6.3.4.19: tRNAIle-lysidine synthase

This is an abbreviated version!
For detailed information about tRNAIle-lysidine synthase, go to the full flat file.

Reaction

Synonyms

lysidine synthetase, mesJ, MesJ protein, TilS, TilS protein, tRNAIle lysidine synthetase, tRNAIle-lysidine synthetase, yacA

ECTree

     6 Ligases

         6.3 Forming carbon-nitrogen bonds

             6.3.4 Other carbon-nitrogen ligases

                6.3.4.19 tRNAIle-lysidine synthase

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Results	in table
43164	AA Sequence
3	Application
1	CAS Registry Number
7	Cloned(Commentary)
4	Crystallization (Commentary)
10	General Information
23	IC50 Value
23	Inhibitors
2	kcat/KM [mM/s]
33	KM Value [mM]
11	Natural Substrates/ Products (Substrates)
116	Organism
1	pH Optimum
14	Protein Variants
5	Purification (Commentary)
1	Reaction
16	Reference
27	Substrates and Products (Substrate)
2	Subunits
25	Synonyms
1	Systematic Name
7	Turnover Number [1/s]

Natural Substrates Products

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Natural Substrates Products on EC 6.3.4.19 - tRNAIle-lysidine synthase

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NATURAL SUBSTRATE

NATURAL PRODUCT

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

REVERSIBILITY
r=reversible
ir=irreversible
?=not specified

[tRNAIle2]-cytidine34 + L-lysine + ATP

[tRNAIle2]-2-L-lysylcytidine34 + AMP + diphosphate

8 entries

[tRNAIle2]-cytidine34 + L-lysine + ATP

[tRNAIle2]-lysidine34 + AMP + diphosphate + H2O

2 entries

[tRNAIle2]-cytidine34 + L-lysine + ATP

[tRNAIle2]-2-L-lysylcytidine34 + AMP + diphosphate

Aquifex aeolicus

O67728

-

706481

-

-

?

[tRNAIle2]-cytidine34 + L-lysine + ATP

[tRNAIle2]-2-L-lysylcytidine34 + AMP + diphosphate

Bacillus subtilis

P37563

the enzyme generates lysidine at the wobble position of tRNAIle. The enzyme is essential for the decoding of AUA codons in the cell

705717

-

-

?

[tRNAIle2]-cytidine34 + L-lysine + ATP

[tRNAIle2]-2-L-lysylcytidine34 + AMP + diphosphate

Escherichia coli

-

-

705718, 706481

-

-

?

[tRNAIle2]-cytidine34 + L-lysine + ATP

[tRNAIle2]-2-L-lysylcytidine34 + AMP + diphosphate

Escherichia coli

P52097

-

703735

-

-

?

[tRNAIle2]-cytidine34 + L-lysine + ATP

[tRNAIle2]-2-L-lysylcytidine34 + AMP + diphosphate

Escherichia coli

P52097

bacteria decode the isoleucine codon AUA using a tRNA species that is posttranscriptionally modified at the wobble position of the anticodon with a lysine-containing cytidine derivative called lysidine. The lysidine modification of tRNAIle2 is an essential identity determinant for proper aminoacylation by isoleucyl tRNA synthetase and codon recognition on the ribosome. The ATP- and lysine-dependent formation of lysidine is catalyzed by tRNAIle-lysidine synthetase

704642

-

-

?

[tRNAIle2]-cytidine34 + L-lysine + ATP

[tRNAIle2]-2-L-lysylcytidine34 + AMP + diphosphate

Escherichia coli

-

enzyme catalyzes the lysylation of C34 (wobble position) in the precursor tRNAIle(CAU), thereby leading to the formation of tRNAIle(lysidineAU). The formation of lysidine by this essential enzyme allows recognition of tRNAIle(lysidineCAU) by Ile-tRNA synthetase and switches the base pairing of the tRNA from AUG (Met) to AUA (Ile)

706867

-

-

?

[tRNAIle2]-cytidine34 + L-lysine + ATP

[tRNAIle2]-2-L-lysylcytidine34 + AMP + diphosphate

Escherichia coli

-

the enzyme generates lysidine at the wobble position of tRNAIle. The enzyme is essential for the decoding of AUA codons in the cell

705717

-

-

?

[tRNAIle2]-cytidine34 + L-lysine + ATP

[tRNAIle2]-2-L-lysylcytidine34 + AMP + diphosphate

Geobacillus kaustophilus

Q5L3T3

-

705888

-

-

?

[tRNAIle2]-cytidine34 + L-lysine + ATP

[tRNAIle2]-lysidine34 + AMP + diphosphate + H2O

Bacillus subtilis

-

-

728408

-

-

?

[tRNAIle2]-cytidine34 + L-lysine + ATP

[tRNAIle2]-lysidine34 + AMP + diphosphate + H2O

Bacillus subtilis 168

-

-

728408

-

-

?