Information on EC 2.7.7.1 - nicotinamide-nucleotide adenylyltransferase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea

EC NUMBER
COMMENTARY
2.7.7.1
-
RECOMMENDED NAME
GeneOntology No.
nicotinamide-nucleotide adenylyltransferase
-
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ATP + nicotinamide ribonucleotide = diphosphate + NAD+
show the reaction diagram
mechanism
-
ATP + nicotinamide ribonucleotide = diphosphate + NAD+
show the reaction diagram
mechanism
-
ATP + nicotinamide ribonucleotide = diphosphate + NAD+
show the reaction diagram
catalytic mechanism, overview
ATP + nicotinamide ribonucleotide = diphosphate + NAD+
show the reaction diagram
catalytic mechanism, overview
ATP + nicotinamide ribonucleotide = diphosphate + NAD+
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
condensation
Methanothermobacter thermautotrophicum
-
condensation
;
nucleotidyl group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
NAD biosynthesis III
-
-
NAD salvage pathway III
-
-
NAD metabolism
-
-
Nicotinate and nicotinamide metabolism
-
-
Metabolic pathways
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP:nicotinamide-nucleotide adenylyltransferase
Nicotinate nucleotide can also act as acceptor. See also EC 2.7.7.18 nicotinate-nucleotide adenylyltransferase.
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
adenosine triphosphate-nicotinamide mononucleotide transadenylase
-
-
-
-
adenylyltransferase, nicotinamide mononucleotide
-
-
-
-
Arabidopsis thaliana nicotinate/nicotinamide mononucleotide adenyltransferase
-
ATP:NMN adenylyltransferase
-
-
-
-
diphosphopyridine nucleotide pyrophosphorylase
-
-
-
-
Drosophila NMNAT
-
-
hNMNAT
-
-
hNMNAT-1
-
-
hNMNAT-2
-
-
NAD+ pyrophosphorylase
-
-
-
-
NadM-Nudix
-
-
nicotinamide 5'-mononucleotide adenylyltransferase
-
-
nicotinamide 5'-mononucleotide adenylyltransferase-2
-
-
nicotinamide 5-mononucleotide adenylyltransferase-2
-
isoform
nicotinamide adenine dinucleotide pyrophosphorylase
-
-
-
-
nicotinamide mononucleotide adenylyl-transferase
-
nicotinamide mononucleotide adenylyltransferase
-
-
-
-
nicotinamide mononucleotide adenylyltransferase
-
-
nicotinamide mononucleotide adenylyltransferase
-
-
nicotinamide mononucleotide adenylyltransferase
-
-
nicotinamide mononucleotide adenylyltransferase
-
nicotinamide mononucleotide adenylyltransferase
Methanocaldococcus jannaschii, Methanothermobacter thermautotrophicum
-
-
nicotinamide mononucleotide adenylyltransferase
-
nicotinamide mononucleotide adenylyltransferase
nicotinate nucleotide can also act as acceptor, see also EC 2.7.7.18 nicotinate-nucleotide adenylyltransferase
nicotinamide mononucleotide adenylyltransferase
-
nicotinamide mononucleotide adenylyltransferase
nicotinate nucleotide can also act as acceptor, see also EC 2.7.7.18 nicotinate-nucleotide adenylyltransferase
nicotinamide mononucleotide adenylyltransferase
-
-
nicotinamide mononucleotide adenylyltransferase 1
-
-
nicotinamide mononucleotide adenylyltransferase 1
-
-
nicotinamide mononucleotide adenylyltransferase 1
-
nicotinamide mononucleotide adenylyltransferase 2
-
nicotinamide mononucleotide adenylyltransferase 2
-
-
nicotinamide mononucleotide adenylyltransferase1
-
-
nicotinamide/nicotinic acid mononucleotide adenylyltransferase
-
nicotinate nucleotide can also act as acceptor, see also EC 2.7.7.18 nicotinate-nucleotide adenylyltransferase
nicotinamide/nicotinic acid mononucleotide adenylyltransferase
nicotinate nucleotide can also act as acceptor, see also EC 2.7.7.18 nicotinate-nucleotide adenylyltransferase
nicotinamide/nicotinic acid mononucleotide adenylyltransferase
Methanothermobacter thermautotrophicum
nicotinate nucleotide can also act as acceptor, see also EC 2.7.7.18 nicotinate-nucleotide adenylyltransferase
nicotinamide/nicotinic acid mononucleotide adenylyltransferase
-
nicotinate nucleotide can also act as acceptor, see also EC 2.7.7.18 nicotinate-nucleotide adenylyltransferase
nicotinate/nicotinamide mononucleotide adenyltransferase
-
-
nicotinate/nicotinamide mononucleotide adenyltransferase
-
NMN adenylyl transferase 1
-
-
NMN adenylyltransferase
-
-
-
-
NMN adenylyltransferase/ADP-ribose pyrophosphatase
-
NMN adenylyltransferase/ADP-ribose pyrophosphatase
-
NMN-adenylyltransferase
-
NMN/NaMN adenylyltransferase
-
-
NMN/NaMN adenylyltransferase
gene nadD
NMN/NaMN adenylyltransferase 2
-
NMN/NaMN adenylyltransferase 3
-
NMNAT
-
-
-
-
NMNAT
-
-
NMNAT
Methanothermobacter thermautotrophicum
-
-
NMNAT
Methanothermobacter thermautotrophicum
-
NMNAT-2
-
isoform
NMNAT1
isoform
NMNAT1
-
-
NMNAT1
isoform
NMNAT1
nuclear isoform
NMNAT2
isoform
NMNAT2
-
-
NMNAT2
-
isoform
NMNAT2
isoform
NMNAT3
isoform
NMNAT3
isoform
NMNAT3
mitochondrial isoform
PNAT
-
-
-
-
pyridine nucleotide adenylyltransferase
-
-
-
-
human nicotinamide mononucleotide adenylyl-transferase
-
additional information
the enzyme belongs to the (H/T)IGH motif containing nucleotidyltransferase superfamily, NadM family, bacterial NadM-Nudix subfamily
additional information
the enzyme belongs to the (H/T)IGH motif containing nucleotidyltransferase superfamily, NadM family, bacterial NadM-Nudix subfamily
CAS REGISTRY NUMBER
COMMENTARY
9032-70-6
-
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Frog
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
; isozyme NMNAT1; isozyme NMNAT1
SwissProt
Manually annotated by BRENDA team
; isozyme NMNAT2; isozyme NMNAT2
SwissProt
Manually annotated by BRENDA team
; isozyme NMNAT3; isozyme NMNAT3
SwissProt
Manually annotated by BRENDA team
isoform NMAT1
SwissProt
Manually annotated by BRENDA team
isoform NMAT2
SwissProt
Manually annotated by BRENDA team
isoform NMAT3
SwissProt
Manually annotated by BRENDA team
isoform NMNAT1
SwissProt
Manually annotated by BRENDA team
isoform NMNAT2
SwissProt
Manually annotated by BRENDA team
isoform NMNAT3
SwissProt
Manually annotated by BRENDA team
skipjack tuna
-
-
Manually annotated by BRENDA team
Methanothermobacter thermautotrophicum
-
-
-
Manually annotated by BRENDA team
Methanothermobacter thermautotrophicum
-
UniProt
Manually annotated by BRENDA team
isoform NMNAT1
SwissProt
Manually annotated by BRENDA team
isoform NMNAT2
UniProt
Manually annotated by BRENDA team
isoform NMNAT3
SwissProt
Manually annotated by BRENDA team
L1210
-
-
Manually annotated by BRENDA team
NMNAT1
SwissProt
Manually annotated by BRENDA team
NMNAT3
SwissProt
Manually annotated by BRENDA team
Mus musculus L1210
L1210
-
-
Manually annotated by BRENDA team
pheasant
-
-
-
Manually annotated by BRENDA team
brewer's yeast
-
-
Manually annotated by BRENDA team
isoform yNMNAT-1; isoform yNMNAT-2
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
malfunction
-
enzyme loss results in ubiquitination, mislocalization and aggregation of the Bruchpilot protein, and subsequent active zone degeneration
malfunction
-
absisic acid-induced oxidative stress causes stomatal cell death in a nicotinamide mononucleotide adenyltransferase mutant. Stomata partially lose their ability to close leading to drought susceptibility and the stomata are less responsive to opening cues
malfunction
-
nicotinamide mononucleotide adenylyltransferase 2 (Nmat2) homozygous deletion mutants show an approximate 60% reduction of spinal motoneurons in the lumbar region and a more than 80% reduction in the sensory neurons of the dorsal root ganglion. In the absence of Nmnat2, major target organs and tissues (e.g., muscle) are not functionally innervated resulting in perinatal lethality
physiological function
-
NMNAT appears to be a multifunctional protein that sits both at the core of central metabolism and at the crossroads of multiple cellular processes
physiological function
Methanothermobacter thermautotrophicum
NMNAT appears to be a multifunctional protein that sits both at the core of central metabolism and at the crossroads of multiple cellular processes
physiological function
-
NMNAT appears to be a multifunctional protein that sits both at the core of central metabolism and at the crossroads of multiple cellular processes
physiological function
NMNAT1 is an enzyme in the NAD biosynthetic pathway that generates NAD in the nucleus
physiological function
-
the enzyme is required for maintaining active zone structural integrity in Drosophila by interacting with the active zone protein Bruchpilot and shielding it from activity-induced ubiquitin-proteasome-mediated degradation. The enzyme specifically maintains active zone structure by direct protein-protein interaction
physiological function
-
the enzyme has both NAD synthase and chaperone function. The enzyme is essential for maintaining neuronal integrity under normal conditions and protects against several neurodegenerative conditions. Enzyme overexpression significantly suppresses both behavioral and morphological deficits associated with tauopathy by means of reducing the levels of hyperphosphorylated tau oligomers and rescueing age-dependent vacuolization induced by tau expression. The enzyme interacts with phosphorylated tau in vivo and promotes the ubiquitination and clearance of toxic tau species. Apoptosis activation is significantly reduced in brains overexpressing the enzyme, and neurodegeneration is suppressed
physiological function
-
overexpression of isoform Nmnat2 is toxic to primary neurons resulting in pervasive cell death
physiological function
-
nicotinamide mononucleotide adenylyltransferase (NMAT)is a stress response protein regulated by the heat shock factor/hypoxia-inducible factor 1alpha pathway. NMAT is a stress response protein required for thermotolerance and mitigation of oxidative stress-induced shortened lifespan
physiological function
-
the enzyme is essential for the maintenance of NAD homeostasis enabling sustainable stomatal movement. The enzyme is responsible for tolerance to stressful signalling in guard cells during stomatal closure
physiological function
-
nicotinamide mononucleotide adenylyltransferase 2 regulates axon integrity in the mouse embryo
physiological function
-
the enzyme plays a key role in NAD+ biosynthesis
physiological function
-
the enzyme plays a key role in NAD+ biosynthesis
-
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + 3-acetylpyridine-NAD+
?
show the reaction diagram
-
poor substrate
-
-
?
ATP + 3-acetylpyridine-NAD+
?
show the reaction diagram
-
reaction at 76% the rate of nicotinamide ribonucleotide
-
-
?
ATP + 3-pyridinealdehyde-NAD+
?
show the reaction diagram
-
poor substrate
-
-
?
ATP + 3-pyridinealdehyde-NAD+
?
show the reaction diagram
-
reaction at 28% the rate of nicotinamide ribonucleotide
-
-
?
ATP + beta-NMNH
diphosphate + ?
show the reaction diagram
-
-
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
-
?
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
-
?
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
-
?
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
-
?
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
-
?
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
-
?
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
-
-
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
-
-
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
-
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
-
-
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
-
-
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
-
-
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
-
-
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
?
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
-
?
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
-
-
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
-
-
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
-
-
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
-
?
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
Methanothermobacter thermautotrophicum
-
-
-
?
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
-
?
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
Frog
-
-
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
-
?
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
-
-
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
-
?
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
pheasant, Gadidae
-
-
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
?
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
-
?
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
?
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
?
