Information on EC 2.3.1.51 - 1-acylglycerol-3-phosphate O-acyltransferase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
2.3.1.51
-
RECOMMENDED NAME
GeneOntology No.
1-acylglycerol-3-phosphate O-acyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate = CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acyl group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Biosynthesis of secondary metabolites
-
-
CDP-diacylglycerol biosynthesis
-
-
CDP-diacylglycerol biosynthesis I
-
-
CDP-diacylglycerol biosynthesis II
-
-
CDP-diacylglycerol biosynthesis III
-
-
diacylglycerol and triacylglycerol biosynthesis
-
-
Glycerolipid metabolism
-
-
Glycerophospholipid metabolism
-
-
Metabolic pathways
-
-
oleate biosynthesis III (cyanobacteria)
-
-
palmitoleate biosynthesis III (cyanobacteria)
-
-
phospholipid remodeling (phosphatidate, yeast)
-
-
plasmalogen biosynthesis
-
-
stigma estolide biosynthesis
-
-
superpathway of phosphatidate biosynthesis (yeast)
-
-
SYSTEMATIC NAME
IUBMB Comments
acyl-CoA:1-acyl-sn-glycerol-3-phosphate 2-O-acyltransferase
Acyl-[acyl-carrier protein] can also act as an acyl donor. The animal enzyme is specific for the transfer of unsaturated fatty acyl groups.
CAS REGISTRY NUMBER
COMMENTARY hide
51901-16-7
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Jersey cow
-
-
Manually annotated by BRENDA team
coconut
-
-
Manually annotated by BRENDA team
Euglena sp.
-
-
-
Manually annotated by BRENDA team
cvs. AC Emerson and Vimy
-
-
Manually annotated by BRENDA team
Mus musculus C57BL/6J
C57BL/6J mice
C4B4E7
UniProt
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
OC-2
-
-
Manually annotated by BRENDA team
gene plsC or SMc00714, strain RmP110
-
-
Manually annotated by BRENDA team
potato
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
-
mutations in human CGI-58/ABHD5 cause Chanarin-Dorfman syndrome, characterized by excessive storage of triacylglycerol in tissues
metabolism
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1-oleoyl-sn-glycerol-3-phosphate + oleoyl-CoA
CoA + 1,2-oleoyl-sn-glycerol-3-phosphate
show the reaction diagram
-
-
-
-
?
1-oleoyl-sn-glycerol-3-phosphate + palmitoyl-CoA
CoA + 1-oleoyl-2-palmitoyl-sn-glycerol-3-phosphate
show the reaction diagram
-
-
-
-
?
acyl-ACP + 1-acyl-sn-glycerol 3-phosphate
ACP + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
acyl-CoA + 1-arachidonoyl-sn-glycerol 3-phosphate
CoA + 1-arachidonoyl-2-acyl-sn-glycerol 3-phosphate
show the reaction diagram
acyl-CoA + 1-lauroyl-sn-glycerol 3-phosphate
CoA + 1-lauroyl-2-acyl-sn-glycerol 3-phosphate
show the reaction diagram
-
assayed using coconut cDNA encoding a 299-amino acid protein with enzyme activity expressed in canola seeds
-
?
acyl-CoA + 1-O-alkyl-sn-glycerol 3-phosphate
CoA + ?
show the reaction diagram
-
the activity is approximately half of that toward 1-acyl-sn-glycerol 3-phosphate
-
?
acyl-CoA + 1-oleoyl-sn-glycerol 3-phosphate
CoA + 2-acyl-1-oleoyl-sn-glycerol 3-phosphate
show the reaction diagram
acyl-CoA + 1-palmitoyl-sn-glycerol 3-phosphate
CoA + 1-palmitoyl-2-acyl-sn-glycerol 3-phosphate
show the reaction diagram
acyl-CoA + 1-stearoyl-sn-glycerol 3-phosphate
CoA + 1-stearoyl-2-acyl-sn-glycerol 3-phosphate
show the reaction diagram
acyl-CoA + 2-acyl-sn-glycerol 3-phosphate
CoA + ?
show the reaction diagram
acyl-CoA + linoleoyl-sn-glycerol 3-phosphate
CoA + ?
show the reaction diagram
acyl-CoA + myristoyl-sn-glycerol 3-phosphate
CoA + ?
show the reaction diagram
acyl-[acyl-carrier protein] + 1-acyl-sn-glycerol 3-phosphate
ACP + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
arachidonoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-arachidonoyl-sn-glycerol 3-phosphate
show the reaction diagram
arachidonoyl-CoA + 1-arachidonoyl-2-lyso-sn-phosphatidylcholine
CoA + 1,2-diarachidonoyl-sn-phosphatidylcholine
show the reaction diagram
-
-
-
?
arachidonoyl-CoA + 1-arachidonoyl-sn-glycerol 3-phosphate
CoA + 1,2-diarachidonoyl-sn-glycerol 3-phosphate
show the reaction diagram
slight preference for 1-oleoyl lysophosphatidic acid over 1-palmitoyl, 1-stearoyl, or 1-arachidonoyl lysophosphatidic acid
-
-
?
arachidonoyl-CoA + 1-octadecenyl-2-lysophosphatidylinositol
CoA + 1-octadecenyl-2-arachidonoylphosphatidylinositol
show the reaction diagram
C4B4E7
-
-
-
?
arachidonoyl-CoA + 1-oleoyl-2-lysophosphatidylcholine
CoA + 1-oleoyl-2-arachidonoylphosphatidylcholine
show the reaction diagram
C4B4E7
-
-
-
?
arachidonoyl-CoA + 1-oleoyl-glycerol-3-phosphate
1-oleoyl-2-arachidonoyl-glycerol-3-phosphate + CoA
show the reaction diagram
-
-
-
-
?
arachidonoyl-CoA + 1-oleoyl-sn-glycerol 3-phosphate
CoA + 1-oleoyl-2-arachidonoyl-sn-glycerol 3-phosphate
show the reaction diagram
arachidonoyl-CoA + 1-oleoyl-sn-lysophosphatidylcholine
CoA + 1-oleoyl-2-arachidonoylphosphatidylcholine
show the reaction diagram
-
-
-
?
arachidonoyl-CoA + 1-palmitoyl-2-lysophosphatidylinositol
CoA + 1-palmitoyl-2-arachidonoylphosphatidylinositol
show the reaction diagram
C4B4E7
-
-
-
?
arachidonoyl-CoA + 1-palmitoyl-sn-glycerol 3-phosphate
CoA + 1-palmitoyl-2-arachidonoyl-sn-glycerol 3-phosphate
show the reaction diagram
slight preference for 1-oleoyl lysophosphatidic acid over 1-palmitoyl, 1-stearoyl, or 1-arachidonoyl lysophosphatidic acid
-
-
?
arachidonoyl-CoA + 1-palmitoyl-sn-lysophosphatidylcholine
CoA + 1-palmitoyl-2-arachidonoylphosphatidylcholine
show the reaction diagram
-
-
-
?
arachidonoyl-CoA + 1-stearoyl-2-lysophosphatidic acid
CoA + 1-stearoyl-2-arachidonoyl-sn-glycerol 3-phosphate
show the reaction diagram
C4B4E7
-
-
-
?
arachidonoyl-CoA + 1-stearoyl-sn-glycerol 3-phosphate
CoA + 1-oleoyl-2-stearoyl-sn-glycerol 3-phosphate
show the reaction diagram
slight preference for 1-oleoyl lysophosphatidic acid over 1-palmitoyl, 1-stearoyl, or 1-arachidonoyl lysophosphatidic acid
-
-
?
arachidonoyl-CoA + 1-stearoyl-sn-lysophosphatidylcholine
CoA + 1-stearoyl-2-arachidonoyl-sn-glycerol 3-phosphocholine
show the reaction diagram
-
-
-
?
arachidoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-arachidoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
?
behenoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-behenoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
capryl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-capryl-sn-glycerol 3-phosphate
show the reaction diagram
cis-11-eicosenoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-cis-11 eicosenoyl-sn-glycerol 3-phosphate
show the reaction diagram
docosahexaenoyl-CoA + 1-oleoyl-sn-glycerol 3-phosphate
CoA + 1-oleoyl-2-docosahexaenoyl-sn-glycerol 3-phosphate
show the reaction diagram
C4B4E7
best substrates
-
-
?
eicosatetraenoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-eicosatetraenoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
erucoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-erucoyl-sn-glycerol 3-phosphate
show the reaction diagram
erucoyl-CoA + 1-erucoyl-sn-glycerol-3-phosphate
CoA + 1,2-dierucoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
?
gondoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-gondoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
gondoyl-CoA is utilized less efficiently for the reaction than oleoyl-CoA
-
?
heptadecanoyl-CoA + 1-oleoyl-sn-glycerol 3-phosphate
CoA + 1-oleoyl-2-heptadecanoyl-sn-glycerol 3-phosphate
show the reaction diagram
lauroyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-lauroyl-sn-glycerol 3-phosphate
show the reaction diagram
lignoceroyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-lignoceroyl-sn-glycerol 3-phosphate
show the reaction diagram
linolenoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-linolenoyl-sn-glycerol 3-phosphate
show the reaction diagram
linoleoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-linoleoyl-sn-glycerol 3-phosphate
show the reaction diagram
linoleoyl-CoA + 1-oleoyl-sn-glycerol 3-phosphate
CoA + 1-oleoyl-2-linoleoyl-sn-glycerol 3-phosphate
show the reaction diagram
myristoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-myristoyl-sn-glycerol 3-phosphate
show the reaction diagram
myristoyl-CoA + 1-oleoyl-sn-glycerol 3-phosphate
CoA + 1-oleoyl-2-myristoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
about 25% of the activity with oleoyl-CoA by AGPAT10/GPAT3
-
-
?
oleoyl-ACP + sn-1-oleoylglycerol 3-phosphate
ACP + sn-1,2-dioleoylglycerol 3-phosphate
show the reaction diagram
-
microsomal fractions from developing, transgenic seeds but not of untransformed plants
-
?
oleoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-oleoyl-sn-glycerol 3-phosphate
show the reaction diagram
oleoyl-CoA + 1-arachidonoyl-sn-lysophosphatidylcholine
CoA + 1-arachidonoyl-2-oleoyl-sn-glycero-3-phosphocholine
show the reaction diagram
-
-
-
?
oleoyl-CoA + 1-oleoyl-2-lysophosphatidylcholine
CoA + 1,2-dioleoyl-sn-glycero-3-phosphocholine
show the reaction diagram
oleoyl-CoA + 1-oleoyl-2-lysophosphatidylethanolamine
CoA + 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine
show the reaction diagram
-
about 10% of the activity with 1-oleoyl-2-lysophopshatidic acid by AGPAT10/GPAT3
-
-
?
oleoyl-CoA + 1-oleoyl-2-lysophosphatidylglycerol
CoA + 1,2-dioleoyl-sn-glycero-3-phosphate
show the reaction diagram
-
about 10% of the activity with 1-oleoyl-2-lysophopshatidic acid by AGPAT10/GPAT3
-
-
?
oleoyl-CoA + 1-oleoyl-2-lysophosphatidylinositol
CoA + 1,2-dioleoyl-sn-phosphatidylinositol
show the reaction diagram
-
about 10% of the activity with 1-oleoyl-2-lysophopshatidic acid by AGPAT10/GPAT3
-
-
?
oleoyl-CoA + 1-oleoyl-2-lysophosphatidylserine
CoA + 1,2-dioleoyl-sn-glycerol-3-phospho-L-serine
show the reaction diagram
-
about 10% of the activity with 1-oleoyl-2-lysophopshatidic acid by AGPAT10/GPAT3
-
-
?
oleoyl-CoA + 1-oleoyl-sn-glycerol 3-phosphate
CoA + 1,2-dioleoyl-sn-glycerol 3-phosphate
show the reaction diagram
oleoyl-CoA + 1-oleoyl-sn-glycerol-3-phosphate
CoA + 1,2-dioleoyl-sn-glycerol 3-phosphate
show the reaction diagram
oleoyl-CoA + 1-palmitoyl-sn-glycerol 3-phosphate
CoA + 1-palmitoyl-2-oleoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
oleoyl-CoA + 1-palmitoyl-sn-lysophosphatidylcholine
CoA + 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine
show the reaction diagram
-
-
-
?
oleoyl-CoA + 1-stearoyl-sn-lysophosphatidylcholine
CoA + 1-stearoyl-2-oleoyl-sn-glycero-3-phosphocholine
show the reaction diagram
-
-
-
?
oleoyl-CoA + lysophosphatidic acid
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
-
?
palmitoleoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-palmitoleoyl-sn-glycerol 3-phosphate
show the reaction diagram
palmitoyl-ACP + 1-acyl-sn-glycerol 3-phosphate
ACP + 1-acyl-2-palmitoyl-sn-glycerol 3-phosphate
show the reaction diagram
palmitoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-palmitoyl-sn-glycerol 3-phosphate
show the reaction diagram
palmitoyl-CoA + 1-oleoyl-sn-glycerol 3-phosphate
CoA + 1-oleoyl-2-palmitoyl-sn-glycerol 3-phosphate
show the reaction diagram
pentadecanoyl-CoA + 1-oleoyl-sn-glycerol 3-phosphate
CoA + 1-oleoyl-2-pentadecanoyl-sn-glycerol 3-phosphate
show the reaction diagram
stearoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-stearoyl-sn-glycerol 3-phosphate
show the reaction diagram
stearoyl-CoA + 1-oleoyl-sn-glycerol 3-phosphate
CoA + 1-oleoyl-2-stearoyl-sn-glycerol 3-phosphate
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
acyl-[acyl-carrier protein] + 1-acyl-sn-glycerol 3-phosphate
ACP + 1,2-diacyl-sn-glycerol 3-phosphate
show the reaction diagram
arachidonoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
CoA + 1-acyl-2-arachidonoyl-sn-glycerol 3-phosphate
show the reaction diagram
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
no Mg2+-requirement
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1-acyl-sn-glycero 3-phosphate
-
high concentration
1-palmitoyl-sn-glycerol 3-phosphate
-
above 0.05 mM
2-mercaptoethanol
-
above 5 mM
2-[3,5-Di-(t-butyl-4-hydroxyphenyl)thio]hexanate
-
i.e. DH990, weak
Ca2+
-
50% inhibition above 10 mM
CaCl2
1 microM, 60% inhibition. Addition of EDTA restores activity
CI-976
-
inhibits LPAAT and stimulates Golgi tubules
lysophosphatidylcholine
-
inhibits 68% of the enzyme activity at 30 microM
Mn2+
-
50% inhibition above 10 mM
N-ethylmaleimide
Euglena sp.
-
-
NaCl
-
above 200 mM
Phospholipids
-
activity inhibited by high concentrations of
Sodium deoxycholate
-
concentration-dependent inactivation
stearoyl-CoA
-
strongly inhibitory at 0.002 mM
Triton X-100
additional information
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
bovine serum albumin
-
induces T-lymphocyte enzyme activity in the presence of palmitoyl-CoA but not oleoyl-CoA
-
isopropyl-beta-D-thiogalactopyranoside
-
induction of the enzyme gene
additional information
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0053 - 0.125
1-acyl-sn-glycerol 3-phosphate
0.0048 - 0.018
1-oleoyl-sn-glycerol 3-phosphate
0.003
1-palmitoyl-sn-glycerol 3-phosphate
-
with oleoyl-CoA
0.0138
Docosahexaenoyl-CoA
-
pH 7.5, 37°C
0.0027 - 0.0215
oleoyl-CoA
0.0014 - 0.012
palmitoyl-CoA
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
22
oleoyl-CoA
Homo sapiens
-
pH 7.4, 37°C, recombinant AGPAT10/GPAT3
281
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0000108
-
inner mitochondrial membrane
0.0000192
-
mitochondria
0.0002742
-
outer mitochondrial membrane
0.0066
-
-
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5 - 8
-
-
8 - 10
-
broad
8 - 9.5
-
broad
9 - 11
-
broad, assay at pH 10
additional information
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.6 - 8.6
-
about half-maximal activity at pH 6.6 and 8.6
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
23 - 26
-
assay at
35
-
assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
expression of AGPAT7
Manually annotated by BRENDA team
-
isozyme AGPAT2 and AGPAT5 expression increase in parallel with both an increase in enzyme activity and permeability barrier formation late in rat epidermal development, acute permeability barrier disruption leads to rapidly increased expression of isozymes AGPAT 1, 2, and 3
Manually annotated by BRENDA team
C4B4E7
low expression level
Manually annotated by BRENDA team
the endoplasmic reticulum-located LPAT2 is essential for female but not male gametophyte development in Arabidopsis thaliana
Manually annotated by BRENDA team
-
mAGPAT1, mAGPAT2, mAGPAT3, mAGPAT4 is expressed at low levels, mAGPAT5 expressed at intermediate levels
Manually annotated by BRENDA team
expression of AGPAT7
Manually annotated by BRENDA team
-
lactating
Manually annotated by BRENDA team
-
etiolated
Manually annotated by BRENDA team
expression of AGPAT7
Manually annotated by BRENDA team
C4B4E7
testicular cell line
Manually annotated by BRENDA team
expression of AGPAT7
Manually annotated by BRENDA team
-
physiological significance analyzed in white adipose tissue of mice, soluble lysophosphatidic acid acyltransferase activity, existence of an alternate cytosolic phosphatidic acid biosynthetic pathway
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
mature protein of 32000 Da processed from the precursor protein of 38000 Da
Manually annotated by BRENDA team
isoform AGPAT1 localizes to cytoskeletal components, particularly to areas of actin polymerization in myoblasts
Manually annotated by BRENDA team
-
integral enzyme
Manually annotated by BRENDA team
additional information