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Information on EC 3.1.1.73 - feruloyl esterase and Organism(s) Acetivibrio thermocellus and UniProt Accession P10478

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EC Tree
     3 Hydrolases
         3.1 Acting on ester bonds
             3.1.1 Carboxylic-ester hydrolases
                3.1.1.73 feruloyl esterase
IUBMB Comments
Catalyses the hydrolysis of the 4-hydroxy-3-methoxycinnamoyl (feruloyl) group from an esterified sugar, which is usually arabinose in "natural" substrates. p-Nitrophenol acetate and methyl ferulate are poorer substrates. All microbial ferulate esterases are secreted into the culture medium. They are sometimes called hemicellulase accessory enzymes, since they help xylanases and pectinases to break down plant cell wall hemicellulose.
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Acetivibrio thermocellus
UNIPROT: P10478
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The taxonomic range for the selected organisms is: Acetivibrio thermocellus
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
feruloyl esterase, ferulic acid esterase, cinii, anfaea, feruloyl esterase a, fae-iii, cinnae, type a feruloyl esterase, estf27, xylanase z, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
cellulosome multi-enzyme complex
-
-
cellulosome xylanase Z feruloyl esterase
-
-
cinnAE
-
-
-
-
cinnamoyl ester hydrolase
-
-
-
-
Cinnamoyl esterase
-
-
-
-
EstA
-
-
-
-
FAE-I
-
-
-
-
FAE-II
-
-
-
-
FAE-III
-
-
-
-
FAEA
-
-
-
-
ferulic acid esterase
-
-
-
-
feruloyl esterase
-
-
feruloyl/p-coumaroyl esterase
-
-
-
-
Feruloylesterase
-
-
-
-
hemicellulase acessory enzymes
-
-
-
-
hydroxycinnamoyl esterase
-
-
-
-
phenolic acid esterase
-
-
-
-
xylanase 10
-
ferulate esterase domains are components of the multimodular xylanases 10A and 10B
xylanase 10B
-
feruloyl esterase module of cellulosomal xylanase 10B
xylanase Z
-
a domain of the Clostridium thermocellum’s cellulosomal xylanase Z displays feruloyl esterase activity
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
carboxylic ester hydrolysis
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
4-hydroxy-3-methoxycinnamoyl-sugar hydrolase
Catalyses the hydrolysis of the 4-hydroxy-3-methoxycinnamoyl (feruloyl) group from an esterified sugar, which is usually arabinose in "natural" substrates. p-Nitrophenol acetate and methyl ferulate are poorer substrates. All microbial ferulate esterases are secreted into the culture medium. They are sometimes called hemicellulase accessory enzymes, since they help xylanases and pectinases to break down plant cell wall hemicellulose.
CAS REGISTRY NUMBER
COMMENTARY hide
134712-49-5
-
224306-54-1
-
224306-55-2
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
O-beta-D-xylopyranosyl-(1,4)-O-[5-O-(trans-feruloyl)-alpha-L-arabinofuranosyl-(1,3)]-O-beta-D-xylopyranosyl-(1,4)-D-xylopyranose + H2O
O-beta-D-xylopyranosyl-(1,4)-O-alpha-L-arabinofuranosyl-(1,3)-O-beta-D-xylopyranosyl-(1,4)-D-xylopyranose + trans-ferulic acid
show the reaction diagram
-
-
-
?
5-O-[(E)-feruloyl]-[O-beta-D-xylopyranosyl-(1-2)]-O-alpha-L-arabino-furanosyl-[1-3]-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose + H2O
ferulic acid + [O-beta-D-xylopyranosyl-(1-2)]-O-alpha-L-arabino-furanosyl-[1-3]-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose
show the reaction diagram
-
-
-
-
?
5-O-[(E)-feruloyl]-[O-beta-D-xylopyranosyl-(1-2)]-O-alpha-L-arabinofuranosyl-[1-3]-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose + H2O
ferulic acid + O-beta-D-xylopyranosyl-(1-2)-O-alpha-L-arabinofuranosyl-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose
show the reaction diagram
-
-
-
-
?
coastal bermuda grass + H2O
ferulic acid + ?
show the reaction diagram
-
-
-
-
?
ethyl ferulate + H2O
ferulate + ethanol
show the reaction diagram
-
-
-
-
?
ethyl ferulate + H2O
ferulic acid + ethanol
show the reaction diagram
-
-
-
-
?
ethyl p-coumarate + H2O
p-coumaric acid + ethanol
show the reaction diagram
-
-
-
-
?
feruloyl polysaccharide + H2O
ferulic acid + ?
show the reaction diagram
-
involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components
-
-
?
