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Enzyme Nomenclature
Information on EC 2.4.1.14 - sucrose-phosphate synthase
Word Map on EC 2.4.1.14
- 2.4.1.14
- leaf
-
photosynthetic
- starch
- invertase
- maize
-
sink
-
pyrophosphorylase
- oleracea
-
spinacia
-
diurnal
- fructose-1,6-bisphosphatase
-
photosynthate
- ribulose-1,5-bisphosphate
- rubisco
- sugarcane
- adp-glucose
-
midday
-
2,6-bisphosphate
- stachyose
- saccharum
- fructose-2,6-bisphosphate
-
sucrose-metabolizing
-
3.2.1.26
-
fructans
- analysis
- diagnostics
- agriculture
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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
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EC NUMBER 
COMMENTARY 
2.4.1.14
-
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RECOMMENDED NAME
GeneOntology No.
sucrose-phosphate synthase
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
UDP-glucose + D-fructose 6-phosphate = UDP + sucrose 6F-phosphate
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hexosyl group transfer
-
-
-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
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SYSTEMATIC NAME
IUBMB Comments
UDP-glucose:D-fructose-6-phosphate 2-alpha-D-glucosyltransferase
Requires Mg2+ or Mn2+ for maximal activity [2]. The enzyme from Synechocystis sp. strain PCC 6803 is not specific for UDP-glucose as it can use ADP-glucose and, to a lesser extent, GDP-glucose as substrates [2]. The enzyme from rice leaves is activated by glucose 6-phosphate but that from cyanobacterial species is not [2]. While the reaction catalysed by this enzyme is reversible, the enzyme usually works in concert with EC 3.1.3.24, sucrose-phosphate phosphatase, to form sucrose, making the above reaction essentially irreversible [3]. The F in sucrose 6F-phosphate is used to indicate that the fructose residue of sucrose carries the substituent.
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CAS REGISTRY NUMBER
COMMENTARY
9030-06-2
-
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
cv. Mesa, plants inoculated with Sinorhizobium meliloti, three different isoforms of SPS belonging to two dfferent SPS gene families, SPSA and SPSB, in Medicago sativa
Uniprot
high sucrose accumulating cultivars CoS96268 and CoS95255, and late maturing, low sucrose accumulating cultivars CoS97264, CoSe92423
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-
six Indian potato cultivars namely Kufri Chipsona-1, Kufri Chipsona-2, Kufri Chandramukhi, Kufri Jyoti, Kufri Ashoka, and Kufri Pukhraj, gene KC-SPS1
UniProt
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
evolution
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a comparison of the sequences of the wheat SPSII orthologues present in the diploid progenitors Triticum monococcum, Triticum urartu, Triticum speltoides, Aegilops tauschii, and Triticum speltoides, as well as in the more distantly related species Hordeum vulgare, Oryza sativa, Sorghum and purple false brome, Brachypodium distachyon, demonstrates that intronic sequence is less well conserved than exonic. Comparative sequence and phylogenetic analysis of SPSII gene shows that false purple brome is more similar to Triticeae than to Oryza sativa
malfunction
metabolism
physiological function
additional information
malfunction
an spsa1 knock-out mutant shows a 44% decrease in leaf enzyme activity compared to the wild-type and a slight increase in leaf starch content at the end of the light period as well as at the end of the dark period. An spsa1/spsc double mutant is strongly impaired in growth and accumulated high levels of starch. This increase in starch is probably not due to an increased partitioning of carbon into starch, but is rather caused by an impaired starch mobilization during the night. Sucrose export from excised petioles harvested from spsa1/spsc double mutant plants is significantly reduced under illumination as well as during the dark period. Loss of the two major SPS isoforms in leaves limits Suc synthesis without grossly changingcarbon partitioning in favour of starch during the light period but limits starch degradation during the dark period; the spsc null mutant displays reduced sucrose contents towards the end of the photoperiod and a concomitant 25% reduction in enzyme activity. In contrast, an spsa1/spsc double mutant is strongly impaired in growth and accumulated high levels of starch. This increase in starch is probably not due to an increased partitioning of carbon into starch, but is rather caused by an impaired starch mobilization during the night. Sucrose export from excised petioles harvested from spsa1/spsc double mutant plants is significantly reduced under illumination as well as during the dark period. Loss of the two major SPS isoforms in leaves limits Suc synthesis without grossly changing carbon partitioning in favour of starch during the light period but limits starch degradation during the dark period
malfunction
mutation of AtSWEET9 or nectary-expressed sucrose phosphate synthase genes leads to the loss of nectar secretion; mutation of AtSWEET9 or nectary-expressed sucrose phosphate synthase genes leads to the loss of nectar secretion
malfunction
mutation of AtSWEET9 or nectary-expressed sucrose phosphate synthase genes leads to the loss of nectar secretion
malfunction
-
mutation of AtSWEET9 or nectary-expressed sucrose phosphate synthase genes leads to the loss of nectar secretion
malfunction
overexpression and increased activity of the enzyme in alfalfa is accompanied by early flowering, increased plant growth and an increase in elemental N and protein content when grown under N2-fixing conditions
metabolism
SPS catalyzes the first step in the synthesis of sucrose in photosynthetic tissue
metabolism
-
sucrose phosphate synthase, together with the soluble acid invertase, are the key enzymes in regulating sucrose accumulation in sugarcane stalk, overview
metabolism
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sucrose phosphate synthase is an important component of the plant sucrose biosynthesis pathway
metabolism
-
sucrose phosphate synthase and sucrose synthase, EC 2.4.1.13, are key enzymes in the synthesis and breakdown of sucrose in sugarcane
metabolism
the sucrose phosphate synthase reaction is the key enzymatic step in sucrose synthesis in plants. Sucrose phosphate phosphatase and sucrose phosphate synthase catalyze sequential reactions in sucrose synthesis in green plant cells, the interaction between decreased sucrose phosphate phosphatase activity and sucrose phosphate synthase activity may alter sucrose synthesis during cold acclimation in Klebsormidium flaccidum, enzyme regulation, overview
metabolism
the enzyme SPS is encoded by different gene families. SPS exists in multiple forms which show differential distributions and functional specializations in the plant tissues. SPS activity is highly regulated by hierarchy of mechanisms including transcriptional control
metabolism
sucrose-phosphate synthase catalyses one of the rate-limiting steps in the synthesis of sucrose in plants; sucrose-phosphate synthase catalyses one of the rate-limiting steps in the synthesis of sucrose in plants; sucrose-phosphate synthase catalyses one of the rate-limiting steps in the synthesis of sucrose in plants; sucrose-phosphate synthase catalyses one of the rate-limiting steps in the synthesis of sucrose in plants
metabolism
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relationship between the contents of carbohydrate and sucrose metabolizing enzymes activities, overview
metabolism
relationship between the contents of carbohydrate and sucrose-metabolizing enzymes activities, overview
metabolism
sucrose phosphate synthase is a key enzyme for sucrose biosynthesis; sucrose phosphate synthase is a key enzyme for sucrose biosynthesis
metabolism
sucrose phosphate synthase is a key enzyme for sucrose biosynthesis
metabolism
-
sucrose phosphate synthase is a key enzyme for sucrose biosynthesis
metabolism
the enzyme plays a key role in carbon metabolism by catalyzing the synthesis of sucrose
physiological function
-
SPS plays a crucial role in carbohydrate metabolism by regulating the partitioning of carbon between starch production and carbohydrate accumulation in many physiological and developmental processes, including responses to water stress, diurnal carbohydrate allocation within plants, and fruit and flower development
physiological function
-
molecular mechanism of transcriptional regulation of banana sucrose phosphate synthase gene during fruit ripening by functions of various cis-acting regulatory elements, overview. Presence of specific trans-acting factors which showed specific interactions with ethylene, auxin, low temperature and light responsive elements in regulating SPS transcription
physiological function
sucrose-phosphate synthase refers to a key enzyme in sucrose biosynthesis in both photosynthetic and nonphotosynthetic tissues of plants
physiological function
