Information on EC 2.3.1.51 - 1-acylglycerol-3-phosphate O-acyltransferase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY
2.3.1.51
-
RECOMMENDED NAME
GeneOntology No.
1-acylglycerol-3-phosphate O-acyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate = CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Acyl group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of secondary metabolites
-
-
CDP-diacylglycerol biosynthesis
-
-
CDP-diacylglycerol biosynthesis I
-
-
CDP-diacylglycerol biosynthesis II
-
-
CDP-diacylglycerol biosynthesis III
-
-
Glycerolipid metabolism
-
-
Glycerophospholipid metabolism
-
-
Metabolic pathways
-
-
oleate biosynthesis III (cyanobacteria)
-
-
palmitoleate biosynthesis III (cyanobacteria)
-
-
phospholipid remodeling (phosphatidate, yeast)
-
-
stigma estolide biosynthesis
-
-
superpathway of phosphatidate biosynthesis (yeast)
-
-
triacylglycerol biosynthesis
-
-
SYSTEMATIC NAME
IUBMB Comments
acyl-CoA:1-acyl-sn-glycerol-3-phosphate 2-O-acyltransferase
Acyl-[acyl-carrier protein] can also act as an acyl donor. The animal enzyme is specific for the transfer of unsaturated fatty acyl groups.
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
1-acyl-sn-glycero-3-phosphate acyltransferase
-
-
-
-
1-acyl-sn-glycero-3-phosphate acyltransferase 1
Q99943
-
1-acyl-sn-glycero-3-phosphate acyltransferase 9
-
-
1-acyl-sn-glycerol 3-phosphate acyltransferase
-
-
-
-
1-acyl-sn-glycerol 3-phosphate acyltransferases
-
-
1-acyl-sn-glycerol-3-phosphate acyltransferase
Q643R3
-
1-acyl-sn-glycerol-3-phosphate acyltransferase
-
-
1-acyl-sn-glycerol-3-phosphate acyltransferase
P33333, Q08548
-
1-acylglycero-3-phosphate acyltransferase
-
-
-
-
1-acylglycerol 3-phosphate O-acyltransferase
P33333
-
1-acylglycerol-3-phosphate acyltransferase
-
-
1-acylglycerol-3-phosphate-O-acyltransferase
-
-
1-acylglycerol-3-phosphate-O-acyltransferase
O15120
-
1-acylglycerol-3-phosphate-O-acyltransferase 2
-
-
1-acylglycerol-3-phosphate-O-acyltransferase isoform10/glycerol-3-phosphate acyltransferase isoform 3
-
-
1-acylglycerol-3-phosphate-O-acyltransferase-1
O35083
-
1-acylglycerolphosphate acyltransferase
-
-
-
-
1-acylglycerolphosphate acyltransferase
Q643R3
-
1-acylglycerophosphate acyltransferase
-
-
-
-
1-Agpat2
O15120
-
ABHD5
Q9DBL9
-
acyl-coenzyme A-dependent lysophosphatidic acid acyltransferase
O22975
-
acyltransferase, 1-acylglycerol phosphate
-
-
-
-
AGPAT
O15120
-
AGPAT
Q643R3
-
AGPAT1
Q99943
-
AGPAT1
O35083
-
AGPAT10/GPAT3
-
-
AGPAT11
Q7L5N7
-
AGPAT2
-
-
AGPAT2
O15120
-
AGPAT3
-
-
AGPAT3
Q9NRZ7
-
AGPAT3
C4B4E7
-
AGPAT3
Mus musculus C57BL/6J
C4B4E7
-
-
AGPAT5
Q9NUQ2
-
AGPAT6/GPAT4
-
-
AGPAT9
-
-
At4g24160
O22975
gene name
CGFI-58
Q9DBL9
-
cgi-58 protein
-
-
CGI-58/ABHD5
-
-
coenzyme A-dependent lysophosphatidic acid
-
-
endoplasmic reticulum-located LPAT
Q8LG50
-
ER-located LPAT
Q8LG50
-
ICT1 protein
-
-
LPA acyltransferase
Q8GXU8, Q8L4Y2, Q8LG50, Q9LHN4, Q9SYC8
-
LPAAT
O22975
-
LPAAT
Q643R3
-
LPAAT3
-
-
LPAAT3
Mus musculus C57BL/6J
C4B4E7
-
-
LPAATbeta
O15120
-
LPAT
Q8GXU8, Q8L4Y2, Q8LG50, Q9LHN4, Q9SYC8
-
LPCAT2
Q7L5N7
-
lysophosphatidic acid acyl transferase
-
-
lysophosphatidic acid acyltransferase
-
-
lysophosphatidic acid acyltransferase
Q643R3
-
lysophosphatidic acid acyltransferase
Q99943
-
lysophosphatidic acid acyltransferase
-
-
lysophosphatidic acid acyltransferase 3
-
-
lysophosphatidic acid acyltransferase 3
C4B4E7
-
lysophosphatidic acid acyltransferase 3
Mus musculus C57BL/6J
C4B4E7
-
-
lysophosphatidic acid ayltransferase
-
-
lysophosphatidic acid ayltransferase
-
-
lysophosphatidic acid-acyltransferase
-
-
-
-
lysophosphatidic acidspecific acyltransferase
-
-
lysophosphatidyl acyltransferase
-
-
lysophosphatidyl acyltransferase
Q8GXU8, Q8L4Y2, Q8LG50, Q9LHN4, Q9SYC8
-
mAGPAT1
-
-
mAGPAT2
-
-
mAGPAT3
-
-
mAGPAT4
-
-
oleoyl-CoA lysophosphatidic acid acyltransferase
-
-
SMc00714 protein
-
-
YPR139c
Q06508
-
mAGPAT5
-
-
additional information
O22975
the enzyme belongs to the alpha/beta-hydrolase family
additional information
-
the enzyme belongs to the alpha/beta-hydrolase family
additional information
C4B4E7
the enzyme belongs to the 1-acylglycerol-3-phosphate O-acyltransferase, AGPAT, family
additional information
Mus musculus C57BL/6J
C4B4E7
the enzyme belongs to the 1-acylglycerol-3-phosphate O-acyltransferase, AGPAT, family
-
CAS REGISTRY NUMBER
COMMENTARY
51901-16-7
-
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
At4g24160; gene At4g24160
UniProt
Manually annotated by BRENDA team
LPAT1; gene LPAT1
SwissProt
Manually annotated by BRENDA team
LPAT2; gene LPAT2
SwissProt
Manually annotated by BRENDA team
LPAT3; gene LPAT3
SwissProt
Manually annotated by BRENDA team
LPAT4; gene LPAT4
SwissProt
Manually annotated by BRENDA team
LPAT5; gene LPAT5
SwissProt
Manually annotated by BRENDA team
Jersey cow
-
-
Manually annotated by BRENDA team
BAT1.13 and BAT1.5 genes encoding two microsomal LPAAT isozymes
UniProt
Manually annotated by BRENDA team
K12, strains: LCD45 and CE1061
-
-
Manually annotated by BRENDA team
XL1-Blue cells
-
-
Manually annotated by BRENDA team
Euglena sp.
-
-
-
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
AGPAT2; gene AGPAT2
SwissProt
Manually annotated by BRENDA team
gene AGPAT7; gene AGPAT7
SwissProt
Manually annotated by BRENDA team
gene AGPAT8
-
-
Manually annotated by BRENDA team
clone pLAT1
SwissProt
Manually annotated by BRENDA team
clone pLAT2
SwissProt
Manually annotated by BRENDA team
cvs. AC Emerson and Vimy
-
-
Manually annotated by BRENDA team
C57BL/6J mice
UniProt
Manually annotated by BRENDA team
gene AGPAT8
-
-
Manually annotated by BRENDA team
isozymes AGPAT1,2,3, and 5
-
-
Manually annotated by BRENDA team
Mus musculus C57BL/6J
C57BL/6J mice
UniProt
Manually annotated by BRENDA team
cv. Douce Provence; pea, cv. Kleine Rheinlaenderin
-
-
Manually annotated by BRENDA team
pea, cv. Kleine Rheinlaenderin
-
-
Manually annotated by BRENDA team
16 strains, genotypes listed
SwissProt
Manually annotated by BRENDA team
BY4741 and deletion mutant strain of ICT1 protein in BY4741-background
-
-
Manually annotated by BRENDA team
Saccharomyces cerevisiae OC-2
OC-2
-
-
Manually annotated by BRENDA team
gene plsC or SMc00714, strain RmP110
-
-
Manually annotated by BRENDA team
spinach, cv. Mona Lisa
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
malfunction
-
mutations in human CGI-58/ABHD5 cause Chanarin-Dorfman syndrome, characterized by excessive storage of triacylglycerol in tissues
metabolism
Q9XFW4
LPAAT is a key intermediate in the biosynthesis of membrane phospholipids in all tissues and storage lipids in developing seeds
metabolism
C4B4E7
the enzyme provides an an alternative important possibility to produce phosphatidylinositol in the testis
metabolism
Mus musculus C57BL/6J
-
the enzyme provides an an alternative important possibility to produce phosphatidylinositol in the testis
-
physiological function
O22975
LPAAT is a crucial enzyme controlling the metabolic flow of lysophosphatidic acid into the pool of phosphatidic acid, which plays a key role in many physiological aspects, such as cell signaling, cell polarity, and apoptotic signaling cascades in higher eukaryotes. Phosphatidic acid specifically regulates the genes involved in modulating cell shape and organization
physiological function
Q9XFW4
LPAAT plays an essential role in the synthesis of phosphatidic acid
physiological function
-
LPAAT3 regulates Golgi complex structure and function, overview
physiological function
Q9DBL9
expression of isoform CGI-58 in fibroblasts from humans with Chanarin-Dorfman increases the incorporation of fatty acids released from the lipolysis of stored triacylglycerols into phospholipids
physiological function
-
expression of isoform LPAATbeta is readily detected in 8 of 10 analyzed human osteosarcoma lines. Exogenous expression of LPAATbeta promotes osteosarcoma cell proliferation and migration, while silencing LPAATbeta expression inhibits these cellular characteristics.Exogenous expression of LPAATbeta effectively promotes tumor growth, while knockdown of LPAATbeta expression inhibits tumor growth in an orthotopic xenograft model of human osteosarcoma
physiological function
O35083
isoform AGPAT1 is involved in development of skeletal muscle. Small interference RNA-mediated knockdown of AGPAT1 expression prevents the induction of myogenin, and inhibits the expression of myosin heavy chain. This effect is rescued by transfection with AGPAT1 but not AGPAT2. The regulation of myogenesis by AGPAT1 is associated with alterations on actin cytoskeleton. AGPAT1 colocalizes AGPAT1 to areas of active actin polymerization. AGPAT1 overexpression is not associated with an increase in phosphatidic acid levels
physiological function
-
isoform LPAAT3 is induced during germ cell maturation. Differentiation of mouse GC-2spd(ts) spermatocytes into spermatides up-regulates isoform LPAAT3 mRNA, increases the amount of polyunsaturated phospholipids, and shifts the specificity for the incorporation of docosahexaenoic acid toward phosphatidylcholine and phosphatidylethanolamine. Stable knockdown of LPAAT3 in GC-2spd(ts) cells significantly decreases microsomal LPAAT3 activity, reduces levels of polyunsaturated phosphatidylethanolamine species, and impairs cell proliferation/survival during geneticin selection
physiological function
-
stable transfection of TM4 Sertoli cells with isoform LPAAT3-small hairpin RNA leads to decreases in arachidonoyl-, eicosapentaenoyl-, and docosapentaenoyl-containing phosphatidylcholine and linoleoyl-containing phosphatidylethanolamine, phosphatidylserine, and phosphatidylglycerol. Expression of murine LPAAT3 in Chinese hamster ovary K1 cells has essentially an opposite effect. The level of polyunsaturated phosphatidylcholine correlates with cellular levels of free docosapentaenoic acid and eicosapentaenoic acid in TM4 and Chinese hamster ovary K1 cells, respectively
physiological function
Q06508
strains deficient for the enzyme show abnormalities in lipid droplet morphology and contain more lipid droplets, while overexpression results in fewer lipid droplets. Mutant strains reveal a significant reduction in triacylglycerol content, while phospholipid composition remains unchanged. Both phopholipids and glycerolipids are qualitatively affected by the mutation
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1-oleoyl-sn-glycerol-3-phosphate + oleoyl-CoA
CoA + 1,2-oleoyl-sn-glycerol-3-phosphate
show the reaction diagram
-
-
-
-
?
1-oleoyl-sn-glycerol-3-phosphate + palmitoyl-CoA
CoA + 1-oleoyl-2-palmitoyl-sn-glycerol-3-phosphate
show the reaction diagram
-
-
-
-
?
acyl-ACP + 1-acyl-sn-glycerol 3-phosphate
ACP + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
-
negligible
-
?
acyl-ACP + 1-acyl-sn-glycerol 3-phosphate
ACP + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
-
catalyzes acylation of position 2 hydroxyl-group, acyl-donor: highly specific for palmitoyl-ACP preferred to oleoyl-ACP, acyl-acceptors are: 1-oleoyl-sn-glycerol 3-phosphate, which is the best and 1-palmitoyl-sn-glycerol 3-phosphate
-
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
-
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
-
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
-
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
Euglena sp.