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
Methanothermobacter thermautotrophicum
-
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
best substrate
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
best substrate
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
best substrate
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
best substrate
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
strongly stereospecific: beta-, not alpha-anomer of nicotinamide ribonucleotide
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
reverse reaction at 17% the rate of deamido-NAD+-synthesis, no substrates are deamino-NAD+, NADP+, adenosine diphosphoribose, 3-acetylpyridine-deamino-NAD+, 3-pyridinealdehyde-deamino-NAD+, alpha-isomer of NAD+, ITP, GTP, UTP, CTP, or TTP
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
no substrates are deamino-NAD+, NADP+, adenosine diphosphoribose, 3-acetylpyridine-deamino-NAD+, 3-pyridinealdehyde-deamino-NAD+, alpha-isomer of NAD+, ITP, GTP, UTP, CTP, or TTP
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
i.e. NMN or nicotinamide mononucleotide
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
i.e. NMN or nicotinamide mononucleotide
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
i.e. NMN or nicotinamide mononucleotide
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
i.e. NMN or nicotinamide mononucleotide
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
i.e. NMN or nicotinamide mononucleotide
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
i.e. NMN or nicotinamide mononucleotide
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
i.e. NMN or nicotinamide mononucleotide
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
i.e. NMN or nicotinamide mononucleotide
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
i.e. NMN or nicotinamide mononucleotide
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
i.e. NMN or nicotinamide mononucleotide
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
i.e. NMN or nicotinamide mononucleotide
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
i.e. NMN or nicotinamide mononucleotide
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
i.e. NMN or nicotinamide mononucleotide
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
i.e. NMN or nicotinamide mononucleotide
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
i.e. NMN or nicotinamide mononucleotide
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
i.e. NMN or nicotinamide mononucleotide
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
i.e. NMN or nicotinamide mononucleotide
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
i.e. NMN or nicotinamide mononucleotide
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
i.e. NMN or nicotinamide mononucleotide
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
i.e. NMN or nicotinamide mononucleotide
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
i.e. NMN or nicotinamide mononucleotide
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
i.e. NMN or nicotinamide mononucleotide
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
i.e. NMN or nicotinamide mononucleotide
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
i.e. NMN or nicotinamide mononucleotide
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
i.e. NMN or nicotinamide mononucleotide
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
i.e. NMN or nicotinamide mononucleotide
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
i.e. NMN or nicotinamide mononucleotide
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
i.e. NMN or nicotinamide mononucleotide
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
i.e. NMN or nicotinamide mononucleotide
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
i.e. NMN or nicotinamide mononucleotide
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
i.e. NMN or nicotinamide mononucleotide
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
Methanothermobacter thermautotrophicum
-
i.e. NMN or nicotinamide mononucleotide
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
Methanothermobacter thermautotrophicum
-
i.e. NMN or nicotinamide mononucleotide
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
Frog
-
i.e. NMN or nicotinamide mononucleotide
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
i.e. NMN or nicotinamide mononucleotide
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
i.e. NMN or nicotinamide mononucleotide
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
i.e. NMN or nicotinamide mononucleotide
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
i.e. NMN or nicotinamide mononucleotide
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
i.e. NMN or nicotinamide mononucleotide
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
i.e. NMN or nicotinamide mononucleotide
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
i.e. NMN or nicotinamide mononucleotide
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
i.e. NMN or nicotinamide mononucleotide
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
i.e. NMN or nicotinamide mononucleotide
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
pheasant, Gadidae
-
i.e. NMN or nicotinamide mononucleotide
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
i.e. NMN or nicotinamide mononucleotide
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
stereochemistry: catalyses nucleotidyl transfer from adenosine (R)-5'-triphosphate to nicotinamide mononucleotide with inversion of configuration at the alpha-phosphate giving (S)-NAD+
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
key reaction of pyridine nucleotide metabolism
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
central enzyme of NAD biosynthetic pathway
-
?
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
final step in biosynthesis of NAD+
-
?
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
isoform yNMNAT-1, isoform yNMNAT-2
-
?
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
NMNAT1
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
NMNAT2
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
NMNAT3
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
PreissHandler-independent pathway
-
?
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
the enzyme plays a key role in NAD+ biosynthesis
-
?
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
Mus musculus L1210
-
i.e. NMN or nicotinamide mononucleotide
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
the enzyme plays a key role in NAD+ biosynthesis
-
?
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
i.e. NMN or nicotinamide mononucleotide
-
r
ATP + nicotinate D-ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
i.e. nicotinic acid adenine dinucleotide
?
ATP + nicotinate mononucleotide
diphosphate + nicotinate adenine dinucleotide
show the reaction diagram
-
-
-
?
ATP + nicotinate ribonucleotide
diphosphate + nicotinic acid-adenine dinucleotide
show the reaction diagram
-
best substrate
-
r
ATP + nicotinate ribonucleotide
diphosphate + nicotinic acid-adenine dinucleotide
show the reaction diagram
-
i.e. nicotinate mononucleotide
-
r
ATP + nicotinate ribonucleotide
diphosphate + nicotinic acid-adenine dinucleotide
show the reaction diagram
-
i.e. nicotinate mononucleotide
-
r
ATP + nicotinate ribonucleotide
diphosphate + nicotinic acid-adenine dinucleotide
show the reaction diagram
-
i.e. nicotinate mononucleotide
-
r
ATP + nicotinate ribonucleotide
diphosphate + nicotinic acid-adenine dinucleotide
show the reaction diagram
-
i.e. nicotinate mononucleotide
-
r
ATP + nicotinate ribonucleotide
diphosphate + nicotinic acid-adenine dinucleotide
show the reaction diagram
-
i.e. nicotinate mononucleotide
-
r
ATP + nicotinate ribonucleotide
diphosphate + nicotinic acid-adenine dinucleotide
show the reaction diagram
-
i.e. nicotinate mononucleotide
-
r
ATP + nicotinate ribonucleotide
diphosphate + nicotinic acid-adenine dinucleotide
show the reaction diagram
i.e. nicotinate mononucleotide
-
r
ATP + nicotinate ribonucleotide
diphosphate + nicotinic acid-adenine dinucleotide
show the reaction diagram
-
i.e. nicotinate mononucleotide
-
r
ATP + nicotinate ribonucleotide
diphosphate + nicotinic acid-adenine dinucleotide
show the reaction diagram
-
i.e. nicotinate mononucleotide
-
r
ATP + nicotinate ribonucleotide
diphosphate + nicotinic acid-adenine dinucleotide
show the reaction diagram
-
i.e. nicotinate mononucleotide
-
r
ATP + nicotinate ribonucleotide
diphosphate + nicotinic acid-adenine dinucleotide
show the reaction diagram
-
i.e. nicotinate mononucleotide
-
r
ATP + nicotinate ribonucleotide
diphosphate + nicotinic acid-adenine dinucleotide
show the reaction diagram
-
i.e. nicotinate mononucleotide
-
r
ATP + nicotinate ribonucleotide
diphosphate + nicotinic acid-adenine dinucleotide
show the reaction diagram
-
i.e. nicotinate mononucleotide
-
r
ATP + nicotinate ribonucleotide
diphosphate + nicotinic acid-adenine dinucleotide
show the reaction diagram
-
i.e. nicotinate mononucleotide
-
r
ATP + nicotinate ribonucleotide
diphosphate + nicotinic acid-adenine dinucleotide
show the reaction diagram
Methanothermobacter thermautotrophicum
-
i.e. nicotinate mononucleotide
-
r
ATP + nicotinate ribonucleotide
diphosphate + nicotinic acid-adenine dinucleotide
show the reaction diagram
Frog
-
i.e. nicotinate mononucleotide
-
r
ATP + nicotinate ribonucleotide
diphosphate + nicotinic acid-adenine dinucleotide
show the reaction diagram
-
i.e. nicotinate mononucleotide
-
r
ATP + nicotinate ribonucleotide
diphosphate + nicotinic acid-adenine dinucleotide
show the reaction diagram
-
i.e. nicotinate mononucleotide
-
r
ATP + nicotinate ribonucleotide
diphosphate + nicotinic acid-adenine dinucleotide
show the reaction diagram
Anser sp., pheasant, Gadidae
-
i.e. nicotinate mononucleotide
-
r
ATP + nicotinate ribonucleotide
diphosphate + nicotinic acid-adenine dinucleotide
show the reaction diagram
i.e. nicotinate mononucleotide
-
r
ATP + nicotinate ribonucleotide
diphosphate + nicotinic acid-adenine dinucleotide
show the reaction diagram
-
less efficient
-
r
ATP + nicotinate ribonucleotide
diphosphate + nicotinic acid-adenine dinucleotide
show the reaction diagram
-
less efficient
-
r
ATP + nicotinate ribonucleotide
diphosphate + nicotinic acid-adenine dinucleotide
show the reaction diagram
-
reaction at 77% the rate of nicotinamide ribonucleotide
-
r
ATP + nicotinate ribonucleotide
diphosphate + nicotinic acid-adenine dinucleotide
show the reaction diagram
i.e. nicotinate mononucleotide
-
r
ATP + nicotinic acid mononucleotide
diphosphate + nicotinic acid adenine dinucleotide
show the reaction diagram
-
-
-
r
ATP + nicotinic acid mononucleotide
diphosphate + nicotinic acid adenine dinucleotide
show the reaction diagram
-
-
r
ATP + nicotinic acid mononucleotide
diphosphate + nicotinic acid adenine dinucleotide
show the reaction diagram
Methanothermobacter thermautotrophicum
-
-
r
ATP + nicotinic acid mononucleotide
diphosphate + deamido-NAD+
show the reaction diagram
-
-
r
ATP + NMN
diphosphate + NAD+
show the reaction diagram
-
-
?
ATP + NMN
diphosphate + NAD+
show the reaction diagram
the enzyme from Synechocystis sp. is primarily involved in NAD savage/recycling pathways
-
?
ATP + NMN
diphosphate + NAD+
show the reaction diagram
the enzyme likely plays a central role in the pathway of NAD de novo synthesis in Francisella tularensis
-
?
ATP + NMN
diphosphate + NAD+
show the reaction diagram
ATP binds before NMN with nuclear isozyme NMNAT1 and Golgi apparatus NMNAT2, but the opposite order is observed with the mitochondrial isozyme NMNAT3
-
?
ATP + NMN
diphosphate + NAD+
show the reaction diagram
the enzyme is a bifunctional NMN adenylyltransferase/ADP-ribose pyrophosphatase, structural aspects of the catalytic mechanism, overview
-
?
ATP + NMN
diphosphate + NAD+
show the reaction diagram
the enzyme is a bifunctional NMN adenylyltransferase/ADP-ribose pyrophosphatase, structural aspects of the catalytic mechanism, overview
-
?
ATP + NMNH
diphosphate + NADH
show the reaction diagram
-
-
?
ATP + tiazofurin 5'-monophosphate
?
show the reaction diagram
i.e. TrMP, substrate od isozymes NMNAT1 and NMNAT3, but not of isozyme NMNAT2
-
-
?
ATP + tiazofurin monophosphate
?
show the reaction diagram
i.e. TrMP, substrate od isozymes NMNAT1 and NMNAT3, but not of isozyme NMNAT2
-
-
?
CTP + nicotinamide ribonucleotide
diphosphate + nicotinamide cytosine dinucleotide
show the reaction diagram
-
isoform yNMNAT-2
-
?
dATP + nicotinamide ribonucleotide
diphosphate + ?
show the reaction diagram
-
isoform yNMNAT-1
-
?
dATP + nicotinamide ribonucleotide
diphosphate + nicotinamide deoxyadenosine dinucleotide
show the reaction diagram
-
isoform yNMNAT-2
-
?
deoxy-ATP + nicotinamide ribonucleotide
?
show the reaction diagram
-
-
-
-
?
deoxy-ATP + nicotinamide ribonucleotide
?
show the reaction diagram
-
-
-
-
?
deoxy-ATP + nicotinamide ribonucleotide
?
show the reaction diagram
-
-
-
-
?
deoxy-ATP + nicotinate ribonucleotide
?
show the reaction diagram
-
reaction at 18% the rate of ATP
-
-
?
deoxy-ATP + nicotinate ribonucleotide
?
show the reaction diagram
-
reaction at 30% the rate of ATP
-
-
?
GTP + nicotinamide ribonucleotide
diphosphate + NGD+
show the reaction diagram
19% of the activity with GTP, reverse reaction with 57% of the activity with NAD+, NMNA3
-
r
GTP + nicotinamide ribonucleotide
diphosphate + NGD+
show the reaction diagram
3% of the activity with GTP, reverse reaction with 5% of the activity with NAD+, NMNAT2
-
r
GTP + nicotinamide ribonucleotide
diphosphate + NGD+
show the reaction diagram
7% of the activity with GTP, reverse reaction with 26% of the activity with NAD+, NMNAT1
-
r
GTP + nicotinamide ribonucleotide
diphosphate + nicotinamide guanine dinucleotide
show the reaction diagram
-
isoform yNMNAT-2
-
?
