Application | Comment | Organism |
---|---|---|
food industry | ferulic acid released from plant cell wall by the action of FAEs is an effective natural antioxidant with potential applications in the pharmaceutical and food industries | Fusarium oxysporum |
paper production | FAEs could be used in pulp and paper processing and as animal feed additives to facilitate nutrient assimilation | Fusarium oxysporum |
Cloned (Comment) | Organism |
---|---|
expression in Pichia pastoris X-33 | Fusarium oxysporum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8.2 | - |
methyl sinapate | - |
Fusarium oxysporum | |
100.5 | - |
n-propyl ferulate | - |
Fusarium oxysporum | |
104.7 | - |
iso-butyl ferulate | - |
Fusarium oxysporum | |
104.8 | - |
iso-propyl ferulate | - |
Fusarium oxysporum | |
118.1 | - |
ethyl ferulate | - |
Fusarium oxysporum | |
120.4 | - |
methyl ferulate | - |
Fusarium oxysporum | |
139.8 | - |
2-butyl ferulate | - |
Fusarium oxysporum | |
140.6 | - |
4-nitrophenyl 2-O-trans-feruloyl-alpha-L-arabinofuranoside | - |
Fusarium oxysporum | |
140.7 | - |
methyl caffeate | - |
Fusarium oxysporum | |
222.9 | - |
methyl p-coumarate | - |
Fusarium oxysporum | |
629.1 | - |
n-butyl ferulate | - |
Fusarium oxysporum | |
3332 | - |
4-nitrophenyl 5-O-trans-feruloyl-alpha-L-arabinofuranoside | - |
Fusarium oxysporum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Fusarium oxysporum | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
62000 | - |
SDS-PAGE | Fusarium oxysporum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Fusarium oxysporum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ultrafiltration, anion-exchange chromatography | Fusarium oxysporum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-butyl ferulate + H2O | - |
Fusarium oxysporum | ferulic acid + 2-butanol | - |
? | |
4-nitrophenyl 2-O-trans-feruloyl-alpha-L-arabinofuranoside + H2O | The comparison of kcat/KM ratio reveals that the enzyme hydrolyzes C-5 position slightly faster and more efficiently than C-2 position, demonstrating the broad substrate specificity of the esterase | Fusarium oxysporum | ferulic acid + 4-nitrophenyl-alpha-L-arabinofuranoside | - |
? | |
4-nitrophenyl 5-O-trans-feruloyl-alpha-L-arabinofuranoside + H2O | The comparison of kcat/KM ratio reveals that the enzyme hydrolyzes C-5 position slightly faster and more efficiently than C-2 position, demonstrating the broad substrate specificity of the esterase | Fusarium oxysporum | ferulic acid + 4-nitrophenyl alpha-L-arabinofuranoside | - |
? | |
ethyl ferulate + H2O | - |
Fusarium oxysporum | ferulic acid + ethanol | - |
? | |
iso-butyl ferulate + H2O | - |
Fusarium oxysporum | ferulic acid + iso-butanol | - |
? | |
iso-propyl ferulate + H2O | - |
Fusarium oxysporum | ferulic acid + iso-propanol | - |
? | |
methyl caffeate + H2O | - |
Fusarium oxysporum | methanol + caffeic acid | - |
? | |
methyl ferulate + H2O | - |
Fusarium oxysporum | methanol + ferulic acid | - |
? | |
methyl p-coumarate + H2O | - |
Fusarium oxysporum | methanol + p-coumaric acid | - |
? | |
methyl sinapate + H2O | - |
Fusarium oxysporum | methanol + sinapic acid | - |
? | |
additional information | The substrate specificity profiling reveals that the enzyme is a type C FAE showing broad hydrolytic activity against the four methyl esters of hydroxycinnamic acids and strong preference for the hydrolysis of n-propyl ferulate. | Fusarium oxysporum | ? | - |
? | |
n-butyl ferulate + H2O | - |
Fusarium oxysporum | ferulic acid + n-butanol | - |
? | |
n-propyl ferulate + H2O | - |
Fusarium oxysporum | ferulic acid + n-propanol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Cinnamoyl esterase | - |
Fusarium oxysporum |
FAE | - |
Fusarium oxysporum |
feruloyl esterase | - |
Fusarium oxysporum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Fusarium oxysporum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
the enzyme retained 85% of its activity at 70°C while obove that temperature the catalytic ability is lost quickly | Fusarium oxysporum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
methyl ferulate | - |
Fusarium oxysporum | |
0.16 | - |
methyl caffeate | - |
Fusarium oxysporum | |
0.18 | - |
n-propyl ferulate | - |
Fusarium oxysporum | |
0.21 | - |
methyl p-coumarate | - |
Fusarium oxysporum | |
0.26 | - |
iso-propyl ferulate | - |
Fusarium oxysporum | |
0.46 | - |
ethyl ferulate | - |
Fusarium oxysporum | |
0.5 | - |
methyl sinapate | - |
Fusarium oxysporum | |
0.95 | - |
iso-butyl ferulate | - |
Fusarium oxysporum | |
1.24 | - |
2-butyl ferulate | - |
Fusarium oxysporum | |
1.55 | - |
4-nitrophenyl 5-O-trans-feruloyl-alpha-L-arabinofuranoside | - |
Fusarium oxysporum | |
1.76 | - |
4-nitrophenyl 2-O-trans-feruloyl-alpha-L-arabinofuranoside | - |
Fusarium oxysporum | |
14.05 | - |
n-butyl ferulate | - |
Fusarium oxysporum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Fusarium oxysporum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 11 | - |
Fusarium oxysporum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
the esterase shows broad pH stability making it an important candidate for alkaline applications such as pulp treatment in the paper industry | Fusarium oxysporum |
4 | 11 | retaining more than 80% of the initial activity after 24 h | Fusarium oxysporum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Fusarium oxysporum | IEF-PAGE | - |
6.8 |