Activating Compound | Comment | Organism | Structure |
---|---|---|---|
glucose-6-phosphate | - |
Spinacia oleracea | |
glucose-6-phosphate | - |
Prosopis juliflora | |
Mg2+ | stimulates enzyme activity | Spinacia oleracea | |
Mg2+ | stimulates enzyme activity | Prosopis juliflora | |
Mn2+ | stimulates enzyme activity | Prosopis juliflora |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | no inhibition | Prosopis juliflora | |
ADP | - |
Spinacia oleracea | |
ATP | reversible by addition of Mg2+ | Prosopis juliflora | |
ATP | - |
Spinacia oleracea | |
CTP | reversible by addition of Mg2+ | Prosopis juliflora | |
fluoride | strong inhibition | Prosopis juliflora | |
fluoride | - |
Spinacia oleracea | |
GTP | reversible by addition of Mg2+ | Prosopis juliflora | |
ITP | reversible by addition of Mg2+ | Prosopis juliflora | |
p-chloromercuribenzoate | activity can be restored by addition of DTT or 2-mercaptoethanol | Prosopis juliflora | |
phosphate | competitive with UDP-glucose | Prosopis juliflora | |
phosphate | competitive with UDP-glucose | Spinacia oleracea | |
sulfate | - |
Prosopis juliflora | |
sulfate | - |
Spinacia oleracea | |
UDP | competitive with UDP-glucose | Prosopis juliflora | |
UDP | - |
Spinacia oleracea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | - |
D-fructose 6-phosphate | Km is not effected by addition of 20 mM glucose 6-phosphate or 15 mM phosphate | Prosopis juliflora | |
4.6 | - |
UDP-glucose | in presence of 20 mM glucose 6-phosphate | Prosopis juliflora | |
20 | - |
UDP-glucose | - |
Prosopis juliflora | |
77 | - |
UDP-glucose | in presence of 15 mM phosphate | Prosopis juliflora |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | stimulates enzyme activity | Spinacia oleracea | |
Mg2+ | stimulates enzyme activity | Prosopis juliflora | |
Mn2+ | - |
Spinacia oleracea | |
Mn2+ | stimulates enzyme activity | Prosopis juliflora |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
443000 | - |
gel filtration | Prosopis juliflora |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-glucose + D-fructose 6-phosphate | Spinacia oleracea | catalyzes the penultimate step of sucrose synthesis | UDP + sucrose 6-phosphate | - |
? | |
UDP-alpha-D-glucose + D-fructose 6-phosphate | Prosopis juliflora | catalyzes the penultimate step of sucrose synthesis | UDP + sucrose 6-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Prosopis juliflora | - |
- |
- |
Spinacia oleracea | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial, at least 3 additional proteins could not be removed | Prosopis juliflora |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Spinacia oleracea | - |
leaf | - |
Prosopis juliflora | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
28 | - |
purified enzyme, specific activity in crude extract is extremely low compared to spinach | Prosopis juliflora |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-glucose + D-fructose 6-phosphate | - |
Spinacia oleracea | UDP + sucrose 6-phosphate | - |
? | |
UDP-alpha-D-glucose + D-fructose 6-phosphate | ordered bi-bi mechanism | Prosopis juliflora | UDP + sucrose 6-phosphate | - |
? | |
UDP-alpha-D-glucose + D-fructose 6-phosphate | catalyzes the penultimate step of sucrose synthesis | Spinacia oleracea | UDP + sucrose 6-phosphate | - |
? | |
UDP-alpha-D-glucose + D-fructose 6-phosphate | catalyzes the penultimate step of sucrose synthesis | Prosopis juliflora | UDP + sucrose 6-phosphate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Prosopis juliflora |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Prosopis juliflora |