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Literature summary for 2.7.1.33 extracted from
- Characterization of a new pantothenate kinase isoform from Helicobacter pylori (2005), J. Biol. Chem., 280, 20185-20188.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | enzyme is not affected by CoA or acetyl-CoA | Bacillus subtilis | |
| additional information | enzyme is not affected by CoA or acetyl-CoA | Helicobacter pylori |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene coaX, gene cluster analysis, DNA and amino acid sequence determination and analysis, overexpression of N-terminally His6-tagged in Escherichia coli strain BL21(DE3) | Bacillus subtilis |
| gene coaX, gene cluster analysis, DNA and amino acid sequence determination and analysis, overexpression of N-terminally His6-tagged in Escherichia coli strain BL21(DE3) | Helicobacter pylori |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no inhibition by N-pentylpantothenamide, enzyme is not affected by CoA or acetyl-CoA | Bacillus subtilis | |
| additional information | no inhibition by N-pentylpantothenamide, enzyme is not affected by CoA or acetyl-CoA | Helicobacter pylori |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.101 | - |
(R)-pantothenate | pH 7.6, 25°C | Helicobacter pylori |  |
| 0.168 | - |
(R)-pantothenate | pH 7.6, 25°C | Bacillus subtilis | |
| 3.05 | - |
ATP | pH 7.6, 25°C | Bacillus subtilis | |
| 9.59 | - |
ATP | pH 7.6, 25°C | Helicobacter pylori | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| KCl | - |
Bacillus subtilis | |
| KCl | - |
Helicobacter pylori | |
| Mg2+ | - |
Bacillus subtilis | |
| Mg2+ | - |
Helicobacter pylori | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + (R)-pantothenate | Bacillus subtilis | first step in coenzyme A biosynthesis | ADP + (R)-4'-phosphopantothenate | - |
? | |
| ATP + (R)-pantothenate | Helicobacter pylori | first step in coenzyme A biosynthesis | ADP + (R)-4'-phosphopantothenate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
gene coaX | - |
| Helicobacter pylori | p37564 | gene coaX | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Bacillus subtilis |
| recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Helicobacter pylori |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + (R)-pantothenate | - |
Helicobacter pylori | ADP + (R)-4'-phosphopantothenate | - |
? | |
| ATP + (R)-pantothenate | first step in coenzyme A biosynthesis | Bacillus subtilis | ADP + (R)-4'-phosphopantothenate | - |
? | |
| ATP + (R)-pantothenate | first step in coenzyme A biosynthesis | Helicobacter pylori | ADP + (R)-4'-phosphopantothenate | - |
? | |
| ATP + (R)-pantothenate | the enzyme is absolutely specific for ATP, no activity with CTP, GTP, UTP, or phosphoenolpyruvate as phosphoryl donors | Bacillus subtilis | ADP + (R)-4'-phosphopantothenate | - |
? | |
| CTP + (R)-pantothenate | - |
Helicobacter pylori | CDP + (R)-4'-phosphopantothenate | - |
? | |
| GTP + (R)-pantothenate | - |
Helicobacter pylori | GDP + (R)-4'-phosphopantothenate | - |
? | |
| additional information | N-pentylpantothenate is no substrate | Bacillus subtilis | ? | - |
? | |
| additional information | N-pentylpantothenate is no substrate, no activity with UTP or phosphoenolpyruvate as phosphoryl donors | Helicobacter pylori | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CoaX | - |
Bacillus subtilis |
| CoaX | - |
Helicobacter pylori |
| More | the enzyme forms a type III pantothenate kinase | Bacillus subtilis |
| More | the enzyme forms a type III pantothenate kinase | Helicobacter pylori |
| PanK | - |
Bacillus subtilis |
| PanK | - |
Helicobacter pylori |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Bacillus subtilis |
| 25 | - |
assay at | Helicobacter pylori |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.09 | - |
ATP | pH 7.6, 25°C | Helicobacter pylori | |
| 2.09 | - |
(R)-pantothenate | pH 7.6, 25°C | Helicobacter pylori | |
| 2.12 | - |
ATP | pH 7.6, 25°C | Bacillus subtilis | |
| 2.12 | - |
(R)-pantothenate | pH 7.6, 25°C | Bacillus subtilis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
assay at | Bacillus subtilis |
| 7.6 | - |
assay at | Helicobacter pylori |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | absolutely specific for | Bacillus subtilis | |
| ATP | can partially be substituted by CTP or GTP | Helicobacter pylori | |
| CTP | less active than ATP | Helicobacter pylori | |
| GTP | less active than ATP | Helicobacter pylori | |
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