feruloyl-polysaccharide + H2O
ferulate + polysaccharide
show the reaction diagram
-
-
-
-
?
hemicellulose + H2O
ferulic acid + ?
show the reaction diagram
-
involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall competents
-
-
?
hemicellulose + H2O
ferulic acid + arabinoxylan + pectin + ?
show the reaction diagram
-
involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components
-
-
?
methyl ferulate + H2O
ferulate + methanol
show the reaction diagram
-
-
-
-
?
n-decyl ferulate + H2O
ferulate + n-decanol
show the reaction diagram
-
-
-
-
?
n-hexyl ferulate + H2O
ferulate + n-hexanol
show the reaction diagram
-
-
-
-
?
n-propyl ferulate + H2O
ferulate + n-propanol
show the reaction diagram
-
-
-
-
?
O-(5-O-[(E)-feruloyl]-alpha-L-arabinofuranosyl)-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose + H2O
ferulic acid + alpha-L-arabinofuranosyl-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose
show the reaction diagram
-
-
-
-
?
O-5-O-[(E)-feruloyl]-alpha-L-arabinofuranosyl-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose + H2O
ferulic acid + alpha-L-arabinofuranosyl-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose
show the reaction diagram
-
FAXX
-
-
?
O-beta-D-xylopyranosyl-(1,4)-O-[5-O-(trans-feruloyl)-alpha-L-arabinofuranosyl-(1,3)]-O-beta-D-xylopyranosyl-(1,4)-D-xylopyranose + H2O
O-beta-D-xylopyranosyl-(1,4)-O-alpha-L-arabinofuranosyl-(1,3)-O-beta-D-xylopyranosyl-(1,4)-D-xylopyranose + trans-ferulic acid
show the reaction diagram
-
-
-
-
?
wheat bran + H2O
ferulic acid + p-coumaric acid + ?
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
5-O-[(E)-feruloyl]-[O-beta-D-xylopyranosyl-(1-2)]-O-alpha-L-arabino-furanosyl-[1-3]-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose + H2O
ferulic acid + [O-beta-D-xylopyranosyl-(1-2)]-O-alpha-L-arabino-furanosyl-[1-3]-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose
show the reaction diagram
-
-
-
-
?
coastal bermuda grass + H2O
ferulic acid + ?
show the reaction diagram
-
-
-
-
?
feruloyl polysaccharide + H2O
ferulic acid + ?
show the reaction diagram
-
involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components
-
-
?
feruloyl-polysaccharide + H2O
ferulate + polysaccharide
show the reaction diagram
-
-
-
-
?
hemicellulose + H2O
ferulic acid + ?
show the reaction diagram
-
involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall competents
-
-
?
hemicellulose + H2O
ferulic acid + arabinoxylan + pectin + ?
show the reaction diagram
-
involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components
-
-
?
O-(5-O-[(E)-feruloyl]-alpha-L-arabinofuranosyl)-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose + H2O
ferulic acid + alpha-L-arabinofuranosyl-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose
show the reaction diagram
-
-
-
-
?
wheat bran + H2O
ferulic acid + p-coumaric acid + ?
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
hydrolyzes the ester linkage between arabinofuranoside side chains and ferulic acid
-
-
?