-
-
-
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
Q99943
-
-
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
-
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
Q643R3
-
-
-
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
O15120
-
-
-
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
C4B4E7
-
-
-
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
i.e. phosphatidic acid
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
i.e. phosphatidic acid
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
i.e. phosphatidic acid
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
i.e. phosphatidic acid
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
i.e. phosphatidic acid
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
-
the enzyme is active towards acyl-CoAs of chain length C-8 to C-18
-
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
-
i.e. lysophosphatidic acid, highly specific for 1-acyl-sn-glycerol 3-phosphate, catalyzes acylation of position 2 hydroxyl-group
i.e. phosphatidic acid
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
-
i.e. lysophosphatidic acid, highly specific for 1-acyl-sn-glycerol 3-phosphate, catalyzes acylation of position 2 hydroxyl-group
i.e. phosphatidic acid
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
-
i.e. lysophosphatidic acid, highly specific for 1-acyl-sn-glycerol 3-phosphate, catalyzes acylation of position 2 hydroxyl-group
i.e. phosphatidic acid
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
-
i.e. lysophosphatidic acid, highly specific for 1-acyl-sn-glycerol 3-phosphate, catalyzes acylation of position 2 hydroxyl-group
i.e. phosphatidic acid
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
-
i.e. lysophosphatidic acid, highly specific for 1-acyl-sn-glycerol 3-phosphate, catalyzes acylation of position 2 hydroxyl-group
i.e. phosphatidic acid
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
-
CoA esters of unsaturated 16- and 18-carbon fatty acids are the best substrates, followed by those saturated 14-, 16-, and 18-carbon acids, and the unsaturated 20-carbon acids
-
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
-
C-14 to C-22
-
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
-
acyl-donors are saturated and unsaturated fatty acyl-CoAs
-
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
-
acyl-donors are saturated and unsaturated fatty acyl-CoAs
-
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
-
omega-3 and omega-6 polyunsaturated fatty acyl-CoAs
-
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
-
1 to 6 double bonds
i.e. phosphatidic acid
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
-
recombinant enzyme can catalyse ATP-independent acyl-CoA synthetic activity and CoA-dependent transacylation activity. Acyl-CoA synthetase is not involved in the process
-
r
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
-
with 1 to 5 double bonds
-
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
-
with 1 to 5 double bonds
-
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
-
with 1 to 5 double bonds
-
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
-
with 1 to 5 double bonds, unsaturated preferred
i.e. phosphatidic acid
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
-
unsaturated preferred
i.e. phosphatidic acid
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
-
monoenic and dienic acyl-CoA thioesters
i.e. phosphatidic acid
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
-
C-16, C-18, C-20 acyl-CoAs and C-22 acyl-CoA
i.e. phosphatidic acid
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
-
effect of substrate concentration, the enzyme utilizes saturated and unsaturated acyl-CoAs at comparable rates
i.e. phosphatidic acid
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
-
pronounced specificities and selectivities for unsaturated C18-CoA thioesters
i.e. phosphatidic acid
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
Q9DBL9
i.e. lysophosphatidic acid
i.e. phosphatidic acid
-
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
Q9XFW4
i.e. lysophosphatidic acid
i.e. phosphatidic acid
-
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
O22975
i.e. lysophosphatidic acid
i.e. phosphatidic acid
-
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
-
involved in phospholipid biosynthesis
-
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
-
involved in phospholipid biosynthesis
-
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
-
involved in phospholipid biosynthesis
-
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
-
involved in phospholipid biosynthesis
-
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
-
involved in phospholipid biosynthesis
-
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
-
involved in phospholipid biosynthesis
-
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
-
involved in phospholipid biosynthesis
-
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
-
pivotal enzyme controlling the metabolic flow of lysophosphatidic acid into different phosphatidic acids in diverse tissues, enzyme is essential in embryo development
-
-
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
-
reduction in AGPAT2 activity underlies the loss of adipose tissue in congenital generalized lipodystrophy
-
-
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
O15120
AGPAT2 catalyzes acylation of lysophosphatidic acid to phosphatidic acid, a precursor for both triacylglycerol and phospholipid synthesis, isozyme AGPAT2 plays a regulatory role in adipocyte differentiation
-
-
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
-
the enzyme catalyzes the second acylations in the glycerol-3-phosphate pathway
-
-
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
-
the expression of AGPATs is linked to skin barrier requirements
-
-
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
-
the enzyme catalyzes the transfer of the fatty acid from an acyl donor to the sn-2-position of lysophosphatidic acid
-
-
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
Q643R3
the enzyme catalyzes the transfer of the fatty acid from an acyl donor to the sn-2-position of lysophosphatidic acid
-
-
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
-
the preferred substrate of LPAAT is 18:2-CoA
-
-
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
Q8NF37
activity and expression pattern of AGPAT9 in lung and spleen implicates role in the biosynthesis of phospholipids and triglycerides in these tissues
-
-
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
P33333, Q08548
acyltranferase activity by Slc1p and Slc4p
-
-
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
P33333, Q08548
Slc1p and Slc4p both active as acyltransferases, involved in fatty acid exchange of mature glycerophospholipids
-
-
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
-
acylation of lysophosphatidic acid to phosphatidic acid, precursor for triacylglycerol and phospholipid synthesis
-
-
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
Q8NF37
cloning and characterization of isoform AGPAT9
-
-
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
P33333, Q08548
glycerophospholipid profile similar between Slc1p and Slc4p
-
-
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
-
substrate preference analyzed, assays performed with different acyl donors
-
-
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
Q9DBL9
i.e. lysophosphatidic acid, the recombinant CGI-58 shows preference for arachidonoyl-CoA and oleoyl-CoA, and slight preference for 1-oleoyl lysophosphatidic acid over 1-palmitoyl, 1-stearoyl, or 1-arachidonoyl lysophosphatidic acid
i.e. phosphatidic acid
-
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
C4B4E7
preferred donor substrate is arachidonoyl-CoA, no activity with oleoyl-CoA, strong activity with arachidonoyl-CoA and palmitoyl-lysophosphatidic acid, stearoyl-lysophosphatidic acid, oleoyl-lysophosphatidic acid, and octadecenyl-lysophosphatidic acid as acceptors
-
-
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
Mus musculus C57BL/6J
C4B4E7
preferred donor substrate is arachidonoyl-CoA, no activity with oleoyl-CoA, strong activity with arachidonoyl-CoA and palmitoyl-lysophosphatidic acid, stearoyl-lysophosphatidic acid, oleoyl-lysophosphatidic acid, and octadecenyl-lysophosphatidic acid as acceptors
-
-
?
acyl-CoA + 1-arachidonoyl-sn-glycerol 3-phosphate
CoA + 1-arachidonoyl-2-acyl-sn-glycerol 3-phosphate
show the reaction diagram
-
less efficient than 1-acyl-sn-glycerol 3-phosphate
-
?
acyl-CoA + 1-arachidonoyl-sn-glycerol 3-phosphate
CoA + 1-arachidonoyl-2-acyl-sn-glycerol 3-phosphate
show the reaction diagram
-
less efficient than 1-acyl-sn-glycerol 3-phosphate
-
?
acyl-CoA + 1-lauroyl-sn-glycerol 3-phosphate
CoA + 1-lauroyl-2-acyl-sn-glycerol 3-phosphate
show the reaction diagram
-
assayed using coconut cDNA encoding a 299-amino acid protein with enzyme activity expressed in canola seeds
-
?
acyl-CoA + 1-O-alkyl-sn-glycerol 3-phosphate
CoA + ?
show the reaction diagram
-
the activity is approximately half of that toward 1-acyl-sn-glycerol 3-phosphate
-
?
acyl-CoA + 1-oleoyl-sn-glycerol 3-phosphate
CoA + 2-acyl-1-oleoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
acyl-CoA + 1-oleoyl-sn-glycerol 3-phosphate
CoA + 2-acyl-1-oleoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
acyl-CoA + 1-oleoyl-sn-glycerol 3-phosphate
CoA + 2-acyl-1-oleoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
acyl-CoA + 1-oleoyl-sn-glycerol 3-phosphate
CoA + 2-acyl-1-oleoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
acyl-CoA + 1-oleoyl-sn-glycerol 3-phosphate
CoA + 2-acyl-1-oleoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
acyl-CoA + 1-oleoyl-sn-glycerol 3-phosphate
CoA + 2-acyl-1-oleoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
acyl-CoA + 1-oleoyl-sn-glycerol 3-phosphate
CoA + 2-acyl-1-oleoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
acyl-CoA + 1-oleoyl-sn-glycerol 3-phosphate
CoA + 2-acyl-1-oleoyl-sn-glycerol 3-phosphate
show the reaction diagram
Q59618
-
-
?
acyl-CoA + 1-oleoyl-sn-glycerol 3-phosphate
CoA + 2-acyl-1-oleoyl-sn-glycerol 3-phosphate
show the reaction diagram
Q59601
-
-
?
acyl-CoA + 1-oleoyl-sn-glycerol 3-phosphate
CoA + 2-acyl-1-oleoyl-sn-glycerol 3-phosphate
show the reaction diagram
Q99943
-
-
?
acyl-CoA + 1-oleoyl-sn-glycerol 3-phosphate
CoA + 2-acyl-1-oleoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
1-oleoyl-sn-glycerol 3-phosphate is the best acceptor
-
?
acyl-CoA + 1-palmitoyl-sn-glycerol 3-phosphate
CoA + 1-palmitoyl-2-acyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
r
acyl-CoA + 1-palmitoyl-sn-glycerol 3-phosphate
CoA + 1-palmitoyl-2-acyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
acyl-CoA + 1-palmitoyl-sn-glycerol 3-phosphate
CoA + 1-palmitoyl-2-acyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
acyl-CoA + 1-palmitoyl-sn-glycerol 3-phosphate
CoA + 1-palmitoyl-2-acyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
acyl-CoA + 1-palmitoyl-sn-glycerol 3-phosphate
CoA + 1-palmitoyl-2-acyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
acyl-CoA + 1-palmitoyl-sn-glycerol 3-phosphate
CoA + 1-palmitoyl-2-acyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
acyl-CoA + 1-palmitoyl-sn-glycerol 3-phosphate
CoA + 1-palmitoyl-2-acyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
acyl-CoA + 1-palmitoyl-sn-glycerol 3-phosphate
CoA + 1-palmitoyl-2-acyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
acyl-CoA + 1-palmitoyl-sn-glycerol 3-phosphate
CoA + 1-palmitoyl-2-acyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
-
acyl-CoA + 1-palmitoyl-sn-glycerol 3-phosphate
CoA + 1-palmitoyl-2-acyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
acyl-CoA + 1-palmitoyl-sn-glycerol 3-phosphate
CoA + 1-palmitoyl-2-acyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
acyl-CoA + 1-palmitoyl-sn-glycerol 3-phosphate
CoA + 1-palmitoyl-2-acyl-sn-glycerol 3-phosphate
show the reaction diagram
Euglena sp.
-
-
-
?
acyl-CoA + 1-stearoyl-sn-glycerol 3-phosphate
CoA + 1-stearoyl-2-acyl-sn-glycerol 3-phosphate
show the reaction diagram
-
less efficient than 1-acyl-sn-glycerol 3-phosphate
-
?
acyl-CoA + 1-stearoyl-sn-glycerol 3-phosphate
CoA + 1-stearoyl-2-acyl-sn-glycerol 3-phosphate
show the reaction diagram
-
less efficient than 1-acyl-sn-glycerol 3-phosphate
-
?
acyl-CoA + 2-acyl-sn-glycerol 3-phosphate
CoA + ?
show the reaction diagram
-
the enzyme does not acylate 2-acyl-sn-glycerol 3-phosphate
-
-
-
acyl-CoA + 2-acyl-sn-glycerol 3-phosphate
CoA + ?
show the reaction diagram
-
the enzyme does not acylate 2-acyl-sn-glycerol 3-phosphate
-
-
-
acyl-CoA + 2-acyl-sn-glycerol 3-phosphate
CoA + ?
show the reaction diagram
-
the enzyme does not acylate 2-acyl-sn-glycerol 3-phosphate
-
-
-
acyl-CoA + 2-acyl-sn-glycerol 3-phosphate
CoA + ?
show the reaction diagram
-
the rate of CoA-liberation is much lower than with 1-acyl-sn-glycerol 3-phosphate
-
?
acyl-CoA + linoleoyl-sn-glycerol 3-phosphate
CoA + ?
show the reaction diagram
-
-
-
?
acyl-CoA + linoleoyl-sn-glycerol 3-phosphate
CoA + ?
show the reaction diagram
-
-
-
?
acyl-CoA + linoleoyl-sn-glycerol 3-phosphate
CoA + ?
show the reaction diagram
-
-
-
?
acyl-CoA + linoleoyl-sn-glycerol 3-phosphate
CoA + ?
show the reaction diagram
-
-
-
?
acyl-CoA + linoleoyl-sn-glycerol 3-phosphate
CoA + ?
show the reaction diagram
-
-
-
?
acyl-CoA + linoleoyl-sn-glycerol 3-phosphate
CoA + ?
show the reaction diagram
-
-
-
?
acyl-CoA + linoleoyl-sn-glycerol 3-phosphate
CoA + ?
show the reaction diagram
-
-
-
-
acyl-CoA + linoleoyl-sn-glycerol 3-phosphate
CoA + ?
show the reaction diagram
-
-
-
?
acyl-CoA + linoleoyl-sn-glycerol 3-phosphate
CoA + ?
show the reaction diagram
-
-
-
?
acyl-CoA + linoleoyl-sn-glycerol 3-phosphate
CoA + ?
show the reaction diagram
Euglena sp.
