Information on EC 2.4.1.14 - sucrose-phosphate synthase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY
2.4.1.14
-
RECOMMENDED NAME
GeneOntology No.
sucrose-phosphate synthase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
UDP-glucose + D-fructose 6-phosphate = UDP + sucrose 6F-phosphate
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
hexosyl group transfer
-
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
Metabolic pathways
-
Starch and sucrose metabolism
-
sucrose biosynthesis I (from photosynthesis)
-
sucrose biosynthesis II
-
sucrose biosynthesis III
-
SYSTEMATIC NAME
IUBMB Comments
UDP-glucose:D-fructose-6-phosphate 2-alpha-D-glucosyltransferase
Requires Mg2+ or Mn2+ for maximal activity [2]. The enzyme from Synechocystis sp. strain PCC 6803 is not specific for UDP-glucose as it can use ADP-glucose and, to a lesser extent, GDP-glucose as substrates [2]. The enzyme from rice leaves is activated by glucose 6-phosphate but that from cyanobacterial species is not [2]. While the reaction catalysed by this enzyme is reversible, the enzyme usually works in concert with EC 3.1.3.24, sucrose-phosphate phosphatase, to form sucrose, making the above reaction essentially irreversible [3]. The F in sucrose 6F-phosphate is used to indicate that the fructose residue of sucrose carries the substituent.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SPS
-
-
-
-
SPS
-
-
SPS
A2WYE9
-
SPS
B7F7B9, Q0JGK4, Q53JI9, Q67WN8, Q6ZHZ1
-
SPS
Oryza sativa Nipponbare
B7F7B9, Q0JGK4, Q53JI9, Q67WN8, Q6ZHZ1
-
-
SPS
-
-
SPS1
-
-
SPS1
Q0JGK4
-
SPS1
Oryza sativa Nipponbare
Q0JGK4
-
-
SPS11
Q53JI9
-
SPS11
Oryza sativa Nipponbare
Q53JI9
-
-
Sps2
B7F7B9
-
Sps2
Oryza sativa Nipponbare
B7F7B9
-
-
SPS6
Q67WN8
-
SPS6
Oryza sativa Nipponbare
Q67WN8
-
-
SPS8
Q6ZHZ1
-
SPS8
Oryza sativa Nipponbare
Q6ZHZ1
-
-
sucrose 6-phosphate synthase
-
-
-
-
sucrose phosphate synthase
-
-
sucrose phosphate synthase
-
-
sucrose phosphate synthase
-
-
sucrose phosphate synthase
-
-
sucrose phosphate synthase
-
-
sucrose phosphate synthase
-
-
sucrose phosphate synthase
-
-
sucrose phosphate synthase
A2WYE9
-
sucrose phosphate synthase
-
-
sucrose phosphate synthase
-
-
sucrose phosphate synthetase
-
-
-
-
sucrose phosphate-uridine diphosphate glucosyltransferase
-
-
-
-
sucrose-P synthase
-
-
sucrosephosphate-UDP glucosyltransferase
-
-
-
-
UDP-glucose-fructose-phosphate glucosyltransferase
-
-
-
-
UDP-glucose:D-fructose-6-phosphate 2-alpha-D-glucosyltransferase
-
-
-
-
uridine diphosphoglucose-fructose phosphate glucosyltransferase
-
-
-
-
additional information
-
two forms of enzyme identified, SPS-I and SPS-II differ in substrate specificity
additional information
Anabaena sp. 7119
-
two forms of enzyme identified, SPS-I and SPS-II differ in substrate specificity
-
additional information
-
two forms of enzyme identified, that differ in regulatory properties and stability
additional information
-
three dfferent isoforms of SPS belonging to two dfferent SPS gene families, SPSA and SPSB in Medicago sativa
additional information
-
SPS-I not affected by phosphate or glucose-6-phosphate; two forms of enzyme identified, that differ in regulatory properties
additional information
-
SPS-I shows high sensitivity towards phosphate, whereas SPS-II is only little sensitive, SPS-II is present in non-photosynthetic cells
additional information
-
two forms of enzyme identified, that differ in regulatory properties
additional information
-
two forms of enzyme identified, that differ in regulatory properties and stability
CAS REGISTRY NUMBER
COMMENTARY
9030-06-2
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
strain 7119
-
-
Manually annotated by BRENDA team
Anabaena sp. 7119
strain 7119
-
-
Manually annotated by BRENDA team
cv. Uladovskaya odnosemyannaya 35
-
-
Manually annotated by BRENDA team
sugar beet
-
-
Manually annotated by BRENDA team
soybean
-
-
Manually annotated by BRENDA team
barley
-
-
Manually annotated by BRENDA team
sweet potato, decreasing activity during root development
-
-
Manually annotated by BRENDA team
Tainong 57
-
-
Manually annotated by BRENDA team
cv. Mesa, plants inoculated with Sinorhizobium meliloti, three different isoforms of SPS belonging to two dfferent SPS gene families, SPSA and SPSB, in Medicago sativa
Uniprot
Manually annotated by BRENDA team
subgroup Cavendish, cv Giant governor
-
-
Manually annotated by BRENDA team
Oncidium Goldiana
-
-
-
Manually annotated by BRENDA team
gene SPS
UniProt
Manually annotated by BRENDA team
rice
-
-
Manually annotated by BRENDA team
SPS11; subsp. japonica
UniProt
Manually annotated by BRENDA team
SPS1; subsp. japonica
UniProt
Manually annotated by BRENDA team
SPS2; subsp. japonica
UniProt
Manually annotated by BRENDA team
SPS6; subsp. japonica
UniProt
Manually annotated by BRENDA team
SPS8; subsp. japonica
UniProt
Manually annotated by BRENDA team
Oryza sativa Nipponbare
SPS11; subsp. japonica
UniProt
Manually annotated by BRENDA team
Oryza sativa Nipponbare
SPS1; subsp. japonica
UniProt
Manually annotated by BRENDA team
Oryza sativa Nipponbare
SPS2; subsp. japonica
UniProt
Manually annotated by BRENDA team
Oryza sativa Nipponbare
SPS6; subsp. japonica
UniProt
Manually annotated by BRENDA team
Oryza sativa Nipponbare
SPS8; subsp. japonica
UniProt
Manually annotated by BRENDA team
kidney bean
-
-
Manually annotated by BRENDA team
L.cv. Montcalm
-
-
Manually annotated by BRENDA team
high sucrose accumulating cultivars CoS96268 and CoS95255, and late maturing, low sucrose accumulating cultivars CoS97264, CoSe92423
-
-
Manually annotated by BRENDA team
several cultivars., e.g. ROC20 and RB72-454
-
-
Manually annotated by BRENDA team
Sporobolus stapfianus Gandoger
Gandoger
-
-
Manually annotated by BRENDA team
Synechococcus marinus
-
-
-
Manually annotated by BRENDA team
PCC 6803
-
-
Manually annotated by BRENDA team
ladino clover
-
-
Manually annotated by BRENDA team
maize
-
-
Manually annotated by BRENDA team
cultered mesophyll cells
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
evolution
-
a comparison of the sequences of the wheat SPSII orthologues present in the diploid progenitors Triticum monococcum, Triticum urartu, Triticum speltoides, Aegilops tauschii, and Triticum speltoides, as well as in the more distantly related species Hordeum vulgare, Oryza sativa, Sorghum and purple false brome, Brachypodium distachyon, demonstrates that intronic sequence is less well conserved than exonic. Comparative sequence and phylogenetic analysis of SPSII gene shows that false purple brome is more similar to Triticeae than to Oryza sativa
metabolism
-, Q9AXK3
