Cloned (Comment) | Organism |
---|---|
native and mutant enzyme expressed in Escherichia coli | Acetivibrio thermocellus |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, cocrystals of the native enzyme with the substrate O-(5-O-[(E)-feruloyl]-alpha-L-arabinofuranosyl)-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose and of the S172A mutant with 5-O-[(E)-feruloyl]-[O-beta-D-xylopyranosyl-(1-2)]-O-R-L-arabino-furanosyl-[1-3]-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose | Acetivibrio thermocellus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | a domain of the Clostridium thermocellums cellulosomal xylanase Z displays feruloyl esterase activity | Acetivibrio thermocellus | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-O-[(E)-feruloyl]-[O-beta-D-xylopyranosyl-(1-2)]-O-alpha-L-arabino-furanosyl-[1-3]-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose + H2O | Acetivibrio thermocellus | - |
ferulic acid + [O-beta-D-xylopyranosyl-(1-2)]-O-alpha-L-arabino-furanosyl-[1-3]-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose | - |
? | |
feruloyl polysaccharide + H2O | Acetivibrio thermocellus | involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components | ferulic acid + ? | - |
? | |
hemicellulose + H2O | Acetivibrio thermocellus | involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components | ferulic acid + arabinoxylan + pectin + ? | - |
? | |
O-(5-O-[(E)-feruloyl]-alpha-L-arabinofuranosyl)-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose + H2O | Acetivibrio thermocellus | - |
ferulic acid + alpha-L-arabinofuranosyl-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acetivibrio thermocellus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzymes | Acetivibrio thermocellus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-O-[(E)-feruloyl]-[O-beta-D-xylopyranosyl-(1-2)]-O-alpha-L-arabino-furanosyl-[1-3]-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose + H2O | - |
Acetivibrio thermocellus | ferulic acid + [O-beta-D-xylopyranosyl-(1-2)]-O-alpha-L-arabino-furanosyl-[1-3]-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose | - |
? | |
feruloyl polysaccharide + H2O | involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components | Acetivibrio thermocellus | ferulic acid + ? | - |
? | |
hemicellulose + H2O | involved in the degradation of plant cell wall material, breaks ferulic acid cross-links between cell wall components | Acetivibrio thermocellus | ferulic acid + arabinoxylan + pectin + ? | - |
? | |
O-(5-O-[(E)-feruloyl]-alpha-L-arabinofuranosyl)-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose + H2O | - |
Acetivibrio thermocellus | ferulic acid + alpha-L-arabinofuranosyl-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
FAE_XynZ | - |
Acetivibrio thermocellus |
xylanase Z | a domain of the Clostridium thermocellums cellulosomal xylanase Z displays feruloyl esterase activity | Acetivibrio thermocellus |