the enzyme is highly expressed in nectaries and that their expression is also essential for nectar secretion. Sucrose is synthesized in the nectary parenchyma by the enzyme and subsequently secreted into the extracellular space via SWEET9,a nectary-specific sugar transporter.In the the extracellular, sucrose is hydrolysed by an apoplasmic invertase to produce a mixture of sucrose, glucose and fructose. SWEET9 is essential for nectar production and can function as an efflux transporter; the enzyme is highly expressed in nectaries, the expression is essential for nectar secretion. Sucrose is synthesized in the nectary parenchyma by the enzyme and subsequently secreted into the extracellular space via SWEET9, a nectary-specific sugar transporter. In the extracellular, sucrose is hydrolysed by an apoplasmic invertase to produce a mixture of sucrose, glucose and fructose. SWEET9 is essential for nectar production and can function as an efflux transporter
physiological function
the enzyme is highly expressed in nectaries, the expression is essential for nectar secretion. Sucrose is synthesized in the nectary parenchyma by the enzyme and subsequently secreted into the extracellular space via SWEET9, a nectary-specific sugar transporter. In the extracellular, sucrose is hydrolysed by an apoplasmic invertase to produce a mixture of sucrose, glucose and fructose. SWEET9 is essential for nectar production and can function as an efflux transporter
physiological function
-
the enzyme is highly expressed in nectaries, the expression is essential for nectar secretion. Sucrose is synthesized in the nectary parenchyma by the enzyme and subsequently secreted into the extracellular space via SWEET9, a nectary-specific sugar transporter. In the extracellular, sucrose is hydrolysed by an apoplasmic invertase to produce a mixture of sucrose, glucose and fructose. SWEET9 is essential for nectar production and can function as an efflux transporter
physiological function
the enzyme is essential for plant viability, the four SPS isozymes function in processes that are important for plant growth and nonstructural carbohydrate metabolism
additional information
-
gene expression analysis of sucrose biosynthesis genes during wheat plant ontogeny, overview
additional information
expression of all the SPS genes, particularly that of SPS1 and SPS11, tends to be higher at night when the activation state of the SPS proteins is low, and the mRNA levels of SPS1 and SPS6 are negatively correlated with sucrose content. The temporal patterns of SPS gene expression and sugar content under continuous light conditions suggest the involvement of endogenous rhythm and/or sucrose sensing in the transcriptional regulation of SPS genes, overview; expression of all the SPS genes, particularly that of SPS1 and SPS11, tends to be higher at night when the activation state of the SPS proteins is low, and the mRNA levels of SPS1 and SPS6 are negatively correlated with sucrose content. The temporal patterns of SPS gene expression and sugar content under continuous light conditions suggest the involvement of endogenous rhythm and/or sucrose sensing in the transcriptional regulation of SPS genes, overview; expression of all the SPS genes, particularly that of SPS1 and SPS11, tends to be higher at night when the activation state of the SPS proteins is low, and the mRNA levels of SPS1 and SPS6 are negatively correlated with sucrose content. The temporal patterns of SPS gene expression and sugar content under continuous light conditions suggest the involvement of endogenous rhythm and/or sucrose sensing in the transcriptional regulation of SPS genes, overview; expression of all the SPS genes, particularly that of SPS1 and SPS11, tends to be higher at night when the activation state of the SPS proteins is low, and the mRNA levels of SPS1 and SPS6 are negatively correlated with sucrose content. The temporal patterns of SPS gene expression and sugar content under continuous light conditions suggest the involvement of endogenous rhythm and/or sucrose sensing in the transcriptional regulation of SPS genes, overview; expression of all the SPS genes, particularly that of SPS1 and SPS11, tends to be higher at night when the activation state of the SPS proteins is low, and the mRNA levels of SPS1 and SPS6 are negatively correlated with sucrose content. The temporal patterns of SPS gene expression and sugar content under continuous light conditions suggest the involvement of endogenous rhythm and/or sucrose sensing in the transcriptional regulation of SPS genes, overview
additional information
expression of all the SPS genes, particularly that of SPS1 and SPS11, tends to be higher at night when the activation state of the SPS proteins is low, and the mRNA levels of SPS1 and SPS6 are negatively correlated with sucrose content. The temporal patterns of SPS gene expression and sugar content under continuous light conditions suggest the involvement of endogenous rhythm and/or sucrose sensing in the transcriptional regulation of SPS genes, overview; expression of all the SPS genes, particularly that of SPS1 and SPS11, tends to be higher at night when the activation state of the SPS proteins is low, and the mRNA levels of SPS1 and SPS6 are negatively correlated with sucrose content. The temporal patterns of SPS gene expression and sugar content under continuous light conditions suggest the involvement of endogenous rhythm and/or sucrose sensing in the transcriptional regulation of SPS genes, overview; expression of all the SPS genes, particularly that of SPS1 and SPS11, tends to be higher at night when the activation state of the SPS proteins is low, and the mRNA levels of SPS1 and SPS6 are negatively correlated with sucrose content. The temporal patterns of SPS gene expression and sugar content under continuous light conditions suggest the involvement of endogenous rhythm and/or sucrose sensing in the transcriptional regulation of SPS genes, overview; expression of all the SPS genes, particularly that of SPS1 and SPS11, tends to be higher at night when the activation state of the SPS proteins is low, and the mRNA levels of SPS1 and SPS6 are negatively correlated with sucrose content. The temporal patterns of SPS gene expression and sugar content under continuous light conditions suggest the involvement of endogenous rhythm and/or sucrose sensing in the transcriptional regulation of SPS genes, overview; expression of all the SPS genes, particularly that of SPS1 and SPS11, tends to be higher at night when the activation state of the SPS proteins is low, and the mRNA levels of SPS1 and SPS6 are negatively correlated with sucrose content. The temporal patterns of SPS gene expression and sugar content under continuous light conditions suggest the involvement of endogenous rhythm and/or sucrose sensing in the transcriptional regulation of SPS genes, overview
additional information
expression of all the SPS genes, particularly that of SPS1 and SPS11, tends to be higher at night when the activation state of the SPS proteins is low, and the mRNA levels of SPS1 and SPS6 are negatively correlated with sucrose content. The temporal patterns of SPS gene expression and sugar content under continuous light conditions suggest the involvement of endogenous rhythm and/or sucrose sensing in the transcriptional regulation of SPS genes, overview; expression of all the SPS genes, particularly that of SPS1 and SPS11, tends to be higher at night when the activation state of the SPS proteins is low, and the mRNA levels of SPS1 and SPS6 are negatively correlated with sucrose content. The temporal patterns of SPS gene expression and sugar content under continuous light conditions suggest the involvement of endogenous rhythm and/or sucrose sensing in the transcriptional regulation of SPS genes, overview; expression of all the SPS genes, particularly that of SPS1 and SPS11, tends to be higher at night when the activation state of the SPS proteins is low, and the mRNA levels of SPS1 and SPS6 are negatively correlated with sucrose content. The temporal patterns of SPS gene expression and sugar content under continuous light conditions suggest the involvement of endogenous rhythm and/or sucrose sensing in the transcriptional regulation of SPS genes, overview; expression of all the SPS genes, particularly that of SPS1 and SPS11, tends to be higher at night when the activation state of the SPS proteins is low, and the mRNA levels of SPS1 and SPS6 are negatively correlated with sucrose content. The temporal patterns of SPS gene expression and sugar content under continuous light conditions suggest the involvement of endogenous rhythm and/or sucrose sensing in the transcriptional regulation of SPS genes, overview; expression of all the SPS genes, particularly that of SPS1 and SPS11, tends to be higher at night when the activation state of the SPS proteins is low, and the mRNA levels of SPS1 and SPS6 are negatively correlated with sucrose content. The temporal patterns of SPS gene expression and sugar content under continuous light conditions suggest the involvement of endogenous rhythm and/or sucrose sensing in the transcriptional regulation of SPS genes, overview
additional information