-
-
-
?
acyl-CoA + myristoyl-sn-glycerol 3-phosphate
CoA + ?
show the reaction diagram
-
-
-
?
acyl-CoA + myristoyl-sn-glycerol 3-phosphate
CoA + ?
show the reaction diagram
-
-
-
?
acyl-CoA + myristoyl-sn-glycerol 3-phosphate
CoA + ?
show the reaction diagram
-
-
-
?
acyl-CoA + myristoyl-sn-glycerol 3-phosphate
CoA + ?
show the reaction diagram
-
-
-
?
acyl-CoA + myristoyl-sn-glycerol 3-phosphate
CoA + ?
show the reaction diagram
-
-
-
?
acyl-CoA + myristoyl-sn-glycerol 3-phosphate
CoA + ?
show the reaction diagram
-
-
-
?
acyl-CoA + myristoyl-sn-glycerol 3-phosphate
CoA + ?
show the reaction diagram
-
-
-
-
acyl-CoA + myristoyl-sn-glycerol 3-phosphate
CoA + ?
show the reaction diagram
-
-
-
?
acyl-CoA + myristoyl-sn-glycerol 3-phosphate
CoA + ?
show the reaction diagram
-
-
-
?
acyl-CoA + myristoyl-sn-glycerol 3-phosphate
CoA + ?
show the reaction diagram
Euglena sp.
-
-
-
?
acyl-[acyl-carrier protein] + 1-acyl-sn-glycerol 3-phosphate
ACP + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
-
involved in phospholipid biosynthesis in chloroplasts
-
?
arachidonoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-arachidonoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
arachidonoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-arachidonoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
arachidonoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-arachidonoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
arachidonoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-arachidonoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
arachidonoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-arachidonoyl-sn-glycerol 3-phosphate
show the reaction diagram
Q99943
-
-
?
arachidonoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-arachidonoyl-sn-glycerol 3-phosphate
show the reaction diagram
C4B4E7
-
-
-
?
arachidonoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-arachidonoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
arachidonoyl-CoA is a poor substrate
-
?
arachidonoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-arachidonoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
arachidonoyl-CoA is a poor substrate
-
?
arachidonoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-arachidonoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
arachidonoyl-CoA is a poor substrate
-
?
arachidonoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-arachidonoyl-sn-glycerol 3-phosphate
show the reaction diagram
Mus musculus C57BL/6J
C4B4E7
-
-
-
?
arachidonoyl-CoA + 1-arachidonoyl-2-lyso-sn-phosphatidylcholine
CoA + 1,2-diarachidonoyl-sn-phosphatidylcholine
show the reaction diagram
Q9DBL9
-
-
-
?
arachidonoyl-CoA + 1-arachidonoyl-sn-glycerol 3-phosphate
CoA + 1,2-diarachidonoyl-sn-glycerol 3-phosphate
show the reaction diagram
Q9DBL9
slight preference for 1-oleoyl lysophosphatidic acid over 1-palmitoyl, 1-stearoyl, or 1-arachidonoyl lysophosphatidic acid
-
-
?
arachidonoyl-CoA + 1-octadecenyl-2-lysophosphatidylinositol
CoA + 1-octadecenyl-2-arachidonoylphosphatidylinositol
show the reaction diagram
C4B4E7
-
-
-
?
arachidonoyl-CoA + 1-oleoyl-2-lysophosphatidylcholine
CoA + 1-oleoyl-2-arachidonoylphosphatidylcholine
show the reaction diagram
C4B4E7
-
-
-
?
arachidonoyl-CoA + 1-oleoyl-glycerol-3-phosphate
1-oleoyl-2-arachidonoyl-glycerol-3-phosphate + CoA
show the reaction diagram
-
-
-
-
?
arachidonoyl-CoA + 1-oleoyl-sn-glycerol 3-phosphate
CoA + 1-oleoyl-2-arachidonoyl-sn-glycerol 3-phosphate
show the reaction diagram
Q9NRZ7, Q9NUQ2
about half of the rate with oleoyl-CoA
-
-
?
arachidonoyl-CoA + 1-oleoyl-sn-glycerol 3-phosphate
CoA + 1-oleoyl-2-arachidonoyl-sn-glycerol 3-phosphate
show the reaction diagram
Q9DBL9
and oleoyl-CoA, preferred substrates
-
-
?
arachidonoyl-CoA + 1-oleoyl-sn-glycerol 3-phosphate
CoA + 1-oleoyl-2-arachidonoyl-sn-glycerol 3-phosphate
show the reaction diagram
C4B4E7
minor substrate
-
-
?
arachidonoyl-CoA + 1-oleoyl-sn-lysophosphatidylcholine
CoA + 1-oleoyl-2-arachidonoylphosphatidylcholine
show the reaction diagram
Q9DBL9
-
-
-
?
arachidonoyl-CoA + 1-palmitoyl-2-lysophosphatidylinositol
CoA + 1-palmitoyl-2-arachidonoylphosphatidylinositol
show the reaction diagram
C4B4E7
-
-
-
?
arachidonoyl-CoA + 1-palmitoyl-sn-glycerol 3-phosphate
CoA + 1-palmitoyl-2-arachidonoyl-sn-glycerol 3-phosphate
show the reaction diagram
Q9DBL9
slight preference for 1-oleoyl lysophosphatidic acid over 1-palmitoyl, 1-stearoyl, or 1-arachidonoyl lysophosphatidic acid
-
-
?
arachidonoyl-CoA + 1-palmitoyl-sn-lysophosphatidylcholine
CoA + 1-palmitoyl-2-arachidonoylphosphatidylcholine
show the reaction diagram
Q9DBL9
-
-
-
?
arachidonoyl-CoA + 1-stearoyl-2-lysophosphatidic acid
CoA + 1-stearoyl-2-arachidonoyl-sn-glycerol 3-phosphate
show the reaction diagram
C4B4E7
-
-
-
?
arachidonoyl-CoA + 1-stearoyl-sn-glycerol 3-phosphate
CoA + 1-oleoyl-2-stearoyl-sn-glycerol 3-phosphate
show the reaction diagram
Q9DBL9
slight preference for 1-oleoyl lysophosphatidic acid over 1-palmitoyl, 1-stearoyl, or 1-arachidonoyl lysophosphatidic acid
-
-
?
arachidonoyl-CoA + 1-stearoyl-sn-lysophosphatidylcholine
CoA + 1-stearoyl-2-arachidonoyl-sn-glycerol 3-phosphocholine
show the reaction diagram
Q9DBL9
-
-
-
?
arachidoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-arachidoyl-sn-glycerol 3-phosphate
show the reaction diagram
Q99943
-
-
?
behenoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-behenoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
capryl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-capryl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
cis-11-eicosenoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-cis-11 eicosenoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
docosahexaenoyl-CoA + 1-oleoyl-sn-glycerol 3-phosphate
CoA + 1-oleoyl-2-decosahexaenoyl-sn-glycerol 3-phosphate
show the reaction diagram
C4B4E7
best substrates
-
-
?
eicosatetraenoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-eicosatetraenoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
erucoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-erucoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
erucoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-erucoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
erucoyl-CoA is a poor substrate
-
?
erucoyl-CoA + 1-erucoyl-sn-glycerol-3-phosphate
CoA + 1,2-dierucoyl-sn-glycerol 3-phosphate
show the reaction diagram
Q40119, Q42870
-
-
?
gondoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-gondoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
gondoyl-CoA is utilized less efficiently for the reaction than oleoyl-CoA
-
?
heptadecanoyl-CoA + 1-oleoyl-sn-glycerol 3-phosphate
CoA + 1-oleoyl-2-heptadecanoyl-sn-glycerol 3-phosphate
show the reaction diagram
Q7L5N7
-
-
-
?
heptadecanoyl-CoA + 1-oleoyl-sn-glycerol 3-phosphate
CoA + 1-oleoyl-2-heptadecanoyl-sn-glycerol 3-phosphate
show the reaction diagram
Q9NRZ7, Q9NUQ2
about half of the rate with oleoyl-CoA
-
-
?
lauroyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-lauroyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
lauroyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-lauroyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
lauroyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-lauroyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
lauroyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-lauroyl-sn-glycerol 3-phosphate
show the reaction diagram
Q99943
-
-
?
lauroyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-lauroyl-sn-glycerol 3-phosphate
show the reaction diagram
-
transgenic seeds: preferred substrate
-
?
lauroyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-lauroyl-sn-glycerol 3-phosphate
show the reaction diagram
-
lauroyl-CoA is a poor substrate
-
?
lauroyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-lauroyl-sn-glycerol 3-phosphate
show the reaction diagram
-
lauroyl-CoA is a poor substrate
-
?
lauroyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-lauroyl-sn-glycerol 3-phosphate
show the reaction diagram
-
lauroyl-CoA is a poor substrate
-
?
lignoceroyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-lignoceroyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
lignoceroyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-lignoceroyl-sn-glycerol 3-phosphate
show the reaction diagram
Q99943
-
-
?
linolenoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-linolenoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
linolenoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-linolenoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
linolenoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-linolenoyl-sn-glycerol 3-phosphate
show the reaction diagram
Q99943
-
-
?
linoleoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-linoleoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
linoleoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-linoleoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
-
?
linoleoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-linoleoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
linoleoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-linoleoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
linoleoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-linoleoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
linoleoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-linoleoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
linoleoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-linoleoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
linoleoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-linoleoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
linoleoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-linoleoyl-sn-glycerol 3-phosphate
show the reaction diagram
Q99943
-
-
?
linoleoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-linoleoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
linoleoyl-CoA is a good substrate but is utilized less efficiently than oleoyl-CoA
-
?
linoleoyl-CoA + 1-oleoyl-sn-glycerol 3-phosphate
CoA + 1-oleoyl-2-linoleoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
r
linoleoyl-CoA + 1-oleoyl-sn-glycerol 3-phosphate
CoA + 1-oleoyl-2-linoleoyl-sn-glycerol 3-phosphate
show the reaction diagram
Q7L5N7
-
-
-
?
linoleoyl-CoA + 1-oleoyl-sn-glycerol 3-phosphate
CoA + 1-oleoyl-2-linoleoyl-sn-glycerol 3-phosphate
show the reaction diagram
Q9NRZ7, Q9NUQ2
about half of the rate with oleoyl-CoA
-
-
?
myristoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-myristoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
myristoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-myristoyl-sn-glycerol 3-phosphate
show the reaction diagram
Q99943
-
-
?
myristoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-myristoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
myristoyl-CoA is a poor substrate
-
?
myristoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-myristoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
myristoyl-CoA is a poor substrate
-
?
myristoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-myristoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
myristoyl-CoA is a poor substrate
-
?
myristoyl-CoA + 1-oleoyl-sn-glycerol 3-phosphate
CoA + 1-oleoyl-2-myristoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
about 25% of the activity with oleoyl-CoA by AGPAT10/GPAT3
-
-
?
oleoyl-ACP + sn-1-oleoylglycerol 3-phosphate
ACP + sn-1,2-dioleoylglycerol 3-phosphate
show the reaction diagram
-
microsomal fractions from developing, transgenic seeds but not of untransformed plants
-
?
oleoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-oleoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
oleoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-oleoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
oleoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-oleoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
oleoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-oleoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
oleoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-oleoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
oleoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-oleoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
oleoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-oleoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
oleoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-oleoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
oleoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-oleoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
oleoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-oleoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
oleoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-oleoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
oleoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-oleoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
oleoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-oleoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
oleoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-oleoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
oleoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-oleoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
oleoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-oleoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
oleoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-oleoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
oleoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-oleoyl-sn-glycerol 3-phosphate
show the reaction diagram
Euglena sp.
