EC Number   |
|---|
   3.1.1.73 | - |
| 3.1.1.73 | by hanging drop vapor diffusion method at 20°C, to 2.5 A resolution. Crystals of recombinant FaeA enzyme and its complex with ferulic acid belong to the triclinic space group P212121, with unit cell dimensions of a = 40.04, b = 48.98, c = 61.98 A and alpha = 98.65, beta = 93.98, gamma = 103.98, and a = 41.04, b = 49.28, c = 62.23 A and alpha = 98.01, beta = 92.12, gamma = 100.11, respectively |
| 3.1.1.73 | crystals belong to the orthorhombic space group |
| 3.1.1.73 | crystals of apo-Est1E grown in 17-22% PEG3350, 0.30.5 M NaH2PO4, to 1.6 A resolution. Crystals belong to space group P21 with unit cell dimensions a = 52.0, b = 108.8, c = 91.4 A, beta = 102.28, with four Est1E molecules in the asymmetric unit. Overall structure of apo-Est1E is a canonical alpha/beta-hydrolase fold with an insertion in the loop between the 6th and 7th strands of the central beta-sheet. Crystals of Se-Met Est1E, to 2.0 A resolution, belong to space group P21 with cell dimensions a = 41.0, b = 129.6, c = 50.6 A, beta = 106.28, with two molecules in the asymmetric unit. Est1E in complex with ferulic acid, to 2.1 A resolution, crystals belong to space group C2 and with a dimer in the asymmetric unit. Overall structure of Est1E with ferulic acid bound is unchanged from the apostructure |
| 3.1.1.73 | EstA |
| 3.1.1.73 | FAE domains, such as XynY and XynZ of the cellulosomal proteins |
| 3.1.1.73 | hanging drop vapor diffusion method |
| 3.1.1.73 | hanging drop vapor diffusion method, cocrystals of the native enzyme with the substrate O-(5-O-[(E)-feruloyl]-alpha-L-arabinofuranosyl)-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose and of the S172A mutant with 5-O-[(E)-feruloyl]-[O-beta-D-xylopyranosyl-(1-2)]-O-R-L-arabino-furanosyl-[1-3]-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose |
| 3.1.1.73 | hanging drop vapor diffusion method, FAEA produced in Aspergillus niger (AnN) and Escherichia coli (EcR) |
| 3.1.1.73 | homology modeling of structure and docking of substrates |
