Information on EC 3.2.1.85 - 6-phospho-beta-galactosidase

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The expected taxonomic range for this enzyme is: Bacteria

EC NUMBER
COMMENTARY
3.2.1.85
-
RECOMMENDED NAME
GeneOntology No.
6-phospho-beta-galactosidase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
a 6-phospho-beta-D-galactoside + H2O = 6-phospho-D-galactose + an alcohol
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
hydrolysis of O-glycosyl bond
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
Galactose metabolism
-
lactose and galactose degradation I
-
Metabolic pathways
-
SYSTEMATIC NAME
IUBMB Comments
6-phospho-beta-D-galactoside 6-phosphogalactohydrolase
-
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6-phospho-beta-D-galactosidase
-
-
beta-D-phosphogalactosidase
-
-
beta-D-phosphogalactoside galactohydrolase
-
-
beta-D-phosphogalactoside galactohydrolase
-
-
beta-D-phosphogalactoside galactohydrolase
-
-
-
beta-Pgal
-
-
beta-phosphogalactosidase
-
-
BGase-III
-
-
BGase-III
Klebsiella sp. RE 1755A
-
-
-
P-beta-gal
-
I and II
P-beta-gal
Lactobacillus gasseri JCM 1031
-
I and II
-
P-beta-galactosidase
-
-
P-bgal
Lactobacillus acidophilus JCM1132T
-
-
-
P-bgal
Lactobacillus johnsonii JCM2122
-
-
-
PBG
-
-
-
-
phospho-beta-galactosidase
-
-
phospho-beta-galactosidase
-
-
phospho-beta-galactosidase
Klebsiella sp. RE 1755A
-
-
-
phospho-beta-galactosidase
-
-
phospho-beta-galactosidase
Lactobacillus acidophilus JCM1132T
-
-
-
phospho-beta-galactosidase
Lactobacillus johnsonii JCM2122
-
-
-
phospho-beta-galactosidase
-
-
phospho-beta-galactosidase
-
-
phospho-beta-galactosidase
-
-
phospho-beta-galactosidase
-
-
phospho-beta-galactosidase
-
-
CAS REGISTRY NUMBER
COMMENTARY
37237-42-6
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
strain ATCC 824
-
-
Manually annotated by BRENDA team
strain RE 1755A, Lac+Gal+ mutant CT-1
-
-
Manually annotated by BRENDA team
Klebsiella sp. RE 1755A
strain RE 1755A, Lac+Gal+ mutant CT-1
-
-
Manually annotated by BRENDA team
strain JCM1132T and strain LA117
-
-
Manually annotated by BRENDA team
strain MEP181R45, strain MEP181R50 and strain MEP181R51
-
-
Manually annotated by BRENDA team
L. casei 9595
-
-
Manually annotated by BRENDA team
strain JCM1134T, strain MEP181R52, strain MEP181R53, strain MEP181R54 and strain NIAI C-9
-
-
Manually annotated by BRENDA team
subsp. casei 64H
-
-
Manually annotated by BRENDA team
strain MEP181R55 (very low actvity), strain MEP181R56 (low activity)
-
-
Manually annotated by BRENDA team
strain MEP181R57 (very low activity) and strain MEP181R58 (very low activity)
-
-
Manually annotated by BRENDA team
strain ATCC33323T, strain JCM1031, strain OLL2836 (high activity), strain OLL2948, strain MEP181R34 (high activity), strain MEP181R35, strain MEP181R36, strain MEP181R40 (strain activity), strain MEP181R41, strain MEP181R42 (low activity), strain MEP181R43, strain MEP181R44 (low activity), strain MEP181R46 (high activity), strain MEP181R47 (low activity), strain MEP181R48, strain MEP181R49 (high activity), strain MEP181R65
-
-
Manually annotated by BRENDA team
Lactobacillus gasseri JCM 1031
JCM 1031
-
-
Manually annotated by BRENDA team
strain JCM2122 (very low activity)
-
-
Manually annotated by BRENDA team
Lactobacillus johnsonii JCM2122
strain JCM2122 (very low activity)
-
-
Manually annotated by BRENDA team
strain OLL2848 (very low activity), strain MEP181R68 (very low activity), strain MEP181R69 (very low activity), strain MEP181R70 (very low activity), strain MEP181R71 (very low activity), strainj MEP181R72 (very low activity), strain MEP181R74 (low activity)
-
-
Manually annotated by BRENDA team
subsp. lactis, wild-type strains and enzyme-deficient mutant strain IL1403, overview
-
-
Manually annotated by BRENDA team
strain ATCC 14201
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-nitrophenyl beta-D-galactopyranoside 6-phosphate + H2O
2-nitrophenyl beta-D-galactopyranose + phosphate
show the reaction diagram
-
-
-
-
?
