Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | enzyme is not affected by CoA or acetyl-CoA | Bacillus subtilis | |
additional information | enzyme is not affected by CoA or acetyl-CoA | Helicobacter pylori |
Cloned (Comment) | Organism |
---|---|
gene coaX, gene cluster analysis, DNA and amino acid sequence determination and analysis, overexpression of N-terminally His6-tagged in Escherichia coli strain BL21(DE3) | Bacillus subtilis |
gene coaX, gene cluster analysis, DNA and amino acid sequence determination and analysis, overexpression of N-terminally His6-tagged in Escherichia coli strain BL21(DE3) | Helicobacter pylori |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no inhibition by N-pentylpantothenamide, enzyme is not affected by CoA or acetyl-CoA | Bacillus subtilis | |
additional information | no inhibition by N-pentylpantothenamide, enzyme is not affected by CoA or acetyl-CoA | Helicobacter pylori |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.101 | - |
(R)-pantothenate | pH 7.6, 25°C | Helicobacter pylori | |
0.168 | - |
(R)-pantothenate | pH 7.6, 25°C | Bacillus subtilis | |
3.05 | - |
ATP | pH 7.6, 25°C | Bacillus subtilis | |
9.59 | - |
ATP | pH 7.6, 25°C | Helicobacter pylori |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | - |
Bacillus subtilis | |
KCl | - |
Helicobacter pylori | |
Mg2+ | - |
Bacillus subtilis | |
Mg2+ | - |
Helicobacter pylori |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (R)-pantothenate | Bacillus subtilis | first step in coenzyme A biosynthesis | ADP + (R)-4'-phosphopantothenate | - |
? | |
ATP + (R)-pantothenate | Helicobacter pylori | first step in coenzyme A biosynthesis | ADP + (R)-4'-phosphopantothenate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
gene coaX | - |
Helicobacter pylori | p37564 | gene coaX | - |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Bacillus subtilis |
recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Helicobacter pylori |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (R)-pantothenate | - |
Helicobacter pylori | ADP + (R)-4'-phosphopantothenate | - |
? | |
ATP + (R)-pantothenate | first step in coenzyme A biosynthesis | Bacillus subtilis | ADP + (R)-4'-phosphopantothenate | - |
? | |
ATP + (R)-pantothenate | first step in coenzyme A biosynthesis | Helicobacter pylori | ADP + (R)-4'-phosphopantothenate | - |
? | |
ATP + (R)-pantothenate | the enzyme is absolutely specific for ATP, no activity with CTP, GTP, UTP, or phosphoenolpyruvate as phosphoryl donors | Bacillus subtilis | ADP + (R)-4'-phosphopantothenate | - |
? | |
CTP + (R)-pantothenate | - |
Helicobacter pylori | CDP + (R)-4'-phosphopantothenate | - |
? | |
GTP + (R)-pantothenate | - |
Helicobacter pylori | GDP + (R)-4'-phosphopantothenate | - |
? | |
additional information | N-pentylpantothenate is no substrate | Bacillus subtilis | ? | - |
? | |
additional information | N-pentylpantothenate is no substrate, no activity with UTP or phosphoenolpyruvate as phosphoryl donors | Helicobacter pylori | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CoaX | - |
Bacillus subtilis |
CoaX | - |
Helicobacter pylori |
More | the enzyme forms a type III pantothenate kinase | Bacillus subtilis |
More | the enzyme forms a type III pantothenate kinase | Helicobacter pylori |
PanK | - |
Bacillus subtilis |
PanK | - |
Helicobacter pylori |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Bacillus subtilis |
25 | - |
assay at | Helicobacter pylori |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.09 | - |
ATP | pH 7.6, 25°C | Helicobacter pylori | |
2.09 | - |
(R)-pantothenate | pH 7.6, 25°C | Helicobacter pylori | |
2.12 | - |
ATP | pH 7.6, 25°C | Bacillus subtilis | |
2.12 | - |
(R)-pantothenate | pH 7.6, 25°C | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Bacillus subtilis |
7.6 | - |
assay at | Helicobacter pylori |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | absolutely specific for | Bacillus subtilis | |
ATP | can partially be substituted by CTP or GTP | Helicobacter pylori | |
CTP | less active than ATP | Helicobacter pylori | |
GTP | less active than ATP | Helicobacter pylori |