expression of all the SPS genes, particularly that of SPS1 and SPS11, tends to be higher at night when the activation state of the SPS proteins is low, and the mRNA levels of SPS1 and SPS6 are negatively correlated with sucrose content. The temporal patterns of SPS gene expression and sugar content under continuous light conditions suggest the involvement of endogenous rhythm and/or sucrose sensing in the transcriptional regulation of SPS genes, overview; expression of all the SPS genes, particularly that of SPS1 and SPS11, tends to be higher at night when the activation state of the SPS proteins is low, and the mRNA levels of SPS1 and SPS6 are negatively correlated with sucrose content. The temporal patterns of SPS gene expression and sugar content under continuous light conditions suggest the involvement of endogenous rhythm and/or sucrose sensing in the transcriptional regulation of SPS genes, overview; expression of all the SPS genes, particularly that of SPS1 and SPS11, tends to be higher at night when the activation state of the SPS proteins is low, and the mRNA levels of SPS1 and SPS6 are negatively correlated with sucrose content. The temporal patterns of SPS gene expression and sugar content under continuous light conditions suggest the involvement of endogenous rhythm and/or sucrose sensing in the transcriptional regulation of SPS genes, overview; expression of all the SPS genes, particularly that of SPS1 and SPS11, tends to be higher at night when the activation state of the SPS proteins is low, and the mRNA levels of SPS1 and SPS6 are negatively correlated with sucrose content. The temporal patterns of SPS gene expression and sugar content under continuous light conditions suggest the involvement of endogenous rhythm and/or sucrose sensing in the transcriptional regulation of SPS genes, overview; expression of all the SPS genes, particularly that of SPS1 and SPS11, tends to be higher at night when the activation state of the SPS proteins is low, and the mRNA levels of SPS1 and SPS6 are negatively correlated with sucrose content. The temporal patterns of SPS gene expression and sugar content under continuous light conditions suggest the involvement of endogenous rhythm and/or sucrose sensing in the transcriptional regulation of SPS genes, overview
additional information
expression of all the SPS genes, particularly that of SPS1 and SPS11, tends to be higher at night when the activation state of the SPS proteins is low, and the mRNA levels of SPS1 and SPS6 are negatively correlated with sucrose content. The temporal patterns of SPS gene expression and sugar content under continuous light conditions suggest the involvement of endogenous rhythm and/or sucrose sensing in the transcriptional regulation of SPS genes, overview; expression of all the SPS genes, particularly that of SPS1 and SPS11, tends to be higher at night when the activation state of the SPS proteins is low, and the mRNA levels of SPS1 and SPS6 are negatively correlated with sucrose content. The temporal patterns of SPS gene expression and sugar content under continuous light conditions suggest the involvement of endogenous rhythm and/or sucrose sensing in the transcriptional regulation of SPS genes, overview; expression of all the SPS genes, particularly that of SPS1 and SPS11, tends to be higher at night when the activation state of the SPS proteins is low, and the mRNA levels of SPS1 and SPS6 are negatively correlated with sucrose content. The temporal patterns of SPS gene expression and sugar content under continuous light conditions suggest the involvement of endogenous rhythm and/or sucrose sensing in the transcriptional regulation of SPS genes, overview; expression of all the SPS genes, particularly that of SPS1 and SPS11, tends to be higher at night when the activation state of the SPS proteins is low, and the mRNA levels of SPS1 and SPS6 are negatively correlated with sucrose content. The temporal patterns of SPS gene expression and sugar content under continuous light conditions suggest the involvement of endogenous rhythm and/or sucrose sensing in the transcriptional regulation of SPS genes, overview; expression of all the SPS genes, particularly that of SPS1 and SPS11, tends to be higher at night when the activation state of the SPS proteins is low, and the mRNA levels of SPS1 and SPS6 are negatively correlated with sucrose content. The temporal patterns of SPS gene expression and sugar content under continuous light conditions suggest the involvement of endogenous rhythm and/or sucrose sensing in the transcriptional regulation of SPS genes, overview
additional information
most of the potato cultivars are autotetraploid (2n = 4x = 48), highly heterozygous, and show high level of DNA polymorphism in its genome. Natural allelic variations are also common in potato genes
additional information
the N-terminal region of sugarcane sucrose phosphate synthase is not essential for the catalytic reaction itself, but is crucial for the allosteric regulation by glucose 6-phosphate and may function like a suppressor domain for the enzyme activity
additional information
sucrose phosphate synthase contains a putative C-terminal sucrose phosphate phosphatase-like domain that may facilitates the binding of sucrose phosphate phosphatase, EC 3.1.3.24, interaction analysis in transgenic plants and yeast two-hybrid system, overview
additional information
-
sucrose phosphate synthase contains a putative C-terminal sucrose phosphate phosphatase-like domain that may facilitates the binding of sucrose phosphate phosphatase, EC 3.1.3.24, interaction analysis in transgenic plants and yeast two-hybrid system, overview
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
UDP + sucrose 6F-phosphate
UDP-glucose + D-fructose 6-phosphate
-
-
-
r
ADP-glucose + D-fructose 6-phosphate
ADP + sucrose 6-phosphate
-
only SPS-I
-
?
ADP-glucose + D-fructose 6-phosphate
ADP + sucrose 6-phosphate
-
only SPS-I
-
?
GDP-glucose + D-fructose 6-phosphate
GDP + sucrose 6-phosphate
-
only SPS-I
-
?
GDP-glucose + D-fructose 6-phosphate
GDP + sucrose 6-phosphate
-
only SPS-I
-
?
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
-
-
r
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
-
-
-
?
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
high SPS activity in cold long days leading to hyper accumulation of sucrose appears to be among the features that permit Deschampsia antarctica to survive in the harsh Antarctic conditions
-
-
?
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
-
-
r
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
-
-
r
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
supports synthesis of secondary wall cellulose by releasing UDP-glucose from sucrose
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
-
-
-
?
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
gene expression is minimal without illumination
-
-
?
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
-
-
?
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
-
-
r
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
-
-
?
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
-
-
r
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
supports synthesis of secondary wall cellulose by releasing UDP-glucose from sucrose
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
upregulation of the enzyme under elevated CO2 plus temperature
-
-
?
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
ordered bi-bi mechanism
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
involved in regulation of carbon partitioning in leaves
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
-
-
-
?
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
-
-
r
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
-
-
r
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
-
-
r
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
ordered mechanism, highly specific for its substrates
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
catalyzes the penultimate step of sucrose synthesis
638626, 638634, 638635, 638636, 638637, 638638, 638639, 638640, 638641, 638643, 638645, 638646, 638647, 638649, 638650, 638651, 638652, 638654, 638657
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
involved in regulation of carbon partitioning in leaves
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
-
-
-
?
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
-
-
-
?
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
-
-
r
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
-
equilibrium lies far on the product side
r
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
ordered mechanism with UDP-glucose as first substrate bound and UDP as last product released
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
key enzyme of sucrose synthesis
-
-
?
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
-
-
-
?
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
-
-
r
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
involved in regulation of carbon partitioning in leaves
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
overexpression of maize sucrose-phosphate synthase gene in Nicotiana tabacum increases the sucrose synthesis and carbon assimilation, particularly in older leaves, accelerates the whole plant development and increases the abundance of flowers without substantial changes in the overall shoot biomass
-
-
?
