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Literature summary for 3.2.1.85 extracted from
- Purification and characterization of 6-phospho-beta-galactosidase from Lactobacillus gasseri JCM 1031 (1996), Biosci. Biotechnol. Biochem., 60, 139-141.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | - |
Lactobacillus gasseri |  |
| dithiothreitol | - |
Lactobacillus gasseri | |
| EDTA | 30% increase of activity | Lactobacillus gasseri | |
| Fe2+ | 60% increase of activity | Lactobacillus gasseri | |
| glutathione | - |
Lactobacillus gasseri | |
| L-cysteine | - |
Lactobacillus gasseri | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| iodoacetamide | - |
Lactobacillus gasseri | |
| N-ethylmaleimide | - |
Lactobacillus gasseri | |
| p-chloromercuribenzoic acid | - |
Lactobacillus gasseri | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.76 | 0.8 | o-nitrophenyl-beta-D-galactopyranoside 6-phosphate | - |
Lactobacillus gasseri | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Hg2+ | - |
Lactobacillus gasseri | |
| Zn2+ | - |
Lactobacillus gasseri | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 58000 | - |
SDS-PAGE | Lactobacillus gasseri |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| lactose-6-phosphate + H2O | Lactobacillus gasseri | - |
D-glucose + beta-D-galactose-6-phosphate | - |
? | |
| lactose-6-phosphate + H2O | Lactobacillus gasseri JCM 1031 | - |
D-glucose + beta-D-galactose-6-phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lactobacillus gasseri | - |
JCM 1031 | - |
| Lactobacillus gasseri JCM 1031 | - |
JCM 1031 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Lactobacillus gasseri |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| lactose-6-phosphate + H2O | - |
Lactobacillus gasseri | D-glucose + beta-D-galactose-6-phosphate | - |
? | |
| lactose-6-phosphate + H2O | - |
Lactobacillus gasseri JCM 1031 | D-glucose + beta-D-galactose-6-phosphate | - |
? | |
| o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O | - |
Lactobacillus gasseri | o-nitrophenol + beta-D-galactose-6-phosphate | - |
? | |
| o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O | - |
Lactobacillus gasseri JCM 1031 | o-nitrophenol + beta-D-galactose-6-phosphate | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 5.5 | - |
Lactobacillus gasseri |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 7 | at 37°C for 15 min | Lactobacillus gasseri |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 9 | at 37°C for 15 min | Lactobacillus gasseri |
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