Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.14 extracted from

  • Harbron, S.; Foyer, C.; Walker, D.
    The purification and properties of sucrose-phosphate synthetase from spinach leaves: the involvement of this enzyme and fructose bisphosphatase in the regulation of sucrose biosynthesis (1981), Arch. Biochem. Biophys., 212, 237-246.
    View publication on PubMed

General Stability

General Stability Organism
sensitive to freezing and thawing Spinacia oleracea

Inhibitors

Inhibitors Comment Organism Structure
ADP
-
Spinacia oleracea
ATP
-
Spinacia oleracea
fructose-1,6-bisphosphate
-
Spinacia oleracea
phosphate
-
Spinacia oleracea
UDP
-
Spinacia oleracea
UDP-glucose
-
Spinacia oleracea
UTP
-
Spinacia oleracea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.8
-
D-fructose 6-phosphate
-
Spinacia oleracea
7.1
-
UDP-glucose
-
Spinacia oleracea

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ no effect Spinacia oleracea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
270000 280000 gel filtration Spinacia oleracea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-alpha-D-glucose + D-fructose 6-phosphate Spinacia oleracea catalyzes the penultimate step of sucrose synthesis UDP + sucrose 6-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Spinacia oleracea
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Spinacia oleracea

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Spinacia oleracea
-

Storage Stability

Storage Stability Organism
4°C, stable for at least 1 week Spinacia oleracea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-alpha-D-glucose + D-fructose 6-phosphate ordered mechanism, highly specific for its substrates Spinacia oleracea UDP + sucrose 6-phosphate
-
?
UDP-alpha-D-glucose + D-fructose 6-phosphate catalyzes the penultimate step of sucrose synthesis Spinacia oleracea UDP + sucrose 6-phosphate
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.9
-
-
Spinacia oleracea

pH Range

pH Minimum pH Maximum Comment Organism
6 8
-
Spinacia oleracea

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.7
-
UDP
-
Spinacia oleracea
0.8
-
fructose-1,6-bisphosphate
-
Spinacia oleracea
9.4
-
UDP-glucose
-
Spinacia oleracea
11
-
phosphate
-
Spinacia oleracea