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.578
ethyl ferulate
-
pH 7.0, 40°C
2.472
methyl ferulate
-
pH 7.0, 40°C
0.125
n-decyl ferulate
-
pH 7.0, 40°C
0.029
n-hexyl ferulate
-
pH 7.0, 40°C
1.237
n-propyl ferulate
-
pH 7.0, 40°C
5
O-beta-D-xylopyranosyl-(1,4)-O-[5-O-(trans-feruloyl)-alpha-L-arabinofuranosyl-(1,3)]-O-beta-D-xylopyranosyl-(1,4)-D-xylopyranose
-
60°C, pH 6.0
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5.7
ethyl ferulate
-
pH 7.0, 40°C
8
methyl ferulate
-
pH 7.0, 40°C
0.4
n-decyl ferulate
-
pH 7.0, 40°C
0.2
n-hexyl ferulate
-
pH 7.0, 40°C
3.2
n-propyl ferulate
-
pH 7.0, 40°C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.2
ethyl ferulate
-
pH 7.0, 40°C
3.2
methyl ferulate
-
pH 7.0, 40°C
3.2
n-decyl ferulate
-
pH 7.0, 40°C
5.6
n-hexyl ferulate
-
pH 7.0, 40°C
2.6
n-propyl ferulate
-
pH 7.0, 40°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 7
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0 - 70
-
recombinant enzyme
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2000000
-
cellulosome enzyme-complex
45000
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
crystals belong to the orthorhombic space group
-
FAE domains, such as XynY and XynZ of the cellulosomal proteins
-
hanging drop vapor diffusion method
-
hanging drop vapor diffusion method, cocrystals of the native enzyme with the substrate O-(5-O-[(E)-feruloyl]-alpha-L-arabinofuranosyl)-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose and of the S172A mutant with 5-O-[(E)-feruloyl]-[O-beta-D-xylopyranosyl-(1-2)]-O-R-L-arabino-furanosyl-[1-3]-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose
-
native and mutant protein, hanging drop vapor diffusion method
-
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
S954A
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3 - 10
-
stable
677627
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
70
-
stable up to
70 - 90
-
stable for 6h, loses 50% activity at 80°C within 3 h, loses all activity after 1 h at 90°C
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant enzyme using His-tag
-
recombinant enzymes
-
recombinant protein
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
domains XynY and XynZ containing homolgy sequences to Orpinomyces feruloyl esterase cloned and expressed in Escherichia coli
-
expression in Emericella nidulans
-
native and mutant enzyme expressed in Escherichia coli
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
degradation
-
the endo-1,4-xylanase XynC11 from Penicillium funiculosum (CAC15487)and the feruloyl esterase CE1 from Clostridium thermocellum effectively break down hemicellulose from pretreated sugarcane bagasse (up to 65%), along with the production of xylooligosaccharides GH11 and CE1 can improve biomasssaccharification by cellulases. Treatment with these two enzymes followed by a commercial cellulase cocktail increases saccharification of pretreated lignocellulose by 24%
medicine
-
potential application of ferulic acid as antioxidant and anti-cancer agent
nutrition
-
ferulic acid is a potential precursor for the synthesis of aroma and flavoring compounds
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Faulds, C.B.; Williamson, G.
Purification and characterization of a ferulic acid esterase (FAE-III) from Aspergillus niger: specificity for the phenolic moiety and binding to microcrystalline cellulose
Microbiology
140
779-787
1994
Aspergillus niger, Acetivibrio thermocellus, Talaromyces pinophilus, Pseudomonas fluorescens, Streptomyces olivochromogenes
-
Manually annotated by BRENDA team
Blum, D.L.; Kataeva, I.A.; Li, X.L.; Ljungdahl, L.G.
Feruloyl esterase activity of the Clostridium thermocellum cellulosome can be attributed to previously unknown domains of XynY and XynZ
J. Bacteriol.
182
1346-1351
2000
Aspergillus niger, Aspergillus tubingensis, Butyrivibrio fibrisolvens, Acetivibrio thermocellus, Neocallimastix sp., no activity in Escherichia coli, Orpinomyces sp., Pseudomonas fluorescens, Ruminococcus sp., Acetivibrio thermocellus JW20
Manually annotated by BRENDA team
Blum, D.L.; Schubot, F.D.; Ljungdahl, L.G.; Rose, J.P.; Wang, B.C.
Crystallization and preliminary x-ray analysis of the Clostridium thermocellum cellulosome xylanase Z feruloyl esterase domain
Acta Crystallogr. Sect. D
56
1027-1029
2000
Aspergillus niger, Aspergillus tubingensis, Butyrivibrio fibrisolvens, Acetivibrio thermocellus, Orpinomyces sp., Piromyces sp. 'equi', Pseudomonas fluorescens
-
Manually annotated by BRENDA team
Kroon, P.A.; Williamson, G.; Fish, N.M.; Archer, D.B.; Belshaw, N.J.
A modular esterase from Penicillium funiculosum which releases ferulic acid from plant cell walls and binds crystalline cellulose contains a carbohydrate binding module
Eur. J. Biochem.
267
6740-6752
2000
Aspergillus awamori, Aspergillus niger, Aspergillus sp., Butyrivibrio fibrisolvens, Acetivibrio thermocellus, Orpinomyces sp., Talaromyces funiculosus, Piromyces sp. 'equi', Talaromyces funiculosus IMI-134756
Manually annotated by BRENDA team
Tarbouriech, N.; Prates, J.A.; Fontes, C.M.; Davies, G.J.
Molecular determinants of substrate specificity in the feruloyl esterase module of xylanase 10B from Clostridium thermocellum
Acta Crystallogr. Sect. D
61
194-197
2005
Acetivibrio thermocellus
Manually annotated by BRENDA team
Schubot, F.D.; Kataeva, I.A.; Blum, D.L.; Shah, A.K.; Ljungdahl, L.G.; Rose, J.P.; Wang, B.C.