SPS catalyzes the first step in the synthesis of sucrose in photosynthetic tissue
metabolism
-
sucrose phosphate synthase, together with the soluble acid invertase, are the key enzymes in regulating sucrose accumulation in sugarcane stalk, overview
metabolism
-
sucrose phosphate synthase is an important component of the plant sucrose biosynthesis pathway
metabolism
-
sucrose phosphate synthase and sucrose synthase, EC 2.4.1.13, are key enzymes in the synthesis and breakdown of sucrose in sugarcane
physiological function
-
SPS plays a crucial role in carbohydrate metabolism by regulating the partitioning of carbon between starch production and carbohydrate accumulation in many physiological and developmental processes, including responses to water stress, diurnal carbohydrate allocation within plants, and fruit and flower development
physiological function
-
molecular mechanism of transcriptional regulation of banana sucrose phosphate synthase gene during fruit ripening by functions of various cis-acting regulatory elements, overview. Presence of specific trans-acting factors which showed specific interactions with ethylene, auxin, low temperature and light responsive elements in regulating SPS transcription
metabolism
G1UJV3, -
the sucrose phosphate synthase reaction is the key enzymatic step in sucrose synthesis in plants. Sucrose phosphate phosphatase and sucrose phosphate synthase catalyze sequential reactions in sucrose synthesis in green plant cells, the interaction between decreased sucrose phosphate phosphatase activity and sucrose phosphate synthase activity may alter sucrose synthesis during cold acclimation in Klebsormidium flaccidum, enzyme regulation, overview
additional information
-
gene expression analysis of sucrose biosynthesis genes during wheat plant ontogeny, overview
additional information
B7F7B9, Q0JGK4, Q53JI9, Q67WN8, Q6ZHZ1
expression of all the SPS genes, particularly that of SPS1 and SPS11, tends to be higher at night when the activation state of the SPS proteins is low, and the mRNA levels of SPS1 and SPS6 are negatively correlated with sucrose content. The temporal patterns of SPS gene expression and sugar content under continuous light conditions suggest the involvement of endogenous rhythm and/or sucrose sensing in the transcriptional regulation of SPS genes, overview; expression of all the SPS genes, particularly that of SPS1 and SPS11, tends to be higher at night when the activation state of the SPS proteins is low, and the mRNA levels of SPS1 and SPS6 are negatively correlated with sucrose content. The temporal patterns of SPS gene expression and sugar content under continuous light conditions suggest the involvement of endogenous rhythm and/or sucrose sensing in the transcriptional regulation of SPS genes, overview; expression of all the SPS genes, particularly that of SPS1 and SPS11, tends to be higher at night when the activation state of the SPS proteins is low, and the mRNA levels of SPS1 and SPS6 are negatively correlated with sucrose content. The temporal patterns of SPS gene expression and sugar content under continuous light conditions suggest the involvement of endogenous rhythm and/or sucrose sensing in the transcriptional regulation of SPS genes, overview; expression of all the SPS genes, particularly that of SPS1 and SPS11, tends to be higher at night when the activation state of the SPS proteins is low, and the mRNA levels of SPS1 and SPS6 are negatively correlated with sucrose content. The temporal patterns of SPS gene expression and sugar content under continuous light conditions suggest the involvement of endogenous rhythm and/or sucrose sensing in the transcriptional regulation of SPS genes, overview; expression of all the SPS genes, particularly that of SPS1 and SPS11, tends to be higher at night when the activation state of the SPS proteins is low, and the mRNA levels of SPS1 and SPS6 are negatively correlated with sucrose content. The temporal patterns of SPS gene expression and sugar content under continuous light conditions suggest the involvement of endogenous rhythm and/or sucrose sensing in the transcriptional regulation of SPS genes, overview
additional information
Oryza sativa Nipponbare
-
expression of all the SPS genes, particularly that of SPS1 and SPS11, tends to be higher at night when the activation state of the SPS proteins is low, and the mRNA levels of SPS1 and SPS6 are negatively correlated with sucrose content. The temporal patterns of SPS gene expression and sugar content under continuous light conditions suggest the involvement of endogenous rhythm and/or sucrose sensing in the transcriptional regulation of SPS genes, overview; expression of all the SPS genes, particularly that of SPS1 and SPS11, tends to be higher at night when the activation state of the SPS proteins is low, and the mRNA levels of SPS1 and SPS6 are negatively correlated with sucrose content. The temporal patterns of SPS gene expression and sugar content under continuous light conditions suggest the involvement of endogenous rhythm and/or sucrose sensing in the transcriptional regulation of SPS genes, overview; expression of all the SPS genes, particularly that of SPS1 and SPS11, tends to be higher at night when the activation state of the SPS proteins is low, and the mRNA levels of SPS1 and SPS6 are negatively correlated with sucrose content. The temporal patterns of SPS gene expression and sugar content under continuous light conditions suggest the involvement of endogenous rhythm and/or sucrose sensing in the transcriptional regulation of SPS genes, overview; expression of all the SPS genes, particularly that of SPS1 and SPS11, tends to be higher at night when the activation state of the SPS proteins is low, and the mRNA levels of SPS1 and SPS6 are negatively correlated with sucrose content. The temporal patterns of SPS gene expression and sugar content under continuous light conditions suggest the involvement of endogenous rhythm and/or sucrose sensing in the transcriptional regulation of SPS genes, overview; expression of all the SPS genes, particularly that of SPS1 and SPS11, tends to be higher at night when the activation state of the SPS proteins is low, and the mRNA levels of SPS1 and SPS6 are negatively correlated with sucrose content. The temporal patterns of SPS gene expression and sugar content under continuous light conditions suggest the involvement of endogenous rhythm and/or sucrose sensing in the transcriptional regulation of SPS genes, overview
-
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ADP-glucose + D-fructose 6-phosphate
ADP + sucrose 6-phosphate
show the reaction diagram
-
-
-
-
?
