Activating Compound | Comment | Organism | Structure |
---|---|---|---|
oxalic acid | 5 mM, 1.4fold activation | Eleusine coracana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Fe3+ | 5 mM, complete inactivation | Eleusine coracana | |
iodoacetamide | 50 mM, 45% inhibition | Eleusine coracana | |
p-chloromercuric benzoate | 50 mM, 67% inhibition | Eleusine coracana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000053 | - |
p-nitrophenylferulate | - |
Eleusine coracana |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 5 mM, 1.4fold activation | Eleusine coracana | |
Cu+ | 5 mM, 2fold activation | Eleusine coracana | |
Cu2+ | 5 mM, 2.17fold activation | Eleusine coracana | |
Ni2+ | 5 mM, 1.4fold activation | Eleusine coracana | |
Zn2+ | 5 mM, 1.4fold activation | Eleusine coracana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
16500 | - |
gel filtration | Eleusine coracana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Eleusine coracana | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Eleusine coracana |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
26.8 | - |
- |
Eleusine coracana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | ferulic acid is enzymatically released from water-soluble polysaccharides and synthetic substrates | Eleusine coracana | ? | - |
? | |
p-nitrophenylferulate + H2O | - |
Eleusine coracana | ferulate + p-nitrophenol | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 16500, SDS-PAGE | Eleusine coracana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Eleusine coracana |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 60 | 30°C: about 60% of maximal activity, 60°C: about 65% of maximal activity | Eleusine coracana |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
15 min, stable | Eleusine coracana |
40 | - |
15 min, 20% loss of activity | Eleusine coracana |
50 | - |
15 min, about 70% of maximal activity | Eleusine coracana |
60 | - |
15 min, 50% loss of activity | Eleusine coracana |
70 | - |
15 min, 80% loss of activity | Eleusine coracana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Eleusine coracana |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8 | pH 5.5: about 70% of maximal activity, pH 8.0: about 50% of maximal activity | Eleusine coracana |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9 | 2 h, 10-20% loss of activity | Eleusine coracana |