GTP + nicotinamide ribonucleotide
nicotineamide guanine dinucleotide + diphosphate
show the reaction diagram
only isoenzyme NMNAT3
-
?
GTP + nicotinamide ribonucleotide
?
show the reaction diagram
-
-
-
?
GTP + NMN
diphosphate + NGD+
show the reaction diagram
substrates of isozyme NMNAT3
i.e. nicotinamide guanosine dinucleotide
?
ITP + nicotinamide ribonucleotide
diphosphate + nicotinamide hypoxanthine dinucleotide
show the reaction diagram
27% of the activity with ATP, NMNAT3
-
?
ITP + nicotinamide ribonucleotide
diphosphate + nicotinamide hypoxanthine dinucleotide
show the reaction diagram
5% of the activity with ATP, NMNAT2
-
?
ITP + nicotinamide ribonucleotide
diphosphate + nicotinamide hypoxanthine dinucleotide
show the reaction diagram
6% of the activity with ATP, NMNAT1
-
?
ITP + nicotinamide ribonucleotide
diphosphate + nicotinamide hypoxanthine dinucleotide
show the reaction diagram
-
isoform yNMNAT-2
-
?
ITP + nicotinamide ribonucleotide
nicotinamide hypoxanthine dinucleotide + diphosphate
show the reaction diagram
only isoenzyme NMNAT3
-
?
ITP + nicotinamide ribonucleotide
?
show the reaction diagram
-
-
-
?
NADH + diphosphate
?
show the reaction diagram
53% of the activity with NAD+, NMNAT1
-
-
?
NADH + diphosphate
?
show the reaction diagram
98% of the activity with NAD+, NMNAT2
-
-
?
NADH + diphosphate
?
show the reaction diagram
99% of the activity with NAD+, NMNAT3
-
-
?
nicotinamide hypoxanthine dinucleotide + diphosphate
?
show the reaction diagram
98% of the activity with NAD+, NMNAT3
-
-
?
nicotinamide hypoxanthine dinucleotide + diphosphate
ITP + nicotinamide ribonucleotide
show the reaction diagram
37% of the activity with NAD+, NMNAT2
-
?
nicotinamide hypoxanthine dinucleotide + diphosphate
ITP + nicotinamide ribonucleotide
show the reaction diagram
55% of the activity with NAD+, NMNAT1
-
?
nicotinate mononucleotide + ATP
nicotinate adenine dinucleotide + diphosphate
show the reaction diagram
-
-
-
-
nicotinate mononucleotide + ATP
nicotinate adenine dinucleotide + diphosphate
show the reaction diagram
PreissHandler-dependent pathway
-
?
nicotinic acid adenine dinucleotide phosphate + diphosphate
?
show the reaction diagram
93% of the activity with NAD+, NMNAT1
-
-
?
nicotinic acid adenine dinucleotide phosphate + diphosphate
?
show the reaction diagram
98% of the activity with NAD+, NMNAT2
-
-
?
nicotinic acid adenine dinucleotide phosphate + diphosphate
?
show the reaction diagram
as active as with NAD+, NMNAT3
-
-
?
nicotinic acid mononucleotide + ATP
nicotinic acid adenine dinucleotide + diphosphate
show the reaction diagram
-
-
-
?
nicotinic acid mononucleotide + ATP
nicotinic acid adenine dinucleotide + diphosphate
show the reaction diagram
-
-
-
r
nicotinic acid mononucleotide + ATP
nicotinic acid adenine dinucleotide + diphosphate
show the reaction diagram
-
-
-
nicotinic acid mononucleotide + ATP
nicotinic acid adenine dinucleotide + diphosphate
show the reaction diagram
-
-
?
nicotinic acid mononucleotide + ATP
nicotinic acid adenine dinucleotide + diphosphate
show the reaction diagram
-
-
r
nicotinic acid mononucleotide + ATP
nicotinic acid adenine dinucleotide + diphosphate
show the reaction diagram
activity observed with dATP, ITP
-
r
nicotinic acid mononucleotide + ATP
nicotinic acid adenine dinucleotide + diphosphate
show the reaction diagram
activity observed with GTP, ITP
-
r
NMN + ATP
NAD+ + diphosphate
show the reaction diagram
-
-
?
tiazofurin + ATP
tiazofurin adenine dinucleotide + diphosphate
show the reaction diagram
-
-
-
?
tiazofurin + ATP
tiazofurin adenine dinucleotide + diphosphate
show the reaction diagram
-
-
-
?
tiazofurin + ATP
tiazofurin adenine dinucleotide + diphosphate
show the reaction diagram
-
YLR010W
-
?
tiazofurin monophosphate + ATP
tiazofurin adenine dinucleotide + diphosphate
show the reaction diagram
-
-
?
ITP + NMN
diphosphate + inosine nicotinamide dinucleotide
show the reaction diagram
substrates of isozyme NMNAT3
i.e. nicotinamide hypoxanthine dinucleotide
?
additional information
?
-
-
NAD synthase nicotinamide mononucleotide adenylyltransferase is a stress-response protein that acts as a chaperone for neuronal maintenance and protection, NAD synthase nicotinamide mononucleotide adenylyltransferase displays chaperone function both in biochemical assays and cultured cells. It is upregulated in the brain upon overexpression of poly-glutamine expanded protein and recruited with the chaperone Hsp70 into protein aggregates.
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
-
?
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
-
?
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
-
-
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
-
-
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
Q9HAN9
-
-
-
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
-
-
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
-
-
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
-
-
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
-
-
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
-
-
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
-
-
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
-
-
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
Frog, Anser sp.
-
-
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
-
-
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
-
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
pheasant, Gadidae
-
-
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
Q99JR6, Q9EPA7
-
-
?
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
key reaction of pyridine nucleotide metabolism
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
Q96T66, Q9BZQ4, Q9HAN9
central enzyme of NAD biosynthetic pathway
-
?
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
final step in biosynthesis of NAD+
-
?
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
F4K687
PreissHandler-independent pathway
-
?
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
the enzyme plays a key role in NAD+ biosynthesis
-
?
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
Q57961
the enzyme plays a key role in NAD+ biosynthesis
-
?
ATP + NMN
diphosphate + NAD+
show the reaction diagram
Q96T66, Q9BZQ4, Q9HAN9
-
-
?
ATP + NMN
diphosphate + NAD+
show the reaction diagram
Q55928
the enzyme from Synechocystis sp. is primarily involved in NAD savage/recycling pathways
-
?
ATP + NMN
diphosphate + NAD+
show the reaction diagram
Q5NHR1
the enzyme likely plays a central role in the pathway of NAD de novo synthesis in Francisella tularensis
-
?
GTP + nicotinamide ribonucleotide
nicotineamide guanine dinucleotide + diphosphate
show the reaction diagram
Q96T66, Q9BZQ4, Q9HAN9
only isoenzyme NMNAT3
-
?
nicotinate mononucleotide + ATP
nicotinate adenine dinucleotide + diphosphate
show the reaction diagram
F4K687
PreissHandler-dependent pathway
-
?
nicotinic acid mononucleotide + ATP
nicotinic acid adenine dinucleotide + diphosphate
show the reaction diagram
-
-
-
?
nicotinic acid mononucleotide + ATP
nicotinic acid adenine dinucleotide + diphosphate
show the reaction diagram
Q99JR6, Q9EPA7
-
-
?
NMN + ATP
NAD+ + diphosphate
show the reaction diagram
Q96T66, Q9BZQ4, Q9HAN9
-
-
?
tiazofurin monophosphate + ATP
tiazofurin adenine dinucleotide + diphosphate
show the reaction diagram
Q96T66, Q9BZQ4, Q9HAN9
-
-
?
ITP + nicotinamide ribonucleotide
nicotinamide hypoxanthine dinucleotide + diphosphate
show the reaction diagram
Q96T66, Q9BZQ4, Q9HAN9
only isoenzyme NMNAT3
-
?
additional information
?
-
-
NAD synthase nicotinamide mononucleotide adenylyltransferase is a stress-response protein that acts as a chaperone for neuronal maintenance and protection
-
-
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Co2+
-
most effective, 0.5 mM giving the same extent of activation like 10 mM Mg2+
Co2+
-
metal ion required. Order of decreasing effectiveness: Co2+, Ni2+, Mg2+, Zn2+, Mn2+, isoform yNMNAT-2; metal ion required. Order of decreasing effectiveness: Ni2+, Zn2+, Co2+, Mg2+, Mn2+, isoform yNMNAT-1
Co2+
isoform NMNAT3 is predominantly activated by Co2+ ions (5 mM)
Divalent cations
-
requirement, particularly Mg2+, Co2+ and Ni2+, strong inhibition by some metal ions
Divalent cations
-
-
Divalent cations
-
-
KCl
-
activation, 25 mM, NAD+-synthesis, not deamido-NAD+-synthesis
Mg2+
-
requirement
Mg2+
-
requirement
Mg2+
-
Km-value: 0.035 mM; requirement
Mg2+
-
requirement
Mg2+
-
MgATP2- is the reactive substrate; requirement
Mg2+
-
requirement
Mg2+
-
optimal concentration of NMNAT-2 is 0.3 mM
Mg2+
-
metal ion required. Order of decreasing effectiveness: Co2+, Ni2+, Mg2+, Zn2+, Mn2+, isoform yNMNAT-2; metal ion required. Order of decreasing effectiveness: Ni2+, Zn2+, Co2+, Mg2+, Mn2+, isoform yNMNAT-1
Mg2+
most effective ion with isozymes NMNAT2 and NMNAT3; most effective ion with isozymes NMNAT2 and NMNAT3; most effective ion with isozymes NMNAT2 and NMNAT3; most effective ion with NMNAT2 and NMNAT3; most effective ion with NMNAT2 and NMNAT3; most effective ion with NMNAT2 and NMNAT3
Mg2+
presence of a metal ion cluster in the active site of the enzyme's Nudix domain, structure analysis and determination of the metal ion positions
Mg2+
isoform NMNAT2 is predominantly activated by Mg2+ ions (0.5 mM)
Mn2+
-
-
Mn2+
-
metal ion required. Order of decreasing effectiveness: Co2+, Ni2+, Mg2+, Zn2+, Mn2+, isoform yNMNAT-2; metal ion required. Order of decreasing effectiveness: Ni2+, Zn2+, Co2+, Mg2+, Mn2+, isoform yNMNAT-1
Ni2+
-
most effective, 0.5 mM giving the same extent of activation like 10 mM Mg2+
Ni2+
-
optimal activity at 5 mM
Ni2+
-
metal ion required. Order of decreasing effectiveness: Co2+, Ni2+, Mg2+, Zn2+, Mn2+, isoform yNMNAT-2; metal ion required. Order of decreasing effectiveness: Ni2+, Zn2+, Co2+, Mg2+, Mn2+, isoform yNMNAT-1
Zn2+
-
-
Zn2+
-
metal ion required. Order of decreasing effectiveness: Co2+, Ni2+, Mg2+, Zn2+, Mn2+, isoform yNMNAT-2; metal ion required. Order of decreasing effectiveness: Ni2+, Zn2+, Co2+, Mg2+, Mn2+, isoform yNMNAT-1
Zn2+
most effective ion with isozyme NMNAT1; most effective ion with isozyme NMNAT1; most effective ion with isozyme NMNAT1; most effective ion with NMNAT1; most effective ion with NMNAT1; most effective ion with NMNAT1
Zn2+
isoform NMNAT1 is predominantly activated by Zn2+ ions (0.05 mM)
Mn2+
presence of a metal ion cluster in the active site of the enzyme's Nudix domain, structure analysis and determination of the metal ion positions
additional information
NMNAT activity requires the presence of divalent cations.; NMNAT activity requires the presence of divalent cations.; NMNAT activity requires the presence of divalent cations.