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
-
-
r
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
supports synthesis of secondary wall cellulose by releasing UDP-glucose from sucrose
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6F-phosphate
-
-
-
-
?
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6F-phosphate
-
-
-
r
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6F-phosphate
-
-
-
?
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6F-phosphate
-
-
-
?
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6F-phosphate
-
-
-
?
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6F-phosphate
-
-
-
r
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6F-phosphate
-
-
-
?
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6F-phosphate
isozyme show differences in allosteric regulation
-
-
?
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6F-phosphate
optimal substrate concentrations are 10 mM for D-fructose 6-phosphate and 12 mM for UDP-glucose
-
-
?
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6F-phosphate
-
-
-
r
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6F-phosphate
-
-
-
-
r
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6F-phosphate
-
-
-
-
?
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6F-phosphate
-
-
-
?
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6F-phosphate
-
-
-
-
?
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6F-phosphate
-
-
-
?
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6F-phosphate
-
-
-
?
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6F-phosphate
-
-
-
?
additional information
?
-
a bifunctional sucrose phosphate synthase/phosphatase (SPS/SPP)
-
-
-
additional information
?
-
-
fructose or fructose 1-phosphate are not accepted as substrates
-
-
-
additional information
?
-
-
development of homoeologue-specific assays, overview
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
additional information
?
-
Q1GY13
a bifunctional sucrose phosphate synthase/phosphatase (SPS/SPP)
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
high SPS activity in cold long days leading to hyper accumulation of sucrose appears to be among the features that permit Deschampsia antarctica to survive in the harsh Antarctic conditions
-
-
?
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
supports synthesis of secondary wall cellulose by releasing UDP-glucose from sucrose
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
gene expression is minimal without illumination
-
-
?
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
G1UJV3
-
-
-
?
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
B7F7B9, Q0JGK4, Q53JI9, Q67WN8, Q6ZHZ1
-
-
-
?
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
supports synthesis of secondary wall cellulose by releasing UDP-glucose from sucrose
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
upregulation of the enzyme under elevated CO2 plus temperature
-
-
?
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
involved in regulation of carbon partitioning in leaves
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
-
-
-
?
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
catalyzes the penultimate step of sucrose synthesis
638626, 638634, 638635, 638636, 638637, 638638, 638639, 638640, 638641, 638643, 638645, 638646, 638647, 638649, 638650, 638651, 638652, 638654, 638657
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
involved in regulation of carbon partitioning in leaves
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
-
-
-
?
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
-
-
-
?
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
key enzyme of sucrose synthesis
-
-
?
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
involved in regulation of carbon partitioning in leaves
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
overexpression of maize sucrose-phosphate synthase gene in Nicotiana tabacum increases the sucrose synthesis and carbon assimilation, particularly in older leaves, accelerates the whole plant development and increases the abundance of flowers without substantial changes in the overall shoot biomass
-
-
?
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-alpha-D-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
-
supports synthesis of secondary wall cellulose by releasing UDP-glucose from sucrose
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6F-phosphate
-
-
-
-
?
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6F-phosphate
Q94BT0
-
-
-
r
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6F-phosphate
F4JLK2, Q8RY24, Q94BT0, Q9FY54
-
-
-
?
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6F-phosphate
Q94BT0
-
-
-
?
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6F-phosphate
Q94BT0
-
-
-
?
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6F-phosphate
Q84XS4
-
-
-
r
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6F-phosphate
Q9AXK3
-
-
-
?
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6F-phosphate
Q9AXK3
isozyme show differences in allosteric regulation
-
-
?
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6F-phosphate
Q1GY13
-
-
-
r
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6F-phosphate
-
-
-
-
r
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6F-phosphate
-
-
-
-
?
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6F-phosphate
P93782
-
-
-
?
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6F-phosphate
-
-
-
-
?
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6F-phosphate
Q9FXK8
-
-
-
?
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6F-phosphate
B2ZSP7
-
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Mg2+
Mn2+
Mg2+
required; required; required; required; required
Mg2+
-
reverses inhibition with citrate, phosphate and nucloside triphosphates
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
cycloheximide
-
reduces Vmax to one third of control when supplied to intact leaves, no effect on light activation
cycloheximide
-
no significant effect on Vmax, reduces extent of light activation
p-chloromercuribenzoate
-
activity can be restored by addition of DTT or 2-mercaptoethanol
p-chloromercuribenzoate
-
activity can be restored by addition of DTT or 2-mercaptoethanol
phosphate
86.3% inhibition of the nodule isozyme at 5 mM, 15.7% and 25.9% inhibition of the leaf enzyme at 2 mM and 5 mM, respectively. The inhibitory effect is increased in plants infected with an N-fixation deficient Sinorhizobium meliloti strain
phosphate
-
SPS-I shows high sensitivity towards phosphate, whereas SPS-II is only slightly sensitive
phosphate
-
in absence of glucose-6-phosphate, suggested to be a metabolic regulator in vivo
phosphate
-
in absence of glucose-6-phosphate, no inhibition at pH 5.5, inhibitory at alkaline pH
phosphate
-
partial competitive inhibitor with respect to both substrates in presence of 5 mM glucose-6-phosphate, competes with glucose-6-phosphate for the same binding site
phosphate
-
maximum inhibition with 1.5-2 mol phosphate per mol tetramer, maximum velocity is not affected
phosphate
-
E. coli expressed enzyme shows little sensitivity to phosphate inhibition, whereas tobacco expressed enzyme is highly phosphate sensitive
UDP
-
competitive with UDP-glucose; reversible by addition of 5 mM Mn2+ or 10 mM Mg2+
additional information
poor effect by fructose and sucrose
-
additional information
-
preparation contains a protein kinase that can phosphorylate and therefore inactivate enzyme activity
-
additional information
-
the Synechocystis sps gene is introduced into tobacco, rice and tomato under the control of constitutive promoters. The Synechocystis SPS protein is expressed at high level. However SPS activities and carbon partitioning in leaves from transgenic and wild-type plants are not significantly different. The purified enzymes have full catalytic activity. It is proposed that some other protein in plant cells binds to the Synechocystis SPS resulting in inhibition of the enzyme
-
additional information
-
mechanisms involved in short-term feedback inhibition of sucrose synthesis by sucrose are lacking in wheat
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
benzyladenine
-
the phytohormone increases the specific activity of the enzyme practically during the entire growing period, except for the youngest leaves
D-glucose 6-phosphate
157-165% activation, the activation effect is reduced in plants infected with an N-fixation deficient Sinorhizobium meliloti strain
D-Glucose-6-phosphate
-
the enzyme is activated in light, with an increased affinity for its substrates and the activator glucose-6-phosphate, reduces sensitivity to inhibition by phosphate, but no change in maximal catalytic activity
gibberellic acid
-
