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Literature summary for 2.1.3.3 extracted from

  • Lee, Y.; Jun, B.O.; Kim, S.G.; Kwon, Y.M.
    Purification of ornithine carbamyltransferase from kidney bean (Phaseolus vulgaris) leaves and comparison of the properties of the enzyme from canavanine-containing and -deficient plants (1998), Planta, 205, 375-379.
    View publication on PubMed
Search for more literature for 2.1.3.3

Inhibitors

Inhibitors Comment Organism Structure
L-cysteine 10 mM, 90% inhibition, noncompetitive vs. ornithine Phaseolus vulgaris
S-carbamoyl cysteine 10 mM, 90% inhibition Phaseolus vulgaris
Tris 50 mM, more than 80% inhibition Pisum sativum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
 3 * 36000, SDs-PAGE Phaseolus vulgaris
109000
-
-
 Canavalia lineata
109000
-
 gel filtration Phaseolus vulgaris

Organism

Organism UniProt Comment Textmining
Brassica juncea
-
-
-
Canavalia brasiliensis
-
-
-
Canavalia gladiata
-
-
-
Canavalia lineata
-
-
-
Canavalia rosea
-
-
-
Glycine max
-
-
-
Lablab purpureus
-
-
-
Macropsychanthus megacarpus
-
-
-
Mus musculus
-
-
-
Oryctolagus cuniculus
-
-
-
Perilla frutescens
-
-
-
Phaseolus vulgaris
-
-
-
Pisum sativum
-
-
-
Solanum lycopersicum
-
-
-
Solanum melongena
-
-
-
Wisteria floribunda
-
-
-

Purification (Commentary)

Purification (Comment) Organism
delta-N-(phosphonoacetyl)-L-ornithine-Sepharose 6B affinity chromatography Phaseolus vulgaris

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Phaseolus vulgaris
-
leaf
-
 Pisum sativum
-
leaf
-
 Glycine max
-
leaf
-
 Solanum lycopersicum
-
leaf
-
 Solanum melongena
-
leaf
-
 Brassica juncea
-
leaf
-
 Lablab purpureus
-
leaf
-
 Perilla frutescens
-
leaf
-
 Canavalia lineata
-
leaf
-
 Canavalia rosea
-
leaf
-
 Canavalia gladiata
-
leaf
-
 Canavalia brasiliensis
-
leaf
-
 Macropsychanthus megacarpus
-
leaf
-
 Wisteria floribunda
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
526
-
-
 Phaseolus vulgaris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carbamoyl phosphate + L-ornithine
-
 Mus musculus phosphate + L-citrulline
-
 ?
carbamoyl phosphate + L-ornithine
-
 Oryctolagus cuniculus phosphate + L-citrulline
-
 ?
carbamoyl phosphate + L-ornithine
-
 Pisum sativum phosphate + L-citrulline
-
 ?
carbamoyl phosphate + L-ornithine
-
 Glycine max phosphate + L-citrulline
-
 ?
carbamoyl phosphate + L-ornithine
-
 Solanum lycopersicum phosphate + L-citrulline
-
 ?
carbamoyl phosphate + L-ornithine
-
 Solanum melongena phosphate + L-citrulline
-
 ?
carbamoyl phosphate + L-ornithine
-
 Brassica juncea phosphate + L-citrulline
-
 ?
carbamoyl phosphate + L-ornithine
-
 Lablab purpureus phosphate + L-citrulline
-
 ?
carbamoyl phosphate + L-ornithine
-
 Perilla frutescens phosphate + L-citrulline
-
 ?
carbamoyl phosphate + L-ornithine
-
 Canavalia lineata phosphate + L-citrulline
-
 ?
carbamoyl phosphate + L-ornithine
-
 Canavalia rosea phosphate + L-citrulline
-
 ?
carbamoyl phosphate + L-ornithine
-
 Canavalia gladiata phosphate + L-citrulline
-
 ?
carbamoyl phosphate + L-ornithine
-
 Canavalia brasiliensis phosphate + L-citrulline
-
 ?
carbamoyl phosphate + L-ornithine
-
 Macropsychanthus megacarpus phosphate + L-citrulline
-
 ?
carbamoyl phosphate + L-ornithine
-
 Wisteria floribunda phosphate + L-citrulline
-
 ?
carbamoyl phosphate + L-ornithine very low activity with canaline Phaseolus vulgaris phosphate + L-citrulline
-
 ?

Subunits

Subunits Comment Organism
trimer
-
 Pisum sativum
trimer
-
 Canavalia lineata
trimer 3 * 36000, SDs-PAGE Phaseolus vulgaris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Pisum sativum
8
-
-
 Canavalia lineata