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
5,5'-dithiobis(2-nitrobenzoic acid)
-
-
ADP
NMNAT1; NMNAT2
ADP-ribose
-
-
ADPribose
-
reverse reaction
ADPribose
-
1 mM caused 40% inhibition
Ag2+
-
IC50: 0.037 mM
AMP
-
1 mM caused 100% inhibition
beta-naphthoquinone 4-sulfonate
-
strong
beta-NMNH
-
-
Cd2+
-
-
Cd2+
-
IC50: 0.2 mM
Cr3+
-
-
Cr3+
-
IC50: 0.1 mM
Cu2+
-
strong
Cu2+
-
IC50: 0.025 mM
diphosphate
-
product inhibition
diphosphate
-
1 mM caused 90% inhibition
diphosphate
product inhibition, noncompetitive for isozyme NMNAT1, and mixed-type for isozymes NMNAT2 and NMNAT3, both versus ATP and NMN; product inhibition, noncompetitive for isozyme NMNAT1, and mixed-type for isozymes NMNAT2 and NMNAT3, both versus ATP and NMN; product inhibition, noncompetitive for isozyme NMNAT1, and mixed-type for isozymes NMNAT2 and NMNAT3, both versus ATP and NMN
Fe3+
-
-
gallotannin
IC50: 0.002 mM, NMNAT3; IC50: 0.01 mM; IC50: 0.055 mM, NMNAT2
-
gallotannin
polyphenolic plant metabolite that inhibits all three human NMNATs; polyphenolic plant metabolite that inhibits all three human NMNATs; polyphenolic plant metabolite that inhibits all three human NMNATs
-
Hg2+
-
-
Hg2+
-
IC50: 0.017 mM
N-bromosuccinimide
-
strong
NaAD+
i.e. nicotinic acid adenine dinucleotide, product inhibition, competitive for isozyme NMNAT3, and mixed-type for isozymes NMNAT1 and NMNAT2 NMN, and vice versa against ATP; i.e. nicotinic acid adenine dinucleotide, product inhibition, competitive for isozyme NMNAT3, and mixed-type for isozymes NMNAT1 and NMNAT2 NMN, and vice versa against ATP; i.e. nicotinic acid adenine dinucleotide, product inhibition, competitive for isozyme NMNAT3, and mixed-type for isozymes NMNAT1 and NMNAT2 NMN, and vice versa against ATP
NaCl
-
reverse reaction
NAD+
-
deamide-NAD+ as substrate
NAD+
product inhibition, competitive for isozyme NMNAT3, and mixed-type for isozymes NMNAT1 and NMNAT2 NMN, and vice versa against ATP; product inhibition, competitive for isozyme NMNAT3, and mixed-type for isozymes NMNAT1 and NMNAT2 NMN, and vice versa against ATP; product inhibition, competitive for isozyme NMNAT3, and mixed-type for isozymes NMNAT1 and NMNAT2 NMN, and vice versa against ATP
NAD+
product inhibition
NADP+
NMNAT1; NMNAT2
nicotinamide ribonucleotide
-
strong, nicotinate ribonucleotide as substrate
nicotinate ribonucleotide
-
nicotinamide ribonucleotide as substrate
nicotinic acid adenine dinucleotide
product inhibition
p-chloromercuribenzoate
-
-
P1-(adenosine-5')-P3-(nicotinamide ribose-5')triphosphate
-
-
P1-(adenosine-5')-P3-(nicotinamide-riboside-5')-triphosphate
-
P1-(adenosine-5')-P4-(nicotinamide ribose-5')tetraphosphate
-
no effect at 0.1 mM
P1-(adenosine-5')-P4-(nicotinamide ribose-5')tetraphosphate
-
60% inhibition at 0.1 mM
P1-(adenosine-5')-P4-(nicotinamide ribose-5')tetraphosphate
-
-
P1-(adenosine-5')-P4-(nicotinamide-riboside-5')-tetraphosphate
-
P1-(adenosine-5')-P4-(nicotinic-acid-riboside-5')-tetraphosphate
-
P1-(nicotinamide-riboside-5')-P3-(adenosine-5')-triphosphate
i.e. Np3AD; i.e. Np3AD; i.e. Np3AD
P1-(nicotinamide-riboside-5')-P4-(adenosine-5')-tetraphosphate
i.e. Np4AD; i.e. Np4AD; i.e. Np4AD
P1-(nicotinate-riboside-5')-P4-(adenosine-5')-tetraphosphate
i.e. Nap4AD; i.e. Nap4AD; i.e. Nap4AD
Metal ions
-
strong, Mg2+, Ni2+ or Co2+ activates
-
additional information
-
no inhibition by pyrimidine or purine bases, oxy- and deoxynucleosides, cAMP, halogenated and methylated nucleobases, oxy- and deoxyribose, oxy- and deoxyribose 5-phosphate, D-alpha-glucose, D-alpha-glucose 1-phosphate, D-alpha-glucose 6-phosphate, D-alpha-glucose 1,6-diphosphate, D-alpha-fructose, D-alpha-fructose 1,6-diphosphate, alpha-nicotinamide ribonucleotide; product inhibition study
-
additional information
-
no inhibition by nicotinic acid and nicotinamide
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
dithiothreitol
-
stimulates the catalytic activity of NMNAT-2 up to 30%
NH4Cl
-
activation, can substitute for KCl
SO42-
-
at 0.1 M activation
SO42-
-
0.1 M, 20fold activation
epigallocatechin gallate
0.05 mM, 10% activation, NMNAT1; 0.05 mM, 1.4fold activation, NMNAT3; 0.05 mM, 2.28fold activation, NMNAT2
additional information
-
SO42- has no effect
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.27
3-acetylpyridine-NAD+
-
37C, pH 7.5
0.74
3-pyridinealdehyde-NAD+
-
37C, pH 7.5
0.041
ADP-ribose
pH 8.2, 37C
0.0008
ATP
-
70C, pH 7.4
0.023
ATP
-
37C, pH 7.6
0.023
ATP
-
37C, pH 7.4
0.023
ATP
-
-
0.029
ATP
NMNAT3
0.0335
ATP
isoform NMNAT1, in 30 mM HEPES/KOH, pH 7.5, at 37C
0.036
ATP
-
-
0.036
ATP
-
37C
0.039
ATP
isoform NMNAT3, in 30 mM HEPES/KOH, pH 7.5, at 37C
0.04
ATP
NMNAT1
0.0421
ATP
NMNAT3; pH 7.5, 37C, isozyme NMNAT3; pH 7.5, 37C, isozyme NMNAT3; pH 7.5, 37C, isozyme NMNAT3
0.0585
ATP
NMNAT1; pH 7.5, 37C, isozyme NMNAT1; pH 7.5, 37C, isozyme NMNAT1; pH 7.5, 37C, isozyme NMNAT1
0.06
ATP
-
37C, pH 7.5, + nicotinate ribonucleotide
0.08
ATP
Methanothermobacter thermautotrophicum
-
65C, pH 7.5, H19A
0.082
ATP
isoform NMNAT2, in 30 mM HEPES/KOH, pH 7.5, at 37C
0.08425
ATP
-
wild type enzyme, in 50 mM HEPES-NaOH, pH 7.5, temperature not specified in the publication
0.088
ATP
-
pH 8
0.0889
ATP
NMNAT2; pH 7.5, 37C, isozyme NMNAT2; pH 7.5, 37C, isozyme NMNAT2; pH 7.5, 37C, isozyme NMNAT2
0.107
ATP
hNMNAT -2
0.108
ATP
-
YGR010W
0.11
ATP
-
-
0.11
ATP
-
37C, pH 7.6
0.11
ATP
-
-
0.11
ATP
-
YLR328W
0.113
ATP
-
mutant enzyme C164S/C165S, in 50 mM HEPES-NaOH, pH 7.5, temperature not specified in the publication
0.16
ATP
Methanothermobacter thermautotrophicum
-
65C, pH 7.5, wild-type
0.19
ATP
-
isoform yNMNAT-1
0.204
ATP
NMNAT2
0.21
ATP
Methanothermobacter thermautotrophicum
-
65C, pH 7.5, R11K
0.23
ATP
-
+ nicotinamide ribonucleotide, immobilized enzyme
0.33
ATP
Methanothermobacter thermautotrophicum
-
65C, pH 7.5, R11A
0.46
ATP
Methanothermobacter thermautotrophicum
-
65C, pH 7.5, R136K
0.5 - 0.52
ATP
-
37C, pH 7.5
0.5 - 0.52
ATP
-
37C, pH 7.6
1.1
ATP
Methanothermobacter thermautotrophicum
-
65C, pH 7.5, H16A
1.18
ATP
-
tiazofurin as second substrate, YGR010W
1.4
ATP
Methanothermobacter thermautotrophicum
-
65C, pH 7.5, R136A
1.4
ATP
-
isoform yNMNAT-2
0.625
beta-NMNH
-
37C, pH 7.4
0.45
dATP
-
37C
0.0045
deamido-NAD+
-
37C, pH 7.5
0.029
deamido-NAD+
-
37C, pH 7.5
0.083
diphosphate
-
isoform yNMNAT-1
0.083
diphosphate
-
0.125
diphosphate
-
37C, pH 7.6
0.167
diphosphate
-
37C, pH 7.4
0.22
diphosphate
-
-
0.39
diphosphate
NMNAT3
0.65
diphosphate
-
37C, pH 7.5
0.937
diphosphate
NMNAT1
1.1
diphosphate
-
37C, pH 7.5
1.119
diphosphate
NMNAT2
1.119
diphosphate
-
5
diphosphate
-
isoform yNMNAT-2
0.276
GTP
NMNAT3
0.023
NAD+
-
isoform yNMNAT-2
0.059
NAD+
NMNAT1
0.067 - 0.069
NAD+
-
37C, pH 7.5
0.067 - 0.069
NAD+
-
37C, pH 7.6
0.07
NAD+
NMNAT2
0.07
NAD+
-
0.073
NAD+
-
isoform yNMNAT-1
0.13
NAD+
NMNAT3
0.25
NAD+
-
-
0.37
NAD+
-
37C, pH 7.5
0.37
NAD+
-
37C, pH 7.4
0.021
nicotinamide mononucleotide
range 0.021-0.032 mM
0.17
nicotinamide mononucleotide
-
0.7
nicotinamide mononucleotide
-
0.00295
nicotinamide ribonucleotide
-
mutant enzyme C164S/C165S, in 50 mM HEPES-NaOH, pH 7.5, temperature not specified in the publication
0.00691
nicotinamide ribonucleotide
-
wild type enzyme, in 50 mM HEPES-NaOH, pH 7.5, temperature not specified in the publication
0.0252
nicotinamide ribonucleotide
isoform NMNAT1, in 30 mM HEPES/KOH, pH 7.5, at 37C
0.03
nicotinamide ribonucleotide
hNMNAT-2
0.032
nicotinamide ribonucleotide
NMNAT2
0.034
nicotinamide ribonucleotide
NMNAT1
0.038
nicotinamide ribonucleotide
-
37C, pH 7.6
0.0385
nicotinamide ribonucleotide
isoform NMNAT2, in 30 mM HEPES/KOH, pH 7.5, at 37C
0.11
nicotinamide ribonucleotide
-
isoform yNMNAT-1
0.1176
nicotinamide ribonucleotide
isoform NMNAT3, in 30 mM HEPES/KOH, pH 7.5, at 37C
0.12
nicotinamide ribonucleotide
-
-
0.13
nicotinamide ribonucleotide
-
isoform yNMNAT-2
0.147 - 0.2
nicotinamide ribonucleotide
-
37C, pH 7.5
0.147 - 0.2
nicotinamide ribonucleotide
-
37C, pH 7.6
0.147 - 0.2
nicotinamide ribonucleotide
-
37C, pH 7.6
0.147 - 0.2
nicotinamide ribonucleotide
-
-
0.209
nicotinamide ribonucleotide
NMNAT3
0.28
nicotinamide ribonucleotide
-
immobilized enzyme
0.4
nicotinamide ribonucleotide
-
37C, pH 7.5
0.08
nicotinate ribonucleotide
-
37C, pH 7.5
0.08
nicotinate ribonucleotide
-
nicotinamide ribonucleotide
0.13
nicotinate ribonucleotide
-
37C, pH 7.5
5
nicotinate ribonucleotide
-
37C, pH 7.6
5
nicotinate ribonucleotide
-
-
0.0145
nicotinic acid mononucleotide
NMNAT2; pH 7.5, 37C, isozyme NMNAT2; pH 7.5, 37C, isozyme NMNAT2; pH 7.5, 37C, isozyme NMNAT2
0.015
nicotinic acid mononucleotide
-
0.017
nicotinic acid mononucleotide
-
70C, pH 7.4