the phytohormone increases the specific activity of the enzyme practically during the entire growing period, except for the youngest leaves
glucose 6-phosphate
allosteric activation of the wild-type enzyme, N-terminally truncated enzymes are not activated
IAA
-
the phytohormone increases specific activity of the enzyme practically during the entire growing period, except for the youngest leaves
phosphate
-
stimulating at low concentration particularly at high fructose-6-phosphate concentrations
glucose-6-phosphate
-
reduces Km of fructose-6-phosphate, suggested to be a metabolic regulator in vivo
glucose-6-phosphate
-
two enzyme species differ in their degree of activation
light
-
active, dephosphorylated is formed in light period
-
additional information
-
increase of enzyme activity in leaves during earlier stages of fruit development until 12 days after anthesis, increase of enzyme activity in mesocarp tissue until day 8 after anthesis
-
additional information
-
although SPS activity does not display an endogenous rhythm of activity in continuous light, activation of SPS at the end of the dark period is observed. This activation is possibly controlled by covalent modification, because it is inhibited by okadaic acid while the SPS protein level does not significantly change. The highest SPS activity is observed after 21 days of cold acclimation under long day conditions. This increased activity is not related to an increase in SPS gene expression or protein content
-
additional information
-
recombinant enzyme expressed in Escherichia coli is not activated by glucose 6-phosphate
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.19
sucrose 6F-phosphate
pH 6.5, 45°C, recombinant His6-tagged enzyme
additional information
additional information
allosteric regulation by glucose 6-phosphate
-
0.2
D-fructose 6-phosphate
-
pH 8.0, cosubstrate: UDP-glucose, enzyme expressed in Escherichia coli
0.3
D-fructose 6-phosphate
-
pH 8.0, cosubstrate: UDP-glucose, enzyme expressed in Nicotiana tabacum
1.2
D-fructose 6-phosphate
pH 6.5, 45°C, recombinant His6-tagged enzyme
2
D-fructose 6-phosphate
-
Km is not effected by addition of 20 mM glucose 6-phosphate or 15 mM phosphate
1.3
UDP-glucose
pH 6.5, 45°C, recombinant His6-tagged enzyme, in presence of fructose 1,6-bisphosphate
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.013
-
value about, mesocarp tissue, 16 days after anthesis; value about, mesocarp tissue, 4 days after anthesis
0.0167
-
value about, leaf, 16 days after anthesis; value about, leaf, day of anthesis; value about, mesocarp tissue, 8 days and 12 days after anthesis
0.0316
wild-type leaf isozyme from plants infected with an N-fixation deficient Sinorhizobium meliloti strain
0.0322
wild-type leaf isozyme from plants infected with a wild-type Sinorhizobium meliloti strain
0.0767
wild-type nodule isozyme from plants infected with an N-fixation deficient Sinorhizobium meliloti strain
0.0861
wild-type nodule isozyme from plants infected with a wild-type Sinorhizobium meliloti strain
0.5
purified recombinant enzyme, pH 7.5, temperature not specified in the publication
0.56
purified recombinant enzyme, pH 7.5, temperature not specified in the publication
1.19
purified recombinant enzyme, pH 7.5, temperature not specified in the publication
2.19
purified recombinant enzyme, pH 7.5, temperature not specified in the publication
28
-
purified enzyme, specific activity in crude extract is extremely low compared to spinach
additional information
additional information
-
enzyme activity during fruit development in wild-type and transgenic antisense plants, overview
additional information
-
enzyme activity in banana pulp during fruit ripening, overview
additional information
-
sucrose concentrations in wild-type and transgenic plant leaves, overview
additional information
enzyme activities in different cultivars and tissues, overview
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
6.5
7.5
7.5
assay at; assay at; assay at; assay at
7.5
assay at; assay at; assay at; assay at; assay at
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
4.5 - 9
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
25
37
45
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TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
25 - 55
25 - 55
-
25°C: about 70% of maximal activity, 55°C: about 45% of maximal activity
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pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
-
maximal activity of SPS in illumination grown calli appears on the 14th day after the culture is transferred into a new medium. Very low activity in dark grown cells
-
although the level of SPS mRNA and protein is lower in embryos than in leaf, enzymatic activity is higher
-
the level of SPS transcript is 10fold lower in endosperm than in leaf but the level of SPS protein is comparable and the activity is 2fold higher
high expression level of genes SPS1F and SPS2F in maturing nectaries; high expression level of genes SPS1F and SPS2F in maturing nectaries
additional information
-
ethylene strongly stimulates SPS transcript accumulation, auxin and cold treatment only marginally increase the abundance of SPS mRNA level, while wounding negatively regulates SPS gene expression. SPS transcript level is distinctly increased by constant exposure to white light. Protein level, enzymatic activity of SPS and sucrose synthesis are substantially increased by ethylene and increased exposure to white light conditions as compared to other treatments
-
activity of SPS is significantly higher in the upper internodes of high commercial cane sugar clones as compared with low commercial cane sugar clones in both populations, suggesting that this enzyme may have a key role in establishing metabolic and developmental processes necessary for high sugar accumulation during stem growth and maturation
-
SPS activity and transcript expression is higher in mature internodes compared with immature internodes in all the studied cultivars
-
enzyme activity in young intermodes of different cultivars, overview
determination of sucrose efflux from source leaves; young and mature, determination of sucrose efflux from source leaves; young and mature, isozyme AtSPSA1 is one of the major isoforms expressed in leaves. Determination of sucrose efflux from source leaves. AtSPSC constitutive expression is about 75% lower than that of AtSPSA1; young and mature, isozyme AtSPSC is one of the major isoforms expressed in leaves. Determination of sucrose efflux from source leaves. AtSPSC expression is about 75% lower than that of AtSPSA1
-
5 resp. 35% of total leaf enzyme located in bundle sheath cells
high expression level of SPSB, leaves of plants inoculated with Sinorhizobium meliloti
-
-
638626, 638634, 638636, 638637, 638638, 638639, 638640, 638641, 638643, 638645, 638646, 638647, 638649, 638651, 638652, 638657, 689554
-
significant increases in SPS in the initial phase of dehydration. The next phase of dehydration is characterized by changes in metabolism coinciding with net hexose sugar phosphorylation. This phase is characterized by a further significant increase in sucrose accumulation, with increased rates of net sucrose accumulation and maximum rates of SPS activity measured under both saturating and limiting conditions. SPS protein is also increased
-
significant increases in SPS in the initial phase of dehydration. The next phase of dehydration is characterized by changes in metabolism coinciding with net hexose sugar phosphorylation. This phase is characterized by a further significant increase in sucrose accumulation, with increased rates of net sucrose accumulation and maximum rates of SPS activity measured under both saturating and limiting conditions. SPS protein is also increased
-
-
SPS activity increases during slow dehydration, being stimulated by 30% when net CO2 assimilation declines by 40%. SPS activity of stressed leaves kept 4 h in air containing 5% CO2 or 2 d after rewatering is slightly increased or unchanged, respectively. SPS activity of well-hydrated leaves is hardly affected by low CO2. Increased SPS activity is mimicked, in nonstressed leaves, by a rapid dehydration within 4 h and by abscisic acid fed through the transpiration stream. Increase in SPS activity could be linked to ABA-based signalling during a drought stress
-
although the level of SPS mRNA and protein is lower in embryos than in leaf, enzymatic activity is higher
high expression level of SPSA, nodules of plants inoculated with Sinorhizobium meliloti
expression only in the vascular system of the siliques, including the funiculi
additional information
AtSPSA1 is most abundant in all tissues tested; isozyme AtSPSB shows by far the lowest expression of all isozymes in all organs analysed; no expression in stigma
additional information
