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6.3.2.30: cyanophycin synthase (L-arginine-adding)

This is an abbreviated version!
For detailed information about cyanophycin synthase (L-arginine-adding), go to the full flat file.

Word Map on EC 6.3.2.30

Reaction

ATP
+
[L-Asp(4-L-Arg)]n-L-Asp
 +
L-Arg
=
ADP
 +
phosphate
 +
[L-Asp(4-L-Arg)]n+1

Synonyms

Ava_0335, CGP synthetase, CphA, CphA1, CphA2, CphA49, CphANE1, cyanophycin synthetase, More, Tlr2170 protein

ECTree

     6 Ligases
         6.3 Forming carbon-nitrogen bonds
             6.3.2 Acid—amino-acid ligases (peptide synthases)
                6.3.2.30 cyanophycin synthase (L-arginine-adding)

Cloned

Cloned on EC 6.3.2.30 - cyanophycin synthase (L-arginine-adding)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
engineered cyanophycin synthetase (CphA) from Nostoc ellipsosporum confers enhanced CphA activity and cyanophycin accumulation to Escherichia coli
expression in Escherichai coli
expression in Escherichia coli
expression in Escherichia coli BL21(DE3)
-
expression in Escherichia coli DH1 cells
-
expression in Escherichia coli DH5alpha
expression in Escherichia coli TOP10 cells
expression in Pseudomonas putida ATCC 4359
expression in the wild-type Sinorhizobium meliloti 1021 and in a phbC-negative mutant
-
functional expression in Nicotiana tabacum var. Petit Havana SRI targeted to the chloroplasts using the CaMV 35S promoter and a translocation pathway signal sequence, the phenotypic abnormalities are reduced by this way, cyanophycin accumulation in chloroplasts, overview
Thermosynechococcus vestitus
-
gene cphA, DNA and amino acid sequence determination and analysis, phylogenetic analysis, functional overexpression of His-tagged Tlr2170 in Escherichia coli BL21(DE3)
Thermosynechococcus vestitus
-
gene cphA, expression in Solanum tuberosum tubers under control of the tuber-specific class 1 promoter B33 directed to tuber cytosol or to tuber plastids leading to cyanophycin accumulation in the tubers
Thermosynechococcus vestitus
-
gene cphA, subcloning and expression of His-tagged wild-type and mutant enzymes in Escherichia coli strains DH5alpha and BL21(DE3)
-
gene cphA49 cloned from deep-sea sediment metagenomic library with degenerated primers, amplified from the fss49 fosmid by PCR, DNA and amino acid sequence determination and analysis, functional expression of His-tagged cphA49 in Escherichia coli strain BL21(DE3), phylogenetic tree, subcloning in Escherichia coli strain DH5alpha
gene cphA6308, expression in Pichia pastoris and Escherichia coli from expression vectors pPICHOLI-C with the copper-inducible CUP1 promoter and pPICHOLI-3 with the methanol-inducible AOX1 promoter. Stabilization of the expression plasmid, the his4 gene from Saccharomyces cerevisiae is introduced and the expression is made in the His auxotrophic Pichia pastoris strain GS115. Recombinant polymer production by wild-type and mutant enzymes and product compositions, overview
-
gene cphA6308, functional expression in Saccharomyces cerevisiae strains G175 and BY4741, which is much more efficient with the copper ion-inducible CUP1 promoter instead of the GAL1 promoter, the yeast strains produce water-soluble and water-insoluble cyanophycin polymer. Growth of transgenic yeasts in the presence of 15 mM lysine results in an incorporation of up to 10 mol% of lysine into cyanophycin, overview. Subcloning in Escherichia coli strain XL1-Blue
-
gene cphA6803, enzyme expression in auxotrophic mutant Rhizopus oryzae strain M16 under control of the pyruvate decarboxylase promoter and terminator elements of Rhizopus oryzae by biolistic transformation method
gene cphA7120, enzyme expression in auxotrophic mutant Rhizopus oryzae strain M16 under control of the pyruvate decarboxylase promoter and terminator elements of Rhizopus oryzae by biolistic transformation method