ADP-glucose + D-fructose 6-phosphate
ADP + sucrose 6-phosphate
show the reaction diagram
Anabaena sp., Anabaena sp. 7119
-
only SPS-I
-
?
GDP-glucose + D-fructose 6-phosphate
GDP + sucrose 6-phosphate
show the reaction diagram
-
-
-
-
?
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
-
-
r
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
-
-
r
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
-
-
-
?
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
-
-
r
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
-
-
r
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
-
-
r
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
-
-
-
?
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
-
-
r
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
-
-
r
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
-
-
r
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
-
-
r
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
-
-
r
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
-
-
-
?
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
-
-
-
?
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
-
-
-
?
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
-
-
r
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
-
-
-
?
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
B7F7B9, Q0JGK4, Q53JI9, Q67WN8, Q6ZHZ1
-
-
-
?
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
G1UJV3, -
-
-
-
?
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
-
equilibrium lies far on the product side
r
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
ordered bi-bi mechanism
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
ordered mechanism with UDP-glucose as first substrate bound and UDP as last product released
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
ordered mechanism, highly specific for its substrates
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
involved in regulation of carbon partitioning in leaves
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
involved in regulation of carbon partitioning in leaves
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
involved in regulation of carbon partitioning in leaves
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
supports synthesis of secondary wall cellulose by releasing UDP-glucose from sucrose
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
gene expression is minimal without illumination
-
-
?
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
high SPS activity in cold long days leading to hyper accumulation of sucrose appears to be among the features that permit Deschampsia antarctica to survive in the harsh Antarctic conditions
-
-
?
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
key enzyme of sucrose synthesis
-
-
?
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
overexpression of maize sucrose-phosphate synthase gene in Nicotiana tabacum increases the sucrose synthesis and carbon assimilation, particularly in older leaves, accelerates the whole plant development and increases the abundance of flowers without substantial changes in the overall shoot biomass
-
-
?
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
upregulation of the enzyme under elevated CO2 plus temperature
-
-
?
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
Oryza sativa Nipponbare
B7F7B9, Q0JGK4, Q53JI9, Q67WN8, Q6ZHZ1
-
-
-
?
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
Anabaena sp. 7119
-
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
Anabaena sp. 7119
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6F-phosphate
show the reaction diagram
-
-
-
-
?
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6F-phosphate
show the reaction diagram
-, Q9AXK3
isozyme show differences in allosteric regulation
-
-
?
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6F-phosphate
show the reaction diagram
-, Q9AXK3
optimal substrate concentrations are 10 mM for D-fructose 6-phosphate and 12 mM for UDP-glucose
-
-
?
GDP-glucose + D-fructose 6-phosphate
GDP + sucrose 6-phosphate
show the reaction diagram
Anabaena sp., Anabaena sp. 7119
-
only SPS-I
-
?
additional information
?
-
-
fructose or fructose 1-phosphate are not accepted as substrates
-
-
-
additional information
?
-
-
ADP-glucose can not replace UDP-glucose
-
-
-
additional information
?
-
-
development of homoeologue-specific assays, overview
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
-
-
-
?
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
-
-
-
?
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
-
-
-
?
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
B7F7B9, Q0JGK4, Q53JI9, Q67WN8, Q6ZHZ1
-
-
-
?
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
G1UJV3, -
-
-
-
?
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
involved in regulation of carbon partitioning in leaves
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
involved in regulation of carbon partitioning in leaves
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
involved in regulation of carbon partitioning in leaves
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
supports synthesis of secondary wall cellulose by releasing UDP-glucose from sucrose
-
-
-
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
gene expression is minimal without illumination
-
-
?
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
high SPS activity in cold long days leading to hyper accumulation of sucrose appears to be among the features that permit Deschampsia antarctica to survive in the harsh Antarctic conditions
-
-
?
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
key enzyme of sucrose synthesis
-
-
?
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
overexpression of maize sucrose-phosphate synthase gene in Nicotiana tabacum increases the sucrose synthesis and carbon assimilation, particularly in older leaves, accelerates the whole plant development and increases the abundance of flowers without substantial changes in the overall shoot biomass
-
-
?
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
-
upregulation of the enzyme under elevated CO2 plus temperature
-
-
?
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6F-phosphate
show the reaction diagram
-
-
-
-
?
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6F-phosphate
show the reaction diagram
-, Q9AXK3
isozyme show differences in allosteric regulation
-
-
?
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
Oryza sativa Nipponbare
B7F7B9, Q0JGK4, Q53JI9, Q67WN8, Q6ZHZ1
-
-
-
?