0.0677
nicotinic acid mononucleotide
NMNAT1; pH 7.5, 37C, isozyme NMNAT1; pH 7.5, 37C, isozyme NMNAT1; pH 7.5, 37C, isozyme NMNAT1
0.111
nicotinic acid mononucleotide
NMNAT3; pH 7.5, 37C, isozyme NMNAT3; pH 7.5, 37C, isozyme NMNAT3; pH 7.5, 37C, isozyme NMNAT3
0.116
nicotinic acid mononucleotide
-
-
0.116
nicotinic acid mononucleotide
-
37C
0.15
nicotinic acid mononucleotide
-
37C, pH 7.4
0.91
nicotinic acid-adenine dinucleotide
-
37C, pH 7.4
0.000019
NMN
-
37C, pH 7.6
0.000019
NMN
-
-
0.0014
NMN
-
70C, pH 7.4
0.0213
NMN
NMNAT2; pH 7.5, 37C, isozyme NMNAT2; pH 7.5, 37C, isozyme NMNAT2; pH 7.5, 37C, isozyme NMNAT2
0.0223
NMN
NMNAT1; pH 7.5, 37C, isozyme NMNAT1; pH 7.5, 37C, isozyme NMNAT1; pH 7.5, 37C, isozyme NMNAT1
0.023
NMN
-
37C
0.0662
NMN
NMNAT3; pH 7.5, 37C, isozyme NMNAT3; pH 7.5, 37C, isozyme NMNAT3; pH 7.5, 37C, isozyme NMNAT3
0.0719
NMN
-
25C, pH 8.76
0.0752
NMN
-
25C, pH 7.95
0.0791
NMN
-
25C, pH 8.44
0.08
NMN
Methanothermobacter thermautotrophicum
-
65C, pH 7.5, wild-type
0.0877
NMN
-
25C, pH 7.63
0.0879
NMN
-
25C, pH 7.16
0.09
NMN
Methanothermobacter thermautotrophicum
-
65C, pH 7.5, H19A
0.105
NMN
-
25C, pH 6.21
0.106
NMN
-
25C, pH 9.55
0.108
NMN
-
25C, pH 6.66
0.11
NMN
-
37C, pH 7.4
0.11
NMN
Methanothermobacter thermautotrophicum
-
65C, pH 7.5, R136A
0.1129
NMN
-
25C, pH 9.4
0.13
NMN
Methanothermobacter thermautotrophicum
-
65C, pH 7.5, R11K
0.139
NMN
-
25C, pH 5.85
0.14
NMN
Methanothermobacter thermautotrophicum
-
65C, pH 7.5, R136K and R11A
0.153
NMN
-
YGR010W
0.18
NMN
-
25C, pH 10
0.187
NMN
-
25C, pH 5.53
0.19
NMN
-
YLR328W
0.2
NMN
Methanothermobacter thermautotrophicum
-
65C, pH 7.5, H16A
0.201
NMN
-
25C, pH 9.77
0.298
NMN
-
25C, pH 10.35
0.336
NMN
-
25C, pH 10.55
0.45
NMN
-
25C, pH 5.15
0.594
NMN
-
25C, pH 4.95
0.13
NMNH
NMNAT3; pH 7.5, 37C, isozyme NMNAT3; pH 7.5, 37C, isozyme NMNAT3; pH 7.5, 37C, isozyme NMNAT3
0.294
NMNH
NMNAT1; pH 7.5, 37C, isozyme NMNAT1; pH 7.5, 37C, isozyme NMNAT1; pH 7.5, 37C, isozyme NMNAT1
0.304
NMNH
NMNAT2; pH 7.5, 37C, isozyme NMNAT2; pH 7.5, 37C, isozyme NMNAT2; pH 7.5, 37C, isozyme NMNAT2
0.0304
reduced nicotinamide mononucleotide
-
0.055
tiazofurin
-
YGR010W
0.37
tiazofurin monophosphate
NMNAT1
2.01
tiazofurin monophosphate
NMNAT3
100
tiazofurin monophosphate
NMNAT2
0.37
tiazofurin riboside 5'-monophosphate
pH 7.5, 37C, isozyme NMNAT1; pH 7.5, 37C, isozyme NMNAT1; pH 7.5, 37C, isozyme NMNAT1
2.1
tiazofurin riboside 5'-monophosphate
pH 7.5, 37C, isozyme NMNAT3; pH 7.5, 37C, isozyme NMNAT3; pH 7.5, 37C, isozyme NMNAT3
100
tiazofurin riboside 5'-monophosphate
above, pH 7.5, 37C, isozyme NMNAT2; above, pH 7.5, 37C, isozyme NMNAT2; above, pH 7.5, 37C, isozyme NMNAT2
additional information
additional information
initial and steady-state kinetics, kinetic mechanisms of the three NMNAT isozymes, overview; initial and steady-state kinetics, kinetic mechanisms of the three NMNAT isozymes, overview; initial and steady-state kinetics, kinetic mechanisms of the three NMNAT isozymes, overview
-
additional information
additional information
steady state kinetics
-
additional information
additional information
steady state kinetics
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
3.6
ADP-ribose
Francisella tularensis
Q5NHR1
pH 8.2, 37C
0.0000333
ATP
Methanothermobacter thermautotrophicum
-
65C, pH 7.5, H19A
0.000117
ATP
Methanothermobacter thermautotrophicum
-
65C, pH 7.5, R136A
0.000133
ATP
Methanothermobacter thermautotrophicum
-
65C, pH 7.5, R11A
0.000167
ATP
Methanothermobacter thermautotrophicum
-
65C, pH 7.5, H16A
0.0008
ATP
Methanothermobacter thermautotrophicum
-
65C, pH 7.5, R11K
0.00222
ATP
Methanothermobacter thermautotrophicum
-
65C, pH 7.5, R136K
0.00278
ATP
Methanothermobacter thermautotrophicum
-
65C, pH 7.5, wild-type
0.74
ATP
Mus musculus
Q5HZI3, Q99JR6, Q9EPA7
isoform NMNAT3, in 30 mM HEPES/KOH, pH 7.5, at 37C
2.33
ATP
Mus musculus
Q5HZI3, Q99JR6, Q9EPA7
isoform NMNAT2, in 30 mM HEPES/KOH, pH 7.5, at 37C
2.5
ATP
Homo sapiens
Q96T66, Q9BZQ4, Q9HAN9
pH 7.5, 37C, isozyme NMNAT3; pH 7.5, 37C, isozyme NMNAT3; pH 7.5, 37C, isozyme NMNAT3
8.8
ATP
Homo sapiens
Q96T66, Q9BZQ4, Q9HAN9
pH 7.5, 37C, isozyme NMNAT2; pH 7.5, 37C, isozyme NMNAT2; pH 7.5, 37C, isozyme NMNAT2
15
ATP
Mus musculus
Q5HZI3, Q99JR6, Q9EPA7
isoform NMNAT1, in 30 mM HEPES/KOH, pH 7.5, at 37C
53.9
ATP
Homo sapiens
Q96T66, Q9BZQ4, Q9HAN9
pH 7.5, 37C, isozyme NMNAT1; pH 7.5, 37C, isozyme NMNAT1; pH 7.5, 37C, isozyme NMNAT1
0.654
nicotinamide ribonucleotide
Mus musculus
-
wild type enzyme, in 50 mM HEPES-NaOH, pH 7.5, temperature not specified in the publication
0.75
nicotinamide ribonucleotide
Mus musculus
Q5HZI3, Q99JR6, Q9EPA7
isoform NMNAT3, in 30 mM HEPES/KOH, pH 7.5, at 37C
2.87
nicotinamide ribonucleotide
Mus musculus
Q5HZI3, Q99JR6, Q9EPA7
isoform NMNAT2, in 30 mM HEPES/KOH, pH 7.5, at 37C
7.21
nicotinamide ribonucleotide
Mus musculus
-
mutant enzyme C164S/C165S, in 50 mM HEPES-NaOH, pH 7.5, temperature not specified in the publication
15.5
nicotinamide ribonucleotide
Mus musculus
Q5HZI3, Q99JR6, Q9EPA7
isoform NMNAT1, in 30 mM HEPES/KOH, pH 7.5, at 37C
3.8
nicotinic acid mononucleotide
Homo sapiens
Q96T66, Q9BZQ4, Q9HAN9
pH 7.5, 37C, isozyme NMNAT3; pH 7.5, 37C, isozyme NMNAT3; pH 7.5, 37C, isozyme NMNAT3
6.9
nicotinic acid mononucleotide
Homo sapiens
Q96T66, Q9BZQ4, Q9HAN9
pH 7.5, 37C, isozyme NMNAT2; pH 7.5, 37C, isozyme NMNAT2; pH 7.5, 37C, isozyme NMNAT2
42.9
nicotinic acid mononucleotide
Homo sapiens
Q96T66, Q9BZQ4, Q9HAN9
pH 7.5, 37C, isozyme NMNAT1; pH 7.5, 37C, isozyme NMNAT1; pH 7.5, 37C, isozyme NMNAT1
2.5
NMN
Homo sapiens
Q96T66, Q9BZQ4, Q9HAN9
pH 7.5, 37C, isozyme NMNAT3; pH 7.5, 37C, isozyme NMNAT3; pH 7.5, 37C, isozyme NMNAT3
8.8
NMN
Homo sapiens
Q96T66, Q9BZQ4, Q9HAN9
pH 7.5, 37C, isozyme NMNAT2; pH 7.5, 37C, isozyme NMNAT2; pH 7.5, 37C, isozyme NMNAT2
53.8
NMN
Homo sapiens
Q96T66, Q9BZQ4, Q9HAN9
pH 7.5, 37C, isozyme NMNAT1; pH 7.5, 37C, isozyme NMNAT1; pH 7.5, 37C, isozyme NMNAT1
3.3
NMNH
Homo sapiens
Q96T66, Q9BZQ4, Q9HAN9
pH 7.5, 37C, isozyme NMNAT2; pH 7.5, 37C, isozyme NMNAT2; pH 7.5, 37C, isozyme NMNAT2
7.2
NMNH
Homo sapiens
Q96T66, Q9BZQ4, Q9HAN9
pH 7.5, 37C, isozyme NMNAT3; pH 7.5, 37C, isozyme NMNAT3; pH 7.5, 37C, isozyme NMNAT3
18.6
NMNH
Homo sapiens
Q96T66, Q9BZQ4, Q9HAN9
pH 7.5, 37C, isozyme NMNAT1; pH 7.5, 37C, isozyme NMNAT1; pH 7.5, 37C, isozyme NMNAT1
0.42
tiazofurin riboside 5'-monophosphate
Homo sapiens
Q96T66, Q9BZQ4, Q9HAN9
pH 7.5, 37C, isozyme NMNAT3; pH 7.5, 37C, isozyme NMNAT3; pH 7.5, 37C, isozyme NMNAT3
0.55
tiazofurin riboside 5'-monophosphate
Homo sapiens
Q96T66, Q9BZQ4, Q9HAN9
pH 7.5, 37C, isozyme NMNAT2; pH 7.5, 37C, isozyme NMNAT2; pH 7.5, 37C, isozyme NMNAT2
14.9
tiazofurin riboside 5'-monophosphate
Homo sapiens
Q96T66, Q9BZQ4, Q9HAN9
pH 7.5, 37C, isozyme NMNAT1; pH 7.5, 37C, isozyme NMNAT1; pH 7.5, 37C, isozyme NMNAT1
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
20
ATP
Mus musculus
Q5HZI3, Q99JR6, Q9EPA7
isoform NMNAT3, in 30 mM HEPES/KOH, pH 7.5, at 37C
4
30
ATP
Mus musculus
Q5HZI3, Q99JR6, Q9EPA7
isoform NMNAT2, in 30 mM HEPES/KOH, pH 7.5, at 37C
4
450
ATP
Mus musculus
Q5HZI3, Q99JR6, Q9EPA7
isoform NMNAT1, in 30 mM HEPES/KOH, pH 7.5, at 37C
4
10
nicotinamide ribonucleotide
Mus musculus
Q5HZI3, Q99JR6, Q9EPA7
isoform NMNAT3, in 30 mM HEPES/KOH, pH 7.5, at 37C
1685
70
nicotinamide ribonucleotide
Mus musculus
Q5HZI3, Q99JR6, Q9EPA7
isoform NMNAT2, in 30 mM HEPES/KOH, pH 7.5, at 37C
1685
610
nicotinamide ribonucleotide
Mus musculus
Q5HZI3, Q99JR6, Q9EPA7
isoform NMNAT1, in 30 mM HEPES/KOH, pH 7.5, at 37C
1685
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.16
diphosphate
product inhibition of NMNAT2, substrate NMN
0.175
diphosphate
product inhibition of NMNAT1, substrate ATP
0.334
diphosphate
product inhibition of NMNAT3, substrate ATP
0.361
diphosphate
product inhibition of NMNAT2, substrate ATP
0.39
diphosphate
product inhibition of NMNAT1, substrate NMN
1
diphosphate
product inhibition of NMNAT3, substrate NMN
0.023
NaAD+
product inhibition of NMNAT2, substrate ATP
0.041
NaAD+
product inhibition of NMNAT2, substrate NMN
0.205
NaAD+
product inhibition of NMNAT3, substrate ATP
0.305
NaAD+
product inhibition of NMNAT1, substrate ATP
0.364
NaAD+
product inhibition of NMNAT3, substrate NMN
0.502
NaAD+
product inhibition of NMNAT1, substrate NMN
0.067
NAD+
product inhibition of NMNAT2, substrate NMN
0.095
NAD+
product inhibition of NMNAT2, substrate ATP
0.418
NAD+
product inhibition of NMNAT1, substrate NMN
0.749
NAD+
product inhibition of NMNAT1, substrate ATP
1.017
NAD+
product inhibition of NMNAT3, substrate NMN
1.418
NAD+
product inhibition of NMNAT3, substrate ATP
