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Literature summary for 3.1.1.73 extracted from

  • Moukouli, M.; Topakas, E.; Christakopoulos, P.
    Cloning, characterization and functional expression of an alkalitolerant type C feruloyl esterase from Fusarium oxysporum (2008), Appl. Microbiol. Biotechnol., 79, 245-254.
    View publication on PubMed

Application

Application Comment Organism
food industry ferulic acid released from plant cell wall by the action of FAEs is an effective natural antioxidant with potential applications in the pharmaceutical and food industries Fusarium oxysporum
paper production FAEs could be used in pulp and paper processing and as animal feed additives to facilitate nutrient assimilation Fusarium oxysporum

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris X-33 Fusarium oxysporum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
8.2
-
methyl sinapate
-
Fusarium oxysporum
100.5
-
n-propyl ferulate
-
Fusarium oxysporum
104.7
-
iso-butyl ferulate
-
Fusarium oxysporum
104.8
-
iso-propyl ferulate
-
Fusarium oxysporum
118.1
-
ethyl ferulate
-
Fusarium oxysporum
120.4
-
methyl ferulate
-
Fusarium oxysporum
139.8
-
2-butyl ferulate
-
Fusarium oxysporum
140.6
-
4-nitrophenyl 2-O-trans-feruloyl-alpha-L-arabinofuranoside
-
Fusarium oxysporum
140.7
-
methyl caffeate
-
Fusarium oxysporum
222.9
-
methyl p-coumarate
-
Fusarium oxysporum
629.1
-
n-butyl ferulate
-
Fusarium oxysporum
3332
-
4-nitrophenyl 5-O-trans-feruloyl-alpha-L-arabinofuranoside
-
Fusarium oxysporum

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Fusarium oxysporum
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
62000
-
SDS-PAGE Fusarium oxysporum

Organism

Organism UniProt Comment Textmining
Fusarium oxysporum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ultrafiltration, anion-exchange chromatography Fusarium oxysporum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-butyl ferulate + H2O
-
Fusarium oxysporum ferulic acid + 2-butanol
-
?
4-nitrophenyl 2-O-trans-feruloyl-alpha-L-arabinofuranoside + H2O The comparison of kcat/KM ratio reveals that the enzyme hydrolyzes C-5 position slightly faster and more efficiently than C-2 position, demonstrating the broad substrate specificity of the esterase Fusarium oxysporum ferulic acid + 4-nitrophenyl-alpha-L-arabinofuranoside
-
?
4-nitrophenyl 5-O-trans-feruloyl-alpha-L-arabinofuranoside + H2O The comparison of kcat/KM ratio reveals that the enzyme hydrolyzes C-5 position slightly faster and more efficiently than C-2 position, demonstrating the broad substrate specificity of the esterase Fusarium oxysporum ferulic acid + 4-nitrophenyl alpha-L-arabinofuranoside
-
?
ethyl ferulate + H2O
-
Fusarium oxysporum ferulic acid + ethanol
-
?
iso-butyl ferulate + H2O
-
Fusarium oxysporum ferulic acid + iso-butanol
-
?
iso-propyl ferulate + H2O
-
Fusarium oxysporum ferulic acid + iso-propanol
-
?
methyl caffeate + H2O
-
Fusarium oxysporum methanol + caffeic acid
-
?
methyl ferulate + H2O
-
Fusarium oxysporum methanol + ferulic acid
-
?
methyl p-coumarate + H2O
-
Fusarium oxysporum methanol + p-coumaric acid
-
?
methyl sinapate + H2O
-
Fusarium oxysporum methanol + sinapic acid
-
?
additional information The substrate specificity profiling reveals that the enzyme is a type C FAE showing broad hydrolytic activity against the four methyl esters of hydroxycinnamic acids and strong preference for the hydrolysis of n-propyl ferulate. Fusarium oxysporum ?
-
?
n-butyl ferulate + H2O
-
Fusarium oxysporum ferulic acid + n-butanol
-
?
n-propyl ferulate + H2O
-
Fusarium oxysporum ferulic acid + n-propanol
-
?

Synonyms

Synonyms Comment Organism
Cinnamoyl esterase
-
Fusarium oxysporum
FAE
-
Fusarium oxysporum
feruloyl esterase
-
Fusarium oxysporum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
Fusarium oxysporum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
the enzyme retained 85% of its activity at 70°C while obove that temperature the catalytic ability is lost quickly Fusarium oxysporum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.12
-
methyl ferulate
-
Fusarium oxysporum
0.16
-
methyl caffeate
-
Fusarium oxysporum
0.18
-
n-propyl ferulate
-
Fusarium oxysporum
0.21
-
methyl p-coumarate
-
Fusarium oxysporum
0.26
-
iso-propyl ferulate
-
Fusarium oxysporum
0.46
-
ethyl ferulate
-
Fusarium oxysporum
0.5
-
methyl sinapate
-
Fusarium oxysporum
0.95
-
iso-butyl ferulate
-
Fusarium oxysporum
1.24
-
2-butyl ferulate
-
Fusarium oxysporum
1.55
-
4-nitrophenyl 5-O-trans-feruloyl-alpha-L-arabinofuranoside
-
Fusarium oxysporum
1.76
-
4-nitrophenyl 2-O-trans-feruloyl-alpha-L-arabinofuranoside
-
Fusarium oxysporum
14.05
-
n-butyl ferulate
-
Fusarium oxysporum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Fusarium oxysporum

pH Range

pH Minimum pH Maximum Comment Organism
4 11
-
Fusarium oxysporum

pH Stability

pH Stability pH Stability Maximum Comment Organism
additional information
-
the esterase shows broad pH stability making it an important candidate for alkaline applications such as pulp treatment in the paper industry Fusarium oxysporum
4 11 retaining more than 80% of the initial activity after 24 h Fusarium oxysporum

pI Value

Organism Comment pI Value Maximum pI Value
Fusarium oxysporum IEF-PAGE
-
6.8