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Literature summary for 2.3.1.51 extracted from

  • Kessels, J.M.M.; Ousen, H.; Van den Bosch, H.
    Facilitated utilization of endogenously synthesized lysophosphatidic acid by 1-acylglycerophosphate acyltransferase from Escherichia coli (1983), Biochim. Biophys. Acta, 753, 227-235.
    View publication on PubMed
Search for more literature for 2.3.1.51

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Escherichia coli 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate Escherichia coli involved in phospholipid biosynthesis CoA + 1,2-diacyl-sn-glycerol 3-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 K12, strains: LCD45 and CE1061
-

Purification (Commentary)

Purification (Comment) Organism
partial, separated from EC 2.3.1.15 using Triton X-100, the enzyme is not solubilized by Triton X-100 or cholate Escherichia coli

Renatured (Commentary)

Renatured (Comment) Organism
by mixing 1 vol. of the fraction containing the enzyme with 4 vol. of sonicated phosphatidylcholine/phosphatidylglycerol or total E. coli lipid vesicles Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0008
-
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate
-
 Escherichia coli CoA + 1,2-diacyl-sn-glycerol 3-phosphate i.e. phosphatidic acid ?
acyl-CoA + 1-acyl-sn-glycerol 3-phosphate involved in phospholipid biosynthesis Escherichia coli CoA + 1,2-diacyl-sn-glycerol 3-phosphate
-
 ?
acyl-CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
 Escherichia coli CoA + 1-palmitoyl-2-acyl-sn-glycerol 3-phosphate
-
 ?
acyl-CoA + linoleoyl-sn-glycerol 3-phosphate
-
 Escherichia coli CoA + ?
-
 ?
acyl-CoA + myristoyl-sn-glycerol 3-phosphate
-
 Escherichia coli CoA + ?
-
 ?
additional information lysophosphatidic acid, synthesized by EC 2.3.1.15, is utilized by the enzyme without prior mixing with the total membrane-associated pool of lysophosphatidic acid, and suggest a close proximity of the two enzymes in native E. coli membranes. This property of the acyltransferases is lost upon separation and reconstitution of enzyme activities Escherichia coli ?
-
 ?
oleoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
-
 Escherichia coli CoA + 1-acyl-2-oleoyl-sn-glycerol 3-phosphate
-
 ?
palmitoyl-CoA + 1-acyl-sn-glycerol 3-phosphate
-
 Escherichia coli CoA + 1-acyl-2-palmitoyl-sn-glycerol 3-phosphate
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
 assay at Escherichia coli