4-nitrophenyl 6-phospho-beta-D-galactopyranoside + H2O
4-nitrophenol + 6-phospho-beta-D-galactopyranose
show the reaction diagram
-
-
-
-
?
4-nitrophenyl 6-phospho-beta-D-glucopyranoside + H2O
4-nitrophenol + 6-phospho-beta-D-glucopyranose
show the reaction diagram
-
-
-
-
?
lactose + H2O
D-glucose + D-galactose
show the reaction diagram
-
-, existence of an alternative lactose catabolic pathway in the enzyme-deficient mutant strain IL1403 by phospho-beta-galactosidase activity exhibited by BglS, a phospho-beta-glucosidase, the lactose hydrolysis is catalyzed by both phospho-beta-galactosidase and phospho-beta-glucosidase, the latter being the main enzyme in the pathway
-
-
?
lactose 6-phosphate + H2O
D-glucose + D-galactose 6-phosphate
show the reaction diagram
-
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
show the reaction diagram
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
show the reaction diagram
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
show the reaction diagram
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
show the reaction diagram
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
show the reaction diagram
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
show the reaction diagram
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
show the reaction diagram
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
show the reaction diagram
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
show the reaction diagram
P11546
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
show the reaction diagram
-
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
show the reaction diagram
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
show the reaction diagram
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
show the reaction diagram
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
show the reaction diagram
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
show the reaction diagram
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
show the reaction diagram
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
show the reaction diagram
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
show the reaction diagram
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
show the reaction diagram
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
show the reaction diagram
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
show the reaction diagram
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
show the reaction diagram
-
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
show the reaction diagram
-
involved in lactose assimilation
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
show the reaction diagram
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
show the reaction diagram
Lactobacillus gasseri JCM 1031
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
show the reaction diagram
Lactobacillus gasseri JCM 1031
-
-
-
?
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
o-nitrophenol + beta-D-galactose-6-phosphate
show the reaction diagram
-
-
-
?
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
o-nitrophenol + beta-D-galactose-6-phosphate
show the reaction diagram
-
-
-
?
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
o-nitrophenol + beta-D-galactose-6-phosphate
show the reaction diagram
-
-
-
?
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
o-nitrophenol + beta-D-galactose-6-phosphate
show the reaction diagram
-
-
-
?
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
o-nitrophenol + beta-D-galactose-6-phosphate
show the reaction diagram
-
-
-
?
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
o-nitrophenol + beta-D-galactose-6-phosphate
show the reaction diagram
-
-
-
?
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
o-nitrophenol + beta-D-galactose-6-phosphate
show the reaction diagram
-
-
-
?
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
o-nitrophenol + beta-D-galactose-6-phosphate
show the reaction diagram
-
-
-
?
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
o-nitrophenol + beta-D-galactose-6-phosphate
show the reaction diagram
-
-
-
-
?
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
o-nitrophenol + beta-D-galactose-6-phosphate
show the reaction diagram
-
-
-
?
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
o-nitrophenol + beta-D-galactose-6-phosphate
show the reaction diagram
-
-
-
?
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
o-nitrophenol + beta-D-galactose-6-phosphate
show the reaction diagram
-
-
-
?
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
o-nitrophenol + beta-D-galactose-6-phosphate
show the reaction diagram
-
-
-
?
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
o-nitrophenol + beta-D-galactose-6-phosphate
show the reaction diagram
-
-
-
?
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
o-nitrophenol + beta-D-galactose-6-phosphate
show the reaction diagram
-
-
-
?
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
o-nitrophenol + beta-D-galactose-6-phosphate
show the reaction diagram
-
-
-
?
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
o-nitrophenol + beta-D-galactose-6-phosphate
show the reaction diagram
-
-
-
?
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
o-nitrophenol + beta-D-galactose-6-phosphate
show the reaction diagram
-
-
-
?
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
o-nitrophenol + beta-D-galactose-6-phosphate
show the reaction diagram
-
-
-
-
?
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
o-nitrophenol + beta-D-galactose-6-phosphate
show the reaction diagram
-
-
-
?
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
o-nitrophenol + beta-D-galactose-6-phosphate
show the reaction diagram
Lactobacillus gasseri JCM 1031
-
-
-
?
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
o-nitrophenol + beta-D-galactose-6-phosphate
show the reaction diagram
Lactobacillus gasseri JCM 1031
-
-
-
?
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
o-nitrophenol + beta-D-galactose-6-phosphate
show the reaction diagram
Klebsiella sp. RE 1755A
-
-
-
?
