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Literature summary for 3.2.1.85 extracted from
- Purification and some properties of phospho-beta-galactosidase from the Gram-negative oral bacterium Leptotrichia buccalis ATCC 14201 (2002), FEMS Microbiol. Lett., 214, 183-188.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Leptotrichia buccalis |  |
| galactose-6-phosphate | - |
Leptotrichia buccalis | |
| phosphoenolpyruvate | weak inhibition | Leptotrichia buccalis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.54 | - |
o-nitrophenyl-beta-D-galactopyranoside 6-phosphate | pH 7.5, 37°C | Leptotrichia buccalis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | not required | Leptotrichia buccalis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 53000 | - |
SDS-PAGE | Leptotrichia buccalis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| lactose-6-phosphate + H2O | Leptotrichia buccalis | - |
D-glucose + beta-D-galactose-6-phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Leptotrichia buccalis | - |
strain ATCC 14201 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Leptotrichia buccalis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 21.6 | - |
purified enzyme with o-nitrophenyl-beta-D-galactopyranoside 6-phosphate as substrate, pH 7.5, 37°C | Leptotrichia buccalis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| lactose-6-phosphate + H2O | - |
Leptotrichia buccalis | D-glucose + beta-D-galactose-6-phosphate | - |
? | |
| o-nitrophenyl-beta-D-galactopyranoside 6-phosphate + H2O | - |
Leptotrichia buccalis | o-nitrophenol + beta-D-galactose-6-phosphate | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 7.5 | - |
Leptotrichia buccalis |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Leptotrichia buccalis | isoelectric focusing | - |
4.9 |
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