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Literature summary for 2.7.7.1 extracted from

  • Balducci, E.; Orsomando, G.; Polzonetti, V.; Vita, A.; Emanuelli, M.; Raffaelli, N.; Ruggieri, S.; Magni, G.; Natalini, P.
    NMN adenylyltransferase from bull testis: purification and properties (1995), Biochem. J., 310, 395-400.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.1

Inhibitors

Inhibitors Comment Organism Structure
Ag2+ IC50: 0.037 mM Bos taurus
Cd2+ IC50: 0.2 mM Bos taurus
Cr3+ IC50: 0.1 mM Bos taurus
Cu2+ IC50: 0.025 mM Bos taurus
Hg2+ IC50: 0.017 mM Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.023
-
 ATP 37°C, pH 7.4 Bos taurus
0.11
-
 NMN 37°C, pH 7.4 Bos taurus
0.15
-
 nicotinic acid mononucleotide 37°C, pH 7.4 Bos taurus
0.167
-
 diphosphate 37°C, pH 7.4 Bos taurus
0.37
-
 NAD+ 37°C, pH 7.4 Bos taurus
0.625
-
 beta-NMNH 37°C, pH 7.4 Bos taurus
0.91
-
 nicotinic acid-adenine dinucleotide 37°C, pH 7.4 Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
 alpha4, 4 * 33000, SDS-PAGE, no disulfide interchain bonds Bos taurus
133000
-
 gel filtration Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + nicotinamide ribonucleotide Bos taurus
-
 diphosphate + NAD+
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
testis
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
16.7
-
 purification step: TSK phenyl-5PW Bos taurus

Storage Stability

Storage Stability Organism
-20°C, 0.5% v/v Triton X-100, 50 mM sodium phosphte buffer, pH 6.8, 0.5 M NaCl, 100% activity after 3 months Bos taurus
4°C, 0.5% (v/v) Triton X-100, 50 mM sodium phosphate buffer, pH 6.8, 0.5 M NaCl, 80% activity after 3 months Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + beta-NMNH
-
 Bos taurus diphosphate + ?
-
 r
ATP + nicotinamide ribonucleotide
-
 Bos taurus diphosphate + NAD+
-
 ?
ATP + nicotinamide ribonucleotide i.e. NMN or nicotinamide mononucleotide Bos taurus diphosphate + NAD+
-
 r
ATP + nicotinate ribonucleotide i.e. nicotinate mononucleotide Bos taurus diphosphate + nicotinic acid-adenine dinucleotide
-
 r

Subunits

Subunits Comment Organism
tetramer alpha4, 4 * 33000, SDS-PAGE, no disulfide interchain bonds Bos taurus

pH Range

pH Minimum pH Maximum Comment Organism
3 11.3
-
 Bos taurus

pH Stability

pH Stability pH Stability Maximum Comment Organism
3
-
 inactivation below pH 3 is irreversible Bos taurus

pI Value

Organism Comment pI Value Maximum pI Value
Bos taurus isoelectric focusing
-
 6.2

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.017
-
 IC50: 0.017 mM Bos taurus Hg2+
0.025
-
 IC50: 0.025 mM Bos taurus Cu2+
0.037
-
 IC50: 0.037 mM Bos taurus Ag2+
0.1
-
 IC50: 0.1 mM Bos taurus Cr3+
0.2
-
 IC50: 0.2 mM Bos taurus Cd2+