Information on EC 2.7.1.33 - pantothenate kinase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea

EC NUMBER
COMMENTARY hide
2.7.1.33
-
RECOMMENDED NAME
GeneOntology No.
pantothenate kinase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + (R)-pantothenate = ADP + (R)-4'-phosphopantothenate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
coenzyme A biosynthesis II (mammalian)
-
-
coenzyme A metabolism
-
-
Metabolic pathways
-
-
Pantothenate and CoA biosynthesis
-
-
phosphopantothenate biosynthesis I
-
-
phosphopantothenate biosynthesis II
-
-
SYSTEMATIC NAME
IUBMB Comments
ATP:(R)-pantothenate 4'-phosphotransferase
-
CAS REGISTRY NUMBER
COMMENTARY hide
9026-48-6
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
ecotype Colombia-0
-
-
Manually annotated by BRENDA team
gene coaX
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
no activity in Lactobacillus helveticus
-
-
-
Manually annotated by BRENDA team
no activity in Neurospora crassa
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Rattus norvegicus Sprague-Dawley
Sprague-Dawley
-
-
Manually annotated by BRENDA team
spinach
-
-
Manually annotated by BRENDA team
strain RN4220, gene coaA
-
-
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
metabolism
pantothenate kinase catalyzes the rate-controlling step in coenzyme A biosynthesis; pantothenate kinase catalyzes the rate-controlling step in coenzyme A biosynthesis
physiological function
additional information
-
mitochondria-targeted human pantothenate kinase-2 is involved in pantothenate kinase-associated neurodegeneration
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + (R)-4-phosphopantoate + beta-alanine
AMP + diphosphate + (R)-4'-phosphopantothenate
show the reaction diagram
the enzyme is involved in coenzyme A biosynthesis in the archaea
-
-
?
ATP + (R)-pantothenate
ADP + (R)-4'-phosphopantothenate
show the reaction diagram
ATP + D-pantothenate
ADP + 4'-phosphopantothenate
show the reaction diagram
ATP + D-pantothenate
ADP + D-4'-phosphopantothenate
show the reaction diagram
ATP + N-alkylpantothenamides
ADP + ?
show the reaction diagram
growth-inhibiting anti-metabolite, modeling into the active site structure
-
-
?
ATP + N-heptylpantothenamide
ADP + N-heptylpantothenamide 4-phosphate
show the reaction diagram
ATP + N-nonylpantothenamide
ADP + N-nonylpantothenamide 4-phosphate
show the reaction diagram
ATP + N-pentylpantothenamide
ADP + N-pentylpantothenamide 4-phosphate
show the reaction diagram
ATP + pantothenate
?
show the reaction diagram
-
-
-
-
?
ATP + pantothenate
ADP + (R)-4'-phosphopantothenate
show the reaction diagram
ATP + pantothenate
ADP + (R)-4-phosphopantothenate
show the reaction diagram
-
-
-
-
?
ATP + pantothenate
ADP + 4'-phosphopantothenate
show the reaction diagram
ATP + pantothenate
ADP + D-4'-phosphopantothenate
show the reaction diagram
-
-
-
-
?
ATP + pantothenol
ADP + 4'-phosphopantothenol
show the reaction diagram
CTP + (R)-pantothenate
CDP + (R)-4'-phosphopantothenate
show the reaction diagram
-
-
-
?
GTP + (R)-pantothenate
GDP + (R)-4'-phosphopantothenate
show the reaction diagram
GTP + pantothenate
GDP + D-4'-phosphopantothenate
show the reaction diagram
ITP + pantothenate
IDP + D-4'-phosphopantothenate
show the reaction diagram
-
phosphorylation at 46% the rate of ATP
-
-
?
pantetheine + ATP
D-4'-phosphopantetheine + ADP
show the reaction diagram
-
-
-
-
?
panthenoylcysteine + ATP
D-4'-phosphopanthenoylcysteine + ADP
show the reaction diagram
-
-
-
-
?