AtSPSA1 is most abundant in all tissues tested; isozyme AtSPSB shows by far the lowest expression of all isozymes in all organs analysed; no expression in stigma
additional information
AtSPSA1 is most abundant in all tissues tested; isozyme AtSPSB shows by far the lowest expression of all isozymes in all organs analysed; no expression in stigma
additional information
AtSPSA1 is most abundant in all tissues tested; isozyme AtSPSB shows by far the lowest expression of all isozymes in all organs analysed; no expression in stigma
additional information
-
differential pattern of starch degradation and sucrose synthesis during ripening in banana cultivars. SPS activity during postharvest ripening correlates with the differential sucrose metabolism pattern in three cultivars
additional information
tissue specificity and diurnal change in gene expression of the sucrose phosphate synthase gene family, overview. Isozyme comparison: gene SPS1 is preferentially expressed in source tissues, whereas genes SPS2, SPS6, and SPS8 are expressed equally in source and sink tissues, mRNA levels of SPS1, SPS8, and SPS11 are considerably higher in seeds than in shoots and roots; tissue specificity and diurnal change in gene expression of the sucrose phosphate synthase gene family, overview. Isozyme comparison: gene SPS1 is preferentially expressed in source tissues, whereas genes SPS2, SPS6, and SPS8 are expressed equally in source and sink tissues, mRNA levels of SPS1, SPS8, and SPS11 are considerably higher in seeds than in shoots and roots; tissue specificity and diurnal change in gene expression of the sucrose phosphate synthase gene family, overview. Isozyme comparison: gene SPS1 is preferentially expressed in source tissues, whereas genes SPS2, SPS6, and SPS8 are expressed equally in source and sink tissues, mRNA levels of SPS1, SPS8, and SPS11 are considerably higher in seeds than in shoots and roots; tissue specificity and diurnal change in gene expression of the sucrose phosphate synthase gene family, overview. Isozyme comparison: gene SPS1 is preferentially expressed in source tissues, whereas genes SPS2, SPS6, and SPS8 are expressed equally in source and sink tissues, mRNA levels of SPS1, SPS8, and SPS11 are considerably higher in seeds than in shoots and roots; tissue specificity and diurnal change in gene expression of the sucrose phosphate synthase gene family, overview. Isozyme comparison: gene SPS1 is preferentially expressed in source tissues, whereas genes SPS2, SPS6, and SPS8 are expressed equally in source and sink tissues, mRNA levels of SPS1, SPS8, and SPS11 are considerably higher in seeds than in shoots and roots
additional information
tissue specificity and diurnal change in gene expression of the sucrose phosphate synthase gene family, overview. Isozyme comparison: gene SPS1 is preferentially expressed in source tissues, whereas genes SPS2, SPS6, and SPS8 are expressed equally in source and sink tissues, mRNA levels of SPS1, SPS8, and SPS11 are considerably higher in seeds than in shoots and roots; tissue specificity and diurnal change in gene expression of the sucrose phosphate synthase gene family, overview. Isozyme comparison: gene SPS1 is preferentially expressed in source tissues, whereas genes SPS2, SPS6, and SPS8 are expressed equally in source and sink tissues, mRNA levels of SPS1, SPS8, and SPS11 are considerably higher in seeds than in shoots and roots; tissue specificity and diurnal change in gene expression of the sucrose phosphate synthase gene family, overview. Isozyme comparison: gene SPS1 is preferentially expressed in source tissues, whereas genes SPS2, SPS6, and SPS8 are expressed equally in source and sink tissues, mRNA levels of SPS1, SPS8, and SPS11 are considerably higher in seeds than in shoots and roots; tissue specificity and diurnal change in gene expression of the sucrose phosphate synthase gene family, overview. Isozyme comparison: gene SPS1 is preferentially expressed in source tissues, whereas genes SPS2, SPS6, and SPS8 are expressed equally in source and sink tissues, mRNA levels of SPS1, SPS8, and SPS11 are considerably higher in seeds than in shoots and roots; tissue specificity and diurnal change in gene expression of the sucrose phosphate synthase gene family, overview. Isozyme comparison: gene SPS1 is preferentially expressed in source tissues, whereas genes SPS2, SPS6, and SPS8 are expressed equally in source and sink tissues, mRNA levels of SPS1, SPS8, and SPS11 are considerably higher in seeds than in shoots and roots
additional information
tissue specificity and diurnal change in gene expression of the sucrose phosphate synthase gene family, overview. Isozyme comparison: gene SPS1 is preferentially expressed in source tissues, whereas genes SPS2, SPS6, and SPS8 are expressed equally in source and sink tissues, mRNA levels of SPS1, SPS8, and SPS11 are considerably higher in seeds than in shoots and roots; tissue specificity and diurnal change in gene expression of the sucrose phosphate synthase gene family, overview. Isozyme comparison: gene SPS1 is preferentially expressed in source tissues, whereas genes SPS2, SPS6, and SPS8 are expressed equally in source and sink tissues, mRNA levels of SPS1, SPS8, and SPS11 are considerably higher in seeds than in shoots and roots; tissue specificity and diurnal change in gene expression of the sucrose phosphate synthase gene family, overview. Isozyme comparison: gene SPS1 is preferentially expressed in source tissues, whereas genes SPS2, SPS6, and SPS8 are expressed equally in source and sink tissues, mRNA levels of SPS1, SPS8, and SPS11 are considerably higher in seeds than in shoots and roots; tissue specificity and diurnal change in gene expression of the sucrose phosphate synthase gene family, overview. Isozyme comparison: gene SPS1 is preferentially expressed in source tissues, whereas genes SPS2, SPS6, and SPS8 are expressed equally in source and sink tissues, mRNA levels of SPS1, SPS8, and SPS11 are considerably higher in seeds than in shoots and roots; tissue specificity and diurnal change in gene expression of the sucrose phosphate synthase gene family, overview. Isozyme comparison: gene SPS1 is preferentially expressed in source tissues, whereas genes SPS2, SPS6, and SPS8 are expressed equally in source and sink tissues, mRNA levels of SPS1, SPS8, and SPS11 are considerably higher in seeds than in shoots and roots
additional information
tissue specificity and diurnal change in gene expression of the sucrose phosphate synthase gene family, overview. Isozyme comparison: gene SPS1 is preferentially expressed in source tissues, whereas genes SPS2, SPS6, and SPS8 are expressed equally in source and sink tissues, mRNA levels of SPS1, SPS8, and SPS11 are considerably higher in seeds than in shoots and roots; tissue specificity and diurnal change in gene expression of the sucrose phosphate synthase gene family, overview. Isozyme comparison: gene SPS1 is preferentially expressed in source tissues, whereas genes SPS2, SPS6, and SPS8 are expressed equally in source and sink tissues, mRNA levels of SPS1, SPS8, and SPS11 are considerably higher in seeds than in shoots and roots; tissue specificity and diurnal change in gene expression of the sucrose phosphate synthase gene family, overview. Isozyme comparison: gene SPS1 is preferentially expressed in source tissues, whereas genes SPS2, SPS6, and SPS8 are expressed equally in source and sink tissues, mRNA levels of SPS1, SPS8, and SPS11 are considerably higher in seeds than in shoots and roots; tissue specificity and diurnal change in gene expression of the sucrose phosphate synthase gene family, overview. Isozyme comparison: gene SPS1 is preferentially expressed in source tissues, whereas genes SPS2, SPS6, and SPS8 are expressed equally in source and sink tissues, mRNA levels of SPS1, SPS8, and SPS11 are considerably higher in seeds than in shoots and roots; tissue specificity and diurnal change in gene expression of the sucrose phosphate synthase gene family, overview. Isozyme comparison: gene SPS1 is preferentially expressed in source tissues, whereas genes SPS2, SPS6, and SPS8 are expressed equally in source and sink tissues, mRNA levels of SPS1, SPS8, and SPS11 are considerably higher in seeds than in shoots and roots
additional information
