General Stability | Organism |
---|---|
highly unstable upon purification, e.g. successive chromatography | Rattus norvegicus |
thiol reducing agents stabilize enzyme in solution | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | - |
Rattus norvegicus | |
acyl-CoA-esters | - |
Rattus norvegicus | |
ATP | free form | Rattus norvegicus | |
citrate | - |
Rattus norvegicus | |
CoA | not reversible by D-carnitine, acetyl-L-carnitine or other carnitine analogs; reversible by L-carnitine | Rattus norvegicus | |
dephospho-CoA | - |
Rattus norvegicus | |
diphosphate | - |
Rattus norvegicus | |
additional information | not inhibitory: Mg2+, NADP+; not inhibitory: NAD+ | Rattus norvegicus | |
palmitoyl-CoA | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.018 | - |
pantothenate | pH 7.0, 37°C | Rattus norvegicus | |
0.6 | - |
ATP | pH 7.0, 37°C | Rattus norvegicus | |
0.6 | - |
ATP | ATP in form of MgATP2- | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | Km-value: 0.6 mM | Rattus norvegicus | |
Mg2+ | requirement, active substrate: MgATP-complex | Rattus norvegicus | |
additional information | nonspecific activation by anions in triethanolamine buffer, pH 7, mostly dicarboxylic acids, not citrate or diphosphate | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-pantothenate | Rattus norvegicus | - |
ADP + D-4'-phosphopantothenate | - |
? | |
ATP + D-pantothenate | Rattus norvegicus Sprague-Dawley | - |
ADP + D-4'-phosphopantothenate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
Sprague-Dawley | - |
Rattus norvegicus Sprague-Dawley | - |
Sprague-Dawley | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
heart | ventricular muscle | Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00171 | - |
pH 7.0, 37°C | Rattus norvegicus |
Storage Stability | Organism |
---|---|
-65°C, 3 mg partially purified protein/ml, at least 4 months | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (R)-pantothenate | - |
Rattus norvegicus | ADP + (R)-4'-phosphopantothenate | - |
? | |
ATP + (R)-pantothenate | - |
Rattus norvegicus Sprague-Dawley | ADP + (R)-4'-phosphopantothenate | - |
? | |
ATP + D-pantothenate | - |
Rattus norvegicus | ADP + D-4'-phosphopantothenate | - |
? | |
ATP + D-pantothenate | - |
Rattus norvegicus Sprague-Dawley | ADP + D-4'-phosphopantothenate | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | broad, highest activity in 50 mM phosphate buffer | Rattus norvegicus |