pantothenate + ATP
4'-phosphopantothenate + ADP
show the reaction diagram
pantothenate + ATP
phosphopantothenate + ADP
show the reaction diagram
pantothenate + CTP
4'-phosphopantothenate + CDP
show the reaction diagram
-
-
-
-
?
pantothenate + dATP
4'-phosphopantothenate + dADP
show the reaction diagram
-
-
-
-
?
pantothenate + dCTP
4'-phosphopantothenate + dCDP
show the reaction diagram
-
-
-
-
?
pantothenate + dGTP
4'-phosphopantothenate + dGDP
show the reaction diagram
-
-
-
-
?
pantothenate + dTTP
4'-phosphopantothenate + dTDP
show the reaction diagram
-
-
-
-
?
pantothenate + UTP
4'-phosphopantothenate + UDP
show the reaction diagram
-
-
-
-
?
pantothenyl alcohol + ATP
D-4'-phosphopantothenyl alcohol + ADP
show the reaction diagram
-
-
-
-
?
UTP + pantothenate
UDP + D-4'-phosphopantothenate
show the reaction diagram
-
phosphorylation at 18% the rate of ATP
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + (R)-4-phosphopantoate + beta-alanine
AMP + diphosphate + (R)-4'-phosphopantothenate
show the reaction diagram
Q5JHF1
the enzyme is involved in coenzyme A biosynthesis in the archaea
-
-
?
ATP + (R)-pantothenate
ADP + (R)-4'-phosphopantothenate
show the reaction diagram
ATP + D-pantothenate
ADP + D-4'-phosphopantothenate
show the reaction diagram
ATP + N-heptylpantothenamide
ADP + N-heptylpantothenamide 4-phosphate
show the reaction diagram
ATP + pantothenate
ADP + (R)-4'-phosphopantothenate
show the reaction diagram
-
first step of the pathway from pantothenate to CoA. The activity of this protein is tightly regulated by CoA feedback inhibition, both in vitro and in vivo
-
-
?
ATP + pantothenate
ADP + 4'-phosphopantothenate
show the reaction diagram
Q8TE04, Q9H999
pantothenate kinase catalyzes the first step in CoA biosynthesis
-
-
?
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
CTP
less active than ATP
GTP
less active than ATP
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
-
activation, can replace Mg2+
Ni2+
-
activation, can replace Mg2+ with about 50% efficiency
Zn2+
-
activation, can replace Mg2+ with about 50% efficiency
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(2R)-2,4-dihydroxy-3,3-dimethyl-N'-phenylbutanohydrazide
(2R)-2,4-dihydroxy-3,3-dimethylbutanohydrazide
-
competitive, pantothenic acid analogue with 2,4-dihydroxy-3,3-dimethylbutyramide core of pantothenate, inhibition mechanism
(2R)-2,4-dihydroxy-N-(2-hydroxyethyl)-3,3-dimethylbutanamide
-
competitive, pantothenic acid analogue with 2,4-dihydroxy-3,3-dimethylbutyramide core of pantothenate, inhibition mechanism
(2R)-N-(2,3-dihydroxypropyl)-2,4-dihydroxy-3,3-dimethylbutanamide
-
competitive, pantothenic acid analogue with 2,4-dihydroxy-3,3-dimethylbutyramide core of pantothenate, inhibition mechanism
(2R)-N-allyl-2,4-dihydroxy-3,3-dimethylbutanamide
(R)-3-azido-4,4-dimethyl-dihydro-furan-2-one
(R)-4-(2,4-dihydroxy-3,3-dimethyl-butyrylamino)-butyric acid