-
-
-
?
oleoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-oleoyl-sn-glycerol 3-phosphate
show the reaction diagram
Q40119, Q42870
-
-
?
oleoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-oleoyl-sn-glycerol 3-phosphate
show the reaction diagram
Q99943
-
-
?
oleoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-oleoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
twice as good as palmitoyl-CoA
-
?
oleoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-oleoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
oleoyl-CoA is the best substrate, both membrane-bound and partially purified enzyme
-
?
oleoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-oleoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
is the most effective acyl donor
-
?
oleoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-oleoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
high selectivity for oleate
-
?
oleoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-oleoyl-sn-glycerol 3-phosphate
show the reaction diagram
Q9XFW4
i.e. lysophosphatidic acid
i.e. phosphatidic acid
-
?
oleoyl-CoA + 1-arachidonoyl-sn-lysophosphatidylcholine
CoA + 1-arachidonoyl-2-oleoyl-sn-glycero-3-phosphocholine
show the reaction diagram
Q9DBL9
-
-
-
?
oleoyl-CoA + 1-oleoyl-2-lysophosphatidylcholine
CoA + 1,2-dioleoyl-sn-glycero-3-phosphocholine
show the reaction diagram
Q9DBL9
-
-
-
?
oleoyl-CoA + 1-oleoyl-2-lysophosphatidylcholine
CoA + 1,2-dioleoyl-sn-glycero-3-phosphocholine
show the reaction diagram
-
about 10% of the activity with 1-oleoyl-2-lysophopshatidic acid by AGPAT10/GPAT3
-
-
?
oleoyl-CoA + 1-oleoyl-2-lysophosphatidylethanolamine
CoA + 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine
show the reaction diagram
-
about 10% of the activity with 1-oleoyl-2-lysophopshatidic acid by AGPAT10/GPAT3
-
-
?
oleoyl-CoA + 1-oleoyl-2-lysophosphatidylglycerol
CoA + 1,2-dioleoyl-sn-glycero-3-phosphate
show the reaction diagram
-
about 10% of the activity with 1-oleoyl-2-lysophopshatidic acid by AGPAT10/GPAT3
-
-
?
oleoyl-CoA + 1-oleoyl-2-lysophosphatidylinositol
CoA + 1,2-dioleoyl-sn-phosphatidylinositol
show the reaction diagram
-
about 10% of the activity with 1-oleoyl-2-lysophopshatidic acid by AGPAT10/GPAT3
-
-
?
oleoyl-CoA + 1-oleoyl-2-lysophosphatidylserine
CoA + 1,2-dioleoyl-sn-glycerol-3-phospho-L-serine
show the reaction diagram
-
about 10% of the activity with 1-oleoyl-2-lysophopshatidic acid by AGPAT10/GPAT3
-
-
?
oleoyl-CoA + 1-oleoyl-sn-glycerol 3-phosphate
CoA + 1,2-dioleoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
assayed with BAT2 protein
-
?
oleoyl-CoA + 1-oleoyl-sn-glycerol 3-phosphate
CoA + 1,2-dioleoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
oleoyl-CoA and 1-oleoyl-sn-glycerol 3-phosphate are the preferred substrates of AGPAT10/GPAT3
-
-
?
oleoyl-CoA + 1-oleoyl-sn-glycerol 3-phosphate
CoA + 1,2-dioleoyl-sn-glycerol 3-phosphate
show the reaction diagram
Q9DBL9
and arachidonoyl-CoA, preferred substrates. Slight preference for 1-oleoyl lysophosphatidic acid over 1-palmitoyl, 1-stearoyl, or 1-arachidonoyl lysophosphatidic acid
-
-
?
oleoyl-CoA + 1-oleoyl-sn-glycerol 3-phosphate
CoA + 1,2-dioleoyl-sn-glycerol 3-phosphate
show the reaction diagram
O15120
both substrates are preferred
-
-
?
oleoyl-CoA + 1-oleoyl-sn-glycerol 3-phosphate
CoA + 1,2-dioleoyl-sn-glycerol 3-phosphate
show the reaction diagram
Q7L5N7
both substrates are preferred
-
-
?
oleoyl-CoA + 1-oleoyl-sn-glycerol 3-phosphate
CoA + 1,2-dioleoyl-sn-glycerol 3-phosphate
show the reaction diagram
Q9NRZ7, Q9NUQ2
both substrates are preferred
-
-
?
oleoyl-CoA + 1-oleoyl-sn-glycerol 3-phosphate
CoA + 1,2-dioleoyl-sn-glycerol 3-phosphate
show the reaction diagram
C4B4E7
minor substrate
-
-
?
oleoyl-CoA + 1-oleoyl-sn-glycerol-3-phosphate
CoA + 1,2-dioleoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
-
?
oleoyl-CoA + 1-oleoyl-sn-glycerol-3-phosphate
CoA + 1,2-dioleoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
substrate preference higher for oleoyl-CoA than for palmitoyl-CoA and stearoyl-CoA
-
-
?
oleoyl-CoA + 1-oleoyl-sn-glycerol-3-phosphate
CoA + 1,2-dioleoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
substrate preference higher for oleoyl-CoA than for palmitoyl-CoA and stearoyl-CoA
-
-
?
oleoyl-CoA + 1-palmitoyl-sn-glycerol 3-phosphate
CoA + 1-palmitoyl-2-oleoyl-sn-glycerol 3-phosphate
show the reaction diagram
Q06508
-
-
-
?
oleoyl-CoA + 1-palmitoyl-sn-lysophosphatidylcholine
CoA + 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine
show the reaction diagram
Q9DBL9
-
-
-
?
oleoyl-CoA + 1-stearoyl-sn-lysophosphatidylcholine
CoA + 1-stearoyl-2-oleoyl-sn-glycero-3-phosphocholine
show the reaction diagram
Q9DBL9
-
-
-
?
oleoyl-CoA + lysophosphatidic acid
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
-
?
palmitoleoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-palmitoleoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
palmitoleoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-palmitoleoyl-sn-glycerol 3-phosphate
show the reaction diagram
Q59618
-
-
?
palmitoleoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-palmitoleoyl-sn-glycerol 3-phosphate
show the reaction diagram
Q59601
-
-
?
palmitoleoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-palmitoleoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
palmitoleoyl-CoA is a good substrate but is utilized less efficiently than oleoyl-CoA
-
?
palmitoleoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-palmitoleoyl-sn-glycerol 3-phosphate
show the reaction diagram
Q99943
palmitoleoyl-CoA is a good substrate
-
?
palmitoyl-ACP + 1-acyl-sn-glycerol 3-phosphate
ACP + 1-acyl-2-palmitoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
palmitoyl-ACP + 1-acyl-sn-glycerol 3-phosphate
ACP + 1-acyl-2-palmitoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
palmitoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-palmitoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
palmitoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-palmitoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
palmitoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-palmitoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
palmitoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-palmitoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
palmitoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-palmitoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
palmitoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-palmitoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
palmitoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-palmitoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
palmitoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-palmitoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
-
?
palmitoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-palmitoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
palmitoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-palmitoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
palmitoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-palmitoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
palmitoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-palmitoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
palmitoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-palmitoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
palmitoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-palmitoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
palmitoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-palmitoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
palmitoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-palmitoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
palmitoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-palmitoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
palmitoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-palmitoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
palmitoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-palmitoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
palmitoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-palmitoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
palmitoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-palmitoyl-sn-glycerol 3-phosphate
show the reaction diagram
Euglena sp.
-
-
-
?
palmitoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-palmitoyl-sn-glycerol 3-phosphate
show the reaction diagram
Q99943
-
-
?
palmitoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-palmitoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
maximum activity is obtained with palmitoyl-CoA at a concentration of 0.002-0.003 mM
-
?
palmitoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-palmitoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
palmitoyl-CoA is the best substrate
-
?
palmitoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-palmitoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
palmitoyl-CoA is a poor substrate
-
?
palmitoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-palmitoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
palmitoyl-CoA is a poor substrate
-
?
palmitoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-palmitoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
acyltransferase motifs I-IV analyzed by sidechain modification, substrate accessibility to the catalytic domain analyzed by competition assay
-
-
?
palmitoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-palmitoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
substrate preference lower than for oleoyl-CoA
-
-
?
palmitoyl-CoA + 1-oleoyl-sn-glycerol 3-phosphate
CoA + 1-oleoyl-2-palmitoyl-sn-glycerol 3-phosphate
show the reaction diagram
Q7L5N7
-
-
-
?
palmitoyl-CoA + 1-oleoyl-sn-glycerol 3-phosphate
CoA + 1-oleoyl-2-palmitoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
assayed with BAT2 protein, palmitoyl-CoA is the preferred substrate
-
?
palmitoyl-CoA + 1-oleoyl-sn-glycerol 3-phosphate
CoA + 1-oleoyl-2-palmitoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
about 25% of the activity with oleoyl-CoA by AGPAT10/GPAT3
-
-
?
palmitoyl-CoA + 1-oleoyl-sn-glycerol 3-phosphate
CoA + 1-oleoyl-2-palmitoyl-sn-glycerol 3-phosphate
show the reaction diagram
Q9NRZ7, Q9NUQ2
about 40% of the rate with oleoyl-CoA
-
-
?
pentadecanoyl-CoA + 1-oleoyl-sn-glycerol 3-phosphate
CoA + 1-oleoyl-2-pentadecanoyl-sn-glycerol 3-phosphate
show the reaction diagram
O15120
50% of the rate with oleoyl-CoA and linoleoyl-CoA, rate is similar to oleoyl-CoA and linoleoyl-CoA
-
-
?
stearoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-stearoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
-
?
stearoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-stearoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
stearoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-stearoyl-sn-glycerol 3-phosphate
show the reaction diagram
Q99943
-
-
?
stearoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-stearoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
stearoyl-CoA is a poor substrate
-
?
stearoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-stearoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
stearoyl-CoA is a poor substrate
-
?
stearoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-stearoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
stearoyl-CoA is a poor substrate
-
?
stearoyl-CoA + 1-oleoyl-sn-glycerol 3-phosphate
CoA + 1-oleoyl-2-stearoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
about 25% of the activity with oleoyl-CoA by AGPAT10/GPAT3
-
-
?
stearoyl-CoA + 1-oleoyl-sn-glycerol 3-phosphate
CoA + 1-oleoyl-2-stearoyl-sn-glycerol 3-phosphate
show the reaction diagram
Q9NRZ7, Q9NUQ2
about 40% of the rate with oleoyl-CoA
-
-
?
linoleoyl-CoA + 1-oleoyl-sn-glycerol 3-phosphate
CoA + 1-oleoyl-2-linoleoyl-sn-glycerol 3-phosphate
show the reaction diagram
O15120
linoleoyl-CoA is second best acyl-CoA substrate
-
-
?
additional information
?
-
C4B4E7
substrate specificity, overview
-
-
-
additional information
?
-
-
the enzyme can utilize various saturated and unsaturated acyl-CoAs at comparable rates, unsaturated fatty acyl-CoAs with cis- or trans-configuration are slightly better substrates than saturated acyl-CoAs
-
-
-
additional information
?
-
-
the enzyme utilizes both saturated and unsaturated acyl-CoA as an acyl-donor but does not catalyse the transfer of acyl moiety to lysophosphatidylcholine or lyso-platelet-activating factor
-
-
-
additional information
?
-
-
effect of acyl-CoA concentrations on enzyme reaction
-
-
-
additional information
?
-
-
acyl-CoAs containing 10:0, 12:0 and 14:0 acyl groups are the preferred acyl-donor substrates, acyl-ACPs are not utilized. There is a slight preference for 12:0-lysophosphatidic acid over 18:1-lysophosphatidic acid as acceptor substrate
-
-
-
additional information
?
-
-
no acceptors are 1-acyl-sn-glycero-3-phosphoethanolamine, 1-acyl-sn-glycero-3-phosphomonomethylethanolamine, 1-acyl-sn-glycero-3-phosphodimethylethanolamine or 1-acyl-sn-glycero-3-phosphocholine
-
-
-
additional information
?
-
-
the enzyme uses preferentially those acyl donors and acceptors, which contain oleate, specificity and selectivity experiments
-
-
-
additional information
?
-
-
the enzyme uses preferably ACP-thioesters for the acylation of 1-acylglycerol 3-phosphate, the enzyme does not accept glycerol 3-phosphate as acyl-acceptor
-
-
-
additional information
?
-
-
the enzyme shows higher activities with acyl-CoA than with the corresponding acyl-(acyl carrier protein) thioesters, the enzyme is more active on unsaturated than on saturated acyl-CoA thioesters
-
-
-
additional information
?
-
-
no acyl-donors are butyryl-CoA and hexanoyl-CoA
-
-
-
additional information
?
-
-
very broad specificity for acyl-CoAs, the enzyme does not seem to discriminate strictly the cis and trans configurations or the position of double bond in the fatty acyl moiety
-
-
-
additional information
?
-
-
description of a direct nonchromatographic assay for the enzyme
-
-
-
additional information
?
-
-
no acyl-donors are coenzyme A derivatives
-
-
-
additional information
?
-
-
no acyl-donors are coenzyme A derivatives
-
-
-
additional information
?
-
-
the enzyme exhibits a considerable selectivity for monoenoic and dienoic fatty acyl-CoA thioesters
-
-
-
additional information
?
-
-
lysophospholipids such as 1-acyl-sn-glycero-3-phosphocholine, 1-acyl-sn-glycero-3-phosphoethanolamine, 1-acyl-sn-glycero-3-phosphoinositol and lysoplatelet-activating factor do not serve as acyl acceptor substrates. Free fatty acids do not serve as acyl donors. The enzyme shows a broad specificity for acyl-CoAs, reverse reaction of the enzyme: desulfo-CoA and dephospho-COA do not serve as substrates for acyl-CoA synthesis
-
-
-
additional information
?
-
Q99943
the enzyme has affinity for fatty acids of acyl chain lengths from 12 to 18 carbons with a slight dependence on the degree of saturation of the fatty acid. However the enzyme does not incorporate long chain fatty acids like C20:0 and C24:0 unless they are saturated
-
-
-
additional information
?
-
-
no acyl acceptor is glycerol 3-phosphate
-
-
-
additional information
?
-
-
no acyl acceptor is glycerol 3-phosphate
-
-
-
additional information
?
-
-
expression of a 299-amino acid protein with enzyme activity alters the enzyme substrate specificity profile of transgenic canola seeds
-
-
-
additional information
?
-
-
the chain length specificity of the enzyme in decreasing order is as follows: C16, C14, C12, C10, C8
-
-
-
additional information
?
-
-
effect of variable ratios of oleoyl-CoA to palmitoyl-CoA on the reaction
-
-
-
additional information
?
-
-
the microsomal enzyme displays a specificity for unsaturated C18-CoA thioesters and this fatty-acid specificity is not altered in the course of the purification procedure
-
-
-
additional information
?
-
-
the enzyme system shows relatively broad specificity for saturated and unsaturated fatty acids, with 14- to 20-carbon chains. Time courses fo the acylations of 1-acyl-glycerophosphate in the presence of equimolar mixtures of oleoyl-CoA and arachidonoyl-CoA
-
-
-
additional information
?
-
-
1-acyl-sn-glycerol 3-phosphate is readily acylated by unsaturated and saturated acyl-CoAs
-
-
-
additional information
?
-
-
lysophosphatidic acid, synthesized by EC 2.3.1.15, is utilized by the enzyme without prior mixing with the total membrane-associated pool of lysophosphatidic acid, and suggest a close proximity of the two enzymes in native E. coli membranes. This property of the acyltransferases is lost upon separation and reconstitution of enzyme activities
-
-
-
additional information
?
-
-
an introduced chimeric enzyme gene of E. coli alters the stereochemical composition of transgenic HEAR oil by preferentially directing cis-11 eicosenoic acid to its sn-2 position
-
-
-
additional information
?
-
-
the enzyme controls phosphatidic acid biosynthesis in the plastid
-
-
-
additional information
?
-
Q40119, Q42870
pLAT2 may be necessary only for specific storage triacylglycerol biosynthesis in developing seed
-
-
-
additional information
?
-
Q40119, Q42870
LPAT1 may be an enzyme required for membrane phospholipid biosynthesis in all tissues
-
-
-
additional information
?
-
-
the enzyme has medium chain length substrate specificity appropriate to the biosynthesis of coconut oil
-
-
-
additional information
?
-
-
acyl-ACP rather than acyl-CoA is the physiological acyl donor for glycerolipid biosynthesis in chloroplasts
-
-
-
additional information
?
-
-
acyl-CoA thioesters can be considered as the physiological substrates of the microsomal enzyme
-
-
-
additional information
?
-
-
1-palmitoyl- and 1-oleoyl-sn-glycerol 3-phosphate are probably the substrates for the enzyme in vivo, since palmitic and oleic acids are the major fatty acid components at the sn-1 position of cow milk triacylglycerols. The specificity of the enzyme plays a major role in determining the fatty acid composition at the sn-2 position of cow milk triacylglycerols, though the influence of other factors such as the in vivo concentration of the acyl-CoAs and the specificities of the monoacylglycerol acyltransferases cannot be ruled out
-
-
-
additional information
?
-
-
AGPAT8 shows moderate acyltransferase activity with oleoyl-CoA but lacks acyl-CoA:lysocardiolipin acyltransferase activity, human AGPAT2, acylation at the sn-2 position with wild-type AGPAT8 is similar for the LPA species containing oleoyl, linoleoyl, linolenoyl, and palmitoyl fatty acids at the sn-1 position, LPA species containing myristoyl, arachidoyl, arachidonoyl are not preferred, substrate specificity in vivo, overview
-
-
-
additional information
?
-
Q8GXU8, Q8L4Y2, Q8LG50, Q9LHN4, Q9SYC8
the endoplasmic reticulum-located LPAT2 is essential for female but not male gametophyte development in Arabidopsis thaliana, LPAT2 co-localizes with calreticulin in the endoplasmic reticulum
-
-
-
additional information
?
-
-
developmental and substrate specificity, overview
-
-
-
additional information
?
-
-
sn-1-acyl-lysophosphatidic acid and acly-CoA specficity, AGPAT8 is unable to use arachidonoyl-CoA as the acyl donor, overview
-
-
-
additional information
?
-
Q9DBL9
CGI-58 is a CoA-dependent lysophosphatidic acid acyltransferase that channels fatty acids released from the hydrolysis of stored triacylglycerols into phospholipids, overview
-
-
-
additional information
?
-
-
CGI-58 is a CoA-dependent lysophosphatidic acid acyltransferase that channels fatty acids released from the hydrolysis of stored triacylglycerols into phospholipids, overview
-
-
-
additional information
?
-
-
fatty acid and phospholipid profiles, overview
-
-
-
additional information
?
-
-
LPAAT3 regulates Golgi membrane tubule formation, trafficking, and structure by altering phospholipids and lysophospholipids. LPAAT3 alters ERGIC-53 distribution, a p58 receptor which is a lectin receptor that traffics between the cis-Golgi and the endoplasmic reticulum
-
-
-
additional information
?
-
C4B4E7
mouse LPAAT3, previously known as mouse AGPAT3, possesses strong LPAAT activity and modest lysophosphatidylinositol acyltransferase activity with a clear preference for arachidonoyl-CoA as a donor, overview
-
-
-
additional information
?
-
-
substrate specificity of AGPAT10/GPAT3, overview
-
-
-
additional information
?
-
-
the CGI-58 carboxyl terminus includes a highly conserved consensus sequence, HXXXXD, required for acyltransferase activity
-
-
-
additional information
?
-
Q9DBL9
the recombinant CoA-dependent His-tagged CGI-58 is active with lysophosphatidic acid, but not with other lysophospholipid or neutral glycerolipid acceptors
-
-
-
additional information
?
-
Q7L5N7
isoform AGPAT11 shows broad preferences for the lysophosphatidic acid containing saturated fatty acids C16:0-C18:0, including lysophosphatidic acid containing oleic acid. Lysophosphatidic acid with fatty acid C20:4 is acylated at only about one-half maximal rate compared with others. The preferred acyl donor follows the decreasing order of C18:1,C16:0, C18:2, C17:0. Fatty acyl-CoA consisting of short-chain fatty acids C8:0 to C13:0 and very long-chain from C20:0 to C26:0 are not substrates. Enzyme does not display glycerol-3-phosphate acyltransferase activity
-
-
-
additional information
?
-
Q9NRZ7, Q9NUQ2
isoform AGPAT3 shows broad preference for lysophosphatidic acids containing saturated or unsaturated fatty acids C16:0-C20:4 and significant esterification of lysophosphatidylinositol in the presence of oleoyl-CoA
-
-
-
additional information
?
-
Q9NRZ7, Q9NUQ2
isoform AGPAT5 demonstrates significant acyltransferase activity toward lysophosphatidylethanolamine in the presence of oleoyl-CoA
-
-
-
additional information
?
-
C4B4E7
isoform LPAAT3 shows a clear preference for 18:1-lyso-PA as acyl acceptor and 22:6-CoA as acyl donor compared to other substrate combinations tested. The respective product sn-1-18:1-sn-2-22:6-phosphatidic acid is almost exclusively formed by LPAAT3. The enzyme shows Michaelis-Menten rate behavior toward 22:6-CoA in the presence of sn-1-18:1-lyso-phosphatidic acid
-
-
-
additional information
?
-
O15120
no substrates: acyl-CoA with fatty acids shorter than C14:0 or longer than C20:0, whether saturated or unsaturated, no substrates: acyl-CoA with fatty acids shorter than C14:0 or longer than C20:0, whether saturated or unsaturated. Isoform AGPAT2 is rather restrictive in using 1-oleoyl-sn-glycerol 3-phosphate as acceptor substrate
-
-
-
additional information
?
-
Q06508
no substrates: lysophosphatidyl choline, lysophosphatidyl ethanolamine, lysophosphatidyl glycerol, and lysophosphatidyl inositol
-
-
-
additional information
?
-
Mus musculus C57BL/6J
C4B4E7
substrate specificity, overview, mouse LPAAT3, previously known as mouse AGPAT3, possesses strong LPAAT activity and modest lysophosphatidylinositol acyltransferase activity with a clear preference for arachidonoyl-CoA as a donor, overview
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
-
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
-
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
-
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
-
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
Q643R3
-
-
-
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
C4B4E7
-
-
-
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
Q9DBL9
i.e. lysophosphatidic acid
i.e. phosphatidic acid
-
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
Q9XFW4
i.e. lysophosphatidic acid
i.e. phosphatidic acid
-
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
O22975
i.e. lysophosphatidic acid
i.e. phosphatidic acid
-
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
-
involved in phospholipid biosynthesis
-
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
-
involved in phospholipid biosynthesis
-
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
-
involved in phospholipid biosynthesis
-
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
-
involved in phospholipid biosynthesis
-
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
-
involved in phospholipid biosynthesis
-
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
-
involved in phospholipid biosynthesis
-
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
-
involved in phospholipid biosynthesis
-
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
-
pivotal enzyme controlling the metabolic flow of lysophosphatidic acid into different phosphatidic acids in diverse tissues, enzyme is essential in embryo development
-
-
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
-
reduction in AGPAT2 activity underlies the loss of adipose tissue in congenital generalized lipodystrophy
-
-
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
O15120
AGPAT2 catalyzes acylation of lysophosphatidic acid to phosphatidic acid, a precursor for both triacylglycerol and phospholipid synthesis, isozyme AGPAT2 plays a regulatory role in adipocyte differentiation
-
-
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
-
the enzyme catalyzes the second acylations in the glycerol-3-phosphate pathway
-
-
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
-
the expression of AGPATs is linked to skin barrier requirements
-
-
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
Q8NF37
activity and expression pattern of AGPAT9 in lung and spleen implicates role in the biosynthesis of phospholipids and triglycerides in these tissues
-
-
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
P33333, Q08548
acyltranferase activity by Slc1p and Slc4p
-
-
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
P33333, Q08548
Slc1p and Slc4p both active as acyltransferases, involved in fatty acid exchange of mature glycerophospholipids
-
-
?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
Mus musculus C57BL/6J
C4B4E7
-
-
-
?
acyl-[acyl-carrier protein] + 1-acyl-sn-glycerol 3-phosphate
ACP + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
-
involved in phospholipid biosynthesis in chloroplasts
-
?
arachidonoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-arachidonoyl-sn-glycerol 3-phosphate
show the reaction diagram
Mus musculus, Mus musculus C57BL/6J
C4B4E7
-
-
-
?
additional information
?
-
-
the enzyme controls phosphatidic acid biosynthesis in the plastid
-
-
-
additional information
?
-
Q40119, Q42870
pLAT2 may be necessary only for specific storage triacylglycerol biosynthesis in developing seed
-
-
-
additional information
?
-
Q40119, Q42870
LPAT1 may be an enzyme required for membrane phospholipid biosynthesis in all tissues
-
-
-
additional information
?
-
-
the enzyme has medium chain length substrate specificity appropriate to the biosynthesis of coconut oil
-
-
-
additional information
?
-
-
acyl-ACP rather than acyl-CoA is the physiological acyl donor for glycerolipid biosynthesis in chloroplasts
-
-
-
additional information
?
-
-
acyl-CoA thioesters can be considered as the physiological substrates of the microsomal enzyme
-
-
-
additional information
?
-
-
1-palmitoyl- and 1-oleoyl-sn-glycerol 3-phosphate are probably the substrates for the enzyme in vivo, since palmitic and oleic acids are the major fatty acid components at the sn-1 position of cow milk triacylglycerols. The specificity of the enzyme plays a major role in determining the fatty acid composition at the sn-2 position of cow milk triacylglycerols, though the influence of other factors such as the in vivo concentration of the acyl-CoAs and the specificities of the monoacylglycerol acyltransferases cannot be ruled out
-
-
-
additional information
?
-
-
AGPAT8 shows moderate acyltransferase activity with oleoyl-CoA but lacks acyl-CoA:lysocardiolipin acyltransferase activity, human AGPAT2, acylation at the sn-2 position with wild-type AGPAT8 is similar for the LPA species containing oleoyl, linoleoyl, linolenoyl, and palmitoyl fatty acids at the sn-1 position, LPA species containing myristoyl, arachidoyl, arachidonoyl are not preferred, substrate specificity in vivo, overview
-
-
-
additional information
?
-
Q8GXU8, Q8L4Y2, Q8LG50, Q9LHN4, Q9SYC8
the endoplasmic reticulum-located LPAT2 is essential for female but not male gametophyte development in Arabidopsis thaliana, LPAT2 co-localizes with calreticulin in the endoplasmic reticulum
-
-
-
additional information
?
-
Q9DBL9
CGI-58 is a CoA-dependent lysophosphatidic acid acyltransferase that channels fatty acids released from the hydrolysis of stored triacylglycerols into phospholipids, overview
-
-
-
additional information
?
-
-
CGI-58 is a CoA-dependent lysophosphatidic acid acyltransferase that channels fatty acids released from the hydrolysis of stored triacylglycerols into phospholipids, overview
-
-
-
additional information
?
-
-
fatty acid and phospholipid profiles, overview
-
-
-
additional information
?
-
-
LPAAT3 regulates Golgi membrane tubule formation, trafficking, and structure by altering phospholipids and lysophospholipids. LPAAT3 alters ERGIC-53 distribution, a p58 receptor which is a lectin receptor that traffics between the cis-Golgi and the endoplasmic reticulum
-
-
-
additional information
?
-
Mus musculus, Mus musculus C57BL/6J
C4B4E7
mouse LPAAT3, previously known as mouse AGPAT3, possesses strong LPAAT activity and modest lysophosphatidylinositol acyltransferase activity with a clear preference for arachidonoyl-CoA as a donor, overview
-
-
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Mg2+
Q9XFW4
-
additional information
-
no Mg2+-requirement
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
1-acyl-sn-glycero 3-phosphate
-
high concentration
1-palmitoyl-sn-glycerol 3-phosphate
-
above 0.05 mM
2-mercaptoethanol
-
above 5 mM
2-[3,5-Di-(t-butyl-4-hydroxyphenyl)thio]hexanate
-
i.e. DH990, weak
Ca2+
-
50% inhibition above 10 mM
CaCl2
Q9DBL9
1 microM, 60% inhibition. Addition of EDTA restores activity
CHAPS
-
above 0.4% w/v
CHAPS
-
above 0.1%
CI-976
-
inhibits LPAAT and stimulates Golgi tubules
lysophosphatidylcholine
-
inhibits 68% of the enzyme activity at 30 microM
MgCl2
-
at high concentrations
MgCl2
-
50% inhibition above 10 mM
MgCl2
Q9DBL9
1 microM, 35% inhibition. Addition of EDTA restores activity
N-ethylmaleimide
Euglena sp.
-
-
NaCl
-
above 200 mM
Phospholipids
-
activity inhibited by high concentrations of
Sodium deoxycholate
-
concentration-dependent inactivation
stearoyl-CoA
-
strongly inhibitory at 0.002 mM
Triton X-100
-
0.5 mM and above
Triton X-100
-
concentration-dependent inactivation
Triton X-100
Q9DBL9
0.4 mM, inhibition
Mn2+
-
50% inhibition above 10 mM
additional information
-
no inhibition by sulfhydryl reagents, 1-acylglycerol 3-phosphoethanol, 1-acylglycerol 3-phosphoethanolamine, 1-acylglycerol 3-phosphodimethylethanolamine, 1-acylglycerol 3-choline
-
additional information
-
at low concentrations of enzyme protein, the enzyme is possibly inactivated by the detergent action of substrates
-
additional information
-
the enzyme can utilize the monomer form of long-chain acyl-CoA but it is inhibited by the micellar form of these substrates
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Bovine serum albumin
-
induces T-lymphocyte enzyme activity in the presence of palmitoyl-CoA but not oleoyl-CoA
-
isopropyl-beta-D-thiogalactopyranoside
-
induction of the enzyme gene
additional information
-
stimulation of the enzyme by incorporation of the enzyme into the artificial membrane vesicles or by stripping of CHAPS from the enzyme into mixed detergent-phospholipid micelles. There is a critical order in which assay components had to be combined to obtain maximum stimulation
-
additional information
-
isozyme AGPAT2 and AGPAT5 expression increase in parallel with both an increase in enzyme activity and permeability barrier formation late in rat epidermal development, acute permeability barrier disruption, by tape-stripping, leads to rapidly increased expression of isozymes AGPAT 1, 2, and 3, reversible by occlusion
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.0053
1-acyl-sn-glycerol 3-phosphate
-
I208A, mutant of human AGPAT1
0.0054
1-acyl-sn-glycerol 3-phosphate
-
P207L, mutant of human AGPAT1
0.0064
1-acyl-sn-glycerol 3-phosphate
-
human AGPAT1
0.024
1-acyl-sn-glycerol 3-phosphate
-
T180S, mutant of human AGPAT1
0.0342
1-acyl-sn-glycerol 3-phosphate
-
R149K, mutant of human AGPAT1
0.125
1-acyl-sn-glycerol 3-phosphate
-
in the incorporation of palmitate and linoleate
0.0048
1-oleoyl-sn-glycerol 3-phosphate
Q9NRZ7, Q9NUQ2
pH 7.4, 30C
0.0053
1-oleoyl-sn-glycerol 3-phosphate
-
with oleoyl-CoA
0.0055
1-oleoyl-sn-glycerol 3-phosphate
Q9NRZ7, Q9NUQ2
pH 7.4, 37C
0.0092
1-oleoyl-sn-glycerol 3-phosphate
-
pH 7.4, 37C, recombinant AGPAT10/GPAT3
0.018
1-oleoyl-sn-glycerol 3-phosphate
Q9DBL9
pH 7.5, 30C
0.003
1-palmitoyl-sn-glycerol 3-phosphate
-
with oleoyl-CoA
0.0138
Docosahexaenoyl-CoA
-
pH 7.5, 37C
0.0027
oleoyl-CoA
-
pH 7.4, 37C, recombinant AGPAT10/GPAT3
0.0028
oleoyl-CoA
-
-
0.004
oleoyl-CoA
Euglena sp.
-
-
0.0048
oleoyl-CoA
Q9DBL9
pH 7.5, 30C
0.014
oleoyl-CoA
-
-
0.0163
oleoyl-CoA
Q9NRZ7, Q9NUQ2
pH 7.4, 37C
0.0215
oleoyl-CoA
Q9NRZ7, Q9NUQ2
pH 7.4, 30C
0.0014
palmitoyl-CoA
-
-
0.0036
palmitoyl-CoA
-
R149K, mutant of human AGPAT1; T180S, mutant of human AGPAT1
0.004
palmitoyl-CoA
-
human AGPAT1
0.007
palmitoyl-CoA
Euglena sp.
-
-
0.0075
palmitoyl-CoA
-
I208A, mutant of human AGPAT1
0.0083
palmitoyl-CoA
-
P207L, mutant of human AGPAT1
0.012
palmitoyl-CoA
-
stearoyl-CoA as an acyl donor reveals only 33% of the activity observed under the standard assay conditions
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
22
oleoyl-CoA
-
pH 7.4, 37C, recombinant AGPAT10/GPAT3
281
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.0000108
-
inner mitochondrial membrane
0.0000192
-
mitochondria
0.0002742
-
outer mitochondrial membrane
0.0066
-
-
additional information
-
AGPAT8 has moderate acyltransferase activity, only 18.4% when compared to the other isoform, AGPAT2, using oleoyl-CoA as the acyl donor and 1-oleoyl-3-phosphate-glycerol as the acceptor
additional information
-
lipid content and fatty acid composition of triacylglycerols during seed development, overview
additional information
-
topology of motifs I and III confirmed by experiments with recombinant AGPAT1, catalytic roles of highly conserved residues in the four acyltransferase motifs analyzed by site-directed mutagenesis, sidechain modification of His104, Asp109, Phe146, Arg149, Glu178, Gly179, Thr180, Arg181 and Ile208 shows motif importance for catalysis, substrate accessibility to the catalytic domain analyzed by competition assay, catalytic domain not accessed by lysophosphatidic acid (LPA) with fatty acid chains shorter than 10 carbons, hydrophobicity of lysophosphatidic acid important, catalytic domain accessed by short chain acyl-CoAs but not as second substrate, motifs II and III involved in binding of lysophosphatidic acid, motifs I and IV involved in binding of acyl-CoA
additional information
P33333, Q08548
deletion of Slc1p alone not lethal, microsomal enzyme activity not completely lost, evidence for additional enzyme activity, comparison between Slc1p with Slc4p, single mutants of Slc1p and Slc4p generated, double mutant of Slc1p and Slc4p lethal, same glycerophospholipid profiles but different lipid profiles between Slc1p and Slc4p, both enzymes able to use endogenous lysoglycerophospholipids as substrates, substrate specificity between Slc1p and Slc4p different or access to different lysoglycerophospholipid substrates because of a different subcellular location, Slc1p and Slc4p are both active as acyltransferases and are also involved in fatty acid exchange at the sn-2-position of mature glycerophospholipids; microsomal enzyme activity not completely lost by deletion of Slc1p, evidence for additional enzyme activity, SLC4 gene encodes second enzmye indicated by lethality of double mutants of Slc1p and Slc4p, same glycerophospholipid profile but different lipid profiles between Slc1p and Slc4p, different substrate specificity between Slc1p and Slc4p or access to different lysoglycerophospholipid substrates because of a different subcellular location
additional information
-
absence of transmembrane domains, presence of hydrolase/acyltransferase domain with a distinct lipid-binding motif and a lysophospholipase domain, reduced phosphatidic acid in mutants suggests a role in phosphatidic acid biosynthesis, overexpression shows increase in phosphatidic acid and other phospholipids on organic solvent exposure, recombinant protein acylates lysophosphatidic acid, substrate preference analyzed, nearly no activity with lysophospholipids such as lysophosphatidylcholine (LPC), lysophosphatidylethanolamine (LPE), and lysophosphatidylserine (LPS)
additional information
Q8NF37
overexpression shows 1-acyl-sn-glycero-3-phosphate acyltransferase activity but no lysophosphatidylcholine acyltransferase activity, acyl-CoA specificity and specificity of lysophosphatidic acids (LPAs) listed, substitution of asparagine with proline in the NHX4D motif and arginine with cysteine in the EGTR motif, mutant retains enzyme activity, model proposed in which a hydrophobic pocket in 1-acyl-sn-glycero-3-phosphate acyltransferase 9 accommodates fatty acyl chains of both substrates in proper orientation, whereas the HX4D motif participates in catalysis
additional information
C4B4E7
substrate specificity, overview
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7 - 8.5
Q9DBL9
;
7.4
-
assay at
7.4
-
assay at
7.4
-
assay at
7.4
-
assay at
7.4
-
assay at
7.4
-
assay at
7.5 - 8
-
-
7.5
-
assay at
7.5
P33333, Q08548
activity assay at; activity assay at
7.5
O22975
assay at
8 - 10
-
broad
8 - 9.5
-
broad
8
-
assay at
8
Q9XFW4
assay at
9 - 11
-
broad, assay at pH 10
9
Q59601
assay at
9
Q59618
assay at
9
-
assay at
9
-
assay at
additional information
-
pH profiles of the platelet and liver enzyme systems
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
6.6 - 8.6
-
about half-maximal activity at pH 6.6 and 8.6
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
22
-
assay at
22
-
assay at room temperature
23 - 26
-
assay at
25
-
assay at
25
-
assay at
30
-
assay at
30
-
assay at
30
Q59601
assay at
30
Q59618
assay at
30
-
assay at
30
-
assay at
30
-
assay at
30
-
activity assay at
30
-
activity assay at
30
O22975
assay at
30
Q9XFW4
assay at
35
-
assay at
37
-
assay at
37
C4B4E7
assay at
37
-
assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
O15120
isozyme AGPAT2
Manually annotated by BRENDA team
C4B4E7
low expression level
Manually annotated by BRENDA team
Q8NF37
expressed in
Manually annotated by BRENDA team
-
high expression level of AGPAT10/GPAT3 and AGPAT6/GPAT4
Manually annotated by BRENDA team
Mus musculus C57BL/6J
-
low expression level
-
Manually annotated by BRENDA team
Q643R3
expression of AGPAT7
Manually annotated by BRENDA team
-
cDNA library
Manually annotated by BRENDA team
-
analysis of enzyme mRNA expression, transcript of approximately 2 kb length dominant
Manually annotated by BRENDA team
-
mAGPAT1, mAGPAT2, mAGPAT3, mAGPAT2 is undetectable, mAGPAT4 is expressed at high levels, mAGPAT5 expressed at high levels
Manually annotated by BRENDA team
Q8GXU8, Q8L4Y2, Q8LG50, Q9LHN4, Q9SYC8
-
Manually annotated by BRENDA team
-
whole, analysis of enzyme mRNA expression, only the transcript of approximately 2 kb length is present. In 7-day embryos both bands of 1.3 and 2 kb are present
Manually annotated by BRENDA team
-
isozyme AGPAT2 and AGPAT5 expression increase in parallel with both an increase in enzyme activity and permeability barrier formation late in rat epidermal development, acute permeability barrier disruption leads to rapidly increased expression of isozymes AGPAT 1, 2, and 3
Manually annotated by BRENDA team
C4B4E7
low expression level
Manually annotated by BRENDA team
Q9XFW4
BAT1.13
Manually annotated by BRENDA team
Q8GXU8, Q8L4Y2, Q8LG50, Q9LHN4, Q9SYC8
the endoplasmic reticulum-located LPAT2 is essential for female but not male gametophyte development in Arabidopsis thaliana
Manually annotated by BRENDA team
-
mAGPAT1, mAGPAT2, mAGPAT3, mAGPAT4 is expressed at low levels, mAGPAT5 expressed at intermediate levels
Manually annotated by BRENDA team
-
analysis of enzyme mRNA expression, transcript of approximately 2 kb length dominant
Manually annotated by BRENDA team
C4B4E7
low expression level
Manually annotated by BRENDA team
-
mAGPAT1, mAGPAT2, mAGPAT3, mAGPAT4 barely detectable, mAGPAT5 expressed at high levels, mRNA expression of cardiac mAGPAT3 is regulated by activation of peroxisome-proliferator-activated receptors
Manually annotated by BRENDA team
Mus musculus C57BL/6J
-
low expression level
-
Manually annotated by BRENDA team
Q643R3
expression of AGPAT7
Manually annotated by BRENDA team
Q9NRZ7, Q9NUQ2
high expression
Manually annotated by BRENDA team
-
analysis of enzyme mRNA expression, transcript of approximately 2 kb length dominant
Manually annotated by BRENDA team
C4B4E7
low expression level
Manually annotated by BRENDA team
-
mAGPAT1, mAGPAT2, mAGPAT3, mAGPAT4 is expressed at low levels, mAGPAT5 expressed at intermediate levels
Manually annotated by BRENDA team
Mus musculus C57BL/6J
-
low expression level
-
Manually annotated by BRENDA team
Q9XFW4
BAT1.13
Manually annotated by BRENDA team
Q8NF37
expressed in
Manually annotated by BRENDA team
-
analysis of enzyme mRNA expression
Manually annotated by BRENDA team
-
mAGPAT1, mAGPAT2, mAGPAT3, mAGPAT4 barelry detectable, mAGPAT5 expressed at low levels
Manually annotated by BRENDA team
-
analysis of enzyme mRNA expression, transcript of approximately 2 kb length dominant
Manually annotated by BRENDA team
-
mAGPAT1, mAGPAT3, mAGPAT2 is undetectable, mAGPAT4 expressed at low levels, mAGPAT5 expressed at low levels
Manually annotated by BRENDA team
Q643R3
expression of AGPAT7
Manually annotated by BRENDA team
Q8NF37
high expression in
Manually annotated by BRENDA team
-
high expression level of AGPAT6/GPAT4
Manually annotated by BRENDA team
Q9NRZ7, Q9NUQ2
high expression
Manually annotated by BRENDA team
Q643R3
expression of AGPAT7
Manually annotated by BRENDA team
-
very high expression level of AGPAT6/GPAT4
Manually annotated by BRENDA team
Q8NF37
expressed in
Manually annotated by BRENDA team
Q8GXU8, Q8L4Y2, Q8LG50, Q9LHN4, Q9SYC8
LPAT2 predominantly
Manually annotated by BRENDA team
Q8GXU8, Q8L4Y2, Q8LG50, Q9LHN4, Q9SYC8
-
Manually annotated by BRENDA team
Q9XFW4
BAT1.13, expression at the tip of primary and secondary roots and in mesophyll/cortex tissue behind the elongation zone and in the vascular tissue
Manually annotated by BRENDA team
-
developmenting, maximal activity at 8-14 days after flowering during rapid lipid accumulation, overview
Manually annotated by BRENDA team
Q9XFW4
developing, BAT1.5 , and BAT1.13
Manually annotated by BRENDA team
-
etiolated
Manually annotated by BRENDA team
Q9XFW4
young, BAT1.5 , and BAT1.13
Manually annotated by BRENDA team
-
analysis of enzyme mRNA expression, transcript of approximately 2 kb length dominant
Manually annotated by BRENDA team
-
mAGPAT1, mAGPAT2, mAGPAT, mAGPAT4 expressed at intermediate levels, mAGPAT5 expressed at high levels
Manually annotated by BRENDA team
Q643R3
expression of AGPAT7
Manually annotated by BRENDA team
-
analysis of enzyme mRNA expression, transcript of approximately 1.3 kb length dominant
Manually annotated by BRENDA team
-
mAGPAT1, mAGPAT3, mAGPAT2 is undetectable, mAGPAT4 is expressed at low levels, mAGPAT5 expressed at low levels
Manually annotated by BRENDA team
Q8NF37
high expression in
Manually annotated by BRENDA team
-
high expression level of AGPAT6/GPAT4
Manually annotated by BRENDA team
Q9XFW4
BAT1.13
Manually annotated by BRENDA team
Q643R3
expression of AGPAT7
Manually annotated by BRENDA team
Q9NRZ7, Q9NUQ2
high expression
Manually annotated by BRENDA team
-
analysis of enzyme mRNA expression, transcript of approximately 2 kb length dominant
Manually annotated by BRENDA team
Q643R3
expression of AGPAT7
Manually annotated by BRENDA team
-
high expression level of AGPAT10/GPAT3 and AGPAT6/GPAT4
Manually annotated by BRENDA team
C4B4E7
high expression level, expression of the enzyme in the testis increases significantly in an age-dependent manner
Manually annotated by BRENDA team
Mus musculus C57BL/6J
-
high expression level, expression of the enzyme in the testis increases significantly in an age-dependent manner
-
Manually annotated by BRENDA team
Q643R3
expression of AGPAT7
Manually annotated by BRENDA team
C4B4E7
testicular cell line
Manually annotated by BRENDA team
Q643R3
expression of AGPAT7
Manually annotated by BRENDA team
-
physiological significance analyzed in white adipose tissue of mice, soluble lysophosphatidic acid acyltransferase activity, existence of an alternate cytosolic phosphatidic acid biosynthetic pathway
Manually annotated by BRENDA team
additional information
-
the BAT2 gene is expressed in a ubiquitous manner in all tissues, including nonphotosynthetic tissues where plastids are present
Manually annotated by BRENDA team
additional information
Q8GXU8, Q8L4Y2, Q8LG50, Q9LHN4, Q9SYC8
LPAT2 is expressed ubiquitously in diverse tissues, tissue-specific expression levels, overview
Manually annotated by BRENDA team
additional information
Q643R3
quantitative tissue-specific expression analysis of AGPAT7
Manually annotated by BRENDA team
additional information
-
tissue-specific expression analysis of AGPAT8
Manually annotated by BRENDA team
additional information
Q9XFW4
differences in the expression of the two genes emcoding the two microsomal LPAAT isozymes, one is detected in all rapeseed tissues and during silique and seed development, whereas the expression of the second gene is restricted predominantly to siliques and developing seeds
Manually annotated by BRENDA team
additional information
-
tissue distribution of AGPAT10/GPAT3 and AGPAT6/GPAT4 expression, overview
Manually annotated by BRENDA team
additional information
C4B4E7
tissue distribution of LPAAT3 expression, overview
Manually annotated by BRENDA team
additional information
Q9NRZ7, Q9NUQ2
isoform AGPAT3 appears to be ubiquitously expressed across the tissues examined. Comparison of isoforms AGPAT3, AGPAT4, AGPAT5 expression in different tissues
Manually annotated by BRENDA team
additional information
Mus musculus C57BL/6J
-
tissue distribution of LPAAT3 expression, overview
-
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
-
mature protein of 32000 Da processed from the precursor protein of 38000 Da
Manually annotated by BRENDA team
O35083
isoform AGPAT1 localizes to cytoskeletal components, particularly to areas of actin polymerization in myoblasts
Manually annotated by BRENDA team
Q8GXU8, Q8L4Y2, Q8LG50, Q9LHN4, Q9SYC8
LPAT1
Manually annotated by BRENDA team
Q8GXU8, Q8L4Y2, Q8LG50, Q9LHN4, Q9SYC8
LPAT3
Manually annotated by BRENDA team
Q8GXU8, Q8L4Y2, Q8LG50, Q9LHN4, Q9SYC8
LPAT4
Manually annotated by BRENDA team
Q8GXU8, Q8L4Y2, Q8LG50, Q9LHN4, Q9SYC8
LPAT5
Manually annotated by BRENDA team
-
white adipose tissue of mice, precise role of cgi-58 protein in the cytoplasm unknown
Manually annotated by BRENDA team
O15120
AGPAT2 is an integral membrane protein
Manually annotated by BRENDA team
Q8GXU8, Q8L4Y2, Q8LG50, Q9LHN4, Q9SYC8
LPAT2, co-localizes with calreticulin in the endoplasmic reticulum
Manually annotated by BRENDA team
Q8NF37
same domain as AGPAT1
Manually annotated by BRENDA team
-
both isoforms AGPAT1 and AGPAT2
Manually annotated by BRENDA team
P33333, Q08548
enzyme contains the conserved His residue close to a short stretch of hydrophilic amino acids, which is intercalated between two potential transmembrane helices. The active site histidine resides in the lumen of the endoplasmic reticulum
Manually annotated by BRENDA team
P33333, Q08548
the most conserved motif containing the presumed active site histidine of isoform Slc1 is oriented in the the membrane towards the lumen, whereas other conserved motifs are cytosolic.embrane to the endereas other conserved motifs are cytosolic
Manually annotated by BRENDA team
Mus musculus C57BL/6J
-
-
-
Manually annotated by BRENDA team
-
white adipose tissue of mice
-
Manually annotated by BRENDA team
Q06508
and endoplasmic reticulum
-
Manually annotated by BRENDA team
-
more than 60% of total activity associated with membranes in white adipose tissue
Manually annotated by BRENDA team
Euglena sp.
-
-
-
Manually annotated by BRENDA team
-
predominantly located in endoplasmic reticulum
-
Manually annotated by BRENDA team
-
not rat liver peroxisomes, intrinsic membrane protein, cytoplasmic surface
-
Manually annotated by BRENDA team
Q9XFW4
LPAAT is located in the cytoplasmic endomembrane compartment, two microsomal LPAAT isozymes are identified. Diversity of LPAAT microsomal isozymes in rapeseed, overview
-
Manually annotated by BRENDA team
Saccharomyces cerevisiae OC-2
-
-
-
-
Manually annotated by BRENDA team
-
the presence of the enzyme in the mitochondrial fraction cannot be explained by the contamination of microsomes in this fraction
Manually annotated by BRENDA team
Saccharomyces cerevisiae OC-2
-
the presence of the enzyme in the mitochondrial fraction cannot be explained by the contamination of microsomes in this fraction
-
Manually annotated by BRENDA team
additional information
C4B4E7
subcellular distribution of LPAAT3, overview
-
Manually annotated by BRENDA team
additional information
Mus musculus C57BL/6J
-
subcellular distribution of LPAAT3, overview
-
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
29000
-
apparent, gel filtration
487201
32000
-
mature protein, processing of a BAT2 precursor protein, chloroplast-import experiments
487207
32000
-
Western blotting
487210
34800
-
calculated from amino acid sequence
487201
38000
-
predicted, protein encoded by the BAT2 cDNA
487207
44000
-
estimated, high-speed supernatant, gel filtration
487198
61180
P33333, Q08548
partially purified Slc1p, SDS-PAGE, Western Blot
687624
additional information
-
45 kDa protein in Western Blot and mass spectrometry analysis, subcellular fractions of white adipose tissue
687752
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
?
C4B4E7
x * 43300, about, sequence calculation, x * 37000, about, recombinant FLAg-tagged enzyme, SDS-PAGE
?
-
x * 43380, claculated
?
