6.3.2.30: cyanophycin synthase (L-arginine-adding)
This is an abbreviated version!
For detailed information about cyanophycin synthase (L-arginine-adding), go to the full flat file.
Word Map on EC 6.3.2.30
-
6.3.2.30
-
synechocystis
-
cyanobacteria
-
anabaena
-
cyanophycinase
-
synthesis
-
eutropha
-
elongatus
-
thermosynechococcus
-
multi-l-arginyl-poly-l-aspartic
-
heterocysts
-
nitrogen-rich
-
desulfitobacterium
-
non-cyanobacterial
-
hafniense
- 6.3.2.30
- synechocystis
- cyanobacteria
- anabaena
- cyanophycinase
- synthesis
- eutropha
- elongatus
-
thermosynechococcus
-
multi-l-arginyl-poly-l-aspartic
- heterocysts
-
nitrogen-rich
-
desulfitobacterium
-
non-cyanobacterial
- hafniense
Reaction
Synonyms
Ava_0335, CGP synthetase, CphA, CphA1, CphA2, CphA49, CphANE1, cyanophycin synthetase, More, Tlr2170 protein
ECTree
Advanced search results
Engineering
Engineering on EC 6.3.2.30 - cyanophycin synthase (L-arginine-adding)
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
C595S
additional information
C595S
-
site-directed mutagenesis, the mutant shows a 2.5fold increased catalytic activity compared to the wild-type enzyme
two truncated CphAs, lacking 31 (CphANE1del96) or 59 (CphANE1del180) amino acids of the C-terminal region, are derived from cphANE1 by deleting 96 or 180 bp from its 3' region through the introduction of stop codons. In comparison to the wild-type gene, cphANE1del96 conferrs about 2.1-2.2fold higher enzyme activity on Escherichia coli. These cells accumulate about twofold more cyanophycin than cells expressing the wild-type gene
additional information
-
two truncated CphAs, lacking 31 (CphANE1del96) or 59 (CphANE1del180) amino acids of the C-terminal region, are derived from cphANE1 by deleting 96 or 180 bp from its 3' region through the introduction of stop codons. In comparison to the wild-type gene, cphANE1del96 conferrs about 2.1-2.2fold higher enzyme activity on Escherichia coli. These cells accumulate about twofold more cyanophycin than cells expressing the wild-type gene
additional information
-
construction of truncation mutant CphANE1-C45, which lacks up to 45 amino acids at its C-terminus, the mutant retains full enzymatic activity and produced polymers, however, the removal of one additional amino acid, Glu856, results in complete inactivation of CphANE1-C46, phenotype, overview. Removal of the sequence Leu867-Leu870 results in dramatically decreased thermostability
additional information
-
construction of truncation mutant CphANE1-C45, which lacks up to 45 amino acids at its C-terminus, the mutant retains full enzymatic activity and produced polymers, however, the removal of one additional amino acid, Glu856, results in complete inactivation of CphANE1-C46, phenotype, overview. Removal of the sequence Leu867-Leu870 results in dramatically decreased thermostability
-
additional information
-
construction of deletion mutants CphA6308DELTA1, CphA6308DELTA2, and CphA6308DELTA3, which are truncated by one, two, or three amino acid(s), respectively, at the C-terminus, and a combination of the deletion mutation with point mutation C595S, mutants CphA6308DELTA1 and CphA6308DELTA1/C595S show increased catalytic activity compared to the wild-type enzyme, while mutants CphA6308DELTA2 and CphA6308DELTA3 show similar or reduced CphA enzyme activity in comparison to wild-type CphA6308
additional information
Thermosynechococcus vestitus
-
generation of transgenic Solanum tuberosum plants that recombinantly produce the bacterial enzyme by tuber-specific expression. The mutant plants show increased cyanophycin contents, and the tuber-specific expression minimizes the phenotypic abnormalities of the sprout caused by cyanophycin accumulation, phenotypes,overview