(R)-4-(2-amino-4-hydroxy-3,3-dimethyl-butyrylamino)-butyric acid
(R)-4-(2-azido-4-hydroxy-3,3-dimethyl-butyrylamino)-butyric acid
(S)-4-(2,4-dihydroxy-3,3-dimethyl-butyrylamino)-butyric acid
(S)-trifluoro-methanesulfonic acid 4,4-dimethyl-2-oxo-tetrahydro-furan-3-yl-ester
1,4-dioxa-8-azaspiro[4.5]dec-8-yl[2-[(3-fluorophenyl)sulfanyl]pyridin-4-yl]methanone
-
mixed non-competitive inhibition
1,4-phenylene-bis(1,2-ethanediyl)bis-isothiourea dihydrobromide
-
2'-Ketopantetheine
-
-
2,4-dihydroxy-3,3-dimethyl N-(2-heptylcarbamoyl-ethyl)-butyramide
2,4-dihydroxy-3,3-dimethyl N-(2-isobutylcarbamoyl-ethyl)-butyramide
2,4-dihydroxy-3,3-dimethyl N-(2-pentylcarbamoyl-ethyl)-butyramide
2,4-dihydroxy-3,3-dimethyl N-(2-phenethylcarbamoyl-ethyl)-butyramide
2,4-dihydroxy-3,3-dimethyl N-(2-propylcarbamoylethyl)-butyramide
2,4-dihydroxy-3,3-dimethyl N-(2-[1-methyl-3-phenyl-propylcarbamoyl]-ethyl)-butyramide
2,4-dihydroxy-3,3-dimethyl N-(2-[2-(3,4-dimethoxy-phenyl)-ethylcarbamoyl]-ethyl)-butyramide
2,4-dihydroxy-3,3-dimethyl N-(2-[3,4,5-trimethoxy-benzylcarbamoyl]-ethyl)-butyramide
2,4-dihydroxy-3,3-dimethyl N-[2-(2,6,6-trimethylbicyclo[3.1.1]hept-3-ylcarbamoyl)-ethyl]-butyramide
2,4-dihydroxy-3,3-dimethyl N-[2-(2-ethoxy-ethylcarbamoyl)-ethyl]-butyramide
2,4-dihydroxy-3,3-dimethyl N-[2-(2-ethylsulfanylethylcarbamoyl)-ethyl]-butyramide
2,4-dihydroxy-3,3-dimethyl N-[2-(2-methylsulfanylethylcarbamoyl)-ethyl]-butyramide
2,4-dihydroxy-3,3-dimethyl N-[2-(2-morpholin-4-yl-ethylcarbamoyl)-ethyl]-butyramide
2,4-dihydroxy-3,3-dimethyl N-[2-(3,7-dimethylocta-2,6-dienylcarbamoyl)-ethyl]-butyramide
2,4-dihydroxy-3,3-dimethyl N-[2-(3-ethoxy-propylcarbamoyl)-ethyl]-butyramide
2,4-dihydroxy-3,3-dimethyl N-[2-(3-methylsulfanylpropylcarbamoyl)-ethyl]-butyramide
2,4-dihydroxy-3,3-dimethyl N-[2-(4-methoxy-benzylcarbamoyl)-ethyl]-3,3-dimethyl-butyramide
2,4-dihydroxy-3,3-dimethyl N-[3-(4-benzyl-piperazin-1-yl)-3-oxo-propyl]-butyramide
2,4-dihydroxy-N-[2-(2-hydroxy-ethylcarbamoyl)-ethyl]-3,3-dimethyl-butyramide
2,4-dihydroxy-N-[2-(2-methoxy-ethylcarbamoyl)-ethyl]-3,3-dimethyl-butyramide
2,4-dihydroxy-N-[2-(3-hydroxy-propylcarbamoyl)-ethyl]-3,3-dimethyl-butyramide
2,4-dihydroxy-N-[2-(3-methoxy-propylcarbamoyl)-ethyl]-3,3-dimethyl-butyramide
2-[2-(1-benzoylpiperidin-4-yl)-5-methyl-1,3-thiazol-4-yl]-N-(4-methylphenyl)acetamide
-
uncompetitive inhibition
4-(2,4-dihydroxy-3,3-dimethylbutylamido)butyric acid
-
competitive inhibitor; competitive inhibitor, IC50: 0.05-0.15 mM
4-phosphopantoate
substrate inhibition
5'-deoxy-5'-(4-(beta-D-galactopyranosyloxymethyl)-1,2,3-triazol-1-yl)adenosine
-
competitive inhibitor with respect to ATP
acetoacetyl-CoA
-
-
acetyl-CoA
Acyl carrier protein
acyl-CoA-esters
Arachidonoyl-CoA
-
-
chloranil
-
CoA esters
-
feedback inhibition, inhibition kinetics of recombinant isozyme mPanK2
coenzyme A
D-pantothenate
-
substrate inhibition, above 0.5 mM
dehydroisoandrosterone
