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Literature summary for 2.7.7.1 extracted from

  • Emanuelli, M.; Carnevali, F.; Saccucci, F.; Pierella, F.; Amici, A.; Raffaelli, N.; Magni, G.
    Molecular cloning, chromosomal localization, tissue mRNA levels, bacterial expression, and enzymatic properties of human NMN adenylyltransferase (2001), J. Biol. Chem., 276, 406-412.
    View publication on PubMed
Search for more literature for 2.7.7.1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.023
-
 NMN 37°C Homo sapiens
0.036
-
 ATP 37°C Homo sapiens
0.116
-
 nicotinic acid mononucleotide 37°C Homo sapiens
0.45
-
 dATP 37°C Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+
-
 Homo sapiens
Mg2+
-
 Homo sapiens
Mn2+
-
 Homo sapiens
Ni2+
-
 Homo sapiens
Zn2+
-
 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31900
-
 calculated from nucleic acid sequence Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + nicotinamide ribonucleotide Homo sapiens
-
 diphosphate + NAD+
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9HAN9
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
placenta
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
15.4
-
 recombinant enzyme, purification step: Superose 12, with dATP as substrate Homo sapiens
51
-
 recombinant enzyme, purification step: Superose 12, with NMN and ATP as substrate Homo sapiens
76.5
-
 recombinant enzyme, purification step: Superose 12, with nicotinic acid mononucleotide as substrate Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + nicotinamide ribonucleotide
-
 Homo sapiens diphosphate + NAD+
-
 ?
ATP + nicotinamide ribonucleotide i.e. NMN or nicotinamide mononucleotide Homo sapiens diphosphate + NAD+
-
 r
ATP + nicotinate ribonucleotide i.e. nicotinate mononucleotide Homo sapiens diphosphate + nicotinic acid-adenine dinucleotide
-
 r
deoxy-ATP + nicotinamide ribonucleotide
-
 Homo sapiens ?
-
 ?