6.3.2.30: cyanophycin synthase (L-arginine-adding)
This is an abbreviated version!
For detailed information about cyanophycin synthase (L-arginine-adding), go to the full flat file.
Word Map on EC 6.3.2.30
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6.3.2.30
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synechocystis
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cyanobacteria
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anabaena
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cyanophycinase
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synthesis
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eutropha
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elongatus
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thermosynechococcus
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multi-l-arginyl-poly-l-aspartic
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heterocysts
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nitrogen-rich
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desulfitobacterium
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non-cyanobacterial
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hafniense
- 6.3.2.30
- synechocystis
- cyanobacteria
- anabaena
- cyanophycinase
- synthesis
- eutropha
- elongatus
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thermosynechococcus
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multi-l-arginyl-poly-l-aspartic
- heterocysts
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nitrogen-rich
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desulfitobacterium
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non-cyanobacterial
- hafniense
Reaction
Synonyms
Ava_0335, CGP synthetase, CphA, CphA1, CphA2, CphA49, CphANE1, cyanophycin synthetase, More, Tlr2170 protein
ECTree
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Natural Substrates Products
Natural Substrates Products on EC 6.3.2.30 - cyanophycin synthase (L-arginine-adding)
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REACTION DIAGRAM
ADP + phosphate + [L-Asp(4-L-Arg)]n+1
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ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg
ADP + phosphate + [L-Asp(4-L-Arg)]n+1
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ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg
ADP + phosphate + [L-Asp(4-L-Arg)]n+1
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ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg
ADP + phosphate + [L-Asp(4-L-Arg)]n+1
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ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg
ADP + phosphate + [L-Asp(4-L-Arg)]n+1
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ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg
ADP + phosphate + [L-Asp(4-L-Arg)]n+1
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ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg
ADP + phosphate + [L-Asp(4-L-Arg)]n+1
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ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg
ADP + phosphate + [L-Asp(4-L-Arg)]n+1
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study of expression profiles of the genes cphA1 and cphA2 and their dependence on the type of nitrogen supply in the medium
[L-Asp(4-L-Arg)]n-Asp is the substrate for the second reaction catalysed by cyanophycin synthase
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ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg
ADP + phosphate + [L-Asp(4-L-Arg)]n+1
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ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg
ADP + phosphate + [L-Asp(4-L-Arg)]n+1
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ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg
ADP + phosphate + [L-Asp(4-L-Arg)]n+1
Thermosynechococcus vestitus
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ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg
ADP + phosphate + [L-Asp(4-L-Arg)]n+1
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a small amount of cyanophycin is required as a primer
no formation of AMP, [L-Asp(4-L-Arg)]n-Asp is the substrate for the second reaction catalysed by cyanophycin synthase
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ATP + [L-Asp(4-L-Arg)]n-L-Asp + L-Arg
ADP + phosphate + [L-Asp(4-L-Arg)]n+1
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without L-aspartic acid 9.2% activity compared to the reaction mixture containing both substrates, no activity using L-glutamic acid instead of L-aspartic acid, no activity without addition of small amounts of cyanophycin as a primer for synthesis
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additional information
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Thermosynechococcus vestitus
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CphA requires L-Asp, L-Arg, ATP, Mg2+, and low-molecular mass cyanophycin as a primer for cyanophycin synthesis and catalyzes the elongation of a low-molecular mass cyanophycin, overview
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additional information
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cyanophycin synthetase catalyzes the synthesis of cyanophycin by ATP-dependent polymerization of L-Asp and L-Arg. In vitro, the activity of CphA generally depends on the presence of L-Asp, L-Arg, ATP, Mg2+, K+, sulfhydryl compound, and cyanophycin as primers
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