about 30% residual activity at 0.1 mM (isoform PanK3)
Dehydroisoandrosterone sulfate
; about 8% residual activity at 0.1 mM (isoform PanK3)
dephospho-CoA
diphosphate
-
-
ephedrine hydrochloride
-
estradiol
about 80% residual activity at 0.1 mM (isoform PanK3)
Estradiol sulfate
about 90% residual activity at 0.1 mM (isoform PanK3)
Estrone sulfate
about 50% residual activity at 0.1 mM (isoform PanK3)
Fusidic acid
-
glipizide
-
glyburide
-
Hexachlorophene
-
L-Pantothenate
-
-
malonyl-CoA
MCC-555
MCC-555 inhibits all three isoforms with a rank order of PanK3 > PanK2 > PanK1b; MCC-555 inhibits all three isoforms with a rank order of PanK3 > PanK2 > PanK1b
N-heptylpantothenamide
N-pentylpantothenamide
N-[1-(5-[[2-(4-chlorophenoxy)ethyl]sulfanyl]-4-methyl-4H-1,2,4-triazol-3-yl)ethyl]naphthalene-1-carboxamide
-
competitive inhibition
Octanoyl-CoA
-
-
oleoyl-CoA
-
-
palmitoyl-CoA
pantetheine 4'-phosphate
pantothenamide, N-substituted
-
IC50 about 0.0004-0.0016 mM
-
pantothenic acid 4'-phosphate
-
-
pantothenol
-
competitive inhibition
pantothenoylcysteine 4'-phosphate
pantothenyl alcohol
-
-
pioglitazone hydrochloride
-
pregnenolone sulfate
; about 10% residual activity at 0.1 mM (isoform PanK3); about 92% residual activity at 0.1 mM (isoform PanK3)
propionyl-CoA
-
-
Psi-rhodomyrtoxin
-
Reactive blue 2
-
Ro 41-0960
-
rosiglitazone
-
succinyl-CoA
-
-
tamoxifen
-
tolfenamic acid
-
tyrphostin AG 528
-
tyrphostin AG 808
-
WIN 62577
-
[2-[(3-chlorophenyl)sulfanyl]pyridin-4-yl][4-(hydroxymethyl)piperidin-1-yl]methanone
-
mixed non-competitive inhibition
[2-[(3-chlorophenyl)sulfanyl]quinolin-4-yl](piperidin-1-yl)methanone
-
mixed non-competitive inhibition
[[2-[[4-(6-methylpyridin-2-yl)piperazin-1-yl]methyl]-4'-(trifluoromethoxy)biphenyl-4-yl]oxy]acetic acid
-
mixed non-competitive inhibition
additional information
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Acyl carrier protein
-
PAK II: inhibition, PAK I: stimulation between 0.015-0.035 mM
cholesterol
about 125% activity at 0.1 mM
CoA
-
stimulates
dithiothreitol
-
activation
glyburide
; isoform PanK1b is activated by glyburide
O-oleoylcarnitine
140% activation at 0.1 mM
O-palmitoylcarnitine
120% activation at 0.1 mM
oleoylethanolamide
120% activation at 0.1 mM
palmitoylcarnitine
tamoxifen
140% activation at 0.1 mM
additional information
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0057 - 0.168
(R)-pantothenate
0.027 - 9.59
ATP
1.759
CTP
-
-
0.138
dATP
-
-
0.839
dCTP
-
-
0.117
dGTP
-
-
0.723
dTTP
-
-
668
GTP
-
in citrate buffer, pH 6.5, temperature not specified in the publication
0.225 - 1
MgATP2-
0.008 - 0.124
N-heptylpantothenamide
0.003 - 0.14
N-pentylpantothenamide
0.009 - 1.3
pantothenate
0.25 - 0.28
pantothenol
1.715
UTP
-
-
additional information
additional information
-
kinetics of recombinant isozyme mPanK2
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.29 - 2.12
(R)-pantothenate
0.052 - 2.12
