Information on EC 2.1.3.3 - ornithine carbamoyltransferase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea

EC NUMBER
COMMENTARY hide
2.1.3.3
-
RECOMMENDED NAME
GeneOntology No.
ornithine carbamoyltransferase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
carbamoyl phosphate + L-ornithine = phosphate + L-citrulline
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
carbamoyl group transfer
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
L-arginine biosynthesis I (via L-ornithine)
-
-
L-arginine biosynthesis II (acetyl cycle)
-
-
L-arginine biosynthesis IV (archaebacteria)
-
-
L-citrulline biosynthesis
-
-
L-citrulline degradation
-
-
L-proline biosynthesis II (from arginine)
-
-
urea cycle
Arginine biosynthesis
-
-
Metabolic pathways
-
-
Biosynthesis of secondary metabolites
-
-
Biosynthesis of antibiotics
-
-
SYSTEMATIC NAME
IUBMB Comments
carbamoyl-phosphate:L-ornithine carbamoyltransferase
The plant enzyme also catalyses the reactions of EC 2.1.3.6 putrescine carbamoyltransferase, EC 2.7.2.2 carbamate kinase and EC 3.5.3.12 agmatine deiminase, thus acting as putrescine synthase, converting agmatine [(4-aminobutyl)guanidine] and ornithine into putrescine and citrulline, respectively.
CAS REGISTRY NUMBER
COMMENTARY hide
9001-69-8
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
isoenzyme 1
-
-
Manually annotated by BRENDA team
3'-hydroxymethylcephem O-carbamoyltransferase
-
-
Manually annotated by BRENDA team
Arundinaria sp.
sugar cane, cytoplasmic and mitochondrial enzyme form
-
-
Manually annotated by BRENDA team
strain 168
-
-
Manually annotated by BRENDA team
strain 168
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
subsp. jejuni, gene argF
UniProt
Manually annotated by BRENDA team
subsp. jejuni, gene argF
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
loggerhead turtle
-
-
Manually annotated by BRENDA team
gene argF
-
-
Manually annotated by BRENDA team
carrot
-
-
Manually annotated by BRENDA team
blue-green alga
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
also known as Halobacterium salinarium, catabolic ornithine carbamoyltransferase
SwissProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain CETC4786T, ATCC 8290
-
-
Manually annotated by BRENDA team
strain Lp60, high assimilation of citrulline, low ornithine transcarbamylase activity
-
-
Manually annotated by BRENDA team
strain Lp60, high assimilation of citrulline, low ornithine transcarbamylase activity
-
-
Manually annotated by BRENDA team
putrescine synthase has inherent activities of agmatine iminohydrolase, putrescine carbamoyltransferase, ornithine carbamoyltransferase and carbamate kinase
-
-
Manually annotated by BRENDA team
gene lmo0036
-
-
Manually annotated by BRENDA team
gene lmo0036
-
-
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain PAO
-
-
Manually annotated by BRENDA team
strain PAO532
-
-
Manually annotated by BRENDA team
pv. phaseolicola
-
-
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
Rattus norvegicus Sprague-Dawley
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
shark
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
D10
-
-
Manually annotated by BRENDA team
D10
-
-
Manually annotated by BRENDA team
3'-hydroxymethylcephem O-carbamoyltransferase
-
-
Manually annotated by BRENDA team
house musk shrew
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
malfunction
metabolism
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3'-hydroxymethylcephem + carbamoyl phosphate
cephamycin C + phosphate
show the reaction diagram
arsenate + L-citrulline
carbamoylarsenate + L-ornithine
show the reaction diagram
-
pseudo-reverse reaction, arsenate is first coupled to citrulline, followed by elimination of carbamoylarsenate
-
r
carbamoyl phosphate + canaline
O-ureidohomoserine + phosphate
show the reaction diagram
carbamoyl phosphate + diaminobutane
phosphate + ?
show the reaction diagram
-
-
-
-
?
carbamoyl phosphate + L-lysine
phosphate + ?
show the reaction diagram
-
-
-
-
?
