtration
160000
-
gel filtration
172000
E15229
gel filtration
205000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
-
2 * 35759, electrospray ionization mass spectrometry; 2 * 41000, SDS-PAGE
homodimer
homotetramer
-
x-ray crystallography
pentamer
-
5 * 30600, SDS-PAGE
tetramer