ATP
1.5
CTP
Bacillus anthracis
-
-
0.9
dATP
Bacillus anthracis
-
-
0.5
dCTP
Bacillus anthracis
-
-
0.8
dGTP
Bacillus anthracis
-
-
0.4
dTTP
Bacillus anthracis
-
-
0.064
GTP
Mycobacterium tuberculosis
-
in citrate buffer, pH 6.5, temperature not specified in the publication
0.425
N-heptylpantothenamide
Staphylococcus aureus
-
pH 7.5, 37C, recombinant enzyme
0.15
N-pentylpantothenamide
Staphylococcus aureus
-
pH 7.5, 37C, recombinant enzyme
0.61 - 0.818
pantothenate
0.38 - 0.4
pantothenol
1.7
UTP
Bacillus anthracis
-
-
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.08 - 9.91
ATP
0.9
CTP
Bacillus anthracis
-
-
60
6.6
dATP
Bacillus anthracis
-
-
140
0.6
dCTP
Bacillus anthracis
-
-
179
6.5
dGTP
Bacillus anthracis
-
-
219
0.5
dTTP
Bacillus anthracis
-
-
145
0.095
GTP
Mycobacterium tuberculosis
-
in citrate buffer, pH 6.5, temperature not specified in the publication
37
1
UTP
Bacillus anthracis
-
-
65
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.138 - 0.2
(2R)-2,4-dihydroxy-3,3-dimethyl-N'-phenylbutanohydrazide
0.013
(2R)-2,4-dihydroxy-3,3-dimethylbutanohydrazide
-
37C, versus pantothenate at 1 mM
0.0019
(2R)-2,4-dihydroxy-N-(2-hydroxyethyl)-3,3-dimethylbutanamide
-
37C, versus pantothenate at 1 mM
4
(2R)-N-(2,3-dihydroxypropyl)-2,4-dihydroxy-3,3-dimethylbutanamide
-
37C, versus pantothenate at 1 mM
0.082 - 1
(2R)-N-allyl-2,4-dihydroxy-3,3-dimethylbutanamide
0.162
2,4-dihydroxy-3,3-dimethyl N-(2-pentylcarbamoyl-ethyl)-butyramide
-
37C, versus pantothenate at 1 mM
0.112
2,4-dihydroxy-3,3-dimethyl N-(2-propylcarbamoylethyl)-butyramide
-
37C, versus pantothenate at 1 mM
0.111
2,4-dihydroxy-3,3-dimethyl N-[2-(3-ethoxy-propylcarbamoyl)-ethyl]-butyramide
-
37C, versus pantothenate at 1 mM
0.076
2,4-dihydroxy-3,3-dimethyl N-[2-(3-methylsulfanylpropylcarbamoyl)-ethyl]-butyramide
-
37C, versus pantothenate at 1 mM
0.109
2,4-dihydroxy-N-[2-(3-methoxy-propylcarbamoyl)-ethyl]-3,3-dimethyl-butyramide
-
37C, versus pantothenate at 1 mM
0.164
5'-deoxy-5'-(4-(beta-D-galactopyranosyloxymethyl)-1,2,3-triazol-1-yl)adenosine
-
-
0.002 - 0.067
CoA
0.037
pantetheine 4'-phosphate
-
pH 6.5, 37C
0.125
pantothenic acid 4'-phosphate
-
pH 6.5, 37C
0.129
pantothenol
-
37C, versus pantothenate at 1 mM
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0084
1,4-dioxa-8-azaspiro[4.5]dec-8-yl[2-[(3-fluorophenyl)sulfanyl]pyridin-4-yl]methanone
Mycobacterium tuberculosis
-
in 50 mM PIPES-NaOH (pH 7.0), 25 mM KCl, 20 mM MgCl, at 25C
0.0004
1,4-phenylene-bis(1,2-ethanediyl)bis-isothiourea dihydrobromide
Homo sapiens
Q9H999
isoform PanK3, at pH 7.5 and 37C
0.00257
2-[2-(1-benzoylpiperidin-4-yl)-5-methyl-1,3-thiazol-4-yl]-N-(4-methylphenyl)acetamide
Mycobacterium tuberculosis
-
in 50 mM PIPES-NaOH (pH 7.0), 25 mM KCl, 20 mM MgCl, at 25C
0.05 - 0.15
4-(2,4-dihydroxy-3,3-dimethylbutylamido)butyric acid
Mus musculus
-
competitive inhibitor, IC50: 0.05-0.15 mM
0.00006 - 0.000125
acetyl-CoA
