6.3.2.30: cyanophycin synthase (L-arginine-adding)
This is an abbreviated version!
For detailed information about cyanophycin synthase (L-arginine-adding), go to the full flat file.
Word Map on EC 6.3.2.30
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6.3.2.30
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synechocystis
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cyanobacteria
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anabaena
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cyanophycinase
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synthesis
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eutropha
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elongatus
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thermosynechococcus
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multi-l-arginyl-poly-l-aspartic
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heterocysts
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nitrogen-rich
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desulfitobacterium
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non-cyanobacterial
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hafniense
- 6.3.2.30
- synechocystis
- cyanobacteria
- anabaena
- cyanophycinase
- synthesis
- eutropha
- elongatus
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thermosynechococcus
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multi-l-arginyl-poly-l-aspartic
- heterocysts
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nitrogen-rich
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desulfitobacterium
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non-cyanobacterial
- hafniense
Reaction
Synonyms
Ava_0335, CGP synthetase, CphA, CphA1, CphA2, CphA49, CphANE1, cyanophycin synthetase, More, Tlr2170 protein
ECTree
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Substrates Products
Substrates Products on EC 6.3.2.30 - cyanophycin synthase (L-arginine-adding)
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REACTION DIAGRAM
ATP + [L-Asp(4-L-Arg)]n + alpha-L-Asp-L-Arg
ADP + phosphate + [L-Asp(4-L-Arg)]n+1
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ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg
[L-Asp(4-L-Arg)]n+1 + ADP + phosphate
L-Lys cannot replace L-Arg in reaction with CphANE1 or CphANE1del96
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ADP + phosphate + [L-Asp(4-L-Arg)]n+1
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ATP + [L-Asp(4-L-Arg)]n + beta-L-Asp-L-Arg
ADP + phosphate + [L-Asp(4-L-Arg)]n+1
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?
ADP + phosphate + [L-Asp(4-L-Arg)]n+1
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ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg
ADP + phosphate + [L-Asp(4-L-Arg)]n+1
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?
ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg
ADP + phosphate + [L-Asp(4-L-Arg)]n+1
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ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg
ADP + phosphate + [L-Asp(4-L-Arg)]n+1
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ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg
ADP + phosphate + [L-Asp(4-L-Arg)]n+1
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study of the effect of different light and nutrition conditions on cyanophycin granule formation
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ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg
ADP + phosphate + [L-Asp(4-L-Arg)]n+1
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ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg
ADP + phosphate + [L-Asp(4-L-Arg)]n+1
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ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg
ADP + phosphate + [L-Asp(4-L-Arg)]n+1
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role of the C-terminal region of CphANE1, Glu856 is critical for CphANE1 catalytic activity, overview
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ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg
ADP + phosphate + [L-Asp(4-L-Arg)]n+1
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ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg
ADP + phosphate + [L-Asp(4-L-Arg)]n+1
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role of the C-terminal region of CphANE1, Glu856 is critical for CphANE1 catalytic activity, overview
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ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg
ADP + phosphate + [L-Asp(4-L-Arg)]n+1
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study of expression profiles of the genes cphA1 and cphA2 and their dependence on the type of nitrogen supply in the medium
[L-Asp(4-L-Arg)]n-Asp is the substrate for the second reaction catalysed by cyanophycin synthase
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ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg
ADP + phosphate + [L-Asp(4-L-Arg)]n+1
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ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg
ADP + phosphate + [L-Asp(4-L-Arg)]n+1
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ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg
ADP + phosphate + [L-Asp(4-L-Arg)]n+1
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ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg
ADP + phosphate + [L-Asp(4-L-Arg)]n+1
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a small amount of cyanophycin is required as a primer
no formation of AMP, [L-Asp(3-L-Arg)]n-Asp is the substrate for the second reaction catalysed by cyanophycin synthase
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ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg
ADP + phosphate + [L-Asp(4-L-Arg)]n+1
normal assay conditions
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ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg
ADP + phosphate + [L-Asp(4-L-Arg)]n+1
Thermosynechococcus vestitus
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ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg
ADP + phosphate + [L-Asp(4-L-Arg)]n+1
Thermosynechococcus vestitus
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primer and product analysis using recombinantly expressed Tlr2170 protein, overview
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ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg
ADP + phosphate + [L-Asp(4-L-Arg)]n+1
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a small amount of cyanophycin is required as a primer
no formation of AMP, [L-Asp(4-L-Arg)]n-Asp is the substrate for the second reaction catalysed by cyanophycin synthase
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?
ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg
ADP + phosphate + [L-Asp(4-L-Arg)]n+1
a small amount of cyanophycin is required as a primer
no formation of AMP, [L-Asp(4-L-Arg)]n-Asp is the substrate for the second reaction catalysed by cyanophycin synthase, EC 6.3.2.30
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ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg
ADP + phosphate + [L-Asp(4-L-Arg)]n+1
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a short peptide is required as a primer, the primer peptide is elongated at its C-terminus
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ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg
ADP + phosphate + [L-Asp(4-L-Arg)]n+1
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poly-L-aspartic acid + ADP + phosphate
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L-aspartic acid + ATP
poly-L-aspartic acid + ADP + phosphate
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without L-aspartic acid 9.2% activity compared to the reaction mixture containing both substrates, no activity using L-glutamic acid instead of L-aspartic acid, no activity without addition of small amounts of cyanophycin as a primer for synthesis
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additional information
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cyanophycin accumulation is studied, comparison of nitrogen-fixing and non-nitrogen-fixing cyanobacteria
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additional information
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the enzyme catalyzes the two-step reaction performing reactions of EC 6.3.2.30 and EC 6.3.2.29
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additional information
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the enzyme catalyzes the two-step reaction performing reactions of EC 6.3.2.30 and EC 6.3.2.29
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additional information
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negligible activities when L-aspartic acid is omitted
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additional information
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no activity without L-aspartic acid, L-glutamic acid cannot substitute for L-aspartic acid
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additional information
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Thermosynechococcus vestitus
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CphA requires L-Asp, L-Arg, ATP, Mg2+, and low-molecular mass cyanophycin as a primer for cyanophycin synthesis and catalyzes the elongation of a low-molecular mass cyanophycin, overview
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additional information
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Thermosynechococcus vestitus
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the enzyme catalyzes the two-step reaction performing reactions of EC 6.3.2.30 and EC 6.3.2.29. Recombinant Tlr2170 protein catalyzes in vitro cyanophycin synthesis in the absence of a primer. The Tlr2170 protein shows strict substrate specificity toward L-Asp and L-Arg
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additional information
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no product is formed if the peptide primer is blocked at the C-terminus or if L-arginine is solely supplied as substrate
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additional information
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enzyme is specific for the dipeptide beta-aspartyl-arginine and ATP as substrates. To incorporate 1 mol beta-aspartyl-arginine into cyanophycin, the hydrolysis of about 1 mol ATP is required. No substrates: beta-aspartyl-lysine, alpha-aspartyl-glycine, beta-aspartyl-glycine, alpha-aspartyl-leucine, beta-aspartyl-leucine, beta-aspartyl-alanine, beta-aspartyl-phenylalanine, alpha-glutamyl-leucine, alpha-alanyl-glycine, L-aspartate, L-arginine
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additional information
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cyanophycin synthetase catalyzes the synthesis of cyanophycin by ATP-dependent polymerization of L-Asp and L-Arg. In vitro, the activity of CphA generally depends on the presence of L-Asp, L-Arg, ATP, Mg2+, K+, sulfhydryl compound, and cyanophycin as primers
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additional information
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the recombinant CphA49 exhibits strict primer dependency and broad substrate specificities. L-Lys and L-Glu can substitute for L-Arg, but with very low catalytic activity. No activity with L-Arg and L-citrulline, L-Arg and L-ornithine, L-Asp and L-citrulline, and L-Asp and L-ornithine
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