Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21(DE3) | Trichormus variabilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg | Trichormus variabilis | a small amount of cyanophycin is required as a primer | ADP + phosphate + [L-Asp(4-L-Arg)]n+1 | no formation of AMP, [L-Asp(4-L-Arg)]n-Asp is the substrate for the second reaction catalysed by cyanophycin synthase | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trichormus variabilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Trichormus variabilis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg = ADP + phosphate + [L-Asp(4-L-Arg)]n+1 | i.e. the first reaction of cyanophycin synthesis, the second reaction is catalysed by activity EC 6.3.2.29, sequence analysis suggests that cyanophycin synthase has two ATP binding sites corresponding to two active sites | Trichormus variabilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg | a small amount of cyanophycin is required as a primer | Trichormus variabilis | ADP + phosphate + [L-Asp(4-L-Arg)]n+1 | no formation of AMP, [L-Asp(4-L-Arg)]n-Asp is the substrate for the second reaction catalysed by cyanophycin synthase | ? | |
ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg | a short peptide is required as a primer, the primer peptide is elongated at its C-terminus | Trichormus variabilis | ADP + phosphate + [L-Asp(4-L-Arg)]n+1 | - |
? | |
L-aspartic acid + ATP | - |
Trichormus variabilis | poly-L-aspartic acid + ADP + phosphate | - |
? | |
additional information | no product is formed if the peptide primer is blocked at the C-terminus or if L-arginine is solely supplied as substrate | Trichormus variabilis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CphA | - |
Trichormus variabilis |
cyanophycin synthetase | - |
Trichormus variabilis |