carbamoyl phosphate + L-Orn-Ala-hArg
phosphate + L-Cit-Ala-hArg
show the reaction diagram
carbamoyl phosphate + L-ornithine
L-citrulline + phosphate
show the reaction diagram
carbamoyl phosphate + L-ornithine
phosphate + citrulline
show the reaction diagram
carbamoyl phosphate + L-ornithine
phosphate + L-citrulline
show the reaction diagram
carbamoyl phosphate + lysine
phosphate + homocitrulline
show the reaction diagram
phosphate + L-citrulline
carbamoyl phosphate + L-ornithine
show the reaction diagram
-
-
-
-
r
phosphate + L-citrulline
carbamoylphosphate + L-ornithine
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
carbamoyl phosphate + canaline
O-ureidohomoserine + phosphate
show the reaction diagram
-
66fold higher activity with canaline than with ornithine, enzyme may play a role in canavanine synthesis
-
?
carbamoyl phosphate + L-Orn-Ala-hArg
phosphate + L-Cit-Ala-hArg
show the reaction diagram
carbamoyl phosphate + L-ornithine
L-citrulline + phosphate
show the reaction diagram
carbamoyl phosphate + L-ornithine
phosphate + citrulline
show the reaction diagram
P9WIT9
catalyzes the sixth step in arginine biosynthesis
-
-
r
carbamoyl phosphate + L-ornithine
phosphate + L-citrulline
show the reaction diagram
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,4-diaminobutane
2,4,6-Trinitrobenzenesulfonate
2,4-diaminobutyrate
-
-
2-mercaptoethanol
-
-
5,5'-dithiobis(2-nitrobenzoate)
5-hydroxy-2-aminovaleric acid
-
competitive vs. ornithine, uncompetitive vs. carbamoylphosphate
Acetylimidazole
-
50 mM, 95% inhibition at 25C in 100 mM imidazole-HCl, pH 7.5
agmatine
-
13 mM, 50% inhibition
arginase
-
500 units, 14% remaining activity
-
arginine
arsenate
-
competitive vs. carbamoylphosphate, noncompetitive vs. ornithine
Carbamoyl phosphate
Cd2+
-
1 mM, 99% inhibition
Chlorosis-inducing toxin of Pseudomonas phaseolicola
-
no more than 85% inhibition
-
citrulline
-
competitive to carbamoyl phosphate
Cu2+
-
1 mM, 75% inhibition
cystamine
delta-N-(phosphonacetyl)-L-ornithine
delta-N-(phosphonoacetyl)-L-ornithine
bisubstrate analogue inhibitor
Delta-N-Phosphonoacetyl-L-ornithine
diaminobutyric acid
-
-
dithiodiglycol
-
100 mM, inactivation at pH 9,0
dithiothreitol
-
-
DL-2-Amino-5-hydroxypentanoic acid
glutamine
-
-
glycine
-
-
histidine
-
-
iodoacetate
L-2,4-diaminobutyric acid
L-2-Amino-4-pentenoic acid
-
-
L-2-aminobutyrate
L-alanine
L-asparagine
-
weak
L-cysteine
-
10 mM, 90% inhibition, noncompetitive vs. ornithine
L-isoleucine
L-leucine
L-lysine
L-norleucine
L-norvaline
L-ornithine
L-serine
-
weak
L-valine
methionine
MgCl2
-
10 mM, 14% inhibition
N-delta-(phosphosulfamyl)ornithylalanylhomoarginine
NaCl
-
50 mM, 25% inhibition
ornithine
ornithine-delta-P(O)(NH2)-NH-SO3H
-
inhibition of isoform 1 only
-
p-chloromercuribenzoate
-
-
p-hydroxymercuribenzoate
Phenylglyoxal
-
complete inactivation after 1 h incubation
phosphate
S-carbamoyl cysteine
-
10 mM, 90% inhibition
spermidine
Tris
-
50 mM, more than 80% inhibition
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ADP
-
10 mM, 350% arsenolytic cleavage activity
arginine
50 mM, 2fold activatin
arsenate
-
potent activator in the concentration range of 0-10 mM
CTP
-
10 mM, 340% arsenolytic cleavage activity
phosphate
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0008 - 1.24
Carbamoyl phosphate
0.033 - 0.25
Carbamoylphosphate
43.5
Diaminobutane
-
pH 8.5, 37C
10.4
