Literature summary for 2.1.3.3 extracted from

Bellocco, E.; Di Salvo, C.; Lagana, G.; Galtieri, A.; Ficarra, S.; Kotyk, A.; Leuzzi, U.
Ornithine carbamoyltransferase from Spinacia oleracea: purification and characterization (2002), Biol. Plant., 45, 533-538.

No PubMed abstract available

Search for more literature for 2.1.3.3

Inhibitors

Inhibitors	Comment	Organism	Structure
ornithine	competitive vs. carbamoylphosphate in the presence of phosphate	Spinacia oleracea

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
40000	-	3 * 40000, SDS-PAGE	Spinacia oleracea
118000	-	gel filtration	Spinacia oleracea

Organism

Organism	UniProt	Comment	Textmining
Spinacia oleracea	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
ammonium sulfate, heat, activated charcoal, delta-N-(phosphonacetyl)-L-ornithine affinity chromatography	Spinacia oleracea

Reaction

Reaction	Comment	Organism	Reaction ID
carbamoyl phosphate + L-ornithine = phosphate + L-citrulline	ordered bi-bi-sequential reaction mechanism, carbamoylphosphate binds first followed by L-ornithine, L-citrulline leaves first followed by phosphate	Spinacia oleracea	a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
436	-	-	Spinacia oleracea

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
carbamoyl phosphate + L-ornithine	-	Spinacia oleracea	phosphate + L-citrulline	-	?

Subunits

Subunits	Comment	Organism
trimer	3 * 40000, SDS-PAGE	Spinacia oleracea

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
56	-	half-live: 99 min	Spinacia oleracea
58	-	half-life: 60 min	Spinacia oleracea
64	-	half-life: 11 min	Spinacia oleracea
68	-	half-life: 10.5 min, half-life increases to 45 min in the presence of 50 mM ornithine	Spinacia oleracea

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Release 2023.1 (January 2023)