0.0059
chloranil
Homo sapiens
Q9H999
isoform PanK3, at pH 7.5 and 37C
0.2
CoA
Plasmodium falciparum
-
IC50: 0.2 mM
0.0035
Dehydroisoandrosterone sulfate
Homo sapiens
Q9H999
isoform PanK3, at pH 7.5 and 37C
0.0016
ephedrine hydrochloride
Homo sapiens
Q9H999
isoform PanK3, at pH 7.5 and 37C
0.0001
Fusidic acid
Homo sapiens
Q9H999
isoform PanK3, at pH 7.5 and 37C
0.0052
glipizide
Homo sapiens
Q9H999
isoform PanK3, at pH 7.5 and 37C
0.0068
glyburide
Homo sapiens
Q9H999
isoform PanK3, at pH 7.5 and 37C
0.0092
GW5074
Homo sapiens
Q9H999
isoform PanK3, at pH 7.5 and 37C
0.0088
Hexachlorophene
Homo sapiens
Q9H999
isoform PanK3, at pH 7.5 and 37C
0.0048
N-heptylpantothenamide
Staphylococcus aureus
-
IC50 is 0.0048 mM, potent growth inhibitory anti-metabolite
0.0035
N-pentylpantothenamide
Staphylococcus aureus
-
IC50 is 0.0035 mM, has antimicrobial activity against Staphylococcus aureus
0.00007
N-[1-(5-[[2-(4-chlorophenoxy)ethyl]sulfanyl]-4-methyl-4H-1,2,4-triazol-3-yl)ethyl]naphthalene-1-carboxamide
Mycobacterium tuberculosis
-
in 50 mM PIPES-NaOH (pH 7.0), 25 mM KCl, 20 mM MgCl, at 25C
0.0004 - 0.0016
pantothenamide, N-substituted
Staphylococcus aureus
-
IC50 about 0.0004-0.0016 mM
-
0.001
pioglitazone hydrochloride
Homo sapiens
Q9H999
isoform PanK3, at pH 7.5 and 37C
0.0025
pregnenolone sulfate
Homo sapiens
Q9H999
isoform PanK3, at pH 7.5 and 37C
0.0016
Psi-rhodomyrtoxin
Homo sapiens
Q9H999
isoform PanK3, at pH 7.5 and 37C
0.0013
Reactive blue 2
Homo sapiens
Q9H999
isoform PanK3, at pH 7.5 and 37C
0.0039
Ro 41-0960
Homo sapiens
Q9H999
isoform PanK3, at pH 7.5 and 37C
0.0017
rosiglitazone
Homo sapiens
Q9H999
isoform PanK3, at pH 7.5 and 37C
0.0092
tolfenamic acid
Homo sapiens
Q9H999
isoform PanK3, at pH 7.5 and 37C
0.003
tyrphostin AG 528
Homo sapiens
Q9H999
isoform PanK3, at pH 7.5 and 37C
0.009
tyrphostin AG 808
Homo sapiens
Q9H999
isoform PanK3, at pH 7.5 and 37C
0.0007
WIN 62577
Homo sapiens
Q9H999
isoform PanK3, at pH 7.5 and 37C
0.00024 - 0.00042
[2-[(3-chlorophenyl)sulfanyl]pyridin-4-yl][4-(hydroxymethyl)piperidin-1-yl]methanone
0.00087
[2-[(3-chlorophenyl)sulfanyl]quinolin-4-yl](piperidin-1-yl)methanone
Mycobacterium tuberculosis
-
in 50 mM PIPES-NaOH (pH 7.0), 25 mM KCl, 20 mM MgCl, at 25C
0.00082
[[2-[[4-(6-methylpyridin-2-yl)piperazin-1-yl]methyl]-4'-(trifluoromethoxy)biphenyl-4-yl]oxy]acetic acid
Mycobacterium tuberculosis
-
in 50 mM PIPES-NaOH (pH 7.0), 25 mM KCl, 20 mM MgCl, at 25C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0002421
-
0.045 mM pantothenate, 0.25 mM ATP (pH 7.0), 10 mM MgCl2, 0.1 M Tris/HCl (pH 7.5), at 37C
0.0012
-
pH 6.1, 37C
0.00171
-
pH 7.0, 37C
0.0041
-
pH 6.1, 37C
0.034
-
pH 6.5, 37C
0.152
-
isoform 1, 37C, pH 8.0
0.36
purified recombinant protein
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.9 - 6.3
-
-
6 - 9
-
broad, highest activity in 50 mM phosphate buffer
7
-
assay at
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.5 - 6