0.0315
P1-(adenosine-5')-P3-(nicotinamide-riboside-5')-triphosphate
substrate: NMN, isoenzyme: NMNAT2
0.0359
P1-(adenosine-5')-P3-(nicotinamide-riboside-5')-triphosphate
substrate: ATP, isoenzyme: NMNAT2
0.0406
P1-(adenosine-5')-P3-(nicotinamide-riboside-5')-triphosphate
substrate: ATP, isoenzyme: NMNAT3
0.0563
P1-(adenosine-5')-P3-(nicotinamide-riboside-5')-triphosphate
substrate: ATP, isoenzyme: NMNAT1
0.0668
P1-(adenosine-5')-P3-(nicotinamide-riboside-5')-triphosphate
substrate: NMN, isoenzyme: NMNAT3
0.089
P1-(adenosine-5')-P3-(nicotinamide-riboside-5')-triphosphate
substrate: NMN, isoenzyme: NMNAT1
0.0328
P1-(adenosine-5')-P3-(nicotinic-acid-riboside-5')-triphosphate
substrate: ATP, isoenzyme: NMNAT3
0.0591
P1-(adenosine-5')-P3-(nicotinic-acid-riboside-5')-triphosphate
substrate: ATP, isoenzyme: NMNAT1
0.0679
P1-(adenosine-5')-P3-(nicotinic-acid-riboside-5')-triphosphate
substrate: NMN, isoenzyme: NMNAT1
0.0883
P1-(adenosine-5')-P3-(nicotinic-acid-riboside-5')-triphosphate
substrate: NMN, isoenzyme: NMNAT3
0.1745
P1-(adenosine-5')-P3-(nicotinic-acid-riboside-5')-triphosphate
substrate: ATP, isoenzyme: NMNAT2
0.3283
P1-(adenosine-5')-P3-(nicotinic-acid-riboside-5')-triphosphate
substrate: NMN, isoenzyme: NMNAT2
0.0242
P1-(adenosine-5')-P4-(nicotinamide-riboside-5')-tetraphosphate
substrate: ATP, isoenzyme: NMNAT2
0.0258
P1-(adenosine-5')-P4-(nicotinamide-riboside-5')-tetraphosphate
substrate: NMN, isoenzyme: NMNAT2
0.0298
P1-(adenosine-5')-P4-(nicotinamide-riboside-5')-tetraphosphate
substrate: ATP, isoenzyme: NMNAT3
0.0311
P1-(adenosine-5')-P4-(nicotinamide-riboside-5')-tetraphosphate
substrate: NMN, isoenzyme: NMNAT1
0.0492
P1-(adenosine-5')-P4-(nicotinamide-riboside-5')-tetraphosphate
substrate: ATP, isoenzyme: NMNAT1
0.0736
P1-(adenosine-5')-P4-(nicotinamide-riboside-5')-tetraphosphate
substrate: NMN, isoenzyme: NMNAT3
0.0217
P1-(adenosine-5')-P4-(nicotinic-acid-riboside-5')-tetraphosphate
substrate: NMN, isoenzyme: NMNAT3
0.0369
P1-(adenosine-5')-P4-(nicotinic-acid-riboside-5')-tetraphosphate
substrate: NMN, isoenzyme: NMNAT1
0.0431
P1-(adenosine-5')-P4-(nicotinic-acid-riboside-5')-tetraphosphate
substrate: ATP, isoenzyme: NMNAT3
0.0845
P1-(adenosine-5')-P4-(nicotinic-acid-riboside-5')-tetraphosphate
substrate: ATP, isoenzyme: NMNAT1
0.1257
P1-(adenosine-5')-P4-(nicotinic-acid-riboside-5')-tetraphosphate
substrate: NMN, isoenzyme: NMNAT2
0.6576
P1-(adenosine-5')-P4-(nicotinic-acid-riboside-5')-tetraphosphate
substrate: ATP, isoenzyme: NMNAT2
0.0315
P1-(nicotinamide-riboside-5')-P3-(adenosine-5')-triphosphate
pH 7.5, 37C, isozyme NMNAT2, versus substrate NMN; pH 7.5, 37C, isozyme NMNAT2, versus substrate NMN; pH 7.5, 37C, isozyme NMNAT2, versus substrate NMN
0.0359
P1-(nicotinamide-riboside-5')-P3-(adenosine-5')-triphosphate
pH 7.5, 37C, isozyme NMNAT2, versus substrate ATP; pH 7.5, 37C, isozyme NMNAT2, versus substrate ATP; pH 7.5, 37C, isozyme NMNAT2, versus substrate ATP
0.0406
P1-(nicotinamide-riboside-5')-P3-(adenosine-5')-triphosphate
pH 7.5, 37C, isozyme NMNAT3, versus substrate ATP; pH 7.5, 37C, isozyme NMNAT3, versus substrate ATP; pH 7.5, 37C, isozyme NMNAT3, versus substrate ATP
0.0563
P1-(nicotinamide-riboside-5')-P3-(adenosine-5')-triphosphate
pH 7.5, 37C, isozyme NMNAT1, versus substrate ATP; pH 7.5, 37C, isozyme NMNAT1, versus substrate ATP; pH 7.5, 37C, isozyme NMNAT1, versus substrate ATP
0.0668
P1-(nicotinamide-riboside-5')-P3-(adenosine-5')-triphosphate
pH 7.5, 37C, isozyme NMNAT3, versus substrate NMN; pH 7.5, 37C, isozyme NMNAT3, versus substrate NMN; pH 7.5, 37C, isozyme NMNAT3, versus substrate NMN
0.089
P1-(nicotinamide-riboside-5')-P3-(adenosine-5')-triphosphate
pH 7.5, 37C, isozyme NMNAT1, versus substrate NMN; pH 7.5, 37C, isozyme NMNAT1, versus substrate NMN; pH 7.5, 37C, isozyme NMNAT1, versus substrate NMN
0.0242
P1-(nicotinamide-riboside-5')-P4-(adenosine-5')-tetraphosphate
pH 7.5, 37C, isozyme NMNAT2, versus substrate ATP; pH 7.5, 37C, isozyme NMNAT2, versus substrate ATP; pH 7.5, 37C, isozyme NMNAT2, versus substrate ATP
0.0258
P1-(nicotinamide-riboside-5')-P4-(adenosine-5')-tetraphosphate
pH 7.5, 37C, isozyme NMNAT2, versus substrate NMN; pH 7.5, 37C, isozyme NMNAT2, versus substrate NMN; pH 7.5, 37C, isozyme NMNAT2, versus substrate NMN
0.0298
P1-(nicotinamide-riboside-5')-P4-(adenosine-5')-tetraphosphate
pH 7.5, 37C, isozyme NMNAT3, versus substrate ATP; pH 7.5, 37C, isozyme NMNAT3, versus substrate ATP; pH 7.5, 37C, isozyme NMNAT3, versus substrate ATP
0.0311
P1-(nicotinamide-riboside-5')-P4-(adenosine-5')-tetraphosphate
pH 7.5, 37C, isozyme NMNAT1, versus substrate NMN; pH 7.5, 37C, isozyme NMNAT1, versus substrate NMN; pH 7.5, 37C, isozyme NMNAT1, versus substrate NMN
0.0492
P1-(nicotinamide-riboside-5')-P4-(adenosine-5')-tetraphosphate
pH 7.5, 37C, isozyme NMNAT1, versus substrate ATP; pH 7.5, 37C, isozyme NMNAT1, versus substrate ATP; pH 7.5, 37C, isozyme NMNAT1, versus substrate ATP
0.0736
P1-(nicotinamide-riboside-5')-P4-(adenosine-5')-tetraphosphate
pH 7.5, 37C, isozyme NMNAT3, versus substrate NMN; pH 7.5, 37C, isozyme NMNAT3, versus substrate NMN; pH 7.5, 37C, isozyme NMNAT3, versus substrate NMN
0.0217
P1-(nicotinate-riboside-5')-P4-(adenosine-5')-tetraphosphate
pH 7.5, 37C, isozyme NMNAT3, versus substrate NaMN; pH 7.5, 37C, isozyme NMNAT3, versus substrate NaMN; pH 7.5, 37C, isozyme NMNAT3, versus substrate NaMN
0.0328
P1-(nicotinate-riboside-5')-P4-(adenosine-5')-tetraphosphate
pH 7.5, 37C, isozyme NMNAT3, versus substrate ATP; pH 7.5, 37C, isozyme NMNAT3, versus substrate ATP; pH 7.5, 37C, isozyme NMNAT3, versus substrate ATP
0.0369
P1-(nicotinate-riboside-5')-P4-(adenosine-5')-tetraphosphate
pH 7.5, 37C, isozyme NMNAT1, versus substrate NaMN; pH 7.5, 37C, isozyme NMNAT1, versus substrate NaMN; pH 7.5, 37C, isozyme NMNAT1, versus substrate NaMN
0.0431
P1-(nicotinate-riboside-5')-P4-(adenosine-5')-tetraphosphate
pH 7.5, 37C, isozyme NMNAT3, versus substrate ATP; pH 7.5, 37C, isozyme NMNAT3, versus substrate ATP; pH 7.5, 37C, isozyme NMNAT3, versus substrate ATP
0.0591
P1-(nicotinate-riboside-5')-P4-(adenosine-5')-tetraphosphate
pH 7.5, 37C, isozyme NMNAT1, versus substrate ATP; pH 7.5, 37C, isozyme NMNAT1, versus substrate ATP; pH 7.5, 37C, isozyme NMNAT1, versus substrate ATP
0.0679
P1-(nicotinate-riboside-5')-P4-(adenosine-5')-tetraphosphate
pH 7.5, 37C, isozyme NMNAT1, versus substrate NMN; pH 7.5, 37C, isozyme NMNAT1, versus substrate NMN; pH 7.5, 37C, isozyme NMNAT1, versus substrate NMN
0.0845
P1-(nicotinate-riboside-5')-P4-(adenosine-5')-tetraphosphate
pH 7.5, 37C, isozyme NMNAT1, versus substrate ATP; pH 7.5, 37C, isozyme NMNAT1, versus substrate ATP; pH 7.5, 37C, isozyme NMNAT1, versus substrate ATP
0.0883
P1-(nicotinate-riboside-5')-P4-(adenosine-5')-tetraphosphate
pH 7.5, 37C, isozyme NMNAT3, versus substrate NMN; pH 7.5, 37C, isozyme NMNAT3, versus substrate NMN; pH 7.5, 37C, isozyme NMNAT3, versus substrate NMN
0.1257
P1-(nicotinate-riboside-5')-P4-(adenosine-5')-tetraphosphate
pH 7.5, 37C, isozyme NMNAT2, versus substrate NaMN; pH 7.5, 37C, isozyme NMNAT2, versus substrate NaMN; pH 7.5, 37C, isozyme NMNAT2, versus substrate NaMN
0.1745
P1-(nicotinate-riboside-5')-P4-(adenosine-5')-tetraphosphate
pH 7.5, 37C, isozyme NMNAT2, versus substrate ATP; pH 7.5, 37C, isozyme NMNAT2, versus substrate ATP; pH 7.5, 37C, isozyme NMNAT2, versus substrate ATP
0.3283
P1-(nicotinate-riboside-5')-P4-(adenosine-5')-tetraphosphate
pH 7.5, 37C, isozyme NMNAT2, versus substrate NMN; pH 7.5, 37C, isozyme NMNAT2, versus substrate NMN; pH 7.5, 37C, isozyme NMNAT2, versus substrate NMN
0.6576
P1-(nicotinate-riboside-5')-P4-(adenosine-5')-tetraphosphate
pH 7.5, 37C, isozyme NMNAT2, versus substrate ATP; pH 7.5, 37C, isozyme NMNAT2, versus substrate ATP; pH 7.5, 37C, isozyme NMNAT2, versus substrate ATP
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.037
Ag2+
Bos taurus
-
IC50: 0.037 mM
0.2
Cd2+
Bos taurus
-
IC50: 0.2 mM
0.1
Cr3+
Bos taurus
-
IC50: 0.1 mM
0.025
Cu2+
Bos taurus
-
IC50: 0.025 mM
0.002
gallotannin
Homo sapiens
Q96T66, Q9HAN9
IC50: 0.002 mM, NMNAT3
-
0.01
gallotannin
Homo sapiens
Q96T66, Q9HAN9
IC50: 0.01 mM
-
0.055
gallotannin
Homo sapiens
Q96T66, Q9HAN9
IC50: 0.055 mM, NMNAT2
-
0.017
Hg2+
Bos taurus
-
IC50: 0.017 mM
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.0038
-
NAD+-synthesis
0.004
-
NAD+ as substrate
0.024
-
deamido-NAD+ as substrate
0.068
-
deamido-NAD+-synthesis
0.11
-
immobilized enzyme, reverse reaction
0.112
-
tiazofurin as substrate, YGR010W
2.8
-
purification step: Matrix Gel Green A
3.3
-
NAD+-synthesis
3.85
-
deamido-NAD+ as substrate
5
-
NAD+ as substrate, yeast
