Application | Comment | Organism |
---|---|---|
medicine | lysidine/TilS is a eubacterial-specific system of AUA decoding. Therefore, TilS is an ideal target for a broad-spectrum antibacterial agent | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
[tRNAIle2]-cytidine34 + L-lysine + ATP | Escherichia coli | - |
[tRNAIle2]-2-L-lysylcytidine34 + AMP + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P52097 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
[tRNAIle2]-cytidine34 + L-lysine + ATP | - |
Escherichia coli | [tRNAIle2]-2-L-lysylcytidine34 + AMP + diphosphate | - |
? | |
[tRNAIle2]-cytidine34 + L-lysine + ATP | TilS specifically interacts with the isolated Escherichia coli tRNAIle2. Molecular mechanism of lysidine formation consists of two consecutive reactions involving the adenylated tRNA intermediate. TilS activates the C-2 position of C34 by forming an adenylate intermediate. Second, nucleophilic attack of the C-2 position of the intermediate by the epsilon-amino group of lysine completes the reaction | Escherichia coli | [tRNAIle2]-2-L-lysylcytidine34 + AMP + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TilS | - |
Escherichia coli |
tRNAIle lysidine synthetase | - |
Escherichia coli |
General Information | Comment | Organism |
---|---|---|
physiological function | the single 2-lysylcytidine modification converts the codon-specificity from AUG to AUA, and the amino acid specificity from Met to Ile | Escherichia coli |