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Information on EC 2.4.1.14 - sucrose-phosphate synthase and Organism(s) Spinacia oleracea and UniProt Accession P31928

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EC Tree
     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.14 sucrose-phosphate synthase
IUBMB Comments
Requires Mg2+ or Mn2+ for maximal activity . The enzyme from Synechocystis sp. strain PCC 6803 is not specific for UDP-glucose as it can use ADP-glucose and, to a lesser extent, GDP-glucose as substrates . The enzyme from rice leaves is activated by glucose 6-phosphate but that from cyanobacterial species is not . While the reaction catalysed by this enzyme is reversible, the enzyme usually works in concert with EC 3.1.3.24, sucrose-phosphate phosphatase, to form sucrose, making the above reaction essentially irreversible . The F in sucrose 6F-phosphate is used to indicate that the fructose residue of sucrose carries the substituent.
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This record set is specific for:
Spinacia oleracea
UNIPROT: P31928
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The taxonomic range for the selected organisms is: Spinacia oleracea
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Synonyms
sps, sucrose phosphate synthase, sucrose-phosphate synthase, sucrose phosphate synthetase, sps a, sucrose-p synthase, spsii, atsps, sps11, sucrose phosphate synthase a, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
sucrose 6-phosphate synthase
-
-
-
-
sucrose phosphate synthetase
-
-
-
-
sucrose phosphate-uridine diphosphate glucosyltransferase
-
-
-
-
sucrosephosphate-UDP glucosyltransferase
-
-
-
-
UDP-glucose-fructose-phosphate glucosyltransferase
-
-
-
-
UDP-glucose:D-fructose-6-phosphate 2-alpha-D-glucosyltransferase
-
-
-
-
uridine diphosphoglucose-fructose phosphate glucosyltransferase
-
-
-
-
additional information
-
two forms of enzyme identified, that differ in regulatory properties and stability
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosyl group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
UDP-glucose:D-fructose-6-phosphate 2-alpha-D-glucosyltransferase
Requires Mg2+ or Mn2+ for maximal activity [2]. The enzyme from Synechocystis sp. strain PCC 6803 is not specific for UDP-glucose as it can use ADP-glucose and, to a lesser extent, GDP-glucose as substrates [2]. The enzyme from rice leaves is activated by glucose 6-phosphate but that from cyanobacterial species is not [2]. While the reaction catalysed by this enzyme is reversible, the enzyme usually works in concert with EC 3.1.3.24, sucrose-phosphate phosphatase, to form sucrose, making the above reaction essentially irreversible [3]. The F in sucrose 6F-phosphate is used to indicate that the fructose residue of sucrose carries the substituent.
CAS REGISTRY NUMBER
COMMENTARY hide
9030-06-2
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
no effect
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5-azido-UDP-glucose
-
nearly complete inhibition at 1 mM
cycloheximide
-
no significant effect on Vmax, reduces extent of light activation
diphosphate
-
slight inhibition
fructose-1,6-bisphosphate
-
-
NaF
-
strong inhibition at 20 mM
okadaic acid
-
-
phosphate
phosphoenolpyruvate
-
slight inhibition
sucrose
-
preincubation increases sensitivity to phosphate inhibition
sucrose-6-phosphate
-
-
Tris-HCl buffer
-
slight inhibition
UDP-glucose
-
-
vanadate
-
-
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,5-Anhydroglucitol 6-phosphate
D-fructose-1-phosphate
-
slight activation
D-glucose-1-phosphate
-
slight activation
fluoride
-
-
glucose-6-phosphate
light
-
mannose
-
pretreatment lowers sensitivity to phosphate inhibition
Mg2+
-
stimulates enzyme activity
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.7 - 9.3
D-fructose 6-phosphate
1.3 - 7.1
UDP-glucose
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.8
fructose-1,6-bisphosphate
-
-
1.75 - 11
phosphate
0.4
sucrose-6-phosphate
-
-
9.4
UDP-glucose
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
57
-
purified enzyme
79.5
-
purified enzyme
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 7.5
-
broad optimum
7.5
-
broad optimum
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
Uniprot
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
low activity
