Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | increases activity | Aspergillus niger | |
GSH | increases activity | Aspergillus niger |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 0.01 M, 28% inhibition. 0.02 M 42% inhibition | Aspergillus niger | |
HgCl2 | - |
Aspergillus niger | |
iodoacetate | 0.01 M, 25% inhibition. 0.02 M 36.1% inhibition | Aspergillus niger |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.1 | - |
Inosine | - |
Aspergillus niger | |
7.5 | - |
guanosine | - |
Aspergillus niger | |
9 | - |
adenosine | - |
Aspergillus niger |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | increases activity | Aspergillus niger |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Aspergillus niger |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4 | - |
- |
Aspergillus niger |
Storage Stability | Organism |
---|---|
4°C, 0.1 M citrate buffer, pH 4.0, stable for more than 3 months | Aspergillus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
adenosine + H2O | - |
Aspergillus niger | D-ribose + adenine | - |
? | |
guanosine + H2O | - |
Aspergillus niger | D-ribose + guanine | - |
? | |
inosine + H2O | - |
Aspergillus niger | hypoxanthine + D-ribose | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Aspergillus niger |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 70 | 30°C: about 35% of maximal activity, 70°C: about 20% of maximal activity | Aspergillus niger |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
60 min, complete loss of activity. 30 min, 30% loss of activity | Aspergillus niger |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3 | 4 | - |
Aspergillus niger |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
1.5 | 5.3 | about 50% of maximal activity at pH 1.5 and at pH 5.3 | Aspergillus niger |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8 | - |
unstale | Aspergillus niger |