UDP-glucose + D-fructose 6-phosphate
UDP + sucrose 6-phosphate
show the reaction diagram
Anabaena sp. 7119
-
catalyzes the penultimate step of sucrose synthesis
-
-
-
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Ca2+
-
no effect
Mg2+
-
inhibitory
Mg2+
-
stimulates enzyme activity
Mg2+
-
reverses UDP inhibition
Mg2+
-
reverses inhibition with citrate, phosphate and nucloside triphosphates
Mg2+
-
no effect
Mg2+
-
stabilizing
Mg2+
-
stimulates enzyme activity
Mg2+
-
stimulates enzyme activity
Mg2+
-
stimulates enzyme activity
Mg2+
-
stimulates enzyme activity
Mg2+
-
stimulates enzyme activity
Mg2+
-, Q9AXK3
-
Mg2+
-
required
Mg2+
B7F7B9, Q0JGK4, Q53JI9, Q67WN8, Q6ZHZ1
required; required; required; required; required
Mn2+
-
inhibitory
Mn2+
-
stimulates enzyme activity
Mn2+
-
inhibitory
Mn2+
-
reverses UDP inhibition
Mn2+
-
stimulates enzyme activity
Mn2+
-
no effect
Mn2+
-
stimulates enzyme activity
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
5-azido-UDP-glucose
-
nearly complete inhibition at 1 mM
ADP
-
slight inhibition
ADP
-
no inhibition
ATP
-
reversible by addition of Mg2+
citrate
-
activity can by restored with Mg2+
CTP
-
reversible by addition of Mg2+
cycloheximide
-
reduces Vmax to one third of control when supplied to intact leaves, no effect on light activation
cycloheximide
-
no significant effect on Vmax, reduces extent of light activation
D-fructose 6-phosphate
-
inhibitory at high concentrations
delta-gluconolactone
-
-
delta-gluconolactone
-
-
diphosphate
-
slight inhibition
EDTA
-
complete inhibition, can be restored by addition of Mg2+ or Mn2+
fluoride
-
strong inhibition
fructose-1,6-bisphosphate
-
-
GTP
-
reversible by addition of Mg2+
ITP
-
reversible by addition of Mg2+
Maleate
-
-
-
NaCl
-
10% of activity remaining above 0.2 M
NaF
-
strong inhibition at 20 mM
okadaic acid
-
-
p-chloromercuribenzoate
-
activity can be restored by addition of DTT or 2-mercaptoethanol
p-chloromercuribenzoate
-
activity can be restored by addition of DTT or 2-mercaptoethanol
p-chloromercuribenzoate
-
-
phosphate
-
competitive with UDP-glucose
phosphate
-
inhibitory above 4 mM
phosphate
-
activity can by restored with Mg2+
phosphate
-
in absence of glucose-6-phosphate, suggested to be a metabolic regulator in vivo
phosphate
-
in absence of glucose-6-phosphate, no inhibition at pH 5.5, inhibitory at alkaline pH
phosphate
-
partial competitive inhibitor with respect to both substrates in presence of 5 mM glucose-6-phosphate, competes with glucose-6-phosphate for the same binding site
phosphate
-
suggested to be a metabolic regulator in vivo
phosphate
-
-
phosphate
-
-
phosphate
-
maximum inhibition with 1.5-2 mol phosphate per mol tetramer, maximum velocity is not affected
phosphate
-
competitive with UDP-glucose
phosphate
-
competitive with UDP-glucose
phosphate
-
E. coli expressed enzyme shows little sensitivity to phosphate inhibition, whereas tobacco expressed enzyme is highly phosphate sensitive
phosphate
-
only SPS-II is inhibited, SPS-I not affected
phosphate
-
little effect
phosphate
-
-
phosphate
-
conformational change suggested
phosphate
-
SPS-I shows high sensitivity towards phosphate, whereas SPS-II is only slightly sensitive
phosphate
-, Q9AXK3
86.3% inhibition of the nodule isozyme at 5 mM, 15.7% and 25.9% inhibition of the leaf enzyme at 2 mM and 5 mM, respectively. The inhibitory effect is increased in plants infected with an N-fixation deficient Sinorhizobium meliloti strain
phosphoenolpyruvate
-
slight inhibition
Sucrose
-
non-competitive with respect to fructose-6-phosphate
Sucrose
-
preincubation increases sensitivity to phosphate inhibition
sucrose-6-phosphate
-
-
Tris-HCl buffer
-
slight inhibition
UDP
-
competitive with UDP-glucose
UDP
-
competitive with UDP-glucose; reversible by addition of 5 mM Mn2+ or 10 mM Mg2+
UDP
-
competitive with UDP-glucose
UDP
-
competitive with UDP-glucose
additional information
-
no inhibition with sucrose phosphate
-
additional information
-
no inhibition with arsenate
-
additional information
-
preparation contains a protein kinase that can phosphorylate and therefore inactivate enzyme activity
-
additional information
-
no phosphate inhibition
-
additional information
-
mechanisms involved in short-term feedback inhibition of sucrose synthesis by sucrose are lacking in wheat
-
additional information
-
the Synechocystis sps gene is introduced into tobacco, rice and tomato under the control of constitutive promoters. The Synechocystis SPS protein is expressed at high level. However SPS activities and carbon partitioning in leaves from transgenic and wild-type plants are not significantly different. The purified enzymes have full catalytic activity. It is proposed that some other protein in plant cells binds to the Synechocystis SPS resulting in inhibition of the enzyme
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
1,5-Anhydroglucitol 6-phosphate
-
strong stimulation
1,5-Anhydroglucitol 6-phosphate
-
-
Benzyladenine
-
the phytohormone increases the specific activity of the enzyme practically during the entire growing period, except for the youngest leaves
D-fructose-1,6-diphosphate
-
-
D-fructose-1-phosphate
-
slight activation
D-glucose
-
-
D-glucose 6-phosphate
-, Q9AXK3
157-165% activation, the activation effect is reduced in plants infected with an N-fixation deficient Sinorhizobium meliloti strain
D-glucose-1-phosphate
-
slight activation
D-glucose-1-phosphate
-
-
D-Glucose-6-phosphate
-
the enzyme is activated in light, with an increased affinity for its substrates and the activator glucose-6-phosphate, reduces sensitivity to inhibition by phosphate, but no change in maximal catalytic activity
gibberellic acid
-
the phytohormone increases the specific activity of the enzyme practically during the entire growing period, except for the youngest leaves
glucosamine
-
-
glucose-6-phosphate
-
reduces Km of fructose-6-phosphate, suggested to be a metabolic regulator in vivo
glucose-6-phosphate
-
two enzyme species differ in their degree of activation
glucose-6-phosphate
-
-
glucose-6-phosphate
-
-
glucose-6-phosphate
-
only SPS-II is activated, SPS-I not affected
glucose-6-phosphate
-
little effect
glucose-6-phosphate
-
-
glucose-6-phosphate
-
SPS-I and SPS-II
IAA
-
the phytohormone increases specific activity of the enzyme practically during the entire growing period, except for the youngest leaves
light
-
-
-
light
-
-
-
light
-
active, dephosphorylated is formed in light period
-
light
-
induces covalent modification as well as de novo synthesis
-
Mannose
-
pretreatment lowers sensitivity to phosphate inhibition
Mg2+
-
stimulates enzyme activity
Mg2+
-
reverses UDP inhibition
Mg2+
-