L-citrulline
-
pH 8.0, 37C
25
L-lysine
-
pH 8.5, 37C
0.001 - 350
L-ornithine
0.8 - 1.85
ornithine
2.3
phosphate
-
pH 8.0, 37C
additional information
additional information
-
enzyme folding/unfolding kinetics and thermodynamics, overview
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
82
Carbamoyl phosphate
Giardia intestinalis
-
pH 8.0, 37C
0.025
Diaminobutane
Pseudomonas savastanoi
-
pH 8.5, 37C
8.1 - 90
L-citrulline
0.0036
L-lysine
Pseudomonas savastanoi
-
pH 8.5, 37C
49 - 750
L-ornithine
18 - 4300
ornithine
7.9
phosphate
Giardia intestinalis
-
pH 8.0, 37C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
650000
Carbamoyl phosphate
Giardia intestinalis
-
pH 8.0, 37C
440
780
L-citrulline
Giardia intestinalis
-
pH 8.0, 37C
938
880000
L-ornithine
Giardia intestinalis
-
pH 8.0, 37C
192
3400
phosphate
Giardia intestinalis
-
pH 8.0, 37C
16
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
7
2,4-diaminobutyrate
-
-
8.2
arginine
-
determined in the presence of 0.5 mM ornithine and 0.5 mM carbamoylphosphate
0.012 - 0.151
Carbamoyl phosphate
0.00001 - 0.000025
delta-N-(phosphonacetyl)-L-ornithine
0.27
delta-N-(phosphonoacetyl)-L-ornithine
-
at 30C and pH 7.4, competitive vs. carbamoyl phosphate, noncompetitive vs. ornithine
0.0000047 - 0.0115
Delta-N-Phosphonoacetyl-L-ornithine
1.1
diaminobutyric acid
-
pH 8.5, 37C
15
glycine
-
-
13
L-alanine
-
-
0.58
L-alpha-aminobutyrate
-
-
110
L-asparagine
-
-
40
L-histidin
-
-
10.5 - 14
L-isoleucine
1.2 - 5.5
L-leucine
40
L-methionine
-
-
0.071 - 1.7
L-norvaline
1.9
L-ornithine
-
pH 8.5, 37C
63.5
L-serine
-
-
12.5 - 13.5
L-valine
0.0002 - 0.0009
N-delta-(phosphosulfamyl)ornithylalanylhomoarginine
0.06 - 3.6
ornithine
0.25 - 8
phosphate
0.01
phosphonacetyl-L-ornithine
-
between pH 7.5-9.0
-
additional information
additional information
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00058
-
crude cell extract
0.0657
-
purified enzyme
0.0928
-
isoenzyme 2 with ornithine
0.255
-
pH 8.0, 55C, activity in cell culture in minimal growth medium with 20 mM glucose and 5 mM NH4+
0.43
-
pH 8.0, 55C, activity in cell culture in growth medium with 0.5% peptone and 0.1% yeast extract
0.47
-
multifunctional enzyme
0.574
-
isoenzyme 1 with canaline
1.24
-
isoenzyme 2 with canaline
1.28
-
mutant T68G, pH 8.0, 30C
2.33
-
extracts prepared from Escherichia coli CM236 carrying pME178
2.83
-
mutant K289S, pH 8.0, 30C
3.33
-
recombinant enzyme
3.59
-
anabolic reaction, pH 10.0, 37C
4.16
-
mutant C191M/C194N/F197V, pH 8.0, 30C
4.66
-
mutant L290S, pH 8.0, 30C
5.56
-
isoenzyme 1
7.5
-
mutant K263A, pH 8.0, 30C
8.31
-
isoenzyme 1 with ornithine
12.33
-
mutant N184Q, pH 8.0, 30C
12.66
-
mutant E256Q, pH 8.0, 30C
12.67
-
pH 7.5, crude cell exstract
13.3
-
mutant L290Q, pH 8.0, 30C
14.83
-
mutant K263R, pH 8.0, 30C
15
-
mutant E256A, pH 8.0, 30C
16.16
-
mutant D182N, pH 8.0, 30C
16.83
-
mutant K265A, pH 8.0, 30C
23.38
-
mutant E123S, pH 8.0, 30C
23.63
-
mutant K265R, pH 8.0, 30C
24
recombinant wild-type enzyme, substrate L-ornithine, pH 8.5, 37C
25
-
mutant K268A, pH 8.0, 30C; mutant N185Q, pH 8.0, 30C
25.16
-
mutant K260R, pH 8.0, 30C
25.3
-
mutant E123A, pH 8.0, 30C
25.73
-
mutant K268R, pH 8.0, 30C
25.83
-
mutant K260A, pH 8.0, 30C
26.12
-
wild-type, pH 8.0, 30C
26.4
-
-
40.2
-
recombinant enzyme
48.66
-
mutant Q294P, pH 8.0, 30C
82
recombinant enzyme mutant Y230V/G231S/L232M/Y233G, substrate L-ornithine, pH 8.5, 37C
