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Literature summary for 3.2.2.1 extracted from

  • Ogawa, J.; Takeda, S.; Xie, S.X.; Hatanaka, H.; Ashikari, T.; Amachi, T.; Shimizu, S.
    Purification, characterization, and gene cloning of purine nucleosidase from Ochrobactrum anthropi (2001), Appl. Environ. Microbiol., 67, 1783-1787.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.2.1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Brucella anthropi

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 1 mM, 23% inhibition Brucella anthropi
8-hydroxyquinoline 1 mM, 93% inhibition Brucella anthropi
Ag+ 1 mM, 68% inhibition Brucella anthropi
Cu2+ 1 mM, 57% inhibition Brucella anthropi
cytidine 0.5 mM, 81% inhibition of activity with adenosine, 92% inhibition of activity with inosine, 91% inhibition of the activity with xanthosine, 94% inhibition of activity with guanosine Brucella anthropi
EDTA 1 mM, 46% inhibition Brucella anthropi
Hg2+ 1 mM,61% inhibition Brucella anthropi
Mn2+ 1 mM, 34% inhibition Brucella anthropi
NaAsO2
-
 Brucella anthropi
NaCN
-
 Brucella anthropi
NaF
-
 Brucella anthropi
NaN3
-
 Brucella anthropi
uridine 0.5 mM, 38% inhibition of activity with adenosine, 45% inhibition of activity with inosine, 35% inhibition of the activity with xanthosine, 58% inhibition of activity with guanosine Brucella anthropi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0118
-
 adenosine
-
 Brucella anthropi
0.0158
-
 Xanthosine
-
 Brucella anthropi
0.0395
-
 Inosine
-
 Brucella anthropi
0.0563
-
 guanosine
-
 Brucella anthropi

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 1 mM, slightly enhances activity to 120% Brucella anthropi
Co2+ 3 mM, reactivation after preincubation with 8-hydroxyquinoline Brucella anthropi
Zn2+ 3 mM, reactivation after preincubation with 8-hydroxyquinoline Brucella anthropi

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 4 * 40000, SDS-PAGE Brucella anthropi
172000
-
 gel filtration Brucella anthropi

Organism

Organism UniProt Comment Textmining
Brucella anthropi E15229
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Brucella anthropi

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
90.3
-
-
 Brucella anthropi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-AMP + H2O 0.67% of the activity with adenosine Brucella anthropi adenine + D-ribose 3-phosphate
-
 ?
adenosine + H2O
-
 Brucella anthropi adenine + D-ribose
-
 ?
guanosine + H2O 29.5% of the activity with adenosine Brucella anthropi guanine + D-ribose
-
 ?
inosine + H2O 78.9% of the activity with adenosine Brucella anthropi hypoxanthine + D-ribose
-
 ?
xanthosine + H2O 63.5% of the activity with adenosine Brucella anthropi xanthine + D-ribose
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 40000, SDS-PAGE Brucella anthropi

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Brucella anthropi

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 pH 6.0, 30 min, stable up to Brucella anthropi
50
-
 pH 6.0, 30 min, 50% loss of activity Brucella anthropi
60
-
 pH 6.0, 30 min, 80% loss of activity Brucella anthropi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
 hydrolysis of guanosine Brucella anthropi
5.5
-
 hydrolysis of inosine Brucella anthropi
6.5
-
 hydrolysis of adenosine Brucella anthropi

pH Range

pH Minimum pH Maximum Comment Organism
3.5 9 hydrolysis of purine nucleosides Brucella anthropi

pH Stability

pH Stability pH Stability Maximum Comment Organism
4
-
 30°C, 30 min, retains 40% of the initial activity Brucella anthropi
6 8.5 30°C, 30 min, enzyme retains more than 80% of the initial activity Brucella anthropi
9
-
 30°C, 30 min, retains 40% of the initial activity Brucella anthropi

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00211
-
 cytidine pH 7.0, 30°C Brucella anthropi
0.00415
-
 cytidine pH 7.0, 30°C Brucella anthropi
0.0112
-
 cytidine pH 7.0, 30°C Brucella anthropi
0.016
-
 cytidine pH 7.0, 30°C Brucella anthropi