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Literature summary for 2.7.1.33 extracted from

  • Das, S.; Kumar, P.; Bhor, V.; Surolia, A.; Vijayan, M.
    Invariance and variability in bacterial PanK: a study based on the crystal structure of Mycobacterium tuberculosis PanK (2006), Acta Crystallogr. Sect. D, 62, 628-638.
    View publication on PubMed
Search for more literature for 2.7.1.33

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) cells Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapour diffusion method using 10-15% (w/v) PEG 8000, 0.05-0.1 M NaOAc and 0.05 M NaCl dissolved in 0.1 M sodium cacodylate buffer pH 6.5 Mycobacterium tuberculosis

Inhibitors

Inhibitors Comment Organism Structure
CoA feedback inhibitor Escherichia coli
CoA feedback inhibitor Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
 2 * 36000, SDS-PAGE Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P0A6I3
-
-
Mycobacterium tuberculosis P9WPA7
-
-
Mycobacterium tuberculosis H37Rv P9WPA7
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA metal affinity column chromatography Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (R)-pantothenate
-
 Mycobacterium tuberculosis ADP + (R)-4'-phosphopantothenate
-
 ?
ATP + (R)-pantothenate
-
 Escherichia coli ADP + (R)-4'-phosphopantothenate
-
 ?
ATP + (R)-pantothenate
-
 Mycobacterium tuberculosis H37Rv ADP + (R)-4'-phosphopantothenate
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 36000, SDS-PAGE Escherichia coli

Synonyms

Synonyms Comment Organism
PanK
-
 Mycobacterium tuberculosis
PanK
-
 Escherichia coli

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Mycobacterium tuberculosis
ATP
-
 Escherichia coli