Mus musculus C57BL/6J
-
x * 43300, about, sequence calculation, x * 37000, about, recombinant FLAg-tagged enzyme, SDS-PAGE
-
additional information
Q9DBL9
the CGI-58 carboxyl terminus includes a highly conserved consensus sequence, HXXXXD, required for acyltransferase activity
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
6.5 - 7.5
-
lowering the assay pH from 7.5 to 6.5 results in a loss of approximately 20% of the enzyme activity
487198
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
43
-
t1/2: 62 min
486634
47
Q9DBL9
10 min, 60% loss of activity
705011
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
slow freezing inactivates
-
ethylene glycol, 20% v/v, stabilizes
-
the long time required for fractionation after hypotonic hemolysis probably contributes to the loss of activity
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4C, high-speed supernatant preparation, 50% loss of activity, overnight
-
4C, high-speed supernatant, reactivated with phospholipids, no loss of activity
-
-15C to 4C, particulate preparation, fast-frozen in liquid N2, unstable
-
-15C, less than a day
-
-80C, particulate preparation, fast-frozen in liquid N2, 3 months
-
-20C, particulate preparation, several days
-
4C, soluble preparation, at least 1 day
-
-20C, microsomal preparation, at least 3 weeks
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant His-tagged At4g24160 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
O22975
identification of enzyme using solubilization with CHAPS and column chromatography on Red 120 agarose, Bio-Rad HA HT, 12:0-CoA Sepharose 4B, and Superose 12
-
partial, separated from EC 2.3.1.15 using Triton X-100, the enzyme is not solubilized by Triton X-100 or cholate
-
gel filtration and SDS-PAGE, recombinant protein, mutant and wild-type
-
gel filtration, wild-type and mutant protein
-
recombinant enzyme partially from CHO-K1 cell microsomes
O15120
native enzyme partially by microsome preparation
-
partial
-
partial; using solubilization with CHAPS at high ionic strength, and anion-exchange chromatography on Mono Q column
-
partial; using solubilization with Triton X-100 and gel filtration, separation from EC 2.3.1.23
-
gel filtration and SDS-PAGE, recombinant protein
-
partially purified, gel filtration; partially purified, gel filtration
P33333, Q08548
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
-
gene At4g24160, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3)
O22975
gene LPAT1, genetic structure, phylogenetic tree; gene LPAT2, genetic structure, LPAT2-cDNA driven by an LPAT2 promoter functionally complements insertion mutant lpat2 in transformed heterozygous mutants to produce the lpat2/lpat2 genotype, overview, phylogenetic tree, functional expression in Saccharomyces cerevisiae, genotyping; gene LPAT3, genetic structure, phylogenetic tree, functional expression in Saccharomyces cerevisiae, genotyping; gene LPAT4, genetic structure, phylogenetic tree, expression in Saccharomyces cerevisiae results in an inactive enzyme; gene LPAT5, genetic structure, phylogenetic tree, expression in Saccharomyces cerevisiae results in an inactive enzyme
Q8GXU8, Q8L4Y2, Q8LG50, Q9LHN4, Q9SYC8
genes BAT1.13 and BAT1.5, cloning of two cDNAs encoding microsomal isozymes from an immature embryo library, sequence comparisons, functional expression of the two rapeseed microsomal LPAAT isozymes in Arabidopsis thaliana, using Agrobacterium tumefaciens strain C58C1 transfection, leading to enhanced oil content and seed weight, tissue localization study, overview. Complementationof a thermosensitive Escherichia coli strain JC201 by expression of Bat1.5 and BAT1.13, overview
Q9XFW4
isolation of a cDNA encoding an enzyme by functional complementation of the Escherichia coli mutant plsC with an immature embryo cDNA library of oilseed rape. Transformation of the acyltransferase-deficient Escherichia coli strain JC201 with the cDNA sequence BAT2 alleviates the temperature-sensitive phenotype of the plsC mutant and confers a palmitoyl-CoA-preferring enzyme activity to membrane fractions. Mapping of the BAT2 genes and sequence analysis of the BAT2 cDNA clone
-
transformation of a modified plasmid into Escherichia coli JC201 enzyme-deficient strain and transformation of the plasmid DNA containing the cDNA BAT2 encoding the plastidial enzyme into the Escherichia coli XL-1 red mutator strain
Q9XFW4
amino acid sequence data used to isolate a full-length clone from a coconut endosperm cDNA library, expression of the cDNA in Escherichia coli
-
expression of a cDNA encoding protein with enzyme activity in transgenic plants. Expression in canola results in incorporation of laurate into the sn-2 position of triacylglycerol
-
isolation of the JC201-plsC gene fragment containing the entire sequence of the enzyme. Complementation of the JC201 phenotype: transformation of JC201 cells with pplSC6 or pplsC6.3. Transformation of XL1-Blue cells with the control plasmid pplsC6.3, and the experimental plasmids pplsC6.3A or pplsC6.3L
-
modification of the gene of Escherichia coli which encodes the enzyme by inserting an endoplasmic reticulum retrieval signal to its 3'end and introduccion into rapeseed plants under the control of a napin promotor
-
AGPAT10/GPAT3, expression in CHO cells endoplasmic reticulum, and as AGPAT-active enzyme in HEK-293 cells via transfection with an adenoviral vector, overexpression of the AGPAT-active enzyme in Huh-7 cells depleted of endogenous AGPAT activity by shRNA-AGPAT2-lentivirus
-
expressed in Baculovirus-Sf9 insect cells, pESC-URA yeast expression vector with a c-myc-epitope, transformation into Escherichia coli after site-directed mutagenesis
-
expressed in Escherichia coli, plasmids pCR2.1, pDrive, pcDNA 3.1(C)neo, construction of the wild-type fusion protein with GFP, overexpression in CHO-cells, coexpression in CHO-cells with AGPAT1
Q8NF37
expression in Chineses hamster ovary cells
-
expression in CHO cell, HeLa cell and HEK-293 cell
Q7L5N7
expression in HEK-293 cell; expression in HEK-293 cell
Q9NRZ7, Q9NUQ2
expression in insect and mammalian cell lines. The enzyme gene is located in the MHC class III region
-
expression in osteosarcoma cell
-
expression via recombinant adenoviruses in Agpat2 -/- mice; expression via recombinant adenoviruses in Agpat2 -/- mice
-
gene AGPAT2, expression of C-terminally HA-tagged AGPAT2 in CHO-K1 cells
O15120
gene AGPAT7, DNA and amino acid sequence determination and analysis, chromosomal mapping to 15q14, expression analysis
Q643R3
gene AGPAT8, DNA and amino acid sequence determination and analysis, localization on chromosome 2p23.1, genetic structure, expression analysis, stable expression of wild-type and mutant enzymes in CHO cells
-
overexpressed in Escherichia coli BL21 (DE3), pCMV-SPORT6 vector, overexpressed in Saccharomyces cerevisiae, pYES2 vector
-
stable functional expression of GFP-tagged LPAAT3 in HeLa cells and in Rattus norvegicus hepatocytes with localization in the Golgi membranes
-
isolation of a potential cDNA for the enzyme by heterologous screening with the maize pMAT1 clone, clone pLAT1; isolation of a potential cDNA for the enzyme by in vivo complementation of the Escherichia coli mutant JC201, clone pLAT2 is an enzyme clone
Q40119, Q42870
transformation of plants of spring high-erocic acid rape, Hero, with a construct carrying the Limnanthes douglasii enzyme, LAT2, under control of the napin promoter. Study of the correlation between 22:1 accumulation at sn-2, production of trierucin and the expression of the transgene as detected by Western blot
-
expression in C2C12 cell
O35083
expression in Escherichia coli JC201, a mutant strain deficient in the enzyme activity
-
expression in Escherichia coli; expression of active His6-tagged CGI-58 in Escherichia coli
Q9DBL9
expression in JC201 Escherichia coli cells
-
gene AGPAT8, DNA and amino acid sequence determination and analysis, localization on chromosome 17, genetic structure, expression analysis
-
LPAAT3, phylogenetic analysis, expression of the FLAG-tagged enzyme in CHO-K1 cells
C4B4E7
isolation and characterization of a gonococcal mutant
Q59601
isolation and characterization of a meningococcal mutant, cloning of the meningococcal mutant gene
Q59618
expressed in Escherichia coli strain HB101, transformed into Saccharomyces cerevisiae, strains, plasmids and sources of parent vectors listed; expressed in Escherichia coli strain HB101, transformed into Saccharomyces cerevisiae, strains, plasmids and sources of parent vectors listed
P33333, Q08548
expressed in Escherichia coli, overexpression in Saccharomyces cerevisiae, expression plasmid pPS189
-
expression in Escherichia coli
Q06508
gene plsC, subcloning in Escherichia coli strain DH5alpha
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
expression of isoform AGPAT11 mRNA is significantly upregulated in human breast, cervical, and colorectal cancer tissues
Q7L5N7
expression of the enzyme in the testis increases significantly in an age-dependent manner, which is regulated by induction through beta-estradiol, in TM4 cells
C4B4E7
isoform LPAAT3 is induced during germ cell maturation. Differentiation of mouse GC-2spd(ts) spermatocytes into spermatides up-regulates isoform LPAAT3 mRNA, increases the amount of polyunsaturated phospholipids, and shifts the specificity for the incorporation of docosahexaenoic acid toward phosphatidylcholine and phosphatidylethanolamine
-
expression of the enzyme in the testis increases significantly in an age-dependent manner, which is regulated by induction through beta-estradiol, in TM4 cells
Mus musculus C57BL/6J
-
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
C247Y
Q9XFW4
loss of the ability of the plastidial enzyme to restore growth to JC201, suggests that C247 is essential for activity
D199A
Q9XFW4
the mutant fails to grow beyond 0.04 D600 after transfer to the non-permissive temperature. D199 is essential for the ability of the plastidial enzyme to complement the defective acyltransferase activity of JC201
E266A
Q9XFW4
the plastidial enzyme containing this mutation is unable to complement the temperature-sensitive phenotype of JC201, indicating an essential role for this acidic residue
G140S
Q9XFW4
loss of the ability of the plastidial enzyme to restore growth to JC201, suggests that G140 is essential for activity
H194A
Q9XFW4
the mutant fails to grow beyond 0.04 D600 after transfer to the non-permissive temperature. H194 is essential for the ability of the plastidial enzyme to complement the defective acyltransferase activity of JC201
K84Q
Q9XFW4
greatly impaired growth rate, possibly indicating an importance of the N-terminus of the mature protein in determining enzyme activity
L203P
Q9XFW4
loss of the ability of the plastidial enzyme to restore growth to JC201, suggests that L203 is essential for activity
P187L
Q9XFW4
loss of the ability of the plastidial enzyme to restore growth to JC201, suggests that P187 is essential for activity
T122A
-
the change increases the substrate specificity in vitro for oleoyl-CoA and linoleoyl-CoA
T122L
-
the change increases the substrate specificity in vitro for lignoceroyl-CoA
140delF
-
34.8% of wild-type activity
221delGT
-
5% of wild-type activity
252delMRT
-
3.2% of wild-type activity
A239V
-
90% of wild-type activity
D168R
-
site-directed mutagenesis, the mutant shows slightly increased activity compared to the wild-type AGPAT8
D180fsX251
-
7.7% of wild-type activity
E260K
-
site-directed mutagenesis, mutation found in Chanarin-Dorfman syndrome patients, mutation without significant effect on acyltransferase activity
G136R
-
40.3% of wild-type activity
I208A
-
mutant of human AGPAT1
P207L
-
mutant of human AGPAT1
Q130P
-
site-directed mutagenesis, mutation found in Chanarin-Dorfman syndrome patients, mutation without significant effect on acyltransferase activity
R149K
-
mutant of human AGPAT1
R68X
-
14.2% of wild-type activity
T180S
-
mutant of human AGPAT1
additional information
Q8GXU8, Q8L4Y2, Q8LG50, Q9LHN4, Q9SYC8
identification of a null allele lpat2 having a T-DNA inserted into LPAT2, the heterozygous mutant LPAT2/lpat2 has a minimal altered vegetative phenotype but produces shorter siliques that contain normal seeds and remnants of aborted ovules in a 1:1 ratio, overview, lpat2 causes lethality in the female gametophyte but not the male gametophyte, which has the redundant LPAT3, that complements, phenotypes, overview
additional information
Q8GXU8, Q8L4Y2, Q8LG50, Q9LHN4, Q9SYC8
LPAT3-cDNA functionally complements the lpat2 LPAT2 mutant strain in part in heterozygous LPAT2/lpat2 and homozygous lpat2/lpat2 mutants, overview
R92G
Q9XFW4
greatly impaired growth rate, possibly indicating an importance of the N-terminus of the mature protein in determining enzyme activity
additional information
-
adenine-440 is responsible for the temperature sensitive lesion in the enzyme activity of JC201. Conversion of the mutant adenine-440 back to the native guanine-440 nucleotide restores the enzyme activity at the non-permissive temperature of 42C
L228P
-
23.7% of wild-type activity
additional information
O15120
small interference RNA-mediated knockdown of AGPAT2 expression in OP-9 cells prevents appropriate early induction of C/EBPbeta and PPARgamma, key transcriptional activators of the adipogenic program, and delays expression of multiple adipocyte-related genes
additional information
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generation of stable Huh-7 cells with a knockout for AGPAT2 activity by shRNA-AGPAT2-lentivirus
additional information
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overexpression of LPAAT3 in HeLa cells significantly inhibits the formation of Golgi membrane tubules in vivo and in vitro. Anterograde and retrograde protein trafficking is slower in cells overexpressing LPAAT3 and accelerated in cells with expression reduced by siRNA, LPAAT3 knockdown leads to Golgi fragmentation, overview
additional information
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recombinant expression of murine CGI-58 in fibroblasts from humans with Chanarin-Dorfman syndrome increases the incorporation of radiolabeled fatty acids released from the lipolysis of stored triacylglycerols into phospholipids
V167fsX228
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14.9% of wild-type activity
additional information
Q9DBL9
recombinant expression of murine CGI-58 in fibroblasts from humans with Chanarin-Dorfman syndrome increases the incorporation of radiolabeled fatty acids released from the lipolysis of stored triacylglycerols into phospholipids
additional information
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construction of a PlsC knockout mutant, which shows 25fold decreased phosphatidylglycerols 18:1/16:0 ratio, phospholipid composition of mutant relative to wild-type cells, overview
Renatured/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
reassociation of the solubilized enzyme with soybean phospholipids
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by mixing 1 vol. of the fraction containing the enzyme with 4 vol. of sonicated phosphatidylcholine/phosphatidylglycerol or total E. coli lipid vesicles
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
medicine
Q7L5N7
expression of isoform AGPAT11 mRNA is signifi cantly upregulated in human breast, cervical, and colorectal cancer tissues
medicine
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expression of isoform LPAATbeta is readily detected in 8 of 10 analyzed human osteosarcoma lines. Exogenous expression of LPAATbeta promotes osteosarcoma cell proliferation and migration, while silencing LPAATbeta expression inhibits these cellular characteristics. Exogenous expression of LPAATbeta effectively promotes tumor growth, while knockdown of LPAATbeta expression inhibits tumor growth in an orthotopic xenograft model of human osteosarcoma
medicine
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the role of isoforms AGPAT1 or AGPAT2 in liver lipogenesis is minimal and accumulation of liver fat is primarily a consequence of insulin resistance and loss of adipose tissue in Agpat2 -/- mice
medicine
Q9DBL9
expression of isoform CGI-58 in fibroblasts from humans with Chanarin-Dorfman increases the incorporation of fatty acids released from the lipolysis of stored triacylglycerols into phospholipids