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Literature summary for 3.2.1.85 extracted from
- Lactose metabolism involving phospho-beta-galactosidase in Klebsiella (1979), J. Bacteriol., 138, 691-698.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| D-galactose-6-phosphate | - |
Klebsiella sp. |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3 | - |
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate | with strain CT-1 | Klebsiella sp. | |
| 7.3 | - |
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate | with strain RE1755A | Klebsiella sp. | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| lactose-6-phosphate + H2O | Klebsiella sp. | - |
D-glucose + beta-D-galactose-6-phosphate | - |
? | |
| lactose-6-phosphate + H2O | Klebsiella sp. RE 1755A | - |
D-glucose + beta-D-galactose-6-phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Klebsiella sp. | - |
Lac+Gal+ mutant CT-1 | - |
| Klebsiella sp. RE 1755A | - |
Lac+Gal+ mutant CT-1 | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.004 | - |
with citrate-grown cell-extracts of CT-1 | Klebsiella sp. |
| 0.032 | - |
with lactose-grown cell-extracts of CT-1 | Klebsiella sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| lactose-6-phosphate + H2O | - |
Klebsiella sp. | D-glucose + beta-D-galactose-6-phosphate | - |
? | |
| lactose-6-phosphate + H2O | - |
Klebsiella sp. RE 1755A | D-glucose + beta-D-galactose-6-phosphate | - |
? | |
| o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O | - |
Klebsiella sp. | o-nitrophenol + beta-D-galactose-6-phosphate | - |
? | |
| o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O | - |
Klebsiella sp. RE 1755A | o-nitrophenol + beta-D-galactose-6-phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BGase-III | - |
Klebsiella sp. |
| phospho-beta-galactosidase | - |
Klebsiella sp. |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
rapid thermal inactivation, about 50% of activity after 3 min with extracts of strain CT-1, after 5 min with RE1755A | Klebsiella sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.3 | - |
- |
Klebsiella sp. |
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