Structural basis for the substrate specificity of the feruloyl esterase domain of the cellulosomal xylanase Z from Clostridium thermocellum
Biochemistry
40
12524-12532
2001
Acetivibrio thermocellus
Manually annotated by BRENDA team
Prates, J.A.; Tarbouriech, N.; Charnock, S.J.; Fontes, C.M.; Ferreira, L.M.; Davies, G.J.
The structure of the feruloyl esterase module of xylanase 10B from Clostridium thermocellum provides insights into substrate recognition
Structure
9
1183-1190
2001
Acetivibrio thermocellus
Manually annotated by BRENDA team
Fazary, A.E.; Ju, Y.H.
Feruloyl esterases as biotechnological tools: current and future perspectives
Acta Biochim. Biophys. Sin.
39
811-828
2007
Aspergillus nidulans, Aspergillus sp., Aureobasidium pullulans, Thermothelomyces heterothallicus, Thermoclostridium stercorarium, Acetivibrio thermocellus, Fusarium oxysporum, Penicillium expansum, Talaromyces pinophilus, Talaromyces stipitatus, Pseudomonas fluorescens, Streptomyces olivochromogenes, Fusarium proliferatum, Aspergillus niger (O42807), Talaromyces funiculosus (Q9HE18), Neurospora crassa (Q9HGR3), Aspergillus awamori (Q9P979)
Manually annotated by BRENDA team
Wong, D.W.
Feruloyl esterase: a key enzyme in biomass degradation
Appl. Biochem. Biotechnol.
133
87-112
2006
Aspergillus awamori, Aspergillus oryzae, Acetivibrio thermocellus, Fusarium oxysporum, Neocallimastix sp., Penicillium expansum, Pseudomonas fluorescens, Streptomyces olivochromogenes, Aspergillus niger (O42807), Talaromyces funiculosus (Q9HE18), Neurospora crassa (Q9HGR3), Piromyces sp. 'equi' (Q9Y871)
Manually annotated by BRENDA team
Topakas, E.; Vafiadi, C.; Christakopoulos, P.
Microbial production, characterization and applications of feruloyl esterases
Process Biochem.
42
497-509
2007
Aspergillus oryzae, Aureobasidium pullulans, Aspergillus niger (O42807), Aspergillus niger (Q8WZI8), Aspergillus tubingensis (O42815), Acetivibrio thermocellus (P10478), Butyrivibrio fibrisolvens (P70884), Butyrivibrio fibrisolvens (P94315), Aspergillus nidulans (Q5BCF8), Aspergillus awamori (Q9P979)
-
Manually annotated by BRENDA team
Koseki, T.; Fushinobu, S.; Ardiansyah, S.; Shirakawa, H.; Komai, M.
Occurrence, properties, and applications of feruloyl esterases
Appl. Microbiol. Biotechnol.
84
803-810
2009
Aspergillus luchuensis, Aspergillus niger, Aspergillus niger (O42807), Aspergillus niger (Q8WZI8), Aspergillus oryzae, Aureobasidium pullulans, Thermothelomyces heterothallicus, Acetivibrio thermocellus, Fusarium oxysporum, Neocallimastix sp., Talaromyces stipitatus, Cellvibrio japonicus, Salmonella enterica subsp. enterica serovar Typhimurium (G2QND5), Aspergillus tubingensis (O42815), Penicillium chrysogenum (Q3V6C9), Aspergillus nidulans (Q5BCF8), Piromyces sp. E2 (Q870B0), Talaromyces funiculosus (Q9HE18), Neurospora crassa (Q9HGR3), Orpinomyces sp. PC-2 (Q9P8Y0), Aspergillus awamori (Q9P979), Piromyces sp. 'equi' (Q9Y871)
Manually annotated by BRENDA team
Goldbeck, R.; Goncalves, T.; Damasio, A.; Brenelli, L.; Wolf, L.; Paixao, D.; Rocha, G.; Squina, F.
Effect of hemicellulolytic enzymes to improve sugarcane bagasse saccharification and xylooligosaccharides production
J. Mol. Catal. B
131
36-46
2016
Acetivibrio thermocellus, Acetivibrio thermocellus ATCC 27405
-
Manually annotated by BRENDA team
Schaer, A.; Sprecher, I.; Topakas, E.; Faulds, C.B.; Nystroem, L.
Hydrolysis of nonpolar n-alkyl ferulates by feruloyl esterases
J. Agric. Food Chem.
64
8549-8554
2016
Acetivibrio thermocellus, rumen bacterium, Thermothelomyces thermophilus (G2QND5), Aspergillus niger (O42807), Thermothelomyces thermophilus DSM 1799 (G2QND5)
Manually annotated by BRENDA team