stimulates enzyme activity
Mg2+
-
stimulates enzyme activity
Mg2+
-
stimulates enzyme activity
Mg2+
-
stimulates enzyme activity
Mg2+
-
required
Mg2+
-
stimulates enzyme activity
Mg2+
-
stimulates enzyme activity
Mn2+
-
stimulates enzyme activity
Mn2+
-
reverses UDP inhibition
Mn2+
-
stimulates enzyme activity
Mn2+
-
stimulates enzyme activity
phosphate
-
stimulating at low concentration particularly at high fructose-6-phosphate concentrations
Mn2+
-
required
additional information
-
no activation with glucose-6-phosphate
-
additional information
-
recombinant enzyme expressed in Escherichia coli is not activated by glucose 6-phosphate
-
additional information
-
although SPS activity does not display an endogenous rhythm of activity in continuous light, activation of SPS at the end of the dark period is observed. This activation is possibly controlled by covalent modification, because it is inhibited by okadaic acid while the SPS protein level does not significantly change. The highest SPS activity is observed after 21 days of cold acclimation under long day conditions. This increased activity is not related to an increase in SPS gene expression or protein content
-
additional information
-
increase of enzyme activity in leaves during earlier stages of fruit development until 12 days after anthesis, increase of enzyme activity in mesocarp tissue until day 8 after anthesis
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
2
-
ADP-glucose
-
pH 8.0, enzyme expressed in Nicotiana tabacum
2.7
-
ADP-glucose
-
pH 8.0, enzyme expressed in Escherichia coli
0.2
-
D-fructose 6-phosphate
-
pH 8.0, cosubstrate: UDP-glucose, enzyme expressed in Escherichia coli
0.3
-
D-fructose 6-phosphate
-
pH 8.0, cosubstrate: UDP-glucose, enzyme expressed in Nicotiana tabacum
0.7
-
D-fructose 6-phosphate
-
in presence of 5 mM glucose 6-phosphate
0.8
-
D-fructose 6-phosphate
-
-
0.8
-
D-fructose 6-phosphate
-
in presence of 5 mM glucose 6-phosphate
0.8
-
D-fructose 6-phosphate
-
SPS-I
1.1
-
D-fructose 6-phosphate
-
SPS-II
1.4
-
D-fructose 6-phosphate
-
in presence of 2.5 mM glucose 6-phosphate
2
-
D-fructose 6-phosphate
-
Km is not effected by addition of 20 mM glucose 6-phosphate or 15 mM phosphate
3
-
D-fructose 6-phosphate
-
-
3
-
D-fructose 6-phosphate
-
in presence of 0.5 mM glucose 6-phosphate
3.2
-
D-fructose 6-phosphate
-
in the absence of glucose 6-phosphate
3.5
-
D-fructose 6-phosphate
-
SPS-I
4.1
-
D-fructose 6-phosphate
-
in the absence of glucose 6-phosphate
4.1
-
D-fructose 6-phosphate
-
SPS-II
5.2
-
D-fructose 6-phosphate
-
after preincubation with mannose
5.3
-
D-fructose 6-phosphate
-
-
9.3
-
D-fructose 6-phosphate
-
after preincubation with sucrose
1.5
-
GDP-glucose
-
pH 8.0, enzyme expressed in Escherichia coli
2.4
-
GDP-glucose
-
pH 8.0, enzyme expressed in Nicotiana tabacum
1.3
-
UDP-glucose
-
-
1.3
-
UDP-glucose
-
SPS-I
1.8
-
UDP-glucose
-
pH 8.0, enzyme expressed in Nicotiana tabacum
1.9
-
UDP-glucose
-
in presence and absence of glucose 6-phosphate
2.2
-
UDP-glucose
-
pH 8.0, enzyme expressed in Escherichia coli
2.4
-
UDP-glucose
-
-
2.5
-
UDP-glucose
-
-
2.7
-
UDP-glucose
-
-
2.9
-
UDP-glucose
-
-
3
-
UDP-glucose
-
-
3.6
-
UDP-glucose
-
SPS-I
4.2
-
UDP-glucose
-
-
4.2
-
UDP-glucose
-
-
4.2
-
UDP-glucose
-
SPS-II
4.3
-
UDP-glucose
-
SPS-II
4.4
-
UDP-glucose
-
after preincubation with mannose
4.6
-
UDP-glucose
-
in presence of 20 mM glucose 6-phosphate
5.4
-
UDP-glucose
-
-
6.7
-
UDP-glucose
-
after preincubation with sucrose
7.1
-
UDP-glucose
-
-
7.4
-
UDP-glucose
-
-
20
-
UDP-glucose
-
-
31.3
-
UDP-glucose
-
-
77
-
UDP-glucose
-
in presence of 15 mM phosphate
Ki VALUE [mM]
Ki VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.8
-
fructose-1,6-bisphosphate
-
-
1.75
-
phosphate
-
-
11
-
phosphate
-
-
50
-
Sucrose
-
-
0.4
-
sucrose-6-phosphate
-
-
9.4
-
UDP-glucose
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.0025
-
-
Vmax in vivo
0.003
-
-
value about, mesocarp tissue, day of anthesis
0.0067
-
-
value about, mesocarp tissue, 2 days before anthesis
0.01
-
-
value about, mesocarp tissue, 20 days after anthesis
0.01167
-
-
value about, leaf, 20 days after anthesis
0.013
-
-
value about, mesocarp tissue, 16 days after anthesis; value about, mesocarp tissue, 4 days after anthesis
0.015
-
-
value about, leaf, 2 days before anthesis
0.0167
-
-
value about, leaf, 16 days after anthesis; value about, leaf, day of anthesis; value about, mesocarp tissue, 8 days and 12 days after anthesis
0.023
-
-
value about, leaf, 12 days after anthesis
0.025
-
-
value about, leaf, 4 days after anthesis
0.03
-
-
value about, leaf, 8 days after anthesis
0.0316
-
-, Q9AXK3
wild-type leaf isozyme from plants infected with an N-fixation deficient Sinorhizobium meliloti strain
0.0322
-
-, Q9AXK3
wild-type leaf isozyme from plants infected with a wild-type Sinorhizobium meliloti strain
0.04
-
-
purified SPS-I
0.0767
-
-, Q9AXK3
wild-type nodule isozyme from plants infected with an N-fixation deficient Sinorhizobium meliloti strain
0.0861
-
-, Q9AXK3
wild-type nodule isozyme from plants infected with a wild-type Sinorhizobium meliloti strain
0.25
-
-
purified SPS-II
0.68
-
-
purified enzyme
1.1
-
-
purified enzyme
2.9
-
-
purified enzyme
4.22
-
-
purified enzyme
25
-
-
purified enzyme
28
-
-
purified enzyme, specific activity in crude extract is extremely low compared to spinach
57
-
-
purified enzyme
79.5
-
-
purified enzyme
additional information
-
-
enzyme activity during fruit development in wild-type and transgenic antisense plants, overview
additional information
-
-
enzyme activity in banana pulp during fruit ripening, overview
additional information
-
-
sucrose concentrations in wild-type and transgenic plant leaves, overview
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
6
-
-
broad optimum
6.5
7.5
-
broad optimum
6.5
-
-
broad optimum
7.2
-
-, Q9AXK3
assay at
7.4
-
-
assay at
7.5
-
-
broad optimum
7.5
-
-
assay at
7.5
-
B7F7B9, Q0JGK4, Q53JI9, Q67WN8, Q6ZHZ1
assay at; assay at; assay at; assay at; assay at
8.5
-
-
recombinant enzyme expressed in Escherichia coli
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
4.5
9
-
very low activity at pH 4.5
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
25
-
-
assay at
37
-
-
assay at
TEMPERATURE RANGE
TEMPERATURE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
25
55
-
50% of activity at 55C
25
55
-
25C: about 70% of maximal activity, 55C: about 45% of maximal activity
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
-
maximal activity of SPS in illumination grown calli appears on the 14th day after the culture is transferred into a new medium. Very low activity in dark grown cells
Manually annotated by BRENDA team
-