tissue specificity and diurnal change in gene expression of the sucrose phosphate synthase gene family, overview. Isozyme comparison: gene SPS1 is preferentially expressed in source tissues, whereas genes SPS2, SPS6, and SPS8 are expressed equally in source and sink tissues, mRNA levels of SPS1, SPS8, and SPS11 are considerably higher in seeds than in shoots and roots; tissue specificity and diurnal change in gene expression of the sucrose phosphate synthase gene family, overview. Isozyme comparison: gene SPS1 is preferentially expressed in source tissues, whereas genes SPS2, SPS6, and SPS8 are expressed equally in source and sink tissues, mRNA levels of SPS1, SPS8, and SPS11 are considerably higher in seeds than in shoots and roots; tissue specificity and diurnal change in gene expression of the sucrose phosphate synthase gene family, overview. Isozyme comparison: gene SPS1 is preferentially expressed in source tissues, whereas genes SPS2, SPS6, and SPS8 are expressed equally in source and sink tissues, mRNA levels of SPS1, SPS8, and SPS11 are considerably higher in seeds than in shoots and roots; tissue specificity and diurnal change in gene expression of the sucrose phosphate synthase gene family, overview. Isozyme comparison: gene SPS1 is preferentially expressed in source tissues, whereas genes SPS2, SPS6, and SPS8 are expressed equally in source and sink tissues, mRNA levels of SPS1, SPS8, and SPS11 are considerably higher in seeds than in shoots and roots; tissue specificity and diurnal change in gene expression of the sucrose phosphate synthase gene family, overview. Isozyme comparison: gene SPS1 is preferentially expressed in source tissues, whereas genes SPS2, SPS6, and SPS8 are expressed equally in source and sink tissues, mRNA levels of SPS1, SPS8, and SPS11 are considerably higher in seeds than in shoots and roots
additional information
-
high sugar cultivars showed increased transcript expression and enzyme activity of SPS compared to low sugar cultivars at all developmental stages
additional information
tissue specific semi-quantitative RT-PCR enzyme expression analysis
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
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PDB
SCOP
CATH
ORGANISM
UNIPROT
Halothermothrix orenii (strain H 168 / OCM 544 / DSM 9562)
Halothermothrix orenii (strain H 168 / OCM 544 / DSM 9562)
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
116000
118500
120000
138000
480000
116000
-
SDS-PAGE, minor 88000 Da band detected, different molecular weight for the two forms of enzyme postulated
116000
-
SDS-PAGE, minor 88000 Da band detected, that is suggested to be a degradation product
118500
x * 118500, about, sequence calculation, x * 120000, SDS-PAGE
120000
x * 118500, about, sequence calculation, x * 120000, SDS-PAGE
138000
-
SDS-PAGE, full length polypeptide, several bands with lower molecular weight detected
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SUBUNITS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
monomer
tetramer
additional information
additional information
two forms of recombinant enzyme, form A of 100 kDa and form B of 120 kDa, in Escherichia coli. Form B might be enzymatically more active than form A
additional information
protein motifs in the secondary structure, overview
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Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
hanging-drop vapor diffusion technique at 25°C. Crystal structure of SPS and its complexes with the substrate D-fructose 6-phosphate and the product D-sucrose-6'-phosphate. SPS has two distinct Rossmann-fold domains with a large substrate binding cleft at the interdomain interface. Structures of two complexes show that both the substrate D-fructose 6-phosphate and the product D-fructose 6'-phosphate bind to the A-domain of SPS. Halothermothrix orenii may represent a valid model for the catalytic domain of plant SPSs and thus may provide useful insight into the reaction mechanism of the plant enzyme
spsA protein crystallized in the monocyclic space group C2, with unit-cell parameters a = 154.2, b = 47.9, c = 103.16°, using hanging-drop vapour-diffusion method. Crystals diffract X-rays to a resolution limit of 3.01 A
-
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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
5.5 - 9
-
15 min, approximately 80% of the activity can be maintained in the range
658354
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
25
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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gel filtration is carried out in presence of 20% ethylene glycol and 0.2 M KCl to stabilize activity; partial
-
in presence of 2-mercaptoethanol and a phenol absorbing agent under N2; partial
-
native enzyme partially from plant tissue by ammonium sulfate fractionation and dialysis
partial
partial, two forms of the enzyme identified, enzyme becomes extremely unstable during the course of purification
-
partial, two forms of the enzyme identified, SPS-I loses more activity during purification than SPS-II
-
recombinant His6-tagged enzyme from Escherichia coli by nickel affinity chromatography to homogeneity
recombinant isozyme from Saccharomyces cerevisiae strain CY1905 by ammonium sulfate fractionation and two different steps of anion exchange chromatography; recombinant isozyme from Saccharomyces cerevisiae strain CY1905 by ammonium sulfate fractionation and two different steps of anion exchange chromatography; recombinant isozyme from Saccharomyces cerevisiae strain CY1905 by ammonium sulfate fractionation and two different steps of anion exchange chromatography; recombinant isozyme from Saccharomyces cerevisiae strain CY1905 by ammonium sulfate fractionation and two different steps of anion exchange chromatography
recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) and His8-myc-His8 tagged EGFP-enzyme from insect Sf9 cells by anion exchange and nickel affinity chromatography, followed by gel filtration. Purification of the full-length enzyme without His-tag by ion exchange and hydrophobic interaction chromatography, and gel filtration
two forms of the enzyme identified
native enzyme partially from plant tissue by ammonium sulfate fractionation and dialysis
-
native enzyme partially from plant tissue by ammonium sulfate fractionation and dialysis
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
analysis of transient expression of SPS genes, functional significance of the various cis-acting regulatory elements present in banana SPS promoter in regulating SPS expression during ripening
-
expressed in tomato and Escherichia coli, both reveal active enzyme, reduced amount of starch in leaves of transformed tomato
-
expression in Escherichia coli. The Synechocystis sps gene is introduced into tobacco, rice and tomato under the control of constitutive promoters. The Synechocystis SPS protein is expressed at high level. However SPS activities and carbon partitioning in leaves from transgenic and wild-type plants are not significantly different. The purified enzymes have full catalytic activity. It is proposed that some other protein in plant cells binds to the Synechocystis SPS resulting in inhibition of the enzyme
-
expression in Populus alba x Populus grandidentata causing an altered phenotype compared to the wild-type trees with altered timing of bud flush and leaf senescence. Tree height and stem diameter are similar to the wild-type, but differences in the length of xylem fibres occur. Elevated concentrations of intracellular sucrose in both leaf and stem tissue of the transgenic trees are associated with a prolonged onset of senescence and an advancement in bud flush in the following spring, phenotypes, overview
-
gene AtSPS5b, cloned with forward primer SPSa3 and reverse primer SPSb3, transfection via Agrobacterium tumefaciens strain EHA105 into Arabidopsis thaliana seedligs, transient recombinant expression of YFP-tagged enzyme in Nicotiana tabacum leaves and coexpression of sucrose phosphate phosphatase, recombinant expression in Saccharomyces cerevisiae strain MaV203 in a two-hybrid system with sucrose phosphate phosphatase, SPP, EC 3.1.3.24, AtSPP-AtSPS fusion is trabsfectde via Agrobacterium tumefaciens strain EHA105
gene KC-SPS1, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic tree
gene SoSPS1, recombinant expression of N-terminaly His6-tagged wild-type enzyme and truncated mutant enzymes lacking up to 171 residues of the N-terminal region in Escherichia coli strain BL21(DE3), recombinant expression of only the full-length wild-type enzyme with a His8-myc-His8 tag, a TEV cleavage site, and EGFP fusion in Spodoptera frugiperda Sf9 cells, the latter via baculovirus transfection
gene sps, quantitative expression analysis by RT-PCR
gene sps, sequence comparisons, phylogenetic analysis, recombinant expression of His6-tagged enzyme in Escherichia coli
gene SPS-1 or SPSA, overexpression in Medicago sativa driven by the constitutive CaMV35S promoter via Agrobacterium tumefaciens strain GV3101 transfection. Overexpression and increased activity of the enzyme in alfalfa is accompanied by early flowering, increased plant growth and an increase in elemental N and protein content when grown under N2-fixing conditions