Manually annotated by BRENDA team
additional information
-
no activity in roots
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
SPSA_SPIOL
1056
0
117716
Swiss-Prot
other Location (Reliability: 3)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
117000
-
calculated from DNA sequence
117600
-
calculated from DNA sequence
120000
-
SDS-PAGE
240000
-
native PAGE
253000
-
sucrose density gradient centrifugation
270000 - 280000
-
gel filtration
413000
-
gel filtration of crude leaf extracts
460000
-
gel filtration
480000
-
gel filtration
52000
-
SDS-PAGE, minor band, degradation product
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
-
2 * 120000, sucrose density gradient centrifugation, SDS-PAGE
tetramer
-
4 * 120000
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
-
inactivates highly activated enzyme
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
2-mercaptoethanol and phenol absorbing agents stabilize activity
-
20% ethylene glycol and 0.2 M KCl stabilize activity
-
KF prevents inactivation at room temperature
-
sensitive to freezing and thawing
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-80°C, purified enzyme stable for at least 4 months
-
0°C, 4 weeks, 50% loss of activity
-
4°C, stable for at least 1 week
-
liquid nitrogen, stable for at least 11 months
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
enzyme becomes extremely unstable during the course of purification
-
gel filtration is carried out in presence of 20% ethylene glycol and 0.2 M KCl to stabilize activity
-
in presence of 2-mercaptoethanol and a phenol absorbing agent under N2
-
two forms of the enzyme identified
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
transgenic cotton over-producing spinach sucrose phosphate synthase shows enhanced leaf sucrose synthesis and improved fiber quality under controlled environmental conditions
expression in Escherichia coli and tobacco, active enzyme
-
expression of a 26000 Da fragment in Escherichia coli
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Mendicino, J.
Sucrose phosphate synthesis in wheat germ and green leaves
J. Biol. Chem.
235
3347-3352
1960
Spinacia oleracea, Triticum aestivum
Manually annotated by BRENDA team
Harbron, S.; Foyer, C.; Walker, D.
The purification and properties of sucrose-phosphate synthetase from spinach leaves: the involvement of this enzyme and fructose bisphosphatase in the regulation of sucrose biosynthesis
Arch. Biochem. Biophys.
212
237-246
1981
Spinacia oleracea
Manually annotated by BRENDA team
Amir, J.; Preiss, J.
Kinetic characterization of spinach leaf sucrose phosphate
Plant Physiol.
69
1027-1030
1982
Spinacia oleracea
Manually annotated by BRENDA team
Doehlert, D.C.; Huber, S.C.
Spinach leaf sucrose phosphate snthase. Activation by glucose 6-phosphate and interaction with inorganic phosphate
FEBS Lett.
153
293-297
1983
Spinacia oleracea
-
Manually annotated by BRENDA team
Doehlert, D.C.; Huber, S.C.
Regulation of spinach leaf sucrose phosphate synthase by glucose-6-phosphate, inorganic phosphate and pH
Plant Physiol.
73
989-994
1983
Spinacia oleracea
Manually annotated by BRENDA team
Doehlert, D.C.; Huber, S.C.
Phosphate inhibition of spinach leaf sucrose phosphate synthase is affected by glucose-6-phosphate and phosphoglucoisomerase
Plant Physiol.
76
250-253
1984
Spinacia oleracea
Manually annotated by BRENDA team
Kerr, P.S.; Kalt-Torres, W.; Huber, S.C.
Resolution of two forms of sucrose-phosphate synthase from maize, soybean and spinach leafs
Planta
170
515-519
1987
Glycine max, Spinacia oleracea, Zea mays
Manually annotated by BRENDA team
Walker, J.L.; Huber, S.C.
Purification and preliminary characterization of sucrose-phosphate synthase using monoclonal antibodies
Plant Physiol.
89
518-524
1989
Beta vulgaris, Glycine max, Hordeum vulgare, Spinacia oleracea, Zea mays
Manually annotated by BRENDA team
Huber, S.C.; Huber, J.L.
Activation of sucrose-phosphate synthase from darkened spinach leaves by an endogenous protein phosphatase
Arch. Biochem. Biophys.
282
421-426
1990
Spinacia oleracea
Manually annotated by BRENDA team
Huber, J.L.; Hite, D.R.C.; Outlaw, W.H.; Huber, S.C.
Inactivation of highly activated spinach leaf sucrose-phosphate synthase by dephosphorylation
Plant Physiol.
95
291-297
1991
Spinacia oleracea, Zea mays
Manually annotated by BRENDA team
Huber, S.C.; Huber, J.L.