more than 70% of the maximum activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
15
the enzyme has no activity at temperatures below 15C
30 - 42
-
-
50 - 70
more than 80% of the maximum activity
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.1
-
isoelectric focusing, isoform B
5.7
-
isoelectric focusing, isoform A
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
additional information
-
multiple subcellular distribution of multiple enzyme forms
-
Manually annotated by BRENDA team
PDB
SCOP
CATH
ORGANISM
UNIPROT
Burkholderia cenocepacia (strain ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 / J2315 / CF5610)
Burkholderia thailandensis (strain E264 / ATCC 700388 / DSM 13276 / CIP 106301)
Burkholderia thailandensis (strain E264 / ATCC 700388 / DSM 13276 / CIP 106301)
Coxiella burnetii (strain RSA 493 / Nine Mile phase I)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Legionella pneumophila subsp. pneumophila (strain Philadelphia 1 / ATCC 33152 / DSM 7513)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Staphylococcus aureus (strain MSSA476)
Staphylococcus aureus (strain MSSA476)
Staphylococcus aureus (strain MW2)
Staphylococcus aureus (strain MW2)
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
36000
-
SDS-PAGE
36300
-
calculated from sequence of cDNA
45000
-
gel filtration
48000
-
SDS-PAGE, mature protein
59000
-
recombinant enzyme, gel filtration
99600
-
calculated from amino acid sequence
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexamer
-
X-ray crystallography, in the crystal form
homodimer
-
2 * 36000, SDS-PAGE
monomer
x-ray crystallography
additional information
formation of a pantothenate kinase-ADP-pantothenate ternary complex, structure determination, pantothenate binding to the enzyme induces a significant conformational change in amino acids 243263, which form a lid that folds over the open pantothenate binding groove
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
proteolytic modification
-
several isoforms of PanK2 are produced by sequential proteolytic cleavage, identification of cleavage sites for the mitochondrial processing peptidase, overview
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
sitting-drop method. The crystal structure of Bacillus anthracis PanK is solved using multiwavelength anomalous dispersion data and refined at a resolution of 2.0 A; sitting drop vapour diffusion method using 24-26% ethylene glycol
purified recombinant enzyme in a ternary complex with ADP and pantothenate, hanging drop vapour diffusion method, 30 mg/ml protein in 20 mM Tris-HCl, pH 8.0, 1 mM DTT, 1 mM EDTA, incubated overnight with 30 mM ADP, 30 mM pantothenate, and 30 mM magnesium nitrate, mixing in equal volumes with reservoir solution containing 11% PEG 3350, 0.2 M sodium citrate, pH 8.2, at 18C, 1 week, X-ray diffraction structure determination and analysis at 2.2 A resolution, molecular modeling of N-alkylpantothenamides, growth-inhibitory anti-metabolites