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
o-nitrophenol + beta-D-galactose 6-phosphate
show the reaction diagram
-
-
-
-
?
o-nitrophenyl-beta-D-galactoside + H2O
o-nitrophenol + beta-D-galactose
show the reaction diagram
-
-
-
-
?
o-nitrophenyl-beta-D-glucopyranoside 6-phosphate
o-nitrophenol + beta-D-glucose-6-phosphate
show the reaction diagram
-
only little activity
-
?
o-nitrophenyl-beta-D-glucopyranoside 6-phosphate + H2O
o-nitrophenol + beta-D-glucose-6-phosphate
show the reaction diagram
-
-
-
-
?
p-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
p-nitrophenol + beta-D-galactose-6-phosphate
show the reaction diagram
-
-
-
?
p-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
p-nitrophenol + beta-D-galactose-6-phosphate
show the reaction diagram
-
-
-
?
p-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
p-nitrophenol + beta-D-galactose-6-phosphate
show the reaction diagram
-
-
-
?
p-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
p-nitrophenol + beta-D-galactose-6-phosphate
show the reaction diagram
-
less effective than ortho-form
-
-
?
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate
p-nitrophenol + beta-D-glucose-6-phosphate
show the reaction diagram
-
only little activity
-
?
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate + H2O
p-nitrophenol + beta-D-glucose-6-phosphate
show the reaction diagram
-
less effective than ortho-form
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
show the reaction diagram
Klebsiella sp. RE 1755A
-
-
-
?
additional information
?
-
-
physiologic regulation of the pathway, overview
-
-
-
additional information
?
-
-
Pyrococcus furiosus beta-glucosidase has been mutated to increase its activity toward galactosides by incorporating residues present in Lactococcus lactis 6-phospho-beta-galactosidase
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
lactose + H2O
D-glucose + D-galactose
show the reaction diagram
-
existence of an alternative lactose catabolic pathway in the enzyme-deficient mutant strain IL1403 by phospho-beta-galactosidase activity exhibited by BglS, a phospho-beta-glucosidase, the lactose hydrolysis is catalyzed by both phospho-beta-galactosidase and phospho-beta-glucosidase, the latter being the main enzyme in the pathway
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
show the reaction diagram
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
show the reaction diagram
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
show the reaction diagram
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
show the reaction diagram
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
show the reaction diagram
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
show the reaction diagram
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
show the reaction diagram
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
show the reaction diagram
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
show the reaction diagram
P11546
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
show the reaction diagram
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
show the reaction diagram
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
show the reaction diagram
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
show the reaction diagram
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
show the reaction diagram
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
show the reaction diagram
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
show the reaction diagram
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
show the reaction diagram
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
show the reaction diagram
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
show the reaction diagram
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
show the reaction diagram
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
show the reaction diagram
-
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
show the reaction diagram
-
involved in lactose assimilation
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
show the reaction diagram
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
show the reaction diagram
Lactobacillus gasseri JCM 1031
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
show the reaction diagram
Lactobacillus gasseri JCM 1031
-
-
-
?
lactose-6-phosphate + H2O
D-glucose + beta-D-galactose-6-phosphate
show the reaction diagram
Klebsiella sp. RE 1755A
-
-
-
?
additional information
?
-
-
physiologic regulation of the pathway, overview
-
-
-
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Ca2+
-
78% of activity at 1 mM CaCl2
Co2+
-
14% inhibition at 5 mM
Cu2+
-
100% inhibition at 5 mM
Hg2+
-
complete inhibition at 1 mM HgCl2
Li+
-
82% of control activity at 2 mM
Mg2+
-
74.5% of control activity at 2 mM
Mg2+
-
slight inhibition at 1 mM MgCl2
Mn2+
-
50% inactivation to 10 mM
Zn2+
-
57% inhibition at 5 mM
Zn2+
-
98.4% inhibition at 1 mM ZnSO4
Mn2+