In vitro phosphorylation and inactivation of spinach leaf sucrose-phosphate synthase by an endogenous protein kinase
Biochim. Biophys. Acta
1091
393-400
1990
Spinacia oleracea
Manually annotated by BRENDA team
Siegl, G.; Stitt, M.
Partial purification of two forms of spinach leaf sucrose-phosphate synthase which differ in their kinetic properties
Plant Sci.
66
205-210
1990
Spinacia oleracea
-
Manually annotated by BRENDA team
Salvucci, M.E.; Drake, R.R.; Haley, B.E.
Purification and photoaffinity labeling of sucrose phosphate synthase from spinach leaves
Arch. Biochem. Biophys.
281
212-218
1990
Spinacia oleracea
Manually annotated by BRENDA team
Klein, R.R.; Crafts-Brander, S.J.; Salvucci, M.E.
Cloning and developmental expression of the sucrose-phosphate-synthase gene from spinach
Planta
190
498-510
1993
Spinacia oleracea
Manually annotated by BRENDA team
Salvucci, M.E.; Klein, R.R.
Identification of the uridine-binding domain of sucrose-phosphate synthase. Expression of a region of the protein that photoaffinity labels with 5-azidouridine diphosphate-glucose
Plant Physiol.
102
529-536
1993
Spinacia oleracea
Manually annotated by BRENDA team
Sinha, A.K.; Pathre, U.; Sane, P.V.
Purification and characterization of sucrose-phosphate synthase from Prosopis juliflora
Phytochemistry
46
441-447
1997
Prosopis juliflora, Spinacia oleracea
-
Manually annotated by BRENDA team
Sonnewald, U.; Quick, W.P.; MacRae, E.; Krause, K.P.; Stitt, M.
Purification, cloning and expression of spinach leaf sucrose-phosphate synthase in Escherichia coli
Planta
189
174-181
1993
Spinacia oleracea
Manually annotated by BRENDA team
Sinha, A.K.; Shirke, P.A.; Pathre, U.; Sane, P.V.
Sucrose-phosphate synthase in tree species: light/dark regulation involves a component of protein turnover in Prosopis juliflora (SW DC)
Biochem. Mol. Biol. Int.
43
421-431
1997
Prosopis juliflora, Spinacia oleracea
Manually annotated by BRENDA team
Pattanayak, D.
Higher plant sucrose-phosphate synthase: structure, function and regulation
Indian J. Exp. Biol.
37
523-529
1999
Beta vulgaris, Glycine max, Oryza sativa, Solanum tuberosum, Spinacia oleracea, Triticum aestivum, Zea mays
-
Manually annotated by BRENDA team
Lutfiyya, L.L.; Xu, N.; DOrdine, R.L.; Morrell, J.A.; Miller, P.W.; Duff, S.M.G.
Phylogenetic and expression analysis of sucrose phosphate synthase isozymes in plants
J. Plant Physiol.
164
923-933
2007
Actinidia chinensis, Actinidia deliciosa, Synechocystis sp., Arabidopsis thaliana, Musa acuminata, Beta vulgaris, Brassica rapa, Vicia faba, Citrus unshiu, Hordeum vulgare, Ipomoea batatas, Medicago sativa, Solanum lycopersicum, Mangifera indica, Nicotiana tabacum, Nostoc punctiforme, Oryza sativa, Pinus pinaster, Prochlorococcus marinus, Saccharum officinarum, Solanum tuberosum, Spinacia oleracea, Triticum aestivum, Viscum album, Zea mays, Gloeobacter violaceus, Thermosynechococcus vestitus, Pirellula sp., Craterostigma plantagineum, Oncidium Goldiana, Synechococcus marinus
Manually annotated by BRENDA team
Haigler, C.H.; Singh, B.; Zhang, D.; Hwang, S.; Wu, C.; Cai, W.X.; Hozain, M.; Kang, W.; Kiedaisch, B.; Strauss, R.E.; Hequet, E.F.; Wyatt, B.G.; Jividen, G.M.; Holaday, A.S.
Transgenic cotton over-producing spinach sucrose phosphate synthase showed enhanced leaf sucrose synthesis and improved fiber quality under controlled environmental conditions
Plant Mol. Biol.
63
815-832
2007
Spinacia oleracea (P31928), Spinacia oleracea
Manually annotated by BRENDA team