sitting drop vapor diffusion method at 18C, homodimeric structures of the catalytic core of PanK1alpha in complex with acetyl-CoA. Crystallographic mapping of missense mutations associated with pantothenate kinase-associated neurodegeneration disease; sitting drop vapor diffusion method at 18C, homodimeric structures of the catalytic core of PanK3 in complex with acetyl-CoA. Crystallographic mapping of missense mutations associated with pantothenate kinase-associated neurodegeneration disease
bound to oantothenate, pantothenol or N-nonylpantothenamide, using 1.4-1.8 M trisodium citrate, 0.05-0.1 M sodium acetate and 10% (v/v) glycerol at pH 6.5
-
complexes of the enzyme with guanosine-5'-[(beta,gamma)-methyleno]triphosphate and pantothenate, GDP and phosphopantothenate, GDP, GDP and pantothenate, 5'-adenylyl (beta,gamma-methylene)diphosphonate, and guanosine-5'-[(beta,gamma)-methyleno]triphosphate, hanging drop vapor diffusion method, using 1.4-1.8 M trisodium citrate, 0.05-0.1 M sodium acetate, and 10% (v/v) glycerol at pH 6.5
-
hanging drop vapour diffusion method using 10-15% (w/v) PEG 8000, 0.05-0.1 M NaOAc and 0.05 M NaCl dissolved in 0.1 M sodium cacodylate buffer pH 6.5
purified recombinant His-tagged enzyme, hanging drop vapour diffusion method, 0.003 ml protein solution containing 6 mg/ml protein, 0.1 M Tris-HCl, pH 8.0, 0.15 M NaCl, 5% v/v glycerol and 0.001 M 2-mercaptoethanol, mixed 0.001 ml of precipitant solution containing 10-15% w/v PEG 8000, 0.05-0.1 M NaOAc, 0.05 NaCl, and 0.1 M cacodylate, pH 6.5, room temperature, equilibration against 0.4 ml precipitant solution, 3-7 days, 2 different crystal forms, X-ray diffraction structure determination and analysis at 2.5 A and 2.9 A resolution, respectively, molecular replacement calculations
-
hanging drop vapour diffusion method using 28% (w/v) PEG 2000 mM and 0.1M Bis-Tris (pH 6.5), at 18C
sitting drop vapour diffusion method using 28% (w/v) PEG 2 M and 0.1 M Bis-Tris (pH 6.5), at 18C
hanging drop vapor-diffusion method, crystal structures of PanK-III in complex with pantothenate, phosphopantothenate as well as a ternary complex structure of PanK-III with pantothenate and ADP
sitting drop vapour diffusion method with 15% polyethylene glycol 3350
-
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5
-
t1/2: 10 min at 30C
641389
7
-
10 min stable at 30C
641389
7.5
-
90% of activity retained after 10 min at 30C
641389
8
-
80% of activity retained after 10 min at 30C
641389
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
-
10% to 20% loss of activity within 10 min at pH 7.5 to 8, 10 min stable at pH 7, t1/2: 10 min at pH 6.5
37
-
10 min, 70% loss of activity
43
-
10 min, inactivation
47
-
loses kinase activity at temperatures higher than 47C
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE