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Literature summary for 3.2.2.1 extracted from

  • Versees, W.; Barlow, J.; Steyaert, J.
    Transition-state complex of the purine-specific nucleoside hydrolase of T. vivax: enzyme conformational changes and implications for catalysis (2006), J. Mol. Biol., 359, 331-346.
    View publication on PubMed
Search for more literature for 3.2.2.1

Crystallization (Commentary)

Crystallization (Comment) Organism
both soaked and co-crystallized with transition-state inhibitor immucillin H Trypanosoma vivax

Inhibitors

Inhibitors Comment Organism Structure
8-aza-immucillin A
-
 Trypanosoma vivax
8-aza-immucillin H
-
 Trypanosoma vivax
immucillin A
-
 Trypanosoma vivax
immucillin H transition-state inhibitor, i.e. (1S)-1-(9-deazahypoxanthin-9-yl)-1,4-dideoxy-1,4-imino-D-ribitol Trypanosoma vivax

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0021
-
 7-methyl guanosine wild-type, pH 7.0, 35°C Trypanosoma vivax
0.0023
-
 guanosine wild-type, pH 7.0, 35°C Trypanosoma vivax
0.0024
-
 7-methyl guanosine mutant R252A, pH 7.0, 35°C Trypanosoma vivax
0.004
-
 purine ribonucleoside wild-type, pH 7.0, 35°C Trypanosoma vivax
0.0066
-
 adenosine mutant R252A, pH 7.0, 35°C Trypanosoma vivax
0.008
-
 7-methyl guanosine mutant D40A, pH 7.0, 35°C Trypanosoma vivax
0.008
-
 adenosine wild-type, pH 7.0, 35°C Trypanosoma vivax
0.011
-
 purine ribonucleoside mutant R252A, pH 7.0, 35°C Trypanosoma vivax
0.031
-
 guanosine mutant R252A, pH 7.0, 35°C Trypanosoma vivax
0.066
-
 guanosine mutant D40A, pH 7.0, 35°C Trypanosoma vivax
0.925
-
 cytidine wild-type, pH 7.0, 35°C Trypanosoma vivax
1.06
-
 cytidine mutant R252A, pH 7.0, 35°C Trypanosoma vivax

Organism

Organism UniProt Comment Textmining
Trypanosoma vivax
-
-
-

Reaction

Reaction Comment Organism Reaction ID
a purine nucleoside + H2O = D-ribose + a purine base mechanism couples loop closure to the positioning of active site residues. Relationship between the dimeric form of the enzyme and its catalytic activity Trypanosoma vivax
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7-methyl guanosine + H2O
-
 Trypanosoma vivax D-ribose + 7-methyl guanine
-
 ?
adenosine + H2O
-
 Trypanosoma vivax D-ribose + adenine
-
 ?
cytidine + H2O
-
 Trypanosoma vivax cytosine + D-ribose
-
 ?
guanosine + H2O
-
 Trypanosoma vivax D-ribose + guanine
-
 ?
purine ribonucleoside + H2O
-
 Trypanosoma vivax purine + D-ribose
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.338
-
 cytidine wild-type, pH 7.0, 35°C Trypanosoma vivax
0.54
-
 cytidine mutant R252A, pH 7.0, 35°C Trypanosoma vivax
2
-
 purine ribonucleoside mutant R252A, pH 7.0, 35°C Trypanosoma vivax
2.3
-
 guanosine wild-type, pH 7.0, 35°C Trypanosoma vivax
2.6
-
 adenosine wild-type, pH 7.0, 35°C Trypanosoma vivax
2.7
-
 guanosine mutant D40A, pH 7.0, 35°C Trypanosoma vivax
3.4
-
 7-methyl guanosine wild-type, pH 7.0, 35°C Trypanosoma vivax
3.9
-
 purine ribonucleoside wild-type, pH 7.0, 35°C Trypanosoma vivax
5.7
-
 adenosine mutant R252A, pH 7.0, 35°C Trypanosoma vivax
9.8
-
 guanosine mutant R252A, pH 7.0, 35°C Trypanosoma vivax
13.2
-
 7-methyl guanosine mutant R252A, pH 7.0, 35°C Trypanosoma vivax
13.6
-
 7-methyl guanosine mutant D40A, pH 7.0, 35°C Trypanosoma vivax

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.2
-
 8-aza-immucillin H mutant R252A, pH 7.0, 35°C Trypanosoma vivax
1.2
-
 8-aza-immucillin H wild-type, pH 7.0, 35°C Trypanosoma vivax
6.2
-
 immucillin H mutant R252A, pH 7.0, 35°C Trypanosoma vivax
6.2
-
 immucillin A mutant R252A, pH 7.0, 35°C Trypanosoma vivax
6.2
-
 immucillin H wild-type, pH 7.0, 35°C Trypanosoma vivax
6.2
-
 immucillin A wild-type, pH 7.0, 35°C Trypanosoma vivax