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Literature summary for 6.3.2.30 extracted from

  • Berg, H.; Ziegler, K.; Piotukh, K.; Baier, K.; Lockau, W.; Volkmer-Engert, R.
    Biosynthesis of the cyanobacterial reserve polymer multi-L-arginyl-poly-L-aspartic acid (cyanophycin) (2000), Eur. J. Biochem., 267, 5561-5570.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21(DE3) Trichormus variabilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg Trichormus variabilis a small amount of cyanophycin is required as a primer ADP + phosphate + [L-Asp(4-L-Arg)]n+1 no formation of AMP, [L-Asp(4-L-Arg)]n-Asp is the substrate for the second reaction catalysed by cyanophycin synthase ?

Organism

Organism UniProt Comment Textmining
Trichormus variabilis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Trichormus variabilis

Reaction

Reaction Comment Organism Reaction ID
ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg = ADP + phosphate + [L-Asp(4-L-Arg)]n+1 i.e. the first reaction of cyanophycin synthesis, the second reaction is catalysed by activity EC 6.3.2.29, sequence analysis suggests that cyanophycin synthase has two ATP binding sites corresponding to two active sites Trichormus variabilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg a small amount of cyanophycin is required as a primer Trichormus variabilis ADP + phosphate + [L-Asp(4-L-Arg)]n+1 no formation of AMP, [L-Asp(4-L-Arg)]n-Asp is the substrate for the second reaction catalysed by cyanophycin synthase ?
ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg a short peptide is required as a primer, the primer peptide is elongated at its C-terminus Trichormus variabilis ADP + phosphate + [L-Asp(4-L-Arg)]n+1
-
?
L-aspartic acid + ATP
-
Trichormus variabilis poly-L-aspartic acid + ADP + phosphate
-
?
additional information no product is formed if the peptide primer is blocked at the C-terminus or if L-arginine is solely supplied as substrate Trichormus variabilis ?
-
?

Synonyms

Synonyms Comment Organism
CphA
-
Trichormus variabilis
cyanophycin synthetase
-
Trichormus variabilis