7.3
-
deamido-NAD+-synthesis
8.8
-
purification step: TSK phenyl-5PW
9.1 - 10.2
-
-
13.8
-
gene product YGR010W
15
-
NMNAT-2
15.4
-
recombinant enzyme, purification step: Superose 12, with dATP as substrate
16.7
-
purification step: TSK phenyl-5PW
31
-
purification step: DEAE-Sephadex column, erythrocytes
51
-
recombinant enzyme, purification step: Superose 12, with NMN and ATP as substrate
74.2
-
recombinant enzyme YLR328W, purification step: phenyl Superose
76.5
-
recombinant enzyme, purification step: Superose 12, with nicotinic acid mononucleotide as substrate
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
5.6 - 8.5
-
6 - 9
-
plateau
6 - 9
-
erythrocytes
6 - 9
-
recombinant NMNAT-2
6.5 - 8
-
isoform yNMNAT-2
7 - 10
-
broad
7 - 8.5
-
isoform yNMNAT-1
7.4
-
-
7.4
activity assay; activity assay
7.5
assay at; assay at; assay at
7.5 - 8.5
-
YGR010W
7.8 - 8.4
-
YLR328W
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
3 - 11.3
-
-
6.5 - 8.5
-
-
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
30
-
assay at
37
-
assay at
37
-
assay at
37
assay at; assay at; assay at
37
activity assay; activity assay
55
-
erythrocytes
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
30 - 65
-
about half-maximal activity at 30C and about 60% of maximal activity at 65C, erythrocytes
37 - 97
-
activity shows a continuous increase up to 97C
37 - 97
activity shows a continuous increase up to 97C
pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
4.2
-
4.2, 4.9 and 6.2, isoform yNMNAT-1
4.7 - 4.8
-
isoelectric focusing, LKB 8100 ampholine column filled with a linear gradient of glycerol containing 1% ampholine carrier ampholytes
5
-
pH-electrophoretic mobility curve
5.4
-
FPLC chromatofocusing on a Mono P HR 5/5 column
5.5
-
isoelectric focusing with either pH 3-10 or pH 4-6 gradient, erythrocytes
5.6
-
predicted, isoform yNMNAT-2
6.2
-
isoelectric focusing
6.2
-
in the presence of 4 M urea, multiple pIs in its absence at pH 4.2, 4.9 and 6.2
6.59
theoretical value
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
Anser sp., Frog, Gadidae, Gallus gallus, pheasant
-
-
Manually annotated by BRENDA team
hNMNAT-2, whole cerebrum, cerebellum, occipital, frontal and temporal lobe cortices, and the putamen. The medulla shows minimal expression, none is detectable in the spinal cord
Manually annotated by BRENDA team
predominance of isozyme NMNAT1; predominance of isozyme NMNAT1; predominance of isozyme NMNAT1
Manually annotated by BRENDA team
-
enzyme is upregulated in the brain upon overexpression of poly-glutamine expanded protein
Manually annotated by BRENDA team
-
the enzyme localizes in photoreceptor and central brain synapses
Manually annotated by BRENDA team
-
Nmnat2 transcript is expressed predominately in the brain
Manually annotated by BRENDA team
-
highest expression
Manually annotated by BRENDA team
-
activity is high during mid luteal phase, when corpora lutea are mature with respect to structure and function, while it dramatically decreases following PGF2alpha analog injection
Manually annotated by BRENDA team
-
detected in the differentiated stomatal guard cells of the leaf surface
Manually annotated by BRENDA team
hepatocellular carcinoma cell line, predominance of isozyme NMNAT1, isozyme NMNAT3 is only present in liver and HepG2 cells; hepatocellular carcinoma cell line, predominance of isozyme NMNAT1, isozyme NMNAT3 is only present in liver and HepG2 cells; hepatocellular carcinoma cell line, predominance of isozyme NMNAT1, isozyme NMNAT3 is only present in liver and HepG2 cells
Manually annotated by BRENDA team
-
PNAT-3 weak
Manually annotated by BRENDA team
Mus musculus L1210
-
-
-
Manually annotated by BRENDA team
predominance of isozyme NMNAT1, isozyme NMNAT3 is only present in liver and HepG2 cells; predominance of isozyme NMNAT1, isozyme NMNAT3 is only present in liver and HepG2 cells; predominance of isozyme NMNAT1, isozyme NMNAT3 is only present in liver and HepG2 cells
Manually annotated by BRENDA team
-
low expression
Manually annotated by BRENDA team
-
primary cortical neuron
Manually annotated by BRENDA team
-
Tiazofurin resistant
Manually annotated by BRENDA team
hNMNAT-2 is markedly expressed in the islets of Langerhans. However, the pancreatic exocrine cells exhibit weak expression of hNMNAT-2 protein. Sections of pancreas from insulinoma patients show strong expression of hNMNAT-2 protein in the insulin-producing tumour cells, acinar cells exhibit low expression of hNMNAT-2 protein
Manually annotated by BRENDA team
-
PNAT-3 weak
Manually annotated by BRENDA team
-
differentiated
Manually annotated by BRENDA team
hepatocellular carcinoma cell line, isozyme NMNAT2 is found exclusively in SK-HEP1 cells; hepatocellular carcinoma cell line, isozyme NMNAT2 is found exclusively in SK-HEP1 cells; hepatocellular carcinoma cell line, isozyme NMNAT2 is found exclusively in SK-HEP1 cells
Manually annotated by BRENDA team
additional information
-
isoform Nmat2 is not detected in HEK-293T cells, NIH-3T3 cells, U-343 cells, U-87 cells, and primary glia cells
Manually annotated by BRENDA team
additional information
-
isoform Nmat2 is not detected in heart, spleen, lung, kidney, liver and skeletal muscle
Manually annotated by BRENDA team
additional information
-
not expressed in heart, bladder, kidney and liver
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
-
in HeLa cells, in addition to Golgi, isoform Nmnat2 localizes to Rab7-containing late endosomes
Manually annotated by BRENDA team
NMNAT2 is assocaiated with
Manually annotated by BRENDA team
isozyme NMNAT2; isozyme NMNAT2; isozyme NMNAT2; NMNAT2
Manually annotated by BRENDA team
-
isoform Nmnat2 is enriched in numerous membrane compartments including synaptic terminals
Manually annotated by BRENDA team
isozyme NMNAT3; isozyme NMNAT3; isozyme NMNAT3; NMNAT3
Manually annotated by BRENDA team
-
PNAT-1 and PNAT-2
Manually annotated by BRENDA team
-
chromatin-associated
Manually annotated by BRENDA team
NMNAT1 is exclusively located in
Manually annotated by BRENDA team
isozyme NMNAT1; isozyme NMNAT1; isozyme NMNAT1; Nmnat1
Manually annotated by BRENDA team
PDB
SCOP
CATH
ORGANISM
UNIPROT
Francisella tularensis subsp. tularensis (strain SCHU S4 / Schu 4)
Francisella tularensis subsp. tularensis (strain SCHU S4 / Schu 4)
Histophilus somni (strain 2336)
Histophilus somni (strain 2336)
Human immunodeficiency virus type 1 group M subtype B
Human immunodeficiency virus type 1 group M subtype B
Human immunodeficiency virus type 1 group M subtype B
Human immunodeficiency virus type 1 group M subtype B
Human immunodeficiency virus type 1 group M subtype B
Human immunodeficiency virus type 1 group M subtype B
Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H)
Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H)
Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H)
Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H)
Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H)
Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H)
Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H)
Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H)
Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H)
Synechocystis sp. (strain PCC 6803 / Kazusa)
Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
31900
-
calculated from nucleic acid sequence
642798
31900
-
-
642799
32700
for NMNAT3, SDS-PAGE
672248
33000
-
gel filtration, recombinant NMNAT-2 with a 6 x His-tag
642794
34400
-
calculated from nucleic acid sequence, NMNAT-2
642794
36000
for NMNAT1, SDS-PAGE
672248
37000
for NMNAT2, SDS-PAGE
672248
66000
-
gel filtration
642801, 642807, 642809
66000
-
-
661539
72000
-
gel filtration
642801, 642810
132000
-
gel filtration
642779
133000
-
gel filtration
642792
195000
-
gel filtration
642806
195000
-
-
642807
200000
-
gel filtration
642787
200000
-
gel filtration; sucrose density gradient centrifugation
642788, 642789
200000
-
gel filtration
642790
200000
-
gel filtration
642791
300000
-
gel filtration
642786
additional information
-
amino acid composition
642788
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
?
-
x * 21500, SDS-PAGE
?
-
x * 19600, SDS-PAGE
?
x * 36000, isozyme NMNAT1, SDS-PAGE, x * 37000, isozyme NMNAT2, SDS-PAGE, x * 32700, isozyme NMNAT3, SDS-PAGE; x * 36000, isozyme NMNAT1, SDS-PAGE, x * 37000, isozyme NMNAT2, SDS-PAGE, x * 32700, isozyme NMNAT3, SDS-PAGE; x * 36000, isozyme NMNAT1, SDS-PAGE, x * 37000, isozyme NMNAT2, SDS-PAGE, x * 32700, isozyme NMNAT3, SDS-PAGE
?
-
x * 24500, calculated: 24528
?
x * 34000, isoform NMAT2, SDS-PAGE
?
-
x * 34000, SDS-PAGE
?
