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Literature summary for 2.4.1.14 extracted from

  • Sonnewald, U.; Quick, W.P.; MacRae, E.; Krause, K.P.; Stitt, M.
    Purification, cloning and expression of spinach leaf sucrose-phosphate synthase in Escherichia coli (1993), Planta, 189, 174-181.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli and tobacco, active enzyme Spinacia oleracea

Inhibitors

Inhibitors Comment Organism Structure
phosphate E. coli expressed enzyme shows little sensitivity to phosphate inhibition, whereas tobacco expressed enzyme is highly phosphate sensitive Spinacia oleracea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
117600
-
calculated from DNA sequence Spinacia oleracea
120000
-
SDS-PAGE Spinacia oleracea
240000
-
native PAGE Spinacia oleracea
480000
-
gel filtration Spinacia oleracea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-alpha-D-glucose + D-fructose 6-phosphate Spinacia oleracea catalyzes the penultimate step of sucrose synthesis UDP + sucrose 6-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Spinacia oleracea
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Spinacia oleracea

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Spinacia oleracea
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
79.5
-
purified enzyme Spinacia oleracea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-alpha-D-glucose + D-fructose 6-phosphate
-
Spinacia oleracea UDP + sucrose 6-phosphate
-
?
UDP-alpha-D-glucose + D-fructose 6-phosphate catalyzes the penultimate step of sucrose synthesis Spinacia oleracea UDP + sucrose 6-phosphate
-
?