-
slight inhibition at 1 mM MnCl2
additional information
-
not required
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
2,4-dinitrophenyl-2-deoxy-2-bromo-beta-D-galactopyranosyl-6-phosphate
-
fluoro-, chloro- and bromo-derivatives, complete inactivation, most rapidly with fluoro-, followed by chloro- and bromo-derivatives
-
2,4-dinitrophenyl-2-deoxy-2-chloro-beta-D-galactopyranosyl-6-phosphate
-
fluoro-, chloro- and bromo-derivatives, complete inactivation, most rapidly with fluoro-, followed by chloro- and bromo-derivatives
-
2,4-dinitrophenyl-2-deoxy-2-fluoro-beta-D-galactopyranosyl-6-phosphate
-
fluoro-, chloro- and bromo-derivatives, complete inactivation, most rapidly with fluoro-, followed by chloro- and bromo-derivatives
-
2-phosphoglycerate
-
26% inhibition at 4.2 mM
ATP
-
100% inhibition at 2.1 mM
D-fructose-1,6-diphosphate
-
19% inhibition at 4.2 mM
D-fructose-6-phosphate
-
20% inhibition at 4.2 mM
D-galactose-6-phosphate
-
-
D-galactose-6-phosphate
-
55% inhibition at 4.2 mM
D-galactose-6-phosphate
-
competitive inhibition
D-galactose-6-phosphate
-
-
D-galactose-6-phosphate
-
competitive inhibition; weak inhibition
D-Glucose-6-phosphate
-
38% inhibition at 4.2 mM
D-Glucose-6-phosphate
-
63% of activity
galactose-6-phosphate
-
-
p-chloromercuribenzoic acid
-
-
p-hydroxymercuribenzoate
-
83.4% of control activity at 1 mM, 12.2% at 2 mM
p-nitrophenyl-beta-thiogalactoside 6-phosphate
-
competitive inhibition
p-nitrophenyl-beta-thioglucoside 6-phosphate
-
competitive inhibition
phosphoenolpyruvate
-
97% inhibition at 1 mM
phosphoenolpyruvate
-
weak inhibition
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
2-mercaptoethanol
-
-
2-mercaptoethanol
-
lower effect on P-beta-gal II than P-beta-gal I
dithiothreitol
-
enhanced activiy after dialysis by addition up to 0.75 mM
EDTA
-
slight stimulation to 118.2% by 2 mM
EDTA
-
30% increase of activity
Fe2+
-
60% increase of activity
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.19
-
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate
-
-
0.4
-
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate
-
-
0.54
-
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate
-
pH 7.5, 37C
0.54
-
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate
-
wild type enzyme, pH 7.5, room temperature
0.59
-
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate
-
-
0.6
3
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate
-
-
0.6
3
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate
-
2.0 hybrid with protein A
0.6
3
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate
-
-
0.76
0.8
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate
-
-
1.3
1.59
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate
-
-
2.86
-
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate
-
W429A mutant enzyme, pH 7.5, room temperature
3
-
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate
-
with strain CT-1
4.89
-
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate
-
K435V/Y437F mutant enzyme, pH 7.5, room temperature
7.3
-
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate
-
with strain RE1755A
0.32
-
o-nitrophenyl-beta-D-galactoside
-
wild type enzyme, pH 7.5, room temperature
0.57
-
o-nitrophenyl-beta-D-galactoside
-
K435V/Y437F mutant enzyme, pH 7.5, room temperature
0.04
-
o-nitrophenyl-beta-D-glucopyranoside 6-phosphate
-
-
0.15
-
o-nitrophenyl-beta-D-glucopyranoside 6-phosphate
-
-
0.16
-
o-nitrophenyl-beta-D-glucopyranoside 6-phosphate
-
-
1.56
-
o-nitrophenyl-beta-D-glucopyranoside 6-phosphate
-
wild type enzyme, pH 7.5, room temperature
9.83
-
o-nitrophenyl-beta-D-glucopyranoside 6-phosphate
-
W429A mutant enzyme, pH 7.5, room temperature
0.09
-
p-nitrophenyl-6-phospho-beta-D-galactopyranose
-
-
0.1
-
p-nitrophenyl-6-phospho-beta-D-glucopyranose
-
-
0.09
-
p-nitrophenyl-beta-D-galactopyranoside 6-phosphate
-
-
0.2
-
p-nitrophenyl-beta-D-galactopyranoside 6-phosphate
-
-
0.3
-
p-nitrophenyl-beta-D-galactopyranoside 6-phosphate
-
-
0.39
-
p-nitrophenyl-beta-D-galactopyranoside 6-phosphate
-
wild type enzyme, pH 7.5, room temperature
3.1
-
p-nitrophenyl-beta-D-galactopyranoside 6-phosphate
-
W429A mutant enzyme, pH 7.5, room temperature
0.4
-
p-nitrophenyl-beta-D-glucopyranoside 6 phosphate
-
-
0.1
-
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate
-
-
0.6
-
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate
-
-
2.99
-
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate
-
wild type enzyme, pH 7.5, room temperature
21.53
-
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate
-
W429A mutant enzyme, pH 7.5, room temperature
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
57
-
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate
-
-
63
-
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate
-
-
83
-
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate
-
-
4
-
o-nitrophenyl-beta-D-glucopyranoside 6-phosphate
-
-
7
-
o-nitrophenyl-beta-D-glucopyranoside 6-phosphate
-
-
9
-
o-nitrophenyl-beta-D-glucopyranoside 6-phosphate
-
-
22
-
p-nitrophenyl-6-phospho-beta-D-galactopyranose
-
-
5
-
p-nitrophenyl-6-phospho-beta-D-glucopyranose
-
-
7
-
p-nitrophenyl-beta-D-galactopyranoside 6-phosphate
-
-
18
-
p-nitrophenyl-beta-D-galactopyranoside 6-phosphate
-
-
22
-
p-nitrophenyl-beta-D-galactopyranoside 6-phosphate
-
-
2
-
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate
-
-
4
-
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate
-
-
5
-
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.004
-
-
with citrate-grown cell-extracts of CT-1
0.032
-
-
with lactose-grown cell-extracts of CT-1
0.28
0.37
-
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate as substrate
0.28
-
-
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate as substrate, maximum of activity with Streptococcus lactis strain MG1299 after growth on glucose
1.45
-
-
p-nitrophenyl-beta-D-galactopyranoside 6-phosphate as substrate, 35 mM phosphate buffer, pH 6.8
2.44
-
-
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate as substrate, maximum of activity with Streptococcus lactis strain MG1299 after growth on lactose
20.9
-
-
purified enzyme
21.6
-
-
purified enzyme with o-nitrophenyl-beta-D-galactopyranoside 6-phosphate as substrate, pH 7.5, 37C
23.8
-
-
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate as substrate
28.9
-
-
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate as substrate, maximum of activity
100
-
-
maximal activity of hybrid with protein A
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6.5
-
-
most active in Tris-HCl buffer
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5
7
-
at 37C for 15 min
5
8
-
nearly constant activity
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
30
-
-
P-beta-gal II
30
-
-
assay at
37
-
-
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate
45
52
-
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate as substrate
50
-
-
P-beta-gal I
pI VALUE
pI VALUE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
4.9
-
-
isoelectric focusing
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
-
activity is induced during growth on lactose but is absent in glucose-grown cells
Manually annotated by BRENDA team
additional information
-
growth condition requirements of mutant strains
Manually annotated by BRENDA team
additional information
-
the enzyme occurs in bacteria grown on diary and milk, but lack in fish isolates, overview
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
35000
56000
-
gel filtration, SDS-PAGE
40000
-
-
strain SL1, gel filtration
46000
53000
-
-
46000
53000
-
gel filtration, SDS-PAGE
53000
-
-
SDS-PAGE
53990
-
-
DNA sequence analysis
55100
56500
-
DNA sequence analysis, SDS-PAGE
58000
-
-
SDS-PAGE
67600
-
-
gel filtration
130000
-
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
monomer
-
1 * 50000, SDS-PAGE
Crystallization/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
hanging drop method
-
pH STABILITY
pH STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
4
9
-
at 37C for 15 min
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
40
60
-
P-beta-gal II, stable below 40C, inactivated at 60C
50
-
-
rapid thermal inactivation, about 50% of activity after 3 min with extracts of strain CT-1, after 5 min with RE1755A
55
-
-
50% of activity after 5 min in Tris-buffer
70
-
-
stable up to for 5 min
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-70C, purified enzyme, at least 6 months
-
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
expressed in Escherichia coli
-
DNA and amino acid sequence determination and analysis, the enzyme is encoded in the galoperon, including a galactose permease and the Leloir pathway enzymes
-
expressed in Escherichia coli
-
expressed in Escherichia coli K12 TG1
-
subcloning in Escherichia coli, expression of wild-type and mutant enzymes in Lactococcus lactis strain IBB50
-
expressed in Escherichia coli
-
EXPRESSION
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
the enzyme is induced by growth on lactose, as is the whole lactose catabolic system in Lactococcus garvieae, overview
-
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
S428D/K435V/Y437F
-
no activity
W429A
-
improved specificity for glucosides
E160Q
-
1000fold reduction in activity compared to wild type
E375Q
-
complete loss of activity
K435V/Y437F
-
significantly reduced substrate binding and turnover
additional information
-
construction of a disruption mutant of gene bglS, encoding phospho-beta-glucosidase, in phospho-beta-galactosidase-deficient strain IL1403, genetic organization and chromosomal localizations in mutant strain IL1403 showig the beta-galactosidase-negative phenotype
G332R
-
complete loss of catalytic activity, but same behavior during purification and in affinity to antisera as wild type
additional information
-
hybrid protein with protein A
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
diagnostics
-
phospho-beta-galactosidase is a reliable genetic marker for biotypes of Lactococcus garvieae