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Literature summary for 3.1.1.73 extracted from
- Purification and characterization of a ferulic acid esterase (FAE-III) from Aspergillus niger: specificity for the phenolic moiety and binding to microcrystalline cellulose (1994), Microbiology, 140, 779-787.
No PubMed abstract available
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.36 | - |
methyl 3,4-dimethoxycinnamate | - |
Aspergillus niger |  |
| 1.44 | - |
methyl sinapinate | - |
Aspergillus niger | |
| 2.08 | - |
methyl ferulate | - |
Aspergillus niger | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 14500 | - |
gel filtration, anomalous retardation of the purified enzyme | Aspergillus niger |
| 36000 | - |
SDS-PAGE | Aspergillus niger |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| arabinoxylans + H2O | Aspergillus niger | wheat bran, barley straw, maize and sugar-cane bagasse | ferulic acid + ? | - |
? | |
| feruloyl-polysaccharide + H2O | Aspergillus niger | - |
ferulate + polysaccharide | - |
? | |
| feruloyl-polysaccharide + H2O | Pseudomonas fluorescens | - |
ferulate + polysaccharide | - |
? | |
| feruloyl-polysaccharide + H2O | Streptomyces olivochromogenes | - |
ferulate + polysaccharide | - |
? | |
| feruloyl-polysaccharide + H2O | Acetivibrio thermocellus | - |
ferulate + polysaccharide | - |
? | |
| feruloyl-polysaccharide + H2O | Talaromyces pinophilus | - |
ferulate + polysaccharide | - |
? | |
| oat spelt xylan + H2O | Aspergillus niger | - |
ferulic acid + ? | - |
? | |
| pectins + H2O | Aspergillus niger | sugar beet and spinach | ferulic acid + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acetivibrio thermocellus | - |
- |
- |
| Aspergillus niger | - |
FAE-I, FAE-II, FAE-III, strain 120.49 | - |
| Pseudomonas fluorescens | - |
- |
- |
| Streptomyces olivochromogenes | - |
- |
- |
| Talaromyces pinophilus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Aspergillus niger |
- |
Streptomyces olivochromogenes |
- |
Talaromyces pinophilus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.07 | - |
methyl p-coumarate as substrate | Aspergillus niger |
| 3 | - |
de-starched wheat bran as substrate in absence of xylanase | Aspergillus niger |
| 31 | - |
de-starched wheat bran as substrate in presence of xylanase | Aspergillus niger |
| 38.9 | - |
methyl 3,4-dimethoxycinnamate as substrate | Aspergillus niger |
| 67.2 | - |
methyl ferulate as substrate | Aspergillus niger |
| 156 | - |
methyl sinapinate as substrate | Aspergillus niger |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, stable for at least 2 months after hydrophobic interaction chromatography | Aspergillus niger |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (methyl 5-O-trans-feruloyl-alpha-L-arabinofuranoside) + H2O | chemically synthesized substrate | Aspergillus niger | ? | - |
? | |
| arabinoxylans + H2O | wheat bran, barley straw, maize and sugar-cane bagasse | Aspergillus niger | ferulic acid + ? | - |
? | |
| de-starched wheat bran + H2O | - |
Aspergillus niger | ferulic acid + ? | - |
? | |
| de-starched wheat bran + H2O | - |
Streptomyces olivochromogenes | ferulic acid + ? | - |
? | |
| de-starched wheat bran + H2O | - |
Talaromyces pinophilus | ferulic acid + ? | - |
? | |
| ferulic acid methyl ester + H2O | - |
Aspergillus niger | ferulic acid + methanol | - |
? | |
| ferulic acid methyl ester + H2O | - |
Streptomyces olivochromogenes | ferulic acid + methanol | - |
? | |
| ferulic acid methyl ester + H2O | - |
Talaromyces pinophilus | ferulic acid + methanol | - |
? | |
| feruloyl-polysaccharide + H2O | - |
Aspergillus niger | ferulate + polysaccharide | - |
? | |
| feruloyl-polysaccharide + H2O | - |
Pseudomonas fluorescens | ferulate + polysaccharide | - |
? | |
| feruloyl-polysaccharide + H2O | - |
Streptomyces olivochromogenes | ferulate + polysaccharide | - |
? | |
| feruloyl-polysaccharide + H2O | - |
Acetivibrio thermocellus | ferulate + polysaccharide | - |
? | |
| feruloyl-polysaccharide + H2O | - |
Talaromyces pinophilus | ferulate + polysaccharide | - |
? | |
| methyl sinapinate + H2O | - |
Aspergillus niger | sinapic acid + methanol | - |
? | |
| methyl sinapinate + H2O | - |
Streptomyces olivochromogenes | sinapic acid + methanol | - |
? | |
| methyl-3,4-dimethoxy cinnamate + H2O | - |
Aspergillus niger | 3,4-dimethoxycinnamic acid + methanol | - |
? | |
| additional information | - |
Acetivibrio thermocellus | ? | - |
? | |
| additional information | - |
Talaromyces pinophilus | ? | - |
? | |
| additional information | no activity against benzoate methyl esters | Streptomyces olivochromogenes | ? | - |
? | |
| additional information | benzoic acid methyl esters and methyl caffeate are no substrates, enzyme binds to microcrystalline cellulose | Aspergillus niger | ? | - |
? | |
| additional information | enzymes do not hydrolyze methyl esters of benzoic acids, vanillate and syringate | Aspergillus niger | ? | - |
? | |
| additional information | enzymes do not hydrolyze methyl esters of benzoic acids, vanillate and syringate | Pseudomonas fluorescens | ? | - |
? | |
| oat spelt xylan + H2O | - |
Aspergillus niger | ferulic acid + ? | - |
? | |
| p-coumaric acid methyl ester + H2O | - |
Aspergillus niger | p-coumaric acid + methanol | - |
? | |
| p-coumaric acid methyl ester + H2O | - |
Streptomyces olivochromogenes | p-coumaric acid + methanol | - |
? | |
| p-coumaric acid methyl ester + H2O | - |
Talaromyces pinophilus | p-coumaric acid + methanol | - |
? | |
| pectins + H2O | sugar beet and spinach | Aspergillus niger | ferulic acid + ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| acetyl/ferulic acid esterase | - |
Pseudomonas fluorescens |
| cellulosome multi-enzyme complex | - |
Acetivibrio thermocellus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | 60 | - |
Aspergillus niger |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 70 | - |
Aspergillus niger |
| 20 | 70 | - |
Streptomyces olivochromogenes |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Aspergillus niger |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
loses activity above | Streptomyces olivochromogenes |
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