EC Number   |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
|---|
   3.1.1.73 | -999 |
- |
mutations T57I, V178A, and K37I are beneficial to the thermostability of AnFaeA with 1.5-3fold improvement in half-life |
729418 |
| 3.1.1.73 | -999 |
- |
the enzyme retained 85% of its activity at 70°C while obove that temperature the catalytic ability is lost quickly |
695792 |
| 3.1.1.73 | -999 |
- |
the unfolding of the parental enzyme is irreversible on all the tested conditions, while that of the Cys235 mutants is reversible, and their ability to refold is highly dependent on the denaturing temperature. Mutants denatured at 75°C are able to efficiently reverse the unfolding to regain native structure during the cooling process |
737333 |
| 3.1.1.73 | 0 |
- |
no loss of activity upon short term storage on ice |
667273 |
| 3.1.1.73 | 20 |
40 |
stable for 1 h |
737894 |
| 3.1.1.73 | 20 |
50 |
- |
699282 |
| 3.1.1.73 | 25 |
40 |
isozyme R43, thermolabile, 30 min, loss of 50% activity at 25°C, inactivation at 40°C |
730768 |
| 3.1.1.73 | 25 |
75 |
more than 20% of the activity is retained after heating the enzyme at 85°C |
708756 |
| 3.1.1.73 | 30 |
- |
1 h, 55% residual activity |
745896 |
| 3.1.1.73 | 30 |
- |
15 min, stable |
680323 |
