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Literature summary for 2.4.1.14 extracted from
- Regulation of spinach leaf sucrose phosphate synthase by glucose-6-phosphate, inorganic phosphate and pH (1983), Plant Physiol., 73, 989-994.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| D-fructose-1-phosphate | slight activation | Spinacia oleracea |  |
| D-glucose-1-phosphate | slight activation | Spinacia oleracea | |
| glucose-6-phosphate | - |
Spinacia oleracea | |
General Stability
| General Stability | Organism |
|---|---|
| 20% ethylene glycol and 0.2 M KCl stabilize activity | Spinacia oleracea |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| diphosphate | slight inhibition | Spinacia oleracea | |
| NaF | strong inhibition at 20 mM | Spinacia oleracea | |
| phosphate | in absence of glucose-6-phosphate, no inhibition at pH 5.5, inhibitory at alkaline pH | Spinacia oleracea | |
| phosphoenolpyruvate | slight inhibition | Spinacia oleracea | |
| UDP | - |
Spinacia oleracea | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.7 | - |
D-fructose 6-phosphate | in presence of 5 mM glucose 6-phosphate | Spinacia oleracea | |
| 3.2 | - |
D-fructose 6-phosphate | in the absence of glucose 6-phosphate | Spinacia oleracea | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 460000 | - |
gel filtration | Spinacia oleracea |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-alpha-D-glucose + D-fructose 6-phosphate | Spinacia oleracea | catalyzes the penultimate step of sucrose synthesis | UDP + sucrose 6-phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Spinacia oleracea | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| gel filtration is carried out in presence of 20% ethylene glycol and 0.2 M KCl to stabilize activity | Spinacia oleracea |
| partial | Spinacia oleracea |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Spinacia oleracea | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-alpha-D-glucose + D-fructose 6-phosphate | - |
Spinacia oleracea | UDP + sucrose 6-phosphate | - |
? | |
| UDP-alpha-D-glucose + D-fructose 6-phosphate | catalyzes the penultimate step of sucrose synthesis | Spinacia oleracea | UDP + sucrose 6-phosphate | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
broad optimum | Spinacia oleracea |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 9 | - |
Spinacia oleracea |
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