although the level of SPS mRNA and protein is lower in embryos than in leaf, enzymatic activity is higher
Manually annotated by BRENDA team
-
the level of SPS transcript is 10fold lower in endosperm than in leaf but the level of SPS protein is comparable and the activity is 2fold higher
Manually annotated by BRENDA team
-
ethylene strongly stimulates SPS transcript accumulation, auxin and cold treatment only marginally increase the abundance of SPS mRNA level, while wounding negatively regulates SPS gene expression. SPS transcript level is distinctly increased by constant exposure to white light. Protein level, enzymatic activity of SPS and sucrose synthesis are substantially increased by ethylene and increased exposure to white light conditions as compared to other treatments
Manually annotated by BRENDA team
-
SPS gene expression during ripening, overview
Manually annotated by BRENDA team
-
activity of SPS is significantly higher in the upper internodes of high commercial cane sugar clones as compared with low commercial cane sugar clones in both populations, suggesting that this enzyme may have a key role in establishing metabolic and developmental processes necessary for high sugar accumulation during stem growth and maturation
Manually annotated by BRENDA team
-
enzyme activity in young intermodes of different cultivars, overview
Manually annotated by BRENDA team
-
SPS activity and transcript expression is higher in mature internodes compared with immature internodes in all the studied cultivars
Manually annotated by BRENDA team
-
highest activity in terminal stage of leaf development, 105 days
Manually annotated by BRENDA team
-
activity almost exclusively located in mesophyll
Manually annotated by BRENDA team
-
5 resp. 35% of total leaf enzyme located in bundle sheath cells
Manually annotated by BRENDA team
-
activity almost exclusively located in mesophyll
Manually annotated by BRENDA team
-
although the level of SPS mRNA and protein is lower in embryos than in leaf, enzymatic activity is higher
Manually annotated by BRENDA team
-
SPS activity increases during slow dehydration, being stimulated by 30% when net CO2 assimilation declines by 40%. SPS activity of stressed leaves kept 4 h in air containing 5% CO2 or 2 d after rewatering is slightly increased or unchanged, respectively. SPS activity of well-hydrated leaves is hardly affected by low CO2. Increased SPS activity is mimicked, in nonstressed leaves, by a rapid dehydration within 4 h and by abscisic acid fed through the transpiration stream. Increase in SPS activity could be linked to ABA-based signalling during a drought stress
Manually annotated by BRENDA team
-
significant increases in SPS in the initial phase of dehydration. The next phase of dehydration is characterized by changes in metabolism coinciding with net hexose sugar phosphorylation. This phase is characterized by a further significant increase in sucrose accumulation, with increased rates of net sucrose accumulation and maximum rates of SPS activity measured under both saturating and limiting conditions. SPS protein is also increased
Manually annotated by BRENDA team
-
SPS is very low in emerged etiolated leaves and increases in deetiolation
Manually annotated by BRENDA team
-, Q9AXK3
high expression level of SPSB, leaves of plants inoculated with Sinorhizobium meliloti
Manually annotated by BRENDA team
Oryza sativa Nipponbare
-
-
-
Manually annotated by BRENDA team
-
very low activity
Manually annotated by BRENDA team
B7F7B9, Q0JGK4, Q53JI9, Q67WN8, Q6ZHZ1
-
Manually annotated by BRENDA team
Oryza sativa Nipponbare
-
-
-
Manually annotated by BRENDA team
-, Q9AXK3
high expression level of SPSA, nodules of plants inoculated with Sinorhizobium meliloti
Manually annotated by BRENDA team
-
germinated
Manually annotated by BRENDA team
-
germinated
Manually annotated by BRENDA team
B7F7B9, Q0JGK4, Q53JI9, Q67WN8, Q6ZHZ1
-
Manually annotated by BRENDA team
Oryza sativa Nipponbare
-
-
-
Manually annotated by BRENDA team
B7F7B9, Q0JGK4, Q53JI9, Q67WN8, Q6ZHZ1
-
Manually annotated by BRENDA team
Oryza sativa Nipponbare
-
-
-
Manually annotated by BRENDA team
Sporobolus stapfianus Gandoger
-
significant increases in SPS in the initial phase of dehydration. The next phase of dehydration is characterized by changes in metabolism coinciding with net hexose sugar phosphorylation. This phase is characterized by a further significant increase in sucrose accumulation, with increased rates of net sucrose accumulation and maximum rates of SPS activity measured under both saturating and limiting conditions. SPS protein is also increased
-
Manually annotated by BRENDA team
additional information
-
no activity in roots
Manually annotated by BRENDA team
additional information
-
no activity in roots
Manually annotated by BRENDA team
additional information
-
differential pattern of starch degradation and sucrose synthesis during ripening in banana cultivars. SPS activity during postharvest ripening correlates with the differential sucrose metabolism pattern in three cultivars
Manually annotated by BRENDA team
additional information
-
high sugar cultivars showed increased transcript expression and enzyme activity of SPS compared to low sugar cultivars at all developmental stages
Manually annotated by BRENDA team
additional information
B7F7B9, Q0JGK4, Q53JI9, Q67WN8, Q6ZHZ1
tissue specificity and diurnal change in gene expression of the sucrose phosphate synthase gene family, overview. Isozyme comparison: gene SPS1 is preferentially expressed in source tissues, whereas genes SPS2, SPS6, and SPS8 are expressed equally in source and sink tissues, mRNA levels of SPS1, SPS8, and SPS11 are considerably higher in seeds than in shoots and roots; tissue specificity and diurnal change in gene expression of the sucrose phosphate synthase gene family, overview. Isozyme comparison: gene SPS1 is preferentially expressed in source tissues, whereas genes SPS2, SPS6, and SPS8 are expressed equally in source and sink tissues, mRNA levels of SPS1, SPS8, and SPS11 are considerably higher in seeds than in shoots and roots; tissue specificity and diurnal change in gene expression of the sucrose phosphate synthase gene family, overview. Isozyme comparison: gene SPS1 is preferentially expressed in source tissues, whereas genes SPS2, SPS6, and SPS8 are expressed equally in source and sink tissues, mRNA levels of SPS1, SPS8, and SPS11 are considerably higher in seeds than in shoots and roots; tissue specificity and diurnal change in gene expression of the sucrose phosphate synthase gene family, overview. Isozyme comparison: gene SPS1 is preferentially expressed in source tissues, whereas genes SPS2, SPS6, and SPS8 are expressed equally in source and sink tissues, mRNA levels of SPS1, SPS8, and SPS11 are considerably higher in seeds than in shoots and roots; tissue specificity and diurnal change in gene expression of the sucrose phosphate synthase gene family, overview. Isozyme comparison: gene SPS1 is preferentially expressed in source tissues, whereas genes SPS2, SPS6, and SPS8 are expressed equally in source and sink tissues, mRNA levels of SPS1, SPS8, and SPS11 are considerably higher in seeds than in shoots and roots
