EC Number |
---|
3.1.1.73 | - |
3.1.1.73 | 777-bp DNA fragment digested with EcoRI and PstI and ligated into the corresponding sites of the IPTG-inducible plasmid pKK223-3, transformed into Escherichia coli strain JM109 and expressed from pKTtFAE vector |
3.1.1.73 | a chimeric enzyme comprising FaeA from Aspergillus awamori and family 42 carbohydrate-binding module from glycoside hydrolase family 54 alpha-L-arabinofuranosidase of Aspergillus kawachii |
3.1.1.73 | chimeric enzyme associating FAEA and a dockerin from Clostridium thermocellum expressed in Aspergillus niger |
3.1.1.73 | chimeric gene of FaeA and Aspergillus kawachii family 42 carbohydrate-binding module (CBM42) cloned in pPICZalphaC vector. Native and chimeric enzyme comprising FaeA-CBM42 and the Saccharomyces cerevisiae alpha-factor secretion signal peptide expressed in Pichia pastoris strain GS115 |
3.1.1.73 | DNA and amino acid sequence determination and analysis, expression of His6-tagged enzyme in Escherichia coli strain BL21 |
3.1.1.73 | DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) |
3.1.1.73 | domains XynY and XynZ containing homolgy sequences to Orpinomyces feruloyl esterase cloned and expressed in Escherichia coli |
3.1.1.73 | expressed as His-tag fusion protein in a protease deficient Aspergillus niger strain |
3.1.1.73 | expressed in Aspergillus niger strain D15-26 (pyrg-) |