83.3
-
enzyme from a liver of a Reye's syndrome patient
86.5
-
enzyme from normal liver
157.8
-
-
250 - 260
-
-
450
-
pH 7.5, purified enzyme
780
-
-
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 6
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catabolic reaction
6.7
-
in potassium phosphate buffer 5 mM ornithine, pH optimum shifts to more alkaline values with decreasing ornithine concentrations
7.1
-
ornithine synthesis
7.2
-
at 10 mM carbamoyl phosphate, sharp decrease of activity above
7.25
-
wild type
7.3
-
catabolic ornithine carbamoyltransferase
7.4
-
assay at
7.6
-
triethanolamine/acetate buffer
7.7
-
triethanolamine buffer, 85% of maximal activity at pH 8.5, 25% of maximal activity at pH 6.8
7.9
-
HEPES/NaOH buffer
8.4 - 8.8
-
-
8.5 - 9.5
-
phaseolotoxin sensitive isoform 1
8.6
-
at 0.025 mM ornithine
8.8
30% of maximal activity at pH 7.0
10
-
anabolic reaction
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 11
-
activity range, anabolic and catabolic reactions
6 - 8.3
-
approx. 50% of maximal activity at pH 6.0
6 - 7.2
-
at 10 mM carbamoyl phosphate, sharp decrease in activity above
6.7 - 8.5
-
approx. 50% of maximal activity at pH 6.7 and 8.5, triethanolamine/acetate buffer
6.8 - 8.5
-
approx. 85% of maximal activity at pH 6.8, approx. 25% of maximal activity at pH 8.5
7 - 9
-
50% of maximal activity at pH 9.0, 70% at pH 7.0
7 - 10
-
sharp decrease in activity below pH 9.0
7.2 - 8.3
-
71% of maximal activity at pH 7.2, 94% of maximal activity at pH 8.3, HEPES/NaOH buffer
7.5 - 10
-
nearly constant activity in this range, half-maximal activity at pH 7.0, negliable activity at pH 6.0
7.5 - 9
-
approx. 50% of maximal activity at pH 9.0
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
23 - 25
-
-
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
i.e. a larval salmonid that has hatched but has not fully absorbed its yolk sac
Manually annotated by BRENDA team
-
germinating
Manually annotated by BRENDA team
-
serum level of the enzyme, leakage and clearance, is influenced by the activation state of Kupffer cells, overview
Manually annotated by BRENDA team
-
of conidia
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
serum, from leakage of liver
-
Manually annotated by BRENDA team
PDB
SCOP
CATH
ORGANISM
UNIPROT
Brucella melitensis biotype 2 (strain ATCC 23457)
Burkholderia thailandensis (strain ATCC 700388 / DSM 13276 / CIP 106301 / E264)
Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)
Coccidioides immitis (strain RS)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Gloeobacter violaceus (strain PCC 7421)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycoplasma penetrans (strain HF-2)
Mycoplasma penetrans (strain HF-2)
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Vibrio vulnificus (strain CMCP6)
Vibrio vulnificus (strain CMCP6)
Vibrio vulnificus (strain CMCP6)
Vibrio vulnificus (strain CMCP6)
Vibrio vulnificus (strain CMCP6)
Vibrio vulnificus (strain CMCP6)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
38000
-
-
56000
-
multifunctional enzyme with agmatine iminohydrolase, putrescine carbamoyltransferase, ornithine carbamoyltransferase and carbamate kinase activity, gel filtration
77600
-
gel filtration
79000
Arundinaria sp.
-
cytoplasmic form
87000
-
gel filtration
95000
-
gel filtration
98000
-
sedimentation equilibrium
102000
-
gel filtration