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Literature summary for 3.2.1.85 extracted from

  • Witt, E.; Frank, R.; Hengstenberg, W.
    6-Phospho-beta-galactosidases of gram-positive and 6-phospho-beta-glucosidase B of gram-negative bacteria: comparison of structure and function by kinetic and immunological methods and mutagenesis of the lacG gene of Staphylococcus aureus (1993), Protein Eng., 6, 913-920.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Staphylococcus aureus
expressed in Escherichia coli Lactococcus lactis
expressed in Escherichia coli Lacticaseibacillus casei

Protein Variants

Protein Variants Comment Organism
E160Q 1000fold reduction in activity compared to wild type Staphylococcus aureus
E375Q complete loss of activity Staphylococcus aureus
G332R complete loss of catalytic activity, but same behavior during purification and in affinity to antisera as wild type Staphylococcus aureus

Inhibitors

Inhibitors Comment Organism Structure
D-galactose-6-phosphate competitive inhibition; weak inhibition Staphylococcus aureus
p-nitrophenyl-beta-thiogalactoside 6-phosphate competitive inhibition Staphylococcus aureus
p-nitrophenyl-beta-thioglucoside 6-phosphate competitive inhibition Staphylococcus aureus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
o-nitrophenyl-beta-D-glucopyranoside 6-phosphate
-
Lactococcus lactis
0.09
-
p-nitrophenyl-beta-D-galactopyranoside 6-phosphate
-
Lactococcus lactis
0.1
-
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate
-
Lactococcus lactis
0.15
-
o-nitrophenyl-beta-D-glucopyranoside 6-phosphate
-
Lacticaseibacillus casei
0.16
-
o-nitrophenyl-beta-D-glucopyranoside 6-phosphate
-
Staphylococcus aureus
0.2
-
p-nitrophenyl-beta-D-galactopyranoside 6-phosphate
-
Lacticaseibacillus casei
0.3
-
p-nitrophenyl-beta-D-galactopyranoside 6-phosphate
-
Staphylococcus aureus
0.4
-
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate
-
Lactococcus lactis
0.4
-
p-nitrophenyl-beta-D-glucopyranoside 6 phosphate
-
Staphylococcus aureus
0.6
-
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate
-
Lacticaseibacillus casei
0.6 3 o-nitrophenyl-beta-D-galactopyranoside 6-phosphate
-
Staphylococcus aureus
1.3 1.59 o-nitrophenyl-beta-D-galactopyranoside 6-phosphate
-
Lacticaseibacillus casei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
lactose-6-phosphate + H2O Staphylococcus aureus
-
D-glucose + beta-D-galactose-6-phosphate
-
?
lactose-6-phosphate + H2O Lactococcus lactis
-
D-glucose + beta-D-galactose-6-phosphate
-
?
lactose-6-phosphate + H2O Lacticaseibacillus casei
-
D-glucose + beta-D-galactose-6-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Lacticaseibacillus casei
-
-
-
Lactococcus lactis
-
-
-
Staphylococcus aureus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Staphylococcus aureus
-
Lactococcus lactis
-
Lacticaseibacillus casei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
lactose-6-phosphate + H2O
-
Staphylococcus aureus D-glucose + beta-D-galactose-6-phosphate
-
?
lactose-6-phosphate + H2O
-
Lactococcus lactis D-glucose + beta-D-galactose-6-phosphate
-
?
lactose-6-phosphate + H2O
-
Lacticaseibacillus casei D-glucose + beta-D-galactose-6-phosphate
-
?
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
-
Staphylococcus aureus o-nitrophenol + beta-D-galactose-6-phosphate
-
?
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
-
Lactococcus lactis o-nitrophenol + beta-D-galactose-6-phosphate
-
?
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
-
Lacticaseibacillus casei o-nitrophenol + beta-D-galactose-6-phosphate
-
?
o-nitrophenyl-beta-D-glucopyranoside 6-phosphate only little activity Staphylococcus aureus o-nitrophenol + beta-D-glucose-6-phosphate
-
?
o-nitrophenyl-beta-D-glucopyranoside 6-phosphate only little activity Lactococcus lactis o-nitrophenol + beta-D-glucose-6-phosphate
-
?
o-nitrophenyl-beta-D-glucopyranoside 6-phosphate only little activity Lacticaseibacillus casei o-nitrophenol + beta-D-glucose-6-phosphate
-
?
p-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
-
Staphylococcus aureus p-nitrophenol + beta-D-galactose-6-phosphate
-
?
p-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
-
Lactococcus lactis p-nitrophenol + beta-D-galactose-6-phosphate
-
?
p-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O
-
Lacticaseibacillus casei p-nitrophenol + beta-D-galactose-6-phosphate
-
?
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate only little activity Staphylococcus aureus p-nitrophenol + beta-D-glucose-6-phosphate
-
?
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate only little activity Lactococcus lactis p-nitrophenol + beta-D-glucose-6-phosphate
-
?
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate only little activity Lacticaseibacillus casei p-nitrophenol + beta-D-glucose-6-phosphate
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2
-
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate
-
Lacticaseibacillus casei
4
-
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate
-
Staphylococcus aureus
4
-
o-nitrophenyl-beta-D-glucopyranoside 6-phosphate
-
Lacticaseibacillus casei
5
-
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate
-
Lactococcus lactis
7
-
o-nitrophenyl-beta-D-glucopyranoside 6-phosphate
-
Lactococcus lactis
7
-
p-nitrophenyl-beta-D-galactopyranoside 6-phosphate
-
Lacticaseibacillus casei
9
-
o-nitrophenyl-beta-D-glucopyranoside 6-phosphate
-
Staphylococcus aureus
18
-
p-nitrophenyl-beta-D-galactopyranoside 6-phosphate
-
Staphylococcus aureus
22
-
p-nitrophenyl-beta-D-galactopyranoside 6-phosphate
-
Lactococcus lactis
57
-
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate
-
Lacticaseibacillus casei
63
-
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate
-
Staphylococcus aureus
83
-
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate
-
Lactococcus lactis