-
x * 18600, SDS-PAGE
-
hexamer
-
alpha/beta-topology subunits, X-ray crystallography, barrel-like hexamer
hexamer
Methanothermobacter thermautotrophicum
-
x-ray crystallography
hexamer
Methanothermobacter thermautotrophicum
-
-
hexamer
syNadM-Nudix forms hexamer in both crystal and solution with two types of dimer interfaces, the dimer interface is formed primarily through ADPRase domain of each monomer, overview
homotetramer
4 * 34439, calculated
homotetramer
4 * 48000, calculated: 45859
monomer
-
1 * 36800, SDS-PAGE, recombinant NMNAT-2 with a 6 x His-tag
oligomer
Methanothermobacter thermautotrophicum
-
tetramer
-
alpha4, 4 * 33000, SDS-PAGE, with or without 2-mercaptoethanol
tetramer
-
alpha4, 4 * 33000, SDS-PAGE, no disulfide interchain bonds
tetramer
-
alpha4, 4 * 48000, SDS-PAGE, recombinant enzyme
tetramer
-
alpha4, 4 * 50000, SDS-PAGE, with or without 2-mercaptoethanol
tetramer
-
4 * 46000, isoform yNMNAT-2, SDS-PAGE; 4 * 48000, isoform yNMNAT-1, SDS-PAGE
additional information
bacterial NadM-Nudix is a bifunctional enzyme containing a nicotinamide mononucleotide adenylyltransferase and an ADP-ribose diphosphatase domain, structures of the N-terminal NadM domain and ADPR domain, overview
additional information
bacterial NadM-Nudix is a bifunctional enzyme containing a nicotinamide mononucleotide adenylyltransferase and an ADP-ribose diphosphatase domain, structures of the N-terminal NadM domain and ADPR domain, overview
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
palmitoylation
isoform NMAT2
palmitoylation
-
isoform Nmnat2 is palmitoylated in vitro (at Cys164 and Cys165) and this modification is required for membrane association
glycoprotein
-
2% carbohydrates; possibly ADP-ribosylated
glycoprotein
-
2% carbohydrates
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
X-ray diffraction structure determination and analysis at 2.3 A resolution, molecular replacement method, co-crystallization of ftNadM-Nudix complexed with the product AMP and Mn2+ ions in the Nudix active site
hanging drop vapor diffusion method, 20 mg/ml protein mixed with an equal volume of reservoir solution containing 1.8 M ammonium sulfate, 0.1 M Tris, pH 8 and 4% v/v isopropyl alcohol, 4-5 days at 20C, monoclinic space group P21 if 20% glycerol is used, hexagonal space group P6322 if light paraffin oil is used
-
hanging drop vapor diffusion method, 20 mg/ml protein mixed with an equal volume of reservoir solution containing 100 mM sodium cacodylate, pH 6-7, 200 mM Li2SO4 and 20-25% polyethylene glycol 400
-
hanging drop vapor diffusion method, 25 mg/ml protein in 100 mM HEPES, pH 7.2, 0.5 M NaCl, 2 mM dithiothreitol, 1 mM EDTA and 0.03% Brij-35 was incubated with 10 mM NAD+ and then mixed with an equal volume of reservoir solution containing 0.1 mM sodium acetate, pH 4.2-6.4 and 1.6-1.8 mM sodium formate at 20C for 1-2 weeks, monoclinic space group P21
-
crystals of the enzyme in complex with ATP are grown using the hanging-drop vapor-diffusion method. The structure in complex with ATP has been solved by X-ray crystallography at 2.0 A resolution, using a combination of single isomorphous replacement and density modification techniques. The structure reveals a hexamer with 32 point group symmetry composed of alpha/beta topology subunits
-
-
Methanothermobacter thermautotrophicum
-
hanging drop vapor diffusion method, in 100 mM HEPES, pH 7.5 at 20C, crystals of native enzyme were grown in 1.5 M Li2SO4, crystals of H19A enzyme were grown in 1.6 M Li2SO4, space group: P6322
Methanothermobacter thermautotrophicum
-
the enzyme is complexed with the co-purified NAD and pyrophosphate in the NadM-domain active site, and with ADPR substrate in the Nudix-domain, X-ray diffraction structure determination and analysis at 2.6 A resolution
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
3
-
inactivation below pH 3 is irreversible
642792
3.5 - 9.5
-
10 min stable at 4C
642784
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
4
-
10 min stable at pH 3.5-9.5
642784
55
-
5 min 54% loss of activity
642778
60
-
5 min 95% loss of activity
642778
60
-
5 min 39% loss of activity
642778
65
-
5 min 65% loss of activity
642778
70
-
stable for 6 h
642801, 642810
80
-
half life: 3 h
642801, 642810
80
stable at least 2 h
642810
85
half life: 75 min
642810
90
-
half life: 1 h
642801, 642810
90
half life: 30 min
642810
90
-
half-life is 1 h
661539
90
-
half-life is 30 min
661539
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
concentration procedures including lyophilization, dialysis against solid sucrose, membrane ultrafiltration lead to marked loss of activity
-
dithiothreitol and EDTA stabilize during purification
-
dithiothreitol stabalizes enzyme NMNAT-2
-
extremely labile in crude extracts, even in the presence of PMSF and sodium metabisulfite, EDTA or DTT does not stabilize
-
very sensitive to freeze-thawing, 50% glycerol stabilizes
-
bovine serum albumin stabilizes
-
chaotropic anions are strong destabilizers of the enzyme
-
dithiothreitol has no effect up to 50C but destabilizes the enzyme at higher temperatures
-
increasing ionic strenght of NaCl and KCl stabilizes the thermal stability of the enzyme
-
dithiothreitol stabilizes during purification
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, 0.5% v/v Triton X-100, 50 mM sodium phosphte buffer, pH 6.8, 0.5 M NaCl, 100% activity after 3 months
-
4C, 0.5% (v/v) Triton X-100, 50 mM sodium phosphate buffer, pH 6.8, 0.5 M NaCl, 80% activity after 3 months
-
4C, 0.3-0.5 mg/ml protein in 0.1-0.3 M NaCl, 1 mM DTT, several months
-
-20C, in stroma-free lysate: 30% loss of activity within 2-3 days, in washed erythrocytes: up to 1 month
-
-80C, 1 mM DTT, 0.5 mM EDTA, 2 weeks
-
-80C, NMNAT2 is stable
0-3C, E. coli enzyme: 31 days stable, yeast enzyme: 22% loss of activity within 31 days
-
4C, in 50 mM potassium phosphate buffer, pH 7.4, 1 mM DTT, 1 mM KCl, 1 mM MgCl2, 0.5 mM EDTA, 1 mM PMSF, several months
-
4C, NMNAT1 is stable
high KCl concentrations stabilize during storage
-
-20C, at pH 7.5 in buffer containing both glycerol and TCEP, 6 months, about 20% loss of activity
-20C, at pH 7.5 in buffer containing both glycerol and TCEP, 6 months, about 55% loss of activity
-20C, at pH 7.5 in buffer containing both glycerol and TCEP, 6 months, about 60% loss of activity
4C, at pH 7.5 in buffer containing both glycerol and TCEP, 6 months, about 55% loss of activity
4C, at pH 7.5 in buffer containing both glycerol and TCEP, 6 months, about 60% loss of activity
4C, at pH 7.5 in buffer containing both glycerol and TCEP, 6 months, about 90% loss of activity
-20C, in 20 mM HEPES buffer, pH 7.5, about 20% loss of activity within 1 month
-
4C, in 20 mM HEPES buffer, pH 7.5, about 30% loss of activity within 2 weeks
-
-20C, several weeks without loss in activity
-
-15C, several months
-
-27C, several months
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partial
-
erythrocytes, partial
-
; NMNAT1; NMNAT2
Ni-NTA agarose bead chromatography
-
partial
-
recombinant enzyme NMNAT-2
-
recombinant His-tagged isozymes NMNAT1, NMNAT2, and NMNAT3 from Escherichia coli strain BL21(DE3) by nickel or TALON affinity chromatography; recombinant His-tagged isozymes NMNAT1, NMNAT2, and NMNAT3 from Escherichia coli strain BL21(DE3) by nickel or TALON affinity chromatography; recombinant His-tagged isozymes NMNAT1, NMNAT2, and NMNAT3 from Escherichia coli strain BL21(DE3) by nickel or TALON affinity chromatography
-
Methanothermobacter thermautotrophicum
-
Ni-NTA affinity chromatography; Talon affinity column chromatography; Talon affinity column chromatography
Ni-NTA agarose bead chromatography
-
prepared from cerebrum for activity assays; prepared from cerebrum for activity assays
partial
pheasant
-
FPLC reveals microheterogeneity, possibly through poly-ADP-ribosylation of the enzyme
-
recombinant enzyme
-
partial
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
; NMNAT1; NMNAT2
expressed in Escherichia coli Rosetta (DE3)pLysS cells
-
expression in Escherichia coli BL21
-
expression in Escherichia coli BL21 with a 6 x His-tag
-
expression in Escherichia coli, NMNAT-2 with a 6 x His-tag; overexpression in HeLa and HEK293 cells as GFP-fusion protein
-
His-tagged isozymes NMNAT1, NMNAT2, and NMNAT3, expression in Escherichia coli strain BL21(DE3); His-tagged isozymes NMNAT1, NMNAT2, and NMNAT3, expression in Escherichia coli strain BL21(DE3); His-tagged isozymes NMNAT1, NMNAT2, and NMNAT3, expression in Escherichia coli strain BL21(DE3)
overexpression in Escherichia coli of PNAT-3 with a His-tag
-
expression in Escherichia coli BL21
-
expression in Escherichia coli BL21
Methanothermobacter thermautotrophicum
-
expressed in Escherichia coli BL21(DE3) cells; expressed in Escherichia coli BL21(DE3) cells; expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli Rosetta (DE3)pLysS cells
-
transgenic mice overexpressing NMNAT1 and the mutant WldS(W258A) are generated; transgenic mice overexpressing NMNAT3 are generated
expression in Escherichia coli BL21
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
under hypoxia, hypoxia-inducible factor 1alpha up-regulates the enzyme indirectly through the induction of heat shock factor. Acute exposure to hypoxia (5% O2) for 3 h results in a 3.1fold up-regulation in enzyme transcript level. Heat shock at 37C induces a 3fold increase in protein level between 8 and 12 h post-heat shock and a 3fold increase in enzyme transcript level at 3 h post-heat shock. A 3.3fold increase of protein level is seen in flies fed with 20 mM paraquat for 72 h
-
isoform Nmnat2 protein levels increase dramatically upon differentiation of SH-SY5Y cells
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
S136D
-
overexpession
W169A
-
catalytically inactive
C164A/C165A
the isoform NMAT2 mutant is consistently more strongly expressed than the wild type protein
C164S/C165S
-
the mutant protein is not palmitoylated (soluble), appears diffuse in the cytoplasm and does not colocalize with GM130. However, the mutant is fully active and shows even higher kcat values compared to the wild type enzyme
H24A
protein expressed from the mutant construct is greatly reduced in activity compared with the wild-type protein
S136D
-
overexpession
W169A
-
catalytically inactive
W92G
protein expressed from the mutant construct is greatly reduced in activity compared with the wild-type protein
H16A
Methanothermobacter thermautotrophicum
-
slower turnover and higher KM-value for ATP then wild-type
H19A
Methanothermobacter thermautotrophicum
-
loss of activity
H19A
Methanothermobacter thermautotrophicum
-
slower turnover but lower KM-value for ATP then wild-type
R11A
Methanothermobacter thermautotrophicum
-
slower turnover and higher KM-value for ATP then wild-type
R11K
Methanothermobacter thermautotrophicum
-
slower turnover and higher KM-value for ATP then wild-type
R136A
Methanothermobacter thermautotrophicum
-
slower turnover and higher KM-value for ATP then wild-type
R136K
Methanothermobacter thermautotrophicum
-
slower turnover and higher KM-value for ATP then wild-type
WldS(W258A)
the chimeric WldS protein is composed of the N-terminal 70 aa of Ufd2a/Ube4b, an ubiquitin-chain assembly factor, fused to the complete sequence of NMNAT1, the W258A mutation disrupts the NAD-synthesizing activity
additional information
-
overexpression of NAD synthase nicotinamide mononucleotide adenylyltransferase protects against spinocerebellar ataxia-induced neurodegeneration
C164S/C165S
-
the mutant protein is not palmitoylated (soluble), appears diffuse in the cytoplasm and does not colocalize with GM130. However, the mutant is fully active and shows even higher kcat values compared to the wild type enzyme
additional information
naturally occuring mutant mice, wldS, showing delayed Wallerian degeneration phenotype, suggest that axonal degeneration is an active process, the wldS mutation comprises an 85 kb tandem triplicon, which causes overexpression of the chimeric WldS protein composed of the N-terminal 70 aa of Ufd2a/Ube4b, an ubiquitin-chain assembly factor, fused to the complete sequence of NMNAT1
APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
drug development
development of NMNATase inhibitors as potential therapeutics against tularemia
medicine
-
given its vital role in cell life, the enzyme represents a possible target for the development of new antibacterial agents
medicine
-
given its vital role in cell life, the enzyme represents a possible target for the development of new antibacterial agents
-
medicine
the mitochondrial localization of NMNAT activity plays an important role in NMNAT expression-mediated axonal protection
additional information
The expression of Nmnat1 itself is sufficient to protect axons in an in vitro Wallerian degeneration assay, suggesting that increased NAD production is crucial for axonal protection.; The expression of Nmnat1 itself is sufficient to protect axons in an in vitro Wallerian degeneration assay, suggesting that increased NAD production is crucial for axonal protection.