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Literature summary for 3.1.1.73 extracted from
- Expression of feruloyl esterase from Aspergillus awamori in Escherichia coli: Characterization and crystal studies of the recombinant enzyme (2010), Int. J. Biol. Macromol., 46, 440-444.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| PCR products ligated to pTZ57R/T cloning vector and expressed in competent Escherichia coli TBL21 strain | Aspergillus awamori |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| by hanging drop vapor diffusion method at 20°C, to 2.5 A resolution. Crystals of recombinant FaeA enzyme and its complex with ferulic acid belong to the triclinic space group P212121, with unit cell dimensions of a = 40.04, b = 48.98, c = 61.98 A and alpha = 98.65, beta = 93.98, gamma = 103.98, and a = 41.04, b = 49.28, c = 62.23 A and alpha = 98.01, beta = 92.12, gamma = 100.11, respectively | Aspergillus awamori |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.38 | - |
ethyl ferulate | - |
Aspergillus awamori |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
x * 35000, SDS-PAGE | Aspergillus awamori |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus awamori | Q9P979 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by Ni-NTA affinity chromatography and gel filtration, with 19.57% yield | Aspergillus awamori |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.85 | - |
purified enzyme with a yield of 19.54% | Aspergillus awamori |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C | Aspergillus awamori |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ethyl ferulate + H2O | - |
Aspergillus awamori | ferulic acid + ethanol | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 35000, SDS-PAGE | Aspergillus awamori |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| FAEA | - |
Aspergillus awamori |
| feruloyl esterase | - |
Aspergillus awamori |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Aspergillus awamori |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 75 | more than 20% of the activity is retained after heating the enzyme at 85°C | Aspergillus awamori |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Aspergillus awamori |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 9 | enzyme loses more than 50% of its activity when incubated at pH less than 3.5. About 40% of the activity remains after being treated at pH below 4.0 or over 8.0 | Aspergillus awamori |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Aspergillus awamori | - |
- |
4.2 |
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Release 2023.1 (January 2023)
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