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Literature summary for 2.4.1.14 extracted from

  • Doehlert, D.C.; Huber, S.C.
    Spinach leaf sucrose phosphate snthase. Activation by glucose 6-phosphate and interaction with inorganic phosphate (1983), FEBS Lett., 153, 293-297.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
1,5-Anhydroglucitol 6-phosphate
-
Spinacia oleracea
glucose-6-phosphate reduces Km of fructose-6-phosphate, suggested to be a metabolic regulator in vivo Spinacia oleracea

General Stability

General Stability Organism
20% ethylene glycol and 0.2 M KCl stabilize activity Spinacia oleracea

Inhibitors

Inhibitors Comment Organism Structure
phosphate in absence of glucose-6-phosphate, suggested to be a metabolic regulator in vivo Spinacia oleracea
sucrose-6-phosphate
-
Spinacia oleracea
UDP
-
Spinacia oleracea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4
-
D-fructose 6-phosphate in presence of 2.5 mM glucose 6-phosphate Spinacia oleracea
1.9
-
UDP-glucose in presence and absence of glucose 6-phosphate Spinacia oleracea
4.1
-
D-fructose 6-phosphate in the absence of glucose 6-phosphate Spinacia oleracea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-alpha-D-glucose + D-fructose 6-phosphate Spinacia oleracea catalyzes the penultimate step of sucrose synthesis UDP + sucrose 6-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Spinacia oleracea
-
-
-

Purification (Commentary)

Purification (Comment) Organism
gel filtration is carried out in presence of 20% ethylene glycol and 0.2 M KCl to stabilize activity Spinacia oleracea
partial Spinacia oleracea

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Spinacia oleracea
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-alpha-D-glucose + D-fructose 6-phosphate
-
Spinacia oleracea UDP + sucrose 6-phosphate
-
?
UDP-alpha-D-glucose + D-fructose 6-phosphate catalyzes the penultimate step of sucrose synthesis Spinacia oleracea UDP + sucrose 6-phosphate
-
?