Manually annotated by BRENDA team
additional information
Oryza sativa Nipponbare
-
tissue specificity and diurnal change in gene expression of the sucrose phosphate synthase gene family, overview. Isozyme comparison: gene SPS1 is preferentially expressed in source tissues, whereas genes SPS2, SPS6, and SPS8 are expressed equally in source and sink tissues, mRNA levels of SPS1, SPS8, and SPS11 are considerably higher in seeds than in shoots and roots; tissue specificity and diurnal change in gene expression of the sucrose phosphate synthase gene family, overview. Isozyme comparison: gene SPS1 is preferentially expressed in source tissues, whereas genes SPS2, SPS6, and SPS8 are expressed equally in source and sink tissues, mRNA levels of SPS1, SPS8, and SPS11 are considerably higher in seeds than in shoots and roots; tissue specificity and diurnal change in gene expression of the sucrose phosphate synthase gene family, overview. Isozyme comparison: gene SPS1 is preferentially expressed in source tissues, whereas genes SPS2, SPS6, and SPS8 are expressed equally in source and sink tissues, mRNA levels of SPS1, SPS8, and SPS11 are considerably higher in seeds than in shoots and roots; tissue specificity and diurnal change in gene expression of the sucrose phosphate synthase gene family, overview. Isozyme comparison: gene SPS1 is preferentially expressed in source tissues, whereas genes SPS2, SPS6, and SPS8 are expressed equally in source and sink tissues, mRNA levels of SPS1, SPS8, and SPS11 are considerably higher in seeds than in shoots and roots; tissue specificity and diurnal change in gene expression of the sucrose phosphate synthase gene family, overview. Isozyme comparison: gene SPS1 is preferentially expressed in source tissues, whereas genes SPS2, SPS6, and SPS8 are expressed equally in source and sink tissues, mRNA levels of SPS1, SPS8, and SPS11 are considerably higher in seeds than in shoots and roots
-
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
PDB
SCOP
CATH
ORGANISM
Halothermothrix orenii (strain H 168 / OCM 544 / DSM 9562)
Halothermothrix orenii (strain H 168 / OCM 544 / DSM 9562)
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
46000
-
-
gel filtration
52000
-
-
SDS-PAGE, minor band, degradation product
116000
-
-
SDS-PAGE, minor 88000 Da band detected, different molecular weight for the two forms of enzyme postulated
116000
-
-
SDS-PAGE, minor 88000 Da band detected, that is suggested to be a degradation product
117000
-
-
calculated from DNA sequence
117600
-
-
calculated from DNA sequence
118500
-
-
calculated from DNA sequence
120000
-
-
SDS-PAGE
130000
140000
-
SDS-PAGE
138000
-
-
SDS-PAGE
138000
-
-
SDS-PAGE, full length polypeptide, several bands with lower molecular weight detected
240000
-
-
native PAGE
253000
-
-
sucrose density gradient centrifugation
270000
280000
-
gel filtration
270000
-
-
gel filtration after Mono Q affinity column
380000
390000
-
gel filtration, glycerol density gradient centrifugation
413000
-
-
gel filtration of crude leaf extracts
420000
-
-
gel filtration
443000
-
-
gel filtration
456000
-
-
gel filtration
460000
-
-
gel filtration
480000
-
-
gel filtration after ultrafiltration
480000
-
-
gel filtration
540000
-
-
native PAGE
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
-
x * 82000, SDS-PAGE
dimer
-
2 * 120000, sucrose density gradient centrifugation, SDS-PAGE
monomer
-
1 * 46000, gel filtration
monomer
Anabaena sp. 7119
-
1 * 46000, gel filtration
-
tetramer
-
4 * 120000
tetramer
-
4 * 116000, gel filtration, SDS-PAGE
tetramer
-
4 * 1300000-140000, native and SDS-PAGE
Crystallization/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
hanging-drop vapor diffusion technique at 25C. Crystal structure of SPS and its complexes with the substrate D-fructose 6-phosphate and the product D-sucrose-6'-phosphate. SPS has two distinct Rossmann-fold domains with a large substrate binding cleft at the interdomain interface. Structures of two complexes show that both the substrate D-fructose 6-phosphate and the product D-fructose 6'-phosphate bind to the A-domain of SPS. Halothermothrix orenii may represent a valid model for the catalytic domain of plant SPSs and thus may provide useful insight into the reaction mechanism of the plant enzyme
B8CZ51
spsA protein crystallized in the monocyclic space group C2, with unit-cell parameters a = 154.2, b = 47.9, c = 103.16, using hanging-drop vapour-diffusion method. Crystals diffract X-rays to a resolution limit of 3.01 A
-
pH STABILITY
pH STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5.5
9
-
15 min, approximately 80% of the activity can be maintained in the range
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
25
-
-
inactivates highly activated enzyme
37
-
-
stable for at least 20 min
GENERAL STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
SPS-I loses more activity during purification than SPS-II
-
2-mercaptoethanol and phenol absorbing agents stabilize activity
-
20% ethylene glycol and 0.2 M KCl stabilize activity
-
KF prevents inactivation at room temperature
-
sensitive to freezing and thawing
-
Mg2+ stabilizes activity
-
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-80C, purified enzyme stable for at least 4 months
-
0C, 4 weeks, 50% loss of activity
-
4C, stable for at least 1 week
-
liquid nitrogen, stable for at least 11 months
-
-10C, 2 months, 10% loss of activity
-
-20C, 1 month, no loss of activity, 1 year, 50-60% loss of activity
-
0-4C, stable in presence of 20% glycerol or 5 mM fructose-6-phosphate
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
partial, two forms of the enzyme identified, enzyme becomes extremely unstable during the course of purification
-
two forms of the enzyme identified
-
native isozymes partially by anion exchange chromatography
-, Q9AXK3
partial, two forms of the enzyme identified, SPS-I loses more activity during purification than SPS-II
-
partial, at least 3 additional proteins could not be removed
-
enzyme becomes extremely unstable during the course of purification
-
gel filtration is carried out in presence of 20% ethylene glycol and 0.2 M KCl to stabilize activity; partial
