Cloned (Comment) | Organism |
---|---|
gene MB1684, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS | Mycobacterium tuberculosis variant bovis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39000 | - |
x * 39000, untagged enzyme, SDS-PAGE, x * 41000, recombinant His-tagged enzyme, SDS-PAGE | Mycobacterium tuberculosis variant bovis |
41000 | - |
x * 39000, untagged enzyme, SDS-PAGE, x * 41000, recombinant His-tagged enzyme, SDS-PAGE | Mycobacterium tuberculosis variant bovis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
carbamoyl phosphate + L-ornithine | Mycobacterium tuberculosis variant bovis | - |
phosphate + L-citrulline | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis variant bovis | P0A5M9 | gene MB1684 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography | Mycobacterium tuberculosis variant bovis |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
carbamoyl phosphate + L-ornithine | - |
Mycobacterium tuberculosis variant bovis | phosphate + L-citrulline | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 39000, untagged enzyme, SDS-PAGE, x * 41000, recombinant His-tagged enzyme, SDS-PAGE | Mycobacterium tuberculosis variant bovis |
Synonyms | Comment | Organism |
---|---|---|
carbamoyltransferase | - |
Mycobacterium tuberculosis variant bovis |
MB1684 | - |
Mycobacterium tuberculosis variant bovis |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme induces proinflammatory cytokine gene expression by activating NF-kappaB in macrophages of the bovine host. The enzyme plays a role in induction of upregulation of interferon-gamma and proinflammatory cytokines (interleukin-1beta, IL-6, and tumor necrosis factor-alpha) in Bos taurus Ana-1 macrophages | Mycobacterium tuberculosis variant bovis |