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Literature summary for 2.7.7.1 extracted from
- Subcellular compartmentation and differential catalytic properties of the three human nicotinamide mononucleotide adenylyltransferase isoforms (2005), J. Biol. Chem., 280, 36334-36341.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| epigallocatechin gallate | 0.05 mM, 1.4fold activation, NMNAT3 | Homo sapiens |  |
| epigallocatechin gallate | 0.05 mM, 10% activation, NMNAT1 | Homo sapiens | |
| epigallocatechin gallate | 0.05 mM, 2.28fold activation, NMNAT2 | Homo sapiens | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Homo sapiens |
| NMNAT1 | Homo sapiens |
| NMNAT2 | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | NMNAT1; NMNAT2 | Homo sapiens | |
| AMP | NMNAT2 | Homo sapiens | |
| gallotannin | IC50: 0.002 mM, NMNAT3; IC50: 0.01 mM; IC50: 0.055 mM, NMNAT2 | Homo sapiens | |
| NADP+ | NMNAT1; NMNAT2 | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.029 | - |
ATP | NMNAT3 | Homo sapiens | |
| 0.032 | - |
nicotinamide ribonucleotide | NMNAT2 | Homo sapiens | |
| 0.034 | - |
nicotinamide ribonucleotide | NMNAT1 | Homo sapiens | |
| 0.04 | - |
ATP | NMNAT1 | Homo sapiens | |
| 0.059 | - |
NAD+ | NMNAT1 | Homo sapiens | |
| 0.07 | - |
NAD+ | NMNAT2 | Homo sapiens | |
| 0.13 | - |
NAD+ | NMNAT3 | Homo sapiens | |
| 0.204 | - |
ATP | NMNAT2 | Homo sapiens | |
| 0.209 | - |
nicotinamide ribonucleotide | NMNAT3 | Homo sapiens | |
| 0.39 | - |
diphosphate | NMNAT3 | Homo sapiens | |
| 0.937 | - |
diphosphate | NMNAT1 | Homo sapiens | |
| 1.119 | - |
diphosphate | NMNAT2 | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| Golgi apparatus | NMNAT2 is assocaiated with | Homo sapiens | 5794 | - |
| mitochondrion | NMNAT3 | Homo sapiens | 5739 | - |
| nucleus | NMNAT1 is exclusively located in | Homo sapiens | 5634 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + nicotinamide ribonucleotide | Homo sapiens | central enzyme of NAD biosynthetic pathway | diphosphate + NAD+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | Q96T66 | isoform NMNAT3 | - |
| Homo sapiens | Q9BZQ4 | isoform NMNAT2 | - |
| Homo sapiens | Q9HAN9 | isoform NMNAT1 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
| NMNAT1 | Homo sapiens |
| NMNAT2 | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | NMNAT2 | Homo sapiens | - |
| heart | NMNAT1 | Homo sapiens | - |
| kidney | NMNAT1 | Homo sapiens | - |
| lung | NMNAT3 | Homo sapiens | - |
| skeletal muscle | NMNAT1 | Homo sapiens | - |
| spleen | NMNAT3 | Homo sapiens | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, NMNAT2 is stable | Homo sapiens |
| 4°C, NMNAT1 is stable | Homo sapiens |
| 4°C, stable | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + nicotinamide ribonucleotide | central enzyme of NAD biosynthetic pathway | Homo sapiens | diphosphate + NAD+ | - |
? | |
| ATP + nicotinamide ribonucleotide | NMNAT1 | Homo sapiens | diphosphate + NAD+ | - |
r | |
| ATP + nicotinamide ribonucleotide | NMNAT2 | Homo sapiens | diphosphate + NAD+ | - |
r | |
| ATP + nicotinamide ribonucleotide | NMNAT3 | Homo sapiens | diphosphate + NAD+ | - |
r | |
| GTP + nicotinamide ribonucleotide | 19% of the activity with GTP, reverse reaction with 57% of the activity with NAD+, NMNA3 | Homo sapiens | diphosphate + NGD+ | - |
r | |
| GTP + nicotinamide ribonucleotide | 3% of the activity with GTP, reverse reaction with 5% of the activity with NAD+, NMNAT2 | Homo sapiens | diphosphate + NGD+ | - |
r | |
| GTP + nicotinamide ribonucleotide | 7% of the activity with GTP, reverse reaction with 26% of the activity with NAD+, NMNAT1 | Homo sapiens | diphosphate + NGD+ | - |
r | |
| ITP + nicotinamide ribonucleotide | 27% of the activity with ATP, NMNAT3 | Homo sapiens | diphosphate + nicotinamide hypoxanthine dinucleotide | - |
? | |
| ITP + nicotinamide ribonucleotide | 5% of the activity with ATP, NMNAT2 | Homo sapiens | diphosphate + nicotinamide hypoxanthine dinucleotide | - |
? | |
| ITP + nicotinamide ribonucleotide | 6% of the activity with ATP, NMNAT1 | Homo sapiens | diphosphate + nicotinamide hypoxanthine dinucleotide | - |
? | |
| NADH + diphosphate | 53% of the activity with NAD+, NMNAT1 | Homo sapiens | ? | - |
? | |
| NADH + diphosphate | 98% of the activity with NAD+, NMNAT2 | Homo sapiens | ? | - |
? | |
| NADH + diphosphate | 99% of the activity with NAD+, NMNAT3 | Homo sapiens | ? | - |
? | |
| nicotinamide hypoxanthine dinucleotide + diphosphate | 98% of the activity with NAD+, NMNAT3 | Homo sapiens | ? | - |
? | |
| nicotinamide hypoxanthine dinucleotide + diphosphate | 37% of the activity with NAD+, NMNAT2 | Homo sapiens | ITP + nicotinamide ribonucleotide | - |
? | |
| nicotinamide hypoxanthine dinucleotide + diphosphate | 55% of the activity with NAD+, NMNAT1 | Homo sapiens | ITP + nicotinamide ribonucleotide | - |
? | |
| nicotinic acid adenine dinucleotide phosphate + diphosphate | 93% of the activity with NAD+, NMNAT1 | Homo sapiens | ? | - |
? | |
| nicotinic acid adenine dinucleotide phosphate + diphosphate | 98% of the activity with NAD+, NMNAT2 | Homo sapiens | ? | - |
? | |
| nicotinic acid adenine dinucleotide phosphate + diphosphate | as active as with NAD+, NMNAT3 | Homo sapiens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| nicotinamide mononucleotide adenylyltransferase | - |
Homo sapiens |
| NMNAT1 | - |
Homo sapiens |
| NMNAT2 | - |
Homo sapiens |
| NMNAT3 | - |
Homo sapiens |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.002 | - |
IC50: 0.002 mM, NMNAT3 | Homo sapiens | gallotannin | |
| 0.01 | - |
IC50: 0.01 mM | Homo sapiens | gallotannin | |
| 0.055 | - |
IC50: 0.055 mM, NMNAT2 | Homo sapiens | gallotannin |
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