gene sps1 or spsa1, recombinant expression in Saccharomyces cerevisiae strain CY1905, promoter-reporter gene analyses and quantitative real-time reverse transcription-PCR studies, isozyme expression patterns; gene sps1 or spsa1, recombinant expression in Saccharomyces cerevisiae strain CY1905, promoter-reporter gene analyses and quantitative real-time reverse transcription-PCR studies, isozyme expression patterns; gene sps2 or spsa2, recombinant expression in Saccharomyces cerevisiae strain CY1905, promoter-reporter gene analyses and quantitative real-time reverse transcription-PCR studies, isozyme expression patterns; gene sps3 or spsb, recombinant expression in Saccharomyces cerevisiae strain CY1905, promoter-reporter gene analyses and quantitative real-time reverse transcription-PCR studies, isozyme expression patterns
gene SPS1, real-time quantitative RT-PCR analysis of SPS1 expression in wild-type and transgenic plants
-
gene SPS1, recombinant expression driven by the CaMV35S promoter in leaves of Nicotiana tabacum via Agrobacterium tumefaciens strain GV3101 transfection method, coexpression with soybean glutamine synthetase
gene SPS11, phylogenetic tree of the SPS gene family, quantitative expression analysis by real-time RT-PCR analysis; gene SPS1, phylogenetic tree of the SPS gene family, quantitative expression analysis by real-time RT-PCR analysis; gene SPS2, phylogenetic tree of the SPS gene family, quantitative expression analysis by real-time RT-PCR analysis; gene SPS6, phylogenetic tree of the SPS gene family, quantitative expression analysis by real-time RT-PCR analysis; gene SPS8, phylogenetic tree of the SPS gene family, quantitative expression analysis by real-time RT-PCR analysis
genetic structure of SPSII genes, sequence comparisons and genetic mapping, phylogenetic tree, expression analysis of the SPSII family, overview
-
Nicotiana tabacum cv. Xanthi plants are transformed with an arabidopsis SPS gene under the regulation of the ubiquitously expressed tandem repeat of the 35S cauliflower mosaic virus promoter. All transformed plants have significantly increased stem height, which is ascribed to internode elongation, and greater stem diameters, longer fibers and increased total dry biomass relative to the control plants. Difference in the chemical composition of either the storage or structural carbohydrates of the wild-type and SPS transgenic lines are only minor
-
overexpression of maize sucrose-phosphate synthase gene in Nicotiana tabacum increases the sucrose synthesis and carbon assimilation, particularly in older leaves, accelerates the whole plant development and increases the abundance of flowers without substantial changes in the overall shoot biomass
-
transgenic cotton over-producing spinach sucrose phosphate synthase shows enhanced leaf sucrose synthesis and improved fiber quality under controlled environmental conditions
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
ethylene treatment differentially stimulates SPS gene expression, a reverse GCCbox, ACCGCCG, ethylene responsive element is located within the SPS promoters of the three cultivars
-
no significant correlation exists between the relative expression of sps and protein 14-3-3
SPS activity is positively correlated with sucrose and negatively correlated with hexose sugars
-
sucrose induces the enzyme, high sugar cultivars show increased transcript expression and enzyme activity of SPS compared to low sugar cultivars at all developmental stages. SPS activity is positively correlated with sucrose and negatively correlated with hexose sugars
-
no significant correlation exists between the relative expression of sps and protein 14-3-3
-
no significant correlation exists between the relative expression of sps and protein 14-3-3
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
additional information
construction of multiple knockout mutants of the four Arabidopsis sucrose phosphate synthase isozymes SPSA1, SPSA2, SPSB, and SPSC (T-DNA insertion mutants spsa1 (SALK 119162), spsa2 (SALK 064922), spsb (GABI 368F01) and spsc (SAIL 31 H05) and mutants spsa1/spsa2, spsa1/spsb, spsa1/spsc, spsa2/spsb, spsa2/spsc, spsb/spsc, spsa1/spsb/spsc, spsa1/spsa2/spsb, spsa1/spsa2/spsc, spsa2/spsb/spsc, and spsa1/spsa2/spsb/spsc). Despite their reduced SPS activity, mutants spsa1/spsa2, spsa1/spsb, spsa2/spsb, spsa2/spsc, spsb/spsc, spsa1/spsa2/spsb and spsa2/spsb/spsc display wild-type vegetative and reproductive morphology, and show wild-type photosynthetic capacity and respiration, phenotypes overview. Growth of rosettes, flowers and siliques of the spsa1/spsc and spsa1/spsa2/spsc mutants is reduced compared with wild-type plants. The plants display a high dark respiration phenotype. Mutant spsa1/spsb/spsc and spsa1/spsa2/spsb/spsc seeds poorly germinate and produce aberrant and sterile plants. Leaves of all viable sps mutants, except spsa1/spsc and spsa1/spsa2/spsc, accumulate wild-type levels of nonstructural carbohydrates. Mutant spsa1/spsc leaves possess high levels of metabolic intermediates and activities of enzymes of the glycolytic and tricarboxylic acid cycle pathways, and accumulate high levels of metabolic intermediates of the nocturnal starch-to-sucrose conversion process, even under continuous light conditions
additional information
-
enzyme downregulation by gene SPS1 antisense expression in transgenic plants using the CMV 35S promoter and Agrobacterium tumefaciens LBA4404 transfection. The transgenic plants show a phenotype with reduces sucrose phosphate synthase activity, fruit size and sucrose concentration, affected muskmelon leaf subcellular structure in SPS1 antisense muskmelon, overview
additional information
-
overexpression of the enzyme from Zea mays in Medicago sativa, gene SPS-1 or SPSA expression is driven by the constitutive CaMV35S promoter. Overexpression and increased activity of the enzyme in alfalfa is accompanied by early flowering, increased plant growth and an increase in elemental N and protein content when grown under N2-fixing conditions, phenotype, overview
additional information
overexpression of the enzyme from Zea mays in Medicago sativa, gene SPS-1 or SPSA expression is driven by the constitutive CaMV35S promoter. Overexpression and increased activity of the enzyme in alfalfa is accompanied by early flowering, increased plant growth and an increase in elemental N and protein content when grown under N2-fixing conditions, phenotype, overview
additional information
-
expression of the SPS gene from Arabidosis thaliana in Populus alba x Populus grandidentata causes an altered phenotype compared to the wild-type trees with altered timing of bud flush and leaf senescence. Tree height and stem diameter are similar to the wild-type, but differences in the length of xylem fibres occur. Elevated concentrations of intracellular sucrose in both leaf and stem tissue of the transgenic trees are associated with a prolonged onset of senescence and an advancement in bud flush in the following spring, phenotypes, overview
additional information
construction of N-terminally truncated enzymeslacking up to 171 amino acid residues in the 20 kDa N-terminal region, the mutants cannot be activated
additional information
production of transgenic tobacco plants with a cytosolic soybean glutamine synthetase (GS1) gene and a sucrose phosphate synthase (SPS) gene from maize, both driven by the CaMV35S promoter. The SPS enzyme activity in the SPS and SPS/GS1 co-transformants are the same under both nitrogen regimens, low and high nitrogen. The GS1/SPS transformants show a phenotype similar to the SPS transformants, phenotype overview
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
agriculture
sucrose phosphate synthase gene SPS is a suitable endogenous reference gene for genetically modified rice detection, method development and validation of the SPS gene as an endogenous reference gene and its optimized qualitative and quantitative PCR systems, overview
analysis
-
sucrose-phosphate synthase is a biochemical marker of high sucrose accumulation in sugarcane
diagnostics
sucrose phosphate synthase gene SPS is a suitable endogenous reference gene for genetically modified rice detection, method development and validation of the SPS gene as an endogenous reference gene and its optimized qualitative and quantitative PCR systems, overview
additional information
-
expression of the Arabidosis thaliana SPS gene in Populus alba x Populus grandidentata as model system for tree biology with substantial industrial relevance in the context of short rotation forestry and a target bioenergy crop
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LINKS TO OTHER DATABASES (specific for EC-Number 2.4.1.14)
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