Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

6.3.4.19: tRNAIle-lysidine synthase

This is an abbreviated version!
For detailed information about tRNAIle-lysidine synthase, go to the full flat file.

Word Map on EC 6.3.4.19

Reaction

[tRNAIle2]-cytidine34
+
L-lysine
 +
ATP
=
[tRNAIle2]-lysidine34
 +
AMP
 +
diphosphate
 +
H2O

Synonyms

lysidine synthetase, mesJ, MesJ protein, TilS, TilS protein, tRNAIle lysidine synthetase, tRNAIle-lysidine synthetase, yacA

ECTree

     6 Ligases
         6.3 Forming carbon-nitrogen bonds
             6.3.4 Other carbon-nitrogen ligases
                6.3.4.19 tRNAIle-lysidine synthase

Engineering

Engineering on EC 6.3.4.19 - tRNAIle-lysidine synthase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D137A
no activity detectable
D191A
no activity detectable
D36A
no activity detectable
E140A
Km-value for L-lysine is 2.2fold higher than wild-type value, Km-value for ATP is about 7fold higher than wild-type value, Km-value for [tRNAIle2]-cytidine34 is 4.7fold higher than wild-type value
H133A
Km-value for L-lysine is 1.6 fold higher than wild-type value, Km-value for ATP is 2.5fold lower than wild-type value, Km-value for [tRNAIle2]-cytidine34 is similar to wild-type value
N194A
no activity detectable
R113A
no activity detectable
R174A
Km-value for L-lysine is 1.8fold higher than wild-type value, Km-value for ATP is 2.1fold higher than wild-type value, Km-value for [tRNAIle2]-cytidine34 is 6fold higher than wild-type value
R201A
no activity detectable
R205A
Km-value for L-lysine is 1.6fold higher than wild-type value, Km-value for ATP is 14.8fold higher than wild-type value, Km-value for [tRNAIle2]-cytidine34 is 3.6fold higher than wild-type value
S37A
Km-value for ATP is 13fold higher than wild-type value
W188A
Km-value for ATP is 5fold higher than wild-type value
Y114A
Km-value for L-lysine is about 5fold higher than wild-type value, Km-value for ATP is about 8fold higher than wild-type value, Km-value for [tRNAIle2]-cytidine34 is 2.2fold higher than wild-type value
additional information
-
a mutation study reveals that Escherichia coli TilS discriminates tRNAIle from the structurally similar tRNAMet having the same anticodon loop by recognizing the anticodon loop, the anticodon stem, and the acceptor stem