Information on EC 2.7.7.1 - nicotinamide-nucleotide adenylyltransferase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea

EC NUMBER
COMMENTARY hide
2.7.7.1
-
RECOMMENDED NAME
GeneOntology No.
nicotinamide-nucleotide adenylyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + nicotinamide ribonucleotide = diphosphate + NAD+
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
condensation
nucleotidyl group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Metabolic pathways
-
-
NAD biosynthesis III
-
-
NAD metabolism
-
-
NAD salvage pathway IV
-
-
Nicotinate and nicotinamide metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP:nicotinamide-nucleotide adenylyltransferase
Nicotinate nucleotide can also act as acceptor. See also EC 2.7.7.18 nicotinate-nucleotide adenylyltransferase.
CAS REGISTRY NUMBER
COMMENTARY hide
9032-70-6
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Frog
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
skipjack tuna
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Methanothermobacter thermautotrophicum
L1210
-
-
Manually annotated by BRENDA team
pheasant
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + 3-acetylpyridine-NAD+
?
show the reaction diagram
ATP + 3-pyridinealdehyde-NAD+
?
show the reaction diagram
ATP + beta-NMNH
diphosphate + ?
show the reaction diagram
-
-
-
-
r
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
ATP + nicotinate D-ribonucleotide
diphosphate + NAD+
show the reaction diagram
-
i.e. nicotinic acid adenine dinucleotide
-
?
ATP + nicotinate mononucleotide
diphosphate + nicotinate adenine dinucleotide
show the reaction diagram
-
-
-
-
?
ATP + nicotinate ribonucleotide
diphosphate + nicotinic acid-adenine dinucleotide
show the reaction diagram
ATP + nicotinic acid mononucleotide
diphosphate + deamido-NAD+
show the reaction diagram
-
-
-
r
ATP + nicotinic acid mononucleotide
diphosphate + nicotinic acid adenine dinucleotide
show the reaction diagram
ATP + NMN
diphosphate + NAD+
show the reaction diagram
ATP + NMNH
diphosphate + NADH
show the reaction diagram
-
-
-
?
ATP + tiazofurin 5'-monophosphate
?
show the reaction diagram
i.e. TrMP, substrate od isozymes NMNAT1 and NMNAT3, but not of isozyme NMNAT2
-
-
?
ATP + tiazofurin monophosphate
?
show the reaction diagram
i.e. TrMP, substrate od isozymes NMNAT1 and NMNAT3, but not of isozyme NMNAT2
-
-
?
CTP + nicotinamide ribonucleotide
diphosphate + nicotinamide cytosine dinucleotide
show the reaction diagram
-
isoform yNMNAT-2
-
-
?
dATP + nicotinamide ribonucleotide
diphosphate + ?
show the reaction diagram
-
isoform yNMNAT-1
-
-
?
dATP + nicotinamide ribonucleotide
diphosphate + nicotinamide deoxyadenosine dinucleotide
show the reaction diagram
-
isoform yNMNAT-2
-
-
?
deoxy-ATP + nicotinamide ribonucleotide
?
show the reaction diagram
deoxy-ATP + nicotinate ribonucleotide
?
show the reaction diagram
GTP + nicotinamide ribonucleotide
?
show the reaction diagram
-
-
-
?
GTP + nicotinamide ribonucleotide
diphosphate + NGD+
show the reaction diagram
GTP + nicotinamide ribonucleotide
diphosphate + nicotinamide guanine dinucleotide
show the reaction diagram
-
isoform yNMNAT-2
-
-
?
GTP + nicotinamide ribonucleotide
nicotineamide guanine dinucleotide + diphosphate
show the reaction diagram
only isoenzyme NMNAT3
-
-
?
GTP + NMN
diphosphate + NGD+
show the reaction diagram
substrates of isozyme NMNAT3
i.e. nicotinamide guanosine dinucleotide
-
?
ITP + nicotinamide ribonucleotide
?
show the reaction diagram
-
-
-
?
ITP + nicotinamide ribonucleotide
diphosphate + nicotinamide hypoxanthine dinucleotide
show the reaction diagram
ITP + nicotinamide ribonucleotide
nicotinamide hypoxanthine dinucleotide + diphosphate
show the reaction diagram
only isoenzyme NMNAT3
-
-
?
