Literature summary for 2.3.1.51 extracted from

Maisonneuve, S.; Bessoule, J.J.; Lessire, R.; Delseny, M.; Roscoe, T.J.
Mutagenesis of a plastidial lysophosphatidic acid acyltransferase (2000), Biochem. Soc. Trans., 28, 961-964.

Search for more literature for 2.3.1.51

Cloned(Commentary)

Cloned (Comment)	Organism
transformation of a modified plasmid into Escherichia coli JC201 enzyme-deficient strain and transformation of the plasmid DNA containing the cDNA BAT2 encoding the plastidial enzyme into the Escherichia coli XL-1 red mutator strain	Brassica napus

Protein Variants

Protein Variants	Comment	Organism
C247Y	loss of the ability of the plastidial enzyme to restore growth to JC201, suggests that C247 is essential for activity	Brassica napus
D199A	the mutant fails to grow beyond 0.04 D600 after transfer to the non-permissive temperature. D199 is essential for the ability of the plastidial enzyme to complement the defective acyltransferase activity of JC201	Brassica napus
E266A	the plastidial enzyme containing this mutation is unable to complement the temperature-sensitive phenotype of JC201, indicating an essential role for this acidic residue	Brassica napus
G140S	loss of the ability of the plastidial enzyme to restore growth to JC201, suggests that G140 is essential for activity	Brassica napus
H194A	the mutant fails to grow beyond 0.04 D600 after transfer to the non-permissive temperature. H194 is essential for the ability of the plastidial enzyme to complement the defective acyltransferase activity of JC201	Brassica napus
K84Q	greatly impaired growth rate, possibly indicating an importance of the N-terminus of the mature protein in determining enzyme activity	Brassica napus
L203P	loss of the ability of the plastidial enzyme to restore growth to JC201, suggests that L203 is essential for activity	Brassica napus
P187L	loss of the ability of the plastidial enzyme to restore growth to JC201, suggests that P187 is essential for activity	Brassica napus
R92G	greatly impaired growth rate, possibly indicating an importance of the N-terminus of the mature protein in determining enzyme activity	Brassica napus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
plastid	-	Brassica napus	9536	-

Organism

Organism	UniProt	Comment	Textmining
Brassica napus	Q9XFW4	-	-

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Release 2023.1 (January 2023)