-
in presence of 2-mercaptoethanol and a phenol absorbing agent under N2; partial
-
two forms of the enzyme identified
-
transgenic SPS from tobacco and rice
-
complete separation from sucrose synthetase
-
two forms of the enzyme identified
-
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
antisence transformants with reduced activity were produced
-
expression in Populus alba x Populus grandidentata causing an altered phenotype compared to the wild-type trees with altered timing of bud flush and leaf senescence. Tree height and stem diameter are similar to the wild-type, but differences in the length of xylem fibres occur. Elevated concentrations of intracellular sucrose in both leaf and stem tissue of the transgenic trees are associated with a prolonged onset of senescence and an advancement in bud flush in the following spring, phenotypes, overview
-
Nicotiana tabacum cv. Xanthi plants are transformed with an arabidopsis SPS gene under the regulation of the ubiquitously expressed tandem repeat of the 35S cauliflower mosaic virus promoter. All transformed plants have significantly increased stem height, which is ascribed to internode elongation, and greater stem diameters, longer fibers and increased total dry biomass relative to the control plants. Difference in the chemical composition of either the storage or structural carbohydrates of the wild-type and SPS transgenic lines are only minor
-
gene SPS1, real-time quantitative RT-PCR analysis of SPS1 expression in wild-type and transgenic plants
-
expression in Escherichia coli
-
SPSA and SPSB, DNA and amino acid sequence determination and analysis
-, Q9AXK3
analysis of transient expression of SPS genes, functional significance of the various cis-acting regulatory elements present in banana SPS promoter in regulating SPS expression during ripening
-
gene SPS, cloning of SPS promoter region
-
gene SPS, qualitative and quantitative realtime PCR analysis, overview
A2WYE9
gene SPS11, phylogenetic tree of the SPS gene family, quantitative expression analysis by real-time RT-PCR analysis; gene SPS1, phylogenetic tree of the SPS gene family, quantitative expression analysis by real-time RT-PCR analysis; gene SPS2, phylogenetic tree of the SPS gene family, quantitative expression analysis by real-time RT-PCR analysis; gene SPS6, phylogenetic tree of the SPS gene family, quantitative expression analysis by real-time RT-PCR analysis; gene SPS8, phylogenetic tree of the SPS gene family, quantitative expression analysis by real-time RT-PCR analysis
B7F7B9, Q0JGK4, Q53JI9, Q67WN8, Q6ZHZ1
gene SPSII, semiquantitative PCR expression analysis
-
expression in Escherichia coli and tobacco, active enzyme
-
expression of a 26000 Da fragment in Escherichia coli
-
transgenic cotton over-producing spinach sucrose phosphate synthase shows enhanced leaf sucrose synthesis and improved fiber quality under controlled environmental conditions
P31928
expression in Escherichia coli. The Synechocystis sps gene is introduced into tobacco, rice and tomato under the control of constitutive promoters. The Synechocystis SPS protein is expressed at high level. However SPS activities and carbon partitioning in leaves from transgenic and wild-type plants are not significantly different. The purified enzymes have full catalytic activity. It is proposed that some other protein in plant cells binds to the Synechocystis SPS resulting in inhibition of the enzyme
-
genetic structure of SPSII genes, sequence comparisons and genetic mapping, phylogenetic tree, expression analysis of the SPSII family, overview
-
expressed in tomato
-
expressed in tomato and Escherichia coli, both reveal active enzyme, reduced amount of starch in leaves of transformed tomato
-
overexpression of maize sucrose-phosphate synthase gene in Nicotiana tabacum increases the sucrose synthesis and carbon assimilation, particularly in older leaves, accelerates the whole plant development and increases the abundance of flowers without substantial changes in the overall shoot biomass
-
EXPRESSION
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
ethylene treatment differentially stimulates SPS gene expression, a reverse GCCbox, ACCGCCG, ethylene responsive element is located within the SPS promoters of the three cultivars
-
SPS activity is positively correlated with sucrose and negatively correlated with hexose sugars
-
sucrose induces the enzyme, high sugar cultivars show increased transcript expression and enzyme activity of SPS compared to low sugar cultivars at all developmental stages. SPS activity is positively correlated with sucrose and negatively correlated with hexose sugars
-
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
additional information
-
enzyme downregulation by gene SPS1 antisense expression in transgenic plants using the CMV 35S promoter and Agrobacterium tumefaciens LBA4404 transfection. The transgenic plants show a phenotype with reduces sucrose phosphate synthase activity, fruit size and sucrose concentration, affected muskmelon leaf subcellular structure in SPS1 antisense muskmelon, overview
additional information
-
expression of the SPS gene from Arabidosis thaliana in Populus alba x Populus grandidentata causes an altered phenotype compared to the wild-type trees with altered timing of bud flush and leaf senescence. Tree height and stem diameter are similar to the wild-type, but differences in the length of xylem fibres occur. Elevated concentrations of intracellular sucrose in both leaf and stem tissue of the transgenic trees are associated with a prolonged onset of senescence and an advancement in bud flush in the following spring, phenotypes, overview
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
agriculture
A2WYE9
sucrose phosphate synthase gene SPS is a suitable endogenous reference gene for genetically modified rice detection, method development and validation of the SPS gene as an endogenous reference gene and its optimized qualitative and quantitative PCR systems, overview
diagnostics
A2WYE9
sucrose phosphate synthase gene SPS is a suitable endogenous reference gene for genetically modified rice detection, method development and validation of the SPS gene as an endogenous reference gene and its optimized qualitative and quantitative PCR systems, overview
analysis
-
sucrose-phosphate synthase is a biochemical marker of high sucrose accumulation in sugarcane
additional information
-
expression of the Arabidosis thaliana SPS gene in Populus alba x Populus grandidentata as model system for tree biology with substantial industrial relevance in the context of short rotation forestry and a target bioenergy crop