ITP + NMN
diphosphate + inosine nicotinamide dinucleotide
show the reaction diagram
substrates of isozyme NMNAT3
i.e. nicotinamide hypoxanthine dinucleotide
-
?
NADH + diphosphate
?
show the reaction diagram
nicotinamide hypoxanthine dinucleotide + diphosphate
?
show the reaction diagram
98% of the activity with NAD+, NMNAT3
-
-
?
nicotinamide hypoxanthine dinucleotide + diphosphate
ITP + nicotinamide ribonucleotide
show the reaction diagram
nicotinate mononucleotide + ATP
nicotinate adenine dinucleotide + diphosphate
show the reaction diagram
nicotinic acid adenine dinucleotide phosphate + diphosphate
?
show the reaction diagram
nicotinic acid mononucleotide + ATP
nicotinic acid adenine dinucleotide + diphosphate
show the reaction diagram
NMN + ATP
NAD+ + diphosphate
show the reaction diagram
-
-
-
?
tiazofurin + ATP
tiazofurin adenine dinucleotide + diphosphate
show the reaction diagram
tiazofurin monophosphate + ATP
tiazofurin adenine dinucleotide + diphosphate
show the reaction diagram
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + nicotinamide ribonucleotide
diphosphate + NAD+
show the reaction diagram
ATP + NMN
diphosphate + NAD+
show the reaction diagram
GTP + nicotinamide ribonucleotide
nicotineamide guanine dinucleotide + diphosphate
show the reaction diagram
Q96T66, Q9BZQ4, Q9HAN9
only isoenzyme NMNAT3
-
-
?
ITP + nicotinamide ribonucleotide
nicotinamide hypoxanthine dinucleotide + diphosphate
show the reaction diagram
Q96T66, Q9BZQ4, Q9HAN9
only isoenzyme NMNAT3
-
-
?
nicotinate mononucleotide + ATP
nicotinate adenine dinucleotide + diphosphate
show the reaction diagram
F4K687
PreissHandler-dependent pathway
-
-
?
nicotinic acid mononucleotide + ATP
nicotinic acid adenine dinucleotide + diphosphate
show the reaction diagram
NMN + ATP
NAD+ + diphosphate
show the reaction diagram
Q96T66, Q9BZQ4, Q9HAN9
-
-
-
?
tiazofurin monophosphate + ATP
tiazofurin adenine dinucleotide + diphosphate
show the reaction diagram
Q96T66, Q9BZQ4, Q9HAN9
-
-
-
?
additional information
?
-
-
NAD synthase nicotinamide mononucleotide adenylyltransferase is a stress-response protein that acts as a chaperone for neuronal maintenance and protection
-
-
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Divalent cations
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5,5'-dithiobis(2-nitrobenzoic acid)
-
-
ADP
NMNAT1; NMNAT2
ADP-ribose
-
-
ADPribose
Ag2+
-
IC50: 0.037 mM
beta-naphthoquinone 4-sulfonate
-
strong
beta-NMNH
-
-
diphosphate
Fe3+
-
-
gallotannin
Metal ions
-
strong, Mg2+, Ni2+ or Co2+ activates
-
N-bromosuccinimide
-
strong
NaAD+
i.e. nicotinic acid adenine dinucleotide, product inhibition, competitive for isozyme NMNAT3, and mixed-type for isozymes NMNAT1 and NMNAT2 NMN, and vice versa against ATP; i.e. nicotinic acid adenine dinucleotide, product inhibition, competitive for isozyme NMNAT3, and mixed-type for isozymes NMNAT1 and NMNAT2 NMN, and vice versa against ATP; i.e. nicotinic acid adenine dinucleotide, product inhibition, competitive for isozyme NMNAT3, and mixed-type for isozymes NMNAT1 and NMNAT2 NMN, and vice versa against ATP
NaCl
-
reverse reaction
NADP+
NMNAT1; NMNAT2
nicotinamide ribonucleotide
-
strong, nicotinate ribonucleotide as substrate
nicotinate ribonucleotide
-
nicotinamide ribonucleotide as substrate
nicotinic acid adenine dinucleotide
product inhibition
p-chloromercuribenzoate
-
-
P1-(adenosine-5')-P3-(nicotinamide ribose-5')triphosphate
P1-(adenosine-5')-P3-(nicotinamide-riboside-5')-triphosphate
-
P1-(adenosine-5')-P4-(nicotinamide ribose-5')tetraphosphate
P1-(adenosine-5')-P4-(nicotinamide-riboside-5')-tetraphosphate
-
P1-(adenosine-5')-P4-(nicotinic-acid-riboside-5')-tetraphosphate
-
P1-(nicotinamide-riboside-5')-P3-(adenosine-5')-triphosphate
i.e. Np3AD; i.e. Np3AD; i.e. Np3AD
P1-(nicotinamide-riboside-5')-P4-(adenosine-5')-tetraphosphate
i.e. Np4AD; i.e. Np4AD; i.e. Np4AD
P1-(nicotinate-riboside-5')-P4-(adenosine-5')-tetraphosphate
i.e. Nap4AD; i.e. Nap4AD; i.e. Nap4AD
additional information
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
dithiothreitol
-
stimulates the catalytic activity of NMNAT-2 up to 30%
epigallocatechin gallate
0.05 mM, 10% activation, NMNAT1; 0.05 mM, 1.4fold activation, NMNAT3; 0.05 mM, 2.28fold activation, NMNAT2
NH4Cl
-
activation, can substitute for KCl
additional information
-
SO42- has no effect
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.27
3-acetylpyridine-NAD+
-
37C, pH 7.5
0.74
3-pyridinealdehyde-NAD+
-
37C, pH 7.5
0.041
ADP-ribose
pH 8.2, 37C
0.0008 - 1.4
ATP
0.625
beta-NMNH
-
37C, pH 7.4
0.45
dATP
-
37C
0.0045 - 0.029
deamido-NAD+
0.083 - 5
diphosphate
0.276
GTP
NMNAT3
0.023 - 0.37
NAD+
0.021 - 0.7
nicotinamide mononucleotide
0.00295 - 0.4
nicotinamide ribonucleotide
0.08 - 5
nicotinate ribonucleotide
0.0145 - 0.15
nicotinic acid mononucleotide
0.91
nicotinic acid-adenine dinucleotide
-
37C, pH 7.4
0.000019 - 0.594
NMN
0.13 - 0.304
NMNH
0.0304
reduced nicotinamide mononucleotide
-
0.055
tiazofurin
-
YGR010W
0.37 - 100
tiazofurin monophosphate
2.1 - 100
tiazofurin riboside 5'-monophosphate
additional information
additional information
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3.6
ADP-ribose
Francisella tularensis
Q5NHR1
pH 8.2, 37C
0.0000333 - 53.9
ATP
0.654 - 15.5
nicotinamide ribonucleotide
3.8 - 42.9
nicotinic acid mononucleotide
2.5 - 53.8
NMN
3.3 - 18.6
NMNH
0.42 - 14.9
tiazofurin riboside 5'-monophosphate
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
20 - 450
ATP
10 - 610
nicotinamide ribonucleotide
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.16 - 1
diphosphate
0.023 - 0.502
NaAD+
0.067 - 1.418
NAD+
0.0315 - 0.089
P1-(adenosine-5')-P3-(nicotinamide-riboside-5')-triphosphate
0.0328 - 0.3283
P1-(adenosine-5')-P3-(nicotinic-acid-riboside-5')-triphosphate
0.0242 - 0.0736
P1-(adenosine-5')-P4-(nicotinamide-riboside-5')-tetraphosphate
0.0217 - 0.6576
P1-(adenosine-5')-P4-(nicotinic-acid-riboside-5')-tetraphosphate
0.0315 - 0.089
P1-(nicotinamide-riboside-5')-P3-(adenosine-5')-triphosphate
0.0242 - 0.0736
P1-(nicotinamide-riboside-5')-P4-(adenosine-5')-tetraphosphate
0.0217 - 0.6576
P1-(nicotinate-riboside-5')-P4-(adenosine-5')-tetraphosphate
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.037
Ag2+
Bos taurus
-
IC50: 0.037 mM
0.2
Cd2+
Bos taurus
-
IC50: 0.2 mM
0.1
Cr3+
Bos taurus
-
IC50: 0.1 mM
0.025
Cu2+
Bos taurus
-
IC50: 0.025 mM
0.002 - 0.055
gallotannin
0.017
Hg2+
Bos taurus
-
IC50: 0.017 mM
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0038
-
NAD+-synthesis
0.004
-
NAD+ as substrate
0.024
-
deamido-NAD+ as substrate
0.068
-
deamido-NAD+-synthesis
0.11
-
immobilized enzyme, reverse reaction
0.112
-
tiazofurin as substrate, YGR010W
2.8
-
purification step: Matrix Gel Green A
3.3
-
NAD+-synthesis
3.85
-
deamido-NAD+ as substrate
5
-
NAD+ as substrate, yeast
7.3
-
deamido-NAD+-synthesis
9.1 - 10.2
-
-
13.8
-
gene product YGR010W
15
-
NMNAT-2
15.4
-
recombinant enzyme, purification step: Superose 12, with dATP as substrate
16.7
-
purification step: TSK phenyl-5PW
31
-
purification step: DEAE-Sephadex column, erythrocytes
51
-
recombinant enzyme, purification step: Superose 12, with NMN and ATP as substrate
76.5
-
recombinant enzyme, purification step: Superose 12, with nicotinic acid mononucleotide as substrate
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.6 - 8.5
-
6.5 - 8
-
isoform yNMNAT-2
7 - 10
-
broad
7 - 8.5
-
isoform yNMNAT-1
7.5
assay at; assay at; assay at
7.5 - 8.5
-
YGR010W
7.8 - 8.4
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3 - 11.3
-
-
6.5 - 8.5
-
-
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
-
assay at
55
-
erythrocytes
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30 - 65
-
about half-maximal activity at 30C and about 60% of maximal activity at 65C, erythrocytes
37 - 97
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.7 - 4.8
-
isoelectric focusing, LKB 8100 ampholine column filled with a linear gradient of glycerol containing 1% ampholine carrier ampholytes
5
-
pH-electrophoretic mobility curve
5.5
-
isoelectric focusing with either pH 3-10 or pH 4-6 gradient, erythrocytes
5.6
-
predicted, isoform yNMNAT-2
6.59
theoretical value
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
activity is high during mid luteal phase, when corpora lutea are mature with respect to structure and function, while it dramatically decreases following PGF2alpha analog injection
Manually annotated by BRENDA team
-
detected in the differentiated stomatal guard cells of the leaf surface
Manually annotated by BRENDA team
hepatocellular carcinoma cell line, predominance of isozyme NMNAT1, isozyme NMNAT3 is only present in liver and HepG2 cells; hepatocellular carcinoma cell line, predominance of isozyme NMNAT1, isozyme NMNAT3 is only present in liver and HepG2 cells; hepatocellular carcinoma cell line, predominance of isozyme NMNAT1, isozyme NMNAT3 is only present in liver and HepG2 cells
Manually annotated by BRENDA team
-
Tiazofurin resistant
Manually annotated by BRENDA team
-
differentiated
Manually annotated by BRENDA team
hepatocellular carcinoma cell line, isozyme NMNAT2 is found exclusively in SK-HEP1 cells; hepatocellular carcinoma cell line, isozyme NMNAT2 is found exclusively in SK-HEP1 cells; hepatocellular carcinoma cell line, isozyme NMNAT2 is found exclusively in SK-HEP1 cells
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
in HeLa cells, in addition to Golgi, isoform Nmnat2 localizes to Rab7-containing late endosomes
Manually annotated by BRENDA team
PDB
SCOP
CATH
ORGANISM
UNIPROT
Francisella tularensis subsp. tularensis (strain SCHU S4 / Schu 4)
Francisella tularensis subsp. tularensis (strain SCHU S4 / Schu 4)
Histophilus somni (strain 2336)
Histophilus somni (strain 2336)