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Literature summary for 3.2.1.85 extracted from

  • Witt, E.; Stewart, G.C.; Hengstenberg, W.
    A new protein that replaces the use of secondary antibodies engineered from the two staphylococcal enzymes protein A and 6-phospho-beta-galactosidase (1992), Protein Eng., 5, 267-271.
    View publication on PubMed
Search for more literature for 3.2.1.85

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Staphylococcus aureus

Protein Variants

Protein Variants Comment Organism
additional information hybrid protein with protein A Staphylococcus aureus

Inhibitors

Inhibitors Comment Organism Structure
D-galactose-6-phosphate
-
 Staphylococcus aureus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.6 3 o-nitrophenyl-beta-D-galactopyranoside 6-phosphate 2.0 hybrid with protein A Staphylococcus aureus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
lactose-6-phosphate + H2O Staphylococcus aureus
-
 D-glucose + beta-D-galactose-6-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
hybrid with protein A Staphylococcus aureus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
100
-
 maximal activity of hybrid with protein A Staphylococcus aureus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
lactose-6-phosphate + H2O
-
 Staphylococcus aureus D-glucose + beta-D-galactose-6-phosphate
-
 ?
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
-
 Staphylococcus aureus o-